Developmental and age-related changes to the elastic lamina of Bruch’s membrane in mice
Background Fibrillin-1, tropoelastin, fibulin-5, and latent transforming growth factor beta-binding protein-2 and protein-4 (LTBP-2 and LTBP-4) are essential proteins for the elastic lamina (EL). In this study, we analyzed each of these molecules in the EL of Bruch’s membrane (BM) through developmen...
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| Veröffentlicht in: | Graefe's archive for clinical and experimental ophthalmology 2019-02, Vol.257 (2), p.289-301 |
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| creator | Mori, Hidetsugu Yamada, Haruhiko Toyama, Keiko Takahashi, Kanji Akama, Tomoya Inoue, Tadashi Nakamura, Tomoyuki |
| description | Background
Fibrillin-1, tropoelastin, fibulin-5, and latent transforming growth factor beta-binding protein-2 and protein-4 (LTBP-2 and LTBP-4) are essential proteins for the elastic lamina (EL). In this study, we analyzed each of these molecules in the EL of Bruch’s membrane (BM) through development and aging.
Methods
C57BL/6 mice (embryonic (E) days E12.5, E15.5, and E18.5; postnatal (P) days P1, P4, and P7 and P3, P6, and P75 weeks of age) were used. To investigate localization, immunohistochemical staining (IH) was performed. Transmission electron microscopy (TEM) was used to evaluate the formation of microfibrils and tropoelastin. mRNA expression was determined by quantitative real-time PCR (qRT-PCR).
Results
All five proteins were expressed in the EL of BM by IH except in embryonic mice. TEM results showed that tropoelastin co-stained with microfibrils. Between 3 and 6 weeks of age, microfibrils became longer and thicker. It was difficult to evaluate the EL of BM in senile mice at 75 weeks of age because of abundant deposits which correspond to drusen. mRNA levels of each protein increased dramatically from E15.5 to P1 days and plateaued by P3 weeks as shown by qRT-PCR.
Conclusions
In conclusion, these five proteins are possibly involved in elastic fiber assembly in BM. We define the date of full assembly of the EL of BM as 3 weeks of age in mice. |
| doi_str_mv | 10.1007/s00417-018-4184-5 |
| format | Article |
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Fibrillin-1, tropoelastin, fibulin-5, and latent transforming growth factor beta-binding protein-2 and protein-4 (LTBP-2 and LTBP-4) are essential proteins for the elastic lamina (EL). In this study, we analyzed each of these molecules in the EL of Bruch’s membrane (BM) through development and aging.
Methods
C57BL/6 mice (embryonic (E) days E12.5, E15.5, and E18.5; postnatal (P) days P1, P4, and P7 and P3, P6, and P75 weeks of age) were used. To investigate localization, immunohistochemical staining (IH) was performed. Transmission electron microscopy (TEM) was used to evaluate the formation of microfibrils and tropoelastin. mRNA expression was determined by quantitative real-time PCR (qRT-PCR).
Results
All five proteins were expressed in the EL of BM by IH except in embryonic mice. TEM results showed that tropoelastin co-stained with microfibrils. Between 3 and 6 weeks of age, microfibrils became longer and thicker. It was difficult to evaluate the EL of BM in senile mice at 75 weeks of age because of abundant deposits which correspond to drusen. mRNA levels of each protein increased dramatically from E15.5 to P1 days and plateaued by P3 weeks as shown by qRT-PCR.
