A new ovine model of spine and chest wall deformity at birth with alteration of respiratory system mechanics and lung development: a feasibility study
Purpose To develop an animal model of spine and chest wall deformity (CWD) at birth and to evaluate its effects on respiratory system mechanics and lung development. Methods A spine and CWD was created in utero between 70 and 75 days of gestation in six ovine fetuses by resection of the seventh and...
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| Veröffentlicht in: | European spine journal 2019-01, Vol.28 (1), p.114-120 |
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| creator | Parent, Stefan Samson, Nathalie Shen, Jesse Gutman, Gabriel Bouchard, Sarah Piedboeuf, Bruno Praud, Jean-Paul |
| description | Purpose
To develop an animal model of spine and chest wall deformity (CWD) at birth and to evaluate its effects on respiratory system mechanics and lung development.
Methods
A spine and CWD was created in utero between 70 and 75 days of gestation in six ovine fetuses by resection of the seventh and eighth left ribs. Two days after birth, respiratory system mechanics was assessed in anesthetized lambs using the flexiVent apparatus, followed by postmortem measurement of lung mechanics as well as histological lung analysis.
Results
A range of severity of CWD was found (Cobb angle from 0° to 48°) with a mean decrease in compliance of 47% and in inspiratory capacity of 39% compared to control lambs. Proof-of-concept histological analysis in one lamb showed marked lung hypoplasia.
Conclusion
Our ovine model represents a pilot proof-of-concept study evaluating the impact of a spine and CWD present at birth on lung respiratory mechanics and development. This study lays down the groundwork for future studies evaluating the impact of these deformities on lung development and potential treatments.
Graphical abstract
These slides can be retrieved under Electronic Supplementary Material. |
| doi_str_mv | 10.1007/s00586-018-5818-3 |
| format | Article |
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To develop an animal model of spine and chest wall deformity (CWD) at birth and to evaluate its effects on respiratory system mechanics and lung development.
Methods
A spine and CWD was created in utero between 70 and 75 days of gestation in six ovine fetuses by resection of the seventh and eighth left ribs. Two days after birth, respiratory system mechanics was assessed in anesthetized lambs using the flexiVent apparatus, followed by postmortem measurement of lung mechanics as well as histological lung analysis.
Results
A range of severity of CWD was found (Cobb angle from 0° to 48°) with a mean decrease in compliance of 47% and in inspiratory capacity of 39% compared to control lambs. Proof-of-concept histological analysis in one lamb showed marked lung hypoplasia.
Conclusion
Our ovine model represents a pilot proof-of-concept study evaluating the impact of a spine and CWD present at birth on lung respiratory mechanics and development. This study lays down the groundwork for future studies evaluating the impact of these deformities on lung development and potential treatments.
Graphical abstract
These slides can be retrieved under Electronic Supplementary Material.</description><identifier>ISSN: 0940-6719</identifier><identifier>EISSN: 1432-0932</identifier><identifier>DOI: 10.1007/s00586-018-5818-3</identifier><identifier>PMID: 30498959</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Birth ; Chest ; Disease Models, Animal ; Feasibility studies ; Female ; Fetus - abnormalities ; Fetus - physiopathology ; Fetuses ; Gestation ; Hypoplasia ; Lungs ; Mechanics ; Medicine ; Medicine & Public Health ; Musculoskeletal Abnormalities - physiopathology ; Neurosurgery ; Original Article ; Pregnancy ; Range of Motion, Articular ; Respiration ; Respiratory Mechanics ; Respiratory system ; Sheep ; Spine ; Spine - abnormalities ; Spine - physiopathology ; Surgical Orthopedics ; Thoracic Wall - abnormalities ; Thoracic Wall - physiopathology</subject><ispartof>European spine journal, 2019-01, Vol.28 (1), p.114-120</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>European Spine Journal is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-305a3dab53d9f3c827e9d8af8ac287d32c7f6017fc9ce36489436e041585593c3</citedby><cites>FETCH-LOGICAL-c415t-305a3dab53d9f3c827e9d8af8ac287d32c7f6017fc9ce36489436e041585593c3</cites><orcidid>0000-0002-4854-2816</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00586-018-5818-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00586-018-5818-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30498959$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parent, Stefan</creatorcontrib><creatorcontrib>Samson, Nathalie</creatorcontrib><creatorcontrib>Shen, Jesse</creatorcontrib><creatorcontrib>Gutman, Gabriel</creatorcontrib><creatorcontrib>Bouchard, Sarah</creatorcontrib><creatorcontrib>Piedboeuf, Bruno</creatorcontrib><creatorcontrib>Praud, Jean-Paul</creatorcontrib><title>A new ovine model of spine and chest wall deformity at birth with alteration of respiratory system mechanics and lung development: a feasibility study</title><title>European spine journal</title><addtitle>Eur Spine J</addtitle><addtitle>Eur Spine J</addtitle><description>Purpose
To develop an animal model of spine and chest wall deformity (CWD) at birth and to evaluate its effects on respiratory system mechanics and lung development.
