Continuous cider fermentation with co-immobilized yeast and Leuconostoc oenos cells

Ca-alginate matrix was used to co-immobilize Saccharomyces bayanus and Leuconostoc oenos in one integrated biocatalytic system in order to perform simultaneously alcoholic and malo-lactic fermentation of apple juice to produce cider, in a continuous packed bed bioreactor. The continuous process perm...

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Veröffentlicht in:Enzyme and microbial technology 2000-06, Vol.26 (9-10), p.834-839
Hauptverfasser: Nedovic, V.A, Durieuxb, A, Van Nedervelde, L, Rosseels, P, Vandegans, J, Plaisant, A.-M, Simon, J.-P
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container_end_page 839
container_issue 9-10
container_start_page 834
container_title Enzyme and microbial technology
container_volume 26
creator Nedovic, V.A
Durieuxb, A
Van Nedervelde, L
Rosseels, P
Vandegans, J
Plaisant, A.-M
Simon, J.-P
description Ca-alginate matrix was used to co-immobilize Saccharomyces bayanus and Leuconostoc oenos in one integrated biocatalytic system in order to perform simultaneously alcoholic and malo-lactic fermentation of apple juice to produce cider, in a continuous packed bed bioreactor. The continuous process permitted much faster fermentation compared with the traditional batch process. The flavor formation was also better controlled. By adjusting the flow rate of feeding substrate through the bioreactor, i.e. its residence time, it was possible to obtain either “soft” or “dry” cider. However, the profile of volatile compounds in the final product was modified comparatively to the batch process, especially for higher alcohols, isoamylacetate, and diacetyl. This modification is due to different physiology states of yeast in two processes. Nevertheless, the taste of cider was quite acceptable.
doi_str_mv 10.1016/S0141-0229(00)00179-4
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source Elsevier ScienceDirect Journals
subjects Alcoholic beverages and cider
Batch cell culture
Biological and medical sciences
Cell immobilization
Cider
Co-immobilization
Continuous fermentation
Fermented food industries
Fermenters
Flavor development
Flavors
Food industries
Fundamental and applied biological sciences. Psychology
L. oenos
Packed beds
Yeast
title Continuous cider fermentation with co-immobilized yeast and Leuconostoc oenos cells
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