Conclusions
In conclusion, these five proteins are possibly involved in elastic fiber assembly in BM. We define the date of full assembly of the EL of BM as 3 weeks of age in mice.</description><identifier>ISSN: 0721-832X</identifier><identifier>EISSN: 1435-702X</identifier><identifier>DOI: 10.1007/s00417-018-4184-5</identifier><identifier>PMID: 30498857</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Age ; Aging ; Basic Science ; Embryogenesis ; Fibrillin ; Gene expression ; Localization ; Medicine ; Medicine & Public Health ; Microfibrils ; Ophthalmology ; Proteins ; Transmission electron microscopy ; Tropoelastin</subject><ispartof>Graefe's archive for clinical and experimental ophthalmology, 2019-02, Vol.257 (2), p.289-301</ispartof><rights>The Author(s) 2018</rights><rights>Graefe's Archive for Clinical and Experimental Ophthalmology is a copyright of Springer, (2018). All Rights Reserved. © 2018. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p212t-e3893984ecdea88bc4f3e03a31a670549e35c5dae52e0208270d5c1490487cb93</cites><orcidid>0000-0002-9966-2287</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00417-018-4184-5$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00417-018-4184-5$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,778,782,27911,27912,41475,42544,51306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30498857$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mori, Hidetsugu</creatorcontrib><creatorcontrib>Yamada, Haruhiko</creatorcontrib><creatorcontrib>Toyama, Keiko</creatorcontrib><creatorcontrib>Takahashi, Kanji</creatorcontrib><creatorcontrib>Akama, Tomoya</creatorcontrib><creatorcontrib>Inoue, Tadashi</creatorcontrib><creatorcontrib>Nakamura, Tomoyuki</creatorcontrib><title>Developmental and age-related changes to the elastic lamina of Bruch’s membrane in mice</title><title>Graefe's archive for clinical and experimental ophthalmology</title><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><description>Background
Fibrillin-1, tropoelastin, fibulin-5, and latent transforming growth factor beta-binding protein-2 and protein-4 (LTBP-2 and LTBP-4) are essential proteins for the elastic lamina (EL). In this study, we analyzed each of these molecules in the EL of Bruch’s membrane (BM) through development and aging.
Methods
C57BL/6 mice (embryonic (E) days E12.5, E15.5, and E18.5; postnatal (P) days P1, P4, and P7 and P3, P6, and P75 weeks of age) were used. To investigate localization, immunohistochemical staining (IH) was performed. Transmission electron microscopy (TEM) was used to evaluate the formation of microfibrils and tropoelastin. mRNA expression was determined by quantitative real-time PCR (qRT-PCR).
Results
All five proteins were expressed in the EL of BM by IH except in embryonic mice. TEM results showed that tropoelastin co-stained with microfibrils. Between 3 and 6 weeks of age, microfibrils became longer and thicker. It was difficult to evaluate the EL of BM in senile mice at 75 weeks of age because of abundant deposits which correspond to drusen. mRNA levels of each protein increased dramatically from E15.5 to P1 days and plateaued by P3 weeks as shown by qRT-PCR.
Conclusions
In conclusion, these five proteins are possibly involved in elastic fiber assembly in BM. We define the date of full assembly of the EL of BM as 3 weeks of age in mice.</description><subject>Age</subject><subject>Aging</subject><subject>Basic Science</subject><subject>Embryogenesis</subject><subject>Fibrillin</subject><subject>Gene expression</subject><subject>Localization</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microfibrils</subject><subject>Ophthalmology</subject><subject>Proteins</subject><subject>Transmission electron microscopy</subject><subject>Tropoelastin</subject><issn>0721-832X</issn><issn>1435-702X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkctKA0EQRRtRTIx-gBtpcOOmtfqV6VlqfILgRiGump6eSjJhHnF6RnDnb_h7fokdEhFcFVQdLpc6hBxzOOcAyUUAUDxhwA1T3Cimd8iQK6lZAmK6S4aQCM6MFNMBOQhhCRGXmu-TgQSVGqOTIXm9xncsm1WFdedK6uqcujmyFkvXYU79wtVzDLRraLdAGrehKzwtXVXUjjYzetX2fvH9-RVohVXWuhppUdOq8HhI9mauDHi0nSPycnvzPLlnj093D5PLR7YSXHQMpUllahT6HJ0xmVcziSCd5G6cgFYpSu117lALBAFGJJBrz1UKyiQ-S-WInG1yV23z1mPobFUEj2UZuzR9sIIrDkrxlEf09B-6bPq2ju0iJVM5NlqsA0-2VJ9VmNtVW1Su_bC_T4uA2AAhnuJ72r8YDnZtxm7M2GjGrs1YLX8AuVp90g</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Mori, Hidetsugu</creator><creator>Yamada, Haruhiko</creator><creator>Toyama, Keiko</creator><creator>Takahashi, Kanji</creator><creator>Akama, Tomoya</creator><creator>Inoue, Tadashi</creator><creator>Nakamura, Tomoyuki</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>NPM</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9966-2287</orcidid></search><sort><creationdate>20190201</creationdate><title>Developmental