Effects of pH on a fluorogenic vital dyes assay (4′,6′-diamidino-2-phenyl-indole and propidium iodide) for Cryptosporidium sp. oocysts

The fluorogenic vital dyes assay utilizing 4′,6′-diamidino-2-phenyl-indole (DAPI) and propidium iodide (PI) can be used for determining the viability of individual Cryptosporidium parvum oocysts isolated from environmental samples; however this assay can be affected by the pH of the oocyst suspendin...

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Veröffentlicht in:	Water research (Oxford) 1999, Vol.33 (13), p.3037-3039
Hauptverfasser:	Bukhari, Z, Glen, W.G, Clancy, J.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Aromatic compounds Assays Biological and medical sciences Cryptosporidium Crystallization crystals DAPI/PI Dyes fluorogenic vital dye assay Fundamental and applied biological sciences. Psychology Microbiology Microorganisms Microscopic examination Monoclonal antibodies Mycological methods and techniques used in mycology Mycology oocysts Salts
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