Studies on cellulase of Lumbricus rubellus (USA)
The water extract of the crude high-protein (68·80%)-containing dry powder of Lumbricus rubellus (USA) was found to contain cellulase, chitinase, protease and amylase. Cellulase activity was present at high levels (940 units/ml) and prompted comparative studies with cellulase from the culture filtra...
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| Veröffentlicht in: | Bioresource technology 1999-08, Vol.69 (2), p.161-165 |
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| creator | Kavian, M.F. Ghatnekar, S.D. Kulkarni, P.R. |
| description | The water extract of the crude high-protein (68·80%)-containing dry powder of
Lumbricus rubellus (USA) was found to contain cellulase, chitinase, protease and amylase. Cellulase activity was present at high levels (940 units/ml) and prompted comparative studies with cellulase from the culture filtrate of the cellulase-rich
Trichoderma viride BRC-25, which was present at 2390 units/ml. A partial purification of the cellulase from both these sources by acetone precipitation followed by ammonium sulphate precipitation at different saturation levels was carried out: 40–80% ammonium sulphate precipitates of both sources had an optimum pH of 6 and temperature of 37°C. Filter paper was the best substrate for both the enzymes. The Km for 40–80% ammonium sulphate fraction for
L. rubellus (USA) and
T. viride BRC-25 enzymes were 9 and 6·75 mg/ml, respectively. |
| doi_str_mv | 10.1016/S0960-8524(98)00159-X |
| format | Article |
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Lumbricus rubellus (USA) was found to contain cellulase, chitinase, protease and amylase. Cellulase activity was present at high levels (940 units/ml) and prompted comparative studies with cellulase from the culture filtrate of the cellulase-rich
Trichoderma viride BRC-25, which was present at 2390 units/ml. A partial purification of the cellulase from both these sources by acetone precipitation followed by ammonium sulphate precipitation at different saturation levels was carried out: 40–80% ammonium sulphate precipitates of both sources had an optimum pH of 6 and temperature of 37°C. Filter paper was the best substrate for both the enzymes. The Km for 40–80% ammonium sulphate fraction for
L. rubellus (USA) and
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Lumbricus rubellus (USA) was found to contain cellulase, chitinase, protease and amylase. Cellulase activity was present at high levels (940 units/ml) and prompted comparative studies with cellulase from the culture filtrate of the cellulase-rich
Trichoderma viride BRC-25, which was present at 2390 units/ml. A partial purification of the cellulase from both these sources by acetone precipitation followed by ammonium sulphate precipitation at different saturation levels was carried out: 40–80% ammonium sulphate precipitates of both sources had an optimum pH of 6 and temperature of 37°C. Filter paper was the best substrate for both the enzymes. The Km for 40–80% ammonium sulphate fraction for
L. rubellus (USA) and
T. viride BRC-25 enzymes were 9 and 6·75 mg/ml, respectively.</description><subject>Acetone</subject><subject>Ammonium compounds</subject><subject>Annelida and closely related phyla: sipuncula. Echiura. Nemertinea</subject><subject>Biological and medical sciences</subject><subject>Biological treatment of sewage sludges and wastes</subject><subject>Biotechnology</subject><subject>Catalyst activity</subject><subject>cellulase</subject><subject>Environment and pollution</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Invertebrates</subject><subject>Lumbricus rubellus (USA)</subject><subject>Precipitation (chemical)</subject><subject>Purification</subject><subject>soil biology</subject><subject>soil science</subject><subject>Substrates</subject><subject>Trichoderma viride BRC-25</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkE1Lw0AQhhdRsFZ_gpiDSHuIzn4l2ZOU4hcUPNRCb8tmdyIraVN3G8F_b9IWPQoDMzDPzLzzEnJJ4ZYCze7moDJIC8nESBVjACpVujwiA1rkPGUqz47J4Bc5JWcxfgAApzkbEJhvW-cxJs06sVjXbW0iJk2VzNpVGbxtYxLasm_EZLSYT8bn5KQydcSLQx6SxePD2_Q5nb0-vUwns9RyRbepKERlsKSSOoPASstLiWXOunClFSwvHBilCuGEZAYzAVx1tZPcoTOV40Nys9-7Cc1ni3GrVz72Cs0amzZqRgVlLJMdKPegDU2MASu9CX5lwremoHt_9M4f3T-vVaF3_uhlN3d9OGCiNXUVzNr6-DecC1ZkvMOu9lhlGm3eQ4cs5gwoB6Ygk6wn7vcEdnZ8eQw6Wo9ri84HtFvtGv-PlB-YSoIn</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>Kavian, M.F.