The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples
Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involv...
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| Veröffentlicht in: | Phytotherapy research 2019-03, Vol.33 (3), p.591-601 |
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| creator | Mora, Dolly Patricia Pardo Santiago, Karina Basso Conti, Bruno José Oliveira Cardoso, Eliza Conte, Fernanda Lopes Oliveira, Lucas Pires Garcia Assis Golim, Marjorie Uribe, Jaime Fabian Cruz Gutiérrez, Rafael María Buitrago, Mauricio Rey Popova, Milena Trusheva, Boryana Bankova, Vassya García, Orlando Torres Sforcin, José Maurício |
| description | Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs. |
| doi_str_mv | 10.1002/ptr.6246 |
| format | Article |
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Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/ptr.6246</identifier><identifier>PMID: 30488503</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Apoptosis ; Apoptosis - drug effects ; Biocompatibility ; Biomedical materials ; Bone cancer ; Bone Neoplasms - metabolism ; Bone Neoplasms - pathology ; Cell Survival - drug effects ; Cell viability ; Chemical composition ; Colombia ; Cytotoxicity ; Cytotoxins - chemistry ; Cytotoxins - pharmacology ; Diterpenes ; Diterpenes - chemistry ; Diterpenes - pharmacology ; Dog Diseases - metabolism ; Dog Diseases - pathology ; Dogs ; Drug development ; Drugs ; Fatty acids ; Flavonoids ; Flavonoids - chemistry ; Flavonoids - pharmacology ; Flow cytometry ; Free radicals ; Gas Chromatography-Mass Spectrometry ; Invasiveness ; Membrane permeability ; Metastases ; Mitochondria ; mitochondrial membrane potential ; Organic chemistry ; Osteosarcoma ; Osteosarcoma - metabolism ; Osteosarcoma - pathology ; Osteosarcoma cells ; Principal components analysis ; Propolis ; Propolis - chemistry ; Propolis - pharmacology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Rhodamine ; Sarcoma ; Scavenging ; Signal Transduction - drug effects ; Triterpenes ; Triterpenes - chemistry ; Triterpenes - pharmacology ; Tumor Cells, Cultured</subject><ispartof>Phytotherapy research, 2019-03, Vol.33 (3), p.591-601</ispartof><rights>2018 John Wiley & Sons, Ltd.</rights><rights>2019 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3496-2ac9df046ef027381ab2912ca5e90f761d8698325978d3f1179701bf8e0229b53</citedby><cites>FETCH-LOGICAL-c3496-2ac9df046ef027381ab2912ca5e90f761d8698325978d3f1179701bf8e0229b53</cites><orcidid>0000-0003-3466-4895</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fptr.6246$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fptr.6246$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30488503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mora, Dolly Patricia Pardo</creatorcontrib><creatorcontrib>Santiago, Karina Basso</creatorcontrib><creatorcontrib>Conti, Bruno José</creatorcontrib><creatorcontrib>Oliveira Cardoso, Eliza</creatorcontrib><creatorcontrib>Conte, Fernanda Lopes</creatorcontrib><creatorcontrib>Oliveira, Lucas Pires Garcia</creatorcontrib><creatorcontrib>Assis Golim, Marjorie</creatorcontrib><creatorcontrib>Uribe, Jaime Fabian Cruz</creatorcontrib><creatorcontrib>Gutiérrez, Rafael María</creatorcontrib><creatorcontrib>Buitrago, Mauricio Rey</creatorcontrib><creatorcontrib>Popova, Milena</creatorcontrib><creatorcontrib>Trusheva, Boryana</creatorcontrib><creatorcontrib>Bankova, Vassya</creatorcontrib><creatorcontrib>García, Orlando Torres</creatorcontrib><creatorcontrib>Sforcin, José Maurício</creatorcontrib><title>The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples</title><title>Phytotherapy research</title><addtitle>Phytother Res</addtitle><description>Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.