Methods
A spine and CWD was created in utero between 70 and 75 days of gestation in six ovine fetuses by resection of the seventh and eighth left ribs. Two days after birth, respiratory system mechanics was assessed in anesthetized lambs using the flexiVent apparatus, followed by postmortem measurement of lung mechanics as well as histological lung analysis.
Results
A range of severity of CWD was found (Cobb angle from 0° to 48°) with a mean decrease in compliance of 47% and in inspiratory capacity of 39% compared to control lambs. Proof-of-concept histological analysis in one lamb showed marked lung hypoplasia.
Conclusion
Our ovine model represents a pilot proof-of-concept study evaluating the impact of a spine and CWD present at birth on lung respiratory mechanics and development. This study lays down the groundwork for future studies evaluating the impact of these deformities on lung development and potential treatments.
Graphical abstract
These slides can be retrieved under Electronic Supplementary Material.</description><subject>Animals</subject><subject>Birth</subject><subject>Chest</subject><subject>Disease Models, Animal</subject><subject>Feasibility studies</subject><subject>Female</subject><subject>Fetus - abnormalities</subject><subject>Fetus - physiopathology</subject><subject>Fetuses</subject><subject>Gestation</subject><subject>Hypoplasia</subject><subject>Lungs</subject><subject>Mechanics</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Musculoskeletal Abnormalities - physiopathology</subject><subject>Neurosurgery</subject><subject>Original Article</subject><subject>Pregnancy</subject><subject>Range of Motion, Articular</subject><subject>Respiration</subject><subject>Respiratory Mechanics</subject><subject>Respiratory system</subject><subject>Sheep</subject><subject>Spine</subject><subject>Spine - abnormalities</subject><subject>Spine - physiopathology</subject><subject>Surgical Orthopedics</subject><subject>Thoracic Wall - abnormalities</subject><subject>Thoracic Wall - physiopathology</subject><issn>0940-6719</issn><issn>1432-0932</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kUFvFCEYhonR2G31B3gxJF68jMIwzIC3pqnWpEkv7Zmw8E2XhoEVmG7mj_h7ZdyqiUkPQAjP-wB5EXpHySdKyPA5E8JF3xAqGi7qxF6gDe1Y2xDJ2pdoQ2RHmn6g8gSd5vxACOWS9K_RCSOdFJLLDfp5jgMccHx0AfAULXgcR5z361YHi80OcsEH7T22MMY0ubJgXfDWpbLDB1cn7QskXVwMazRBDddtTAvOSy4w4QnMTgdn8m-jn8N9dT2Cj_sJQvmCNR5BZ7d1fpXnMtvlDXo1ap_h7dN6hu6-Xt5eXDXXN9--X5xfN6ajvDSMcM2s3nJm5ciMaAeQVuhRaNOKwbLWDGNP6DAaaYD1nZAd64HUrOBcMsPO0Mejd5_ij7l-VU0uG_BeB4hzVi3tKOnq6Cv64T_0Ic4p1NdViknWV6OoFD1SJsWcE4xqn9yk06IoUWtp6liaqqWptTTFaub9k3neTmD_Jv60VIH2COR6FO4h_bv6eesvTOej2g</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Parent, Stefan</creator><creator>Samson, Nathalie</creator><creator>Shen, Jesse</creator><creator>Gutman, Gabriel</creator><creator>Bouchard, Sarah</creator><creator>Piedboeuf, Bruno</creator><creator>Praud, Jean-Paul</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4854-2816</orcidid></search><sort><creationdate>20190101</creationdate><title>A new ovine model of spine and chest wall deformity at birth with alteration of respiratory system mechanics and lung development: a feasibility study</title><author>Parent, Stefan ; Samson, Nathalie ; Shen, Jesse ; Gutman, Gabriel ; Bouchard, Sarah ; Piedboeuf, Bruno ; Praud, Jean-Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-305a3dab53d9f3c827e9d8af8ac287d32c7f6017fc9ce36489436e041585593c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Birth</topic><topic>Chest</topic><topic>Disease Models, Animal</topic><topic>Feasibility studies</topic><topic>Female</topic><topic>Fetus - abnormalities</topic><topic>Fetus - physiopathology</topic><topic>Fetuses</topic><topic>Gestation</topic><topic>Hypoplasia</topic><topic>Lungs</topic><topic>Mechanics</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Musculoskeletal