Porous poly( l-lactide) films obtained by immersion precipitation process: morphology, phase separation and culture of VERO cells
In this work, the immersion precipitation process was used to obtain porous poly( l-lactide) films which were tested as supports for culture of VERO cells. The mechanism of phase separation taking place during the film formation was investigated using light scattering measurements. A theoretical ter...
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| Veröffentlicht in: | Polymer (Guilford) 1999-06, Vol.40 (12), p.3275-3289 |
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| container_title | Polymer (Guilford) |
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| creator | Zoppi, R.A Contant, S Duek, E.A.R Marques, F.R Wada, M.L.F Nunes, S.P |
| description | In this work, the immersion precipitation process was used to obtain porous poly(
l-lactide) films which were tested as supports for culture of VERO cells. The mechanism of phase separation taking place during the film formation was investigated using light scattering measurements. A theoretical ternary phase diagram was proposed and correlated with the light scattering results. The film morphology was investigated using scanning electron microscopy. For films with a more porous surface, VERO cells grew with a round shape. For films with a lower surface porosity, the growth of flatter cells was observed. These preliminary tests show that the film morphology can affect the growth of cells, or can further change the cells' functions. |
| doi_str_mv | 10.1016/S0032-3861(98)00562-X |
| format | Article |
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l-lactide) films which were tested as supports for culture of VERO cells. The mechanism of phase separation taking place during the film formation was investigated using light scattering measurements. A theoretical ternary phase diagram was proposed and correlated with the light scattering results. The film morphology was investigated using scanning electron microscopy. For films with a more porous surface, VERO cells grew with a round shape. For films with a lower surface porosity, the growth of flatter cells was observed. These preliminary tests show that the film morphology can affect the growth of cells, or can further change the cells' functions.</description><subject>Animal cells</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Exact sciences and technology</subject><subject>Film morphology</subject><subject>Forms of application and semi-finished materials</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Light scattering</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Morphology</topic><topic>Phase diagrams</topic><topic>Phase separation</topic><topic>Poly( l-lactide)</topic><topic>Polyesters</topic><topic>Polymer industry, paints, wood</topic><topic>Porosity</topic><topic>Precipitation (chemical)</topic><topic>Reaction kinetics</topic><topic>Scanning electron microscopy</topic><topic>Sheets and films</topic><topic>Technology of polymers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zoppi, R.A</creatorcontrib><creatorcontrib>Contant, S</creatorcontrib><creatorcontrib>Duek, E.A.R</creatorcontrib><creatorcontrib>Marques, F.R</creatorcontrib><creatorcontrib>Wada, M.L.F</creatorcontrib><creatorcontrib>Nunes, S.P</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Polymer (Guilford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zoppi, R.A</au><au>Contant, S</au><au>Duek, E.A.R</au><au>Marques, F.R</au><au>Wada, M.L.F</au><au>Nunes, S.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porous poly( l-lactide) films obtained by immersion precipitation process: morphology, phase separation and culture of VERO cells</atitle><jtitle>Polymer (Guilford)</jtitle><date>1999-06-01</date><risdate>1999</risdate><volume>40</volume><issue>12</issue><spage>3275</spage><epage>3289</epage><pages>3275-3289</pages><issn>0032-3861</issn><eissn>1873-2291</eissn><coden>POLMAG</coden><abstract>In this work, the immersion precipitation process was used to obtain porous poly(
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| subjects | Animal cells Applied sciences Biological and medical sciences Biotechnology Cell culture Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Exact sciences and technology Film morphology Forms of application and semi-finished materials Fundamental and applied biological sciences. Psychology Light scattering Methods. Procedures. Technologies Morphology Phase diagrams Phase separation Poly( l-lactide) Polyesters Polymer industry, paints, wood Porosity Precipitation (chemical) Reaction kinetics Scanning electron microscopy Sheets and films Technology of polymers |
| title | Porous poly( l-lactide) films obtained by immersion precipitation process: morphology, phase separation and culture of VERO cells |
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