Organophosphorus hydrolase-based assay for organophosphate pesticides
We report a rapid and versatile organophosphorus hydrolase (OPH) ‐based method for measurement of organophosphates. This assay is based on a substrate‐dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently i...
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| Veröffentlicht in: | Biotechnology progress 1999, Vol.15 (3), p.517-521 |
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| container_title | Biotechnology progress |
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| creator | Rogers, K.R Wang, Y Mulchandani, A Mulchandani, P Chen, W |
| description | We report a rapid and versatile organophosphorus hydrolase (OPH) ‐based method for measurement of organophosphates. This assay is based on a substrate‐dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which the FITC‐labeled enzyme is adsorbed. Analytes were then measured using a microbead fluorescence analyzer. The dynamic concentration range for the assay extends from 25 to 400 μM for paraoxon with a detection limit of 8 μM. Organophosphorus insecticides measured using this technique included ethylparathion, methylparathion, dursban, fensulfothion, crotoxyphos, diazinon, mevinphos, dichlorvos, and coumaphos. This technique was used to measure coumaphos in biodegradation samples of cattle dip wastes and showed a high correlation (r2 = 0.998) to an HPLC method. |
| doi_str_mv | 10.1021/bp990034e |
| format | Article |
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This assay is based on a substrate‐dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which the FITC‐labeled enzyme is adsorbed. Analytes were then measured using a microbead fluorescence analyzer. The dynamic concentration range for the assay extends from 25 to 400 μM for paraoxon with a detection limit of 8 μM. Organophosphorus insecticides measured using this technique included ethylparathion, methylparathion, dursban, fensulfothion, crotoxyphos, diazinon, mevinphos, dichlorvos, and coumaphos. 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Economical aspects ; organophosphorus insecticides ; Pesticides ; pH effects ; Polymethyl methacrylates</subject><ispartof>Biotechnology progress, 1999, Vol.15 (3), p.517-521</ispartof><rights>Copyright © 1999 American Institute of Chemical Engineers (AIChE)</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4784-7fd62db12961c19b1ae013c45baf7d815aeb438fd57539a6dc67077fce17cc743</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1021%2Fbp990034e$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1021%2Fbp990034e$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,4010,27900,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1873999$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10356272$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rogers, K.R</creatorcontrib><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Mulchandani, A</creatorcontrib><creatorcontrib>Mulchandani, P</creatorcontrib><creatorcontrib>Chen, W</creatorcontrib><title>Organophosphorus hydrolase-based assay for organophosphate pesticides</title><title>Biotechnology progress</title><addtitle>Biotechnol Progress</addtitle><description>We report a rapid and versatile organophosphorus hydrolase (OPH) ‐based method for measurement of organophosphates. This assay is based on a substrate‐dependent change in pH at the local vicinity of the enzyme. The pH change is monitored using fluorescein isothiocyanate (FITC), which is covalently immobilized to the enzyme. This method employs the use of poly(methyl methacrylate) beads to which the FITC‐labeled enzyme is adsorbed. Analytes were then measured using a microbead fluorescence analyzer. The dynamic concentration range for the assay extends from 25 to 400 μM for paraoxon with a detection limit of 8 μM. Organophosphorus insecticides measured using this technique included ethylparathion, methylparathion, dursban, fensulfothion, crotoxyphos, diazinon, mevinphos, dichlorvos, and coumaphos. This technique was used to measure coumaphos in biodegradation samples of cattle dip wastes and showed a high correlation (r2 = 0.998) to an HPLC method.</description><subject>Bioassay</subject><subject>Biodegradation</subject><subject>Biodegradation of pollutants</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Environment and pollution</subject><subject>Enzyme immobilization</subject><subject>Enzyme kinetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>immobilization</subject><subject>immobilized enzymes</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>organophosphorus insecticides</subject><subject>Pesticides</subject><subject>pH effects</subject><subject>Polymethyl methacrylates</subject><issn>8756-7938</issn><issn>1520-6033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqF0Utv1DAQAGALUdGlcOAPQA4I0UPA42d8pFVpESuK-lCP1sR22kB2Hexdwf57vMqq9AIc_Dh889AMIS-AvgPK4H07GkMpF-ERmYFktFaU88dk1mipam14s0-e5vyNUtpQxZ6QfaBcKqbZjJycp1tcxvEu5nLSOld3G5_igDnUbbl8hTnjpupiquIDiqtQjSGvetf7kJ-RvQ6HHJ7v3gNy_fHk6visnp-ffjr-MK-d0I2odecV8y0wo8CBaQEDBe6EbLHTvgGJoRW86bzUkhtU3ilNte5cAO2cFvyAvJnyjin-WJfydtFnF4YBlyGus2UgABhTBb79J4RGGk6VZOa_OUEzUea5hYcTdCnmnEJnx9QvMG0sULvdg73fQ7Evd0nX7SL4B3IafAGvdwCzw6FLuHR9_uMazY3Z1oSJ_eyHsPl7QXt09fVi-peYeorp8yr8uo_B9N0qzbW0N19O7eXFZzU_O7qxuvhXk-8wWrxNpY3rS1YWQ5kBEEzw30UwtwQ</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>Rogers, K.R</creator><creator>Wang, Y</creator><creator>Mulchandani, A</creator><creator>Mulchandani, P</creator><creator>Chen, W</creator><general>American Chemical Society</general><general>American Institute of Chemical Engineers</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Organophosphorus hydrolase-based assay for organophosphate pesticides</title><author>Rogers, K.R ; Wang, Y ; Mulchandani, A ; Mulchandani, P ; Chen, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4784-7fd62db12961c19b1ae013c45baf7d815aeb438fd57539a6dc67077fce17cc743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bioassay</topic><topic>Biodegradation</topic><topic>Biodegradation of pollutants</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Environment and pollution</topic><topic>Enzyme immobilization</topic><topic>Enzyme kinetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>immobilization</topic><topic>immobilized enzymes</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>organophosphorus insecticides</topic><topic>Pesticides</topic><topic>pH effects</topic><topic>Polymethyl methacrylates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rogers, K.R</creatorcontrib><creatorcontrib>Wang, Y</creatorcontrib><creatorcontrib>Mulchandani, A</creatorcontrib><creatorcontrib>Mulchandani, P</creatorcontrib><creatorcontrib>Chen, W</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology progress</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rogers, K.R</au><au>Wang, Y</au><au>Mulchandani, A</au><au>Mulchandani, P</au><au>Chen, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organophosphorus hydrolase-based assay for organophosphate pesticides</atitle><jtitle>Biotechnology progress</jtitle><addtitle>Biotechnol Progress</addtitle><date>1999</date><risdate>1999</risdate><volume>15</volume><issue>3</issue><spage>517</spage><epage>521</epage><pages>517-521</pages><issn>8756-7938</issn><eissn>1520-6033</eissn><coden>BIPRET</coden><abstract>We report a rapid and versatile organophosphorus hydrolase (OPH) ‐based method for measurement of organophosphates. 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| ispartof | Biotechnology progress, 1999, Vol.15 (3), p.517-521 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Bioassay Biodegradation Biodegradation of pollutants Biological and medical sciences Biotechnology Environment and pollution Enzyme immobilization Enzyme kinetics Fundamental and applied biological sciences. Psychology Hydrolysis immobilization immobilized enzymes Industrial applications and implications. Economical aspects organophosphorus insecticides Pesticides pH effects Polymethyl methacrylates |
| title | Organophosphorus hydrolase-based assay for organophosphate pesticides |
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