</creator><creator>Ghatnekar, S.D.</creator><creator>Kulkarni, P.R.</creator><general>Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990801</creationdate><title>Studies on cellulase of Lumbricus rubellus (USA)</title><author>Kavian, M.F. ; Ghatnekar, S.D. ; Kulkarni, P.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-484faeb151dae02bc3b5eb72b72dbc4278d0a9984d452ae640394d4d53dedafd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acetone</topic><topic>Ammonium compounds</topic><topic>Annelida and closely related phyla: sipuncula. Echiura. Nemertinea</topic><topic>Biological and medical sciences</topic><topic>Biological treatment of sewage sludges and wastes</topic><topic>Biotechnology</topic><topic>Catalyst activity</topic><topic>cellulase</topic><topic>Environment and pollution</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Invertebrates</topic><topic>Lumbricus rubellus (USA)</topic><topic>Precipitation (chemical)</topic><topic>Purification</topic><topic>soil biology</topic><topic>soil science</topic><topic>Substrates</topic><topic>Trichoderma viride BRC-25</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kavian, M.F.</creatorcontrib><creatorcontrib>Ghatnekar, S.D.</creatorcontrib><creatorcontrib>Kulkarni, P.R.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kavian, M.F.</au><au>Ghatnekar, S.D.</au><au>Kulkarni, P.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on cellulase of Lumbricus rubellus (USA)</atitle><jtitle>Bioresource technology</jtitle><date>1999-08-01</date><risdate>1999</risdate><volume>69</volume><issue>2</issue><spage>161</spage><epage>165</epage><pages>161-165</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>The water extract of the crude high-protein (68·80%)-containing dry powder of
Lumbricus rubellus (USA) was found to contain cellulase, chitinase, protease and amylase. Cellulase activity was present at high levels (940 units/ml) and prompted comparative studies with cellulase from the culture filtrate of the cellulase-rich
Trichoderma viride BRC-25, which was present at 2390 units/ml. A partial purification of the cellulase from both these sources by acetone precipitation followed by ammonium sulphate precipitation at different saturation levels was carried out: 40–80% ammonium sulphate precipitates of both sources had an optimum pH of 6 and temperature of 37°C. Filter paper was the best substrate for both the enzymes. The Km for 40–80% ammonium sulphate fraction for
L. rubellus (USA) and
T. viride BRC-25 enzymes were 9 and 6·75 mg/ml, respectively.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0960-8524(98)00159-X</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-8524 |
| ispartof | Bioresource technology, 1999-08, Vol.69 (2), p.161-165 |
| issn | 0960-8524 1873-2976 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Acetone Ammonium compounds Annelida and closely related phyla: sipuncula. Echiura. Nemertinea Biological and medical sciences Biological treatment of sewage sludges and wastes Biotechnology Catalyst activity cellulase Environment and pollution Fundamental and applied biological sciences. Psychology Industrial applications and implications. Economical aspects Invertebrates Lumbricus rubellus (USA) Precipitation (chemical) Purification soil biology soil science Substrates Trichoderma viride BRC-25 |
| title | Studies on cellulase of Lumbricus rubellus (USA) |
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