Abnormalities - physiopathology</topic><topic>Neurosurgery</topic><topic>Original Article</topic><topic>Pregnancy</topic><topic>Range of Motion, Articular</topic><topic>Respiration</topic><topic>Respiratory Mechanics</topic><topic>Respiratory system</topic><topic>Sheep</topic><topic>Spine</topic><topic>Spine - abnormalities</topic><topic>Spine - physiopathology</topic><topic>Surgical Orthopedics</topic><topic>Thoracic Wall - abnormalities</topic><topic>Thoracic Wall - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parent, Stefan</creatorcontrib><creatorcontrib>Samson, Nathalie</creatorcontrib><creatorcontrib>Shen, Jesse</creatorcontrib><creatorcontrib>Gutman, Gabriel</creatorcontrib><creatorcontrib>Bouchard, Sarah</creatorcontrib><creatorcontrib>Piedboeuf, Bruno</creatorcontrib><creatorcontrib>Praud, Jean-Paul</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>European spine journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parent, Stefan</au><au>Samson, Nathalie</au><au>Shen, Jesse</au><au>Gutman, Gabriel</au><au>Bouchard, Sarah</au><au>Piedboeuf, Bruno</au><au>Praud, Jean-Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A new ovine model of spine and chest wall deformity at birth with alteration of respiratory system mechanics and lung development: a feasibility study</atitle><jtitle>European spine journal</jtitle><stitle>Eur Spine J</stitle><addtitle>Eur Spine J</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>28</volume><issue>1</issue><spage>114</spage><epage>120</epage><pages>114-120</pages><issn>0940-6719</issn><eissn>1432-0932</eissn><abstract>Purpose
To develop an animal model of spine and chest wall deformity (CWD) at birth and to evaluate its effects on respiratory system mechanics and lung development.
Methods
A spine and CWD was created in utero between 70 and 75 days of gestation in six ovine fetuses by resection of the seventh and eighth left ribs. Two days after birth, respiratory system mechanics was assessed in anesthetized lambs using the flexiVent apparatus, followed by postmortem measurement of lung mechanics as well as histological lung analysis.
Results
A range of severity of CWD was found (Cobb angle from 0° to 48°) with a mean decrease in compliance of 47% and in inspiratory capacity of 39% compared to control lambs. Proof-of-concept histological analysis in one lamb showed marked lung hypoplasia.
Conclusion
Our ovine model represents a pilot proof-of-concept study evaluating the impact of a spine and CWD present at birth on lung respiratory mechanics and development. This study lays down the groundwork for future studies evaluating the impact of these deformities on lung development and potential treatments.
Graphical abstract
These slides can be retrieved under Electronic Supplementary Material.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30498959</pmid><doi>10.1007/s00586-018-5818-3</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-4854-2816</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0940-6719 |
| ispartof | European spine journal, 2019-01, Vol.28 (1), p.114-120 |
| issn | 0940-6719 1432-0932 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Birth Chest Disease Models, Animal Feasibility studies Female Fetus - abnormalities Fetus - physiopathology Fetuses Gestation Hypoplasia Lungs Mechanics Medicine Medicine & Public Health Musculoskeletal Abnormalities - physiopathology Neurosurgery Original Article Pregnancy Range of Motion, Articular Respiration Respiratory Mechanics Respiratory system Sheep Spine Spine - abnormalities Spine - physiopathology Surgical Orthopedics Thoracic Wall - abnormalities Thoracic Wall - physiopathology |
| title | A new ovine model of spine and chest wall deformity at birth with alteration of respiratory system mechanics and lung development: a feasibility study |
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