and age-related changes to the elastic lamina of Bruch’s membrane in mice</title><author>Mori, Hidetsugu ; Yamada, Haruhiko ; Toyama, Keiko ; Takahashi, Kanji ; Akama, Tomoya ; Inoue, Tadashi ; Nakamura, Tomoyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p212t-e3893984ecdea88bc4f3e03a31a670549e35c5dae52e0208270d5c1490487cb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Age</topic><topic>Aging</topic><topic>Basic Science</topic><topic>Embryogenesis</topic><topic>Fibrillin</topic><topic>Gene expression</topic><topic>Localization</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microfibrils</topic><topic>Ophthalmology</topic><topic>Proteins</topic><topic>Transmission electron microscopy</topic><topic>Tropoelastin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mori, Hidetsugu</creatorcontrib><creatorcontrib>Yamada, Haruhiko</creatorcontrib><creatorcontrib>Toyama, Keiko</creatorcontrib><creatorcontrib>Takahashi, Kanji</creatorcontrib><creatorcontrib>Akama, Tomoya</creatorcontrib><creatorcontrib>Inoue, Tadashi</creatorcontrib><creatorcontrib>Nakamura, Tomoyuki</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mori, Hidetsugu</au><au>Yamada, Haruhiko</au><au>Toyama, Keiko</au><au>Takahashi, Kanji</au><au>Akama, Tomoya</au><au>Inoue, Tadashi</au><au>Nakamura, Tomoyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Developmental and age-related changes to the elastic lamina of Bruch’s membrane in mice</atitle><jtitle>Graefe's archive for clinical and experimental ophthalmology</jtitle><stitle>Graefes Arch Clin Exp Ophthalmol</stitle><addtitle>Graefes Arch Clin Exp Ophthalmol</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>257</volume><issue>2</issue><spage>289</spage><epage>301</epage><pages>289-301</pages><issn>0721-832X</issn><eissn>1435-702X</eissn><abstract>Background
Fibrillin-1, tropoelastin, fibulin-5, and latent transforming growth factor beta-binding protein-2 and protein-4 (LTBP-2 and LTBP-4) are essential proteins for the elastic lamina (EL). In this study, we analyzed each of these molecules in the EL of Bruch’s membrane (BM) through development and aging.
Methods
C57BL/6 mice (embryonic (E) days E12.5, E15.5, and E18.5; postnatal (P) days P1, P4, and P7 and P3, P6, and P75 weeks of age) were used. To investigate localization, immunohistochemical staining (IH) was performed. Transmission electron microscopy (TEM) was used to evaluate the formation of microfibrils and tropoelastin. mRNA expression was determined by quantitative real-time PCR (qRT-PCR).
Results
All five proteins were expressed in the EL of BM by IH except in embryonic mice. TEM results showed that tropoelastin co-stained with microfibrils. Between 3 and 6 weeks of age, microfibrils became longer and thicker. It was difficult to evaluate the EL of BM in senile mice at 75 weeks of age because of abundant deposits which correspond to drusen. mRNA levels of each protein increased dramatically from E15.5 to P1 days and plateaued by P3 weeks as shown by qRT-PCR.
Conclusions
In conclusion, these five proteins are possibly involved in elastic fiber assembly in BM. We define the date of full assembly of the EL of BM as 3 weeks of age in mice.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30498857</pmid><doi>10.1007/s00417-018-4184-5</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-9966-2287</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Age Aging Basic Science Embryogenesis Fibrillin Gene expression Localization Medicine Medicine & Public Health Microfibrils Ophthalmology Proteins Transmission electron microscopy Tropoelastin |
| title | Developmental and age-related changes to the elastic lamina of Bruch’s membrane in mice |
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