Characterization of Cell Culture Bed Apatite Sheet and CHO-K1 Cell Static Culture on Apatite Sheet Bed

Characterization of Cell Culture Bed Apatite Sheet and CHO-K1 Cell Static Culture on Apatite Sheet Bed

New type cell culture bed apatite sheet was made from Hydroxyapatite-pulp composite fiber (HAPC) and polyethylene fiber by the method as wide as possible to open surface area using paper making technique. It has become apparent that apatite sheet has good structure for cell culture bed. The sheet ha...

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Veröffentlicht in:Sen'i Gakkaishi 2000, Vol.56(4), pp.205-209
Hauptverfasser: Kawakatsu, Hironobu, Ide, Seiji, Okuda, Yasumasa
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Sprache:eng ; jpn
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Animal cell culture
Plastic filaments
Pulp
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container_title Sen'i Gakkaishi
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creator Kawakatsu, Hironobu
Ide, Seiji
Okuda, Yasumasa
description New type cell culture bed apatite sheet was made from Hydroxyapatite-pulp composite fiber (HAPC) and polyethylene fiber by the method as wide as possible to open surface area using paper making technique. It has become apparent that apatite sheet has good structure for cell culture bed. The sheet has many 10-200μm diameter pores which were formed by ribbon tape shape HAPC. And pore had suitable apatite surface on which mammalian cell grow. These pores linked from top side of sheet to bottom side, and its surface area was 0.32m2/g. CHO-K1 cell were statically cultured in high density on apatite sheet bed which was set in 60mm plastic dish with 7ml Ham F-12 medium includeing 10%FBS. Cell culture bed apatite sheet is expected to be used extensively for cell culture procedure by researcher working in biological industry.
doi_str_mv 10.2115/fiber.56.205
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Animal cell culture
Plastic filaments
Pulp
title Characterization of Cell Culture Bed Apatite Sheet and CHO-K1 Cell Static Culture on Apatite Sheet Bed
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