</description><subject>Animals</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biocompatibility</subject><subject>Biomedical materials</subject><subject>Bone cancer</subject><subject>Bone Neoplasms - metabolism</subject><subject>Bone Neoplasms - pathology</subject><subject>Cell Survival - drug effects</subject><subject>Cell viability</subject><subject>Chemical composition</subject><subject>Colombia</subject><subject>Cytotoxicity</subject><subject>Cytotoxins - chemistry</subject><subject>Cytotoxins - pharmacology</subject><subject>Diterpenes</subject><subject>Diterpenes - chemistry</subject><subject>Diterpenes - pharmacology</subject><subject>Dog Diseases - metabolism</subject><subject>Dog Diseases - pathology</subject><subject>Dogs</subject><subject>Drug development</subject><subject>Drugs</subject><subject>Fatty acids</subject><subject>Flavonoids</subject><subject>Flavonoids - chemistry</subject><subject>Flavonoids - pharmacology</subject><subject>Flow cytometry</subject><subject>Free radicals</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Invasiveness</subject><subject>Membrane permeability</subject><subject>Metastases</subject><subject>Mitochondria</subject><subject>mitochondrial membrane potential</subject><subject>Organic chemistry</subject><subject>Osteosarcoma</subject><subject>Osteosarcoma - metabolism</subject><subject>Osteosarcoma - pathology</subject><subject>Osteosarcoma cells</subject><subject>Principal components analysis</subject><subject>Propolis</subject><subject>Propolis - chemistry</subject><subject>Propolis - pharmacology</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Rhodamine</subject><subject>Sarcoma</subject><subject>Scavenging</subject><subject>Signal Transduction - drug effects</subject><subject>Triterpenes</subject><subject>Triterpenes - chemistry</subject><subject>Triterpenes - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kV1rFTEQhoNY7LEK_gIJeOPN1nxsdhPvysEvKFjKKXi3ZLMTmrK7WTM5reeH-H_NtlVB8Grm4pmHl3kJecXZKWdMvFtyOm1E3TwhG86Mqbhq5VOyYUbxqub62zF5jnjDGDOC1c_IsWS11orJDfm5uwbqrmEKzo7UxWmJGHKIM7XzQOEW5ow0wWgzDDRHmlf8kGOOP4Kj4D24AkRPlxSXOIayzzRihog2FZ2lDsYR39Oze7lNNodboBYREKdiX2-3cYxTH-xM0U7LCPiCHHk7Irx8nCfk6uOH3fZzdf7105ft2XnlZG2aSlhnBs_qBjwTrdTc9sJw4awCw3zb8EE3RkuhTKsH6TlvTct47zUwIUyv5Al5--At6b_vAXM3BVwD2xniHjvBpVGNVrou6Jt_0Ju4T3NJVyjDWqHKt_8KXYqICXy3pDDZdOg469aqulJVt1ZV0NePwn0_wfAH_N1NAaoH4C6McPivqLvYXd4LfwFmn57a</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Mora, Dolly Patricia Pardo</creator><creator>Santiago, Karina Basso</creator><creator>Conti, Bruno José</creator><creator>Oliveira Cardoso, Eliza</creator><creator>Conte, Fernanda Lopes</creator><creator>Oliveira, Lucas Pires Garcia</creator><creator>Assis Golim, Marjorie</creator><creator>Uribe, Jaime Fabian Cruz</creator><creator>Gutiérrez, Rafael María</creator><creator>Buitrago, Mauricio Rey</creator><creator>Popova, Milena</creator><creator>Trusheva, Boryana</creator><creator>Bankova, Vassya</creator><creator>García, Orlando Torres</creator><creator>Sforcin, José Maurício</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3466-4895</orcidid></search><sort><creationdate>201903</creationdate><title>The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples</title><author>Mora, Dolly Patricia Pardo ; Santiago, Karina Basso ; Conti, Bruno José ; Oliveira Cardoso, Eliza ; Conte, Fernanda Lopes ; Oliveira, Lucas Pires Garcia ; Assis Golim, Marjorie ; Uribe, Jaime Fabian Cruz ; Gutiérrez, Rafael María ; Buitrago, Mauricio Rey ; Popova, Milena ; Trusheva, Boryana ; Bankova, Vassya ; García, Orlando Torres ; Sforcin, José Maurício</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3496-2ac9df046ef027381ab2912ca5e90f761d8698325978d3f1179701bf8e0229b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antioxidants - 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metabolism</topic><topic>Osteosarcoma - pathology</topic><topic>Osteosarcoma cells</topic><topic>Principal components analysis</topic><topic>Propolis</topic><topic>Propolis - chemistry</topic><topic>Propolis - pharmacology</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Rhodamine</topic><topic>Sarcoma</topic><topic>Scavenging</topic><topic>Signal Transduction - drug effects</topic><topic>Triterpenes</topic><topic>Triterpenes - chemistry</topic><topic>Triterpenes - pharmacology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mora, Dolly Patricia Pardo</creatorcontrib><creatorcontrib>Santiago, Karina Basso</creatorcontrib><creatorcontrib>Conti, Bruno José</creatorcontrib><creatorcontrib>Oliveira Cardoso, Eliza</creatorcontrib><creatorcontrib>Conte, Fernanda Lopes</creatorcontrib><creatorcontrib>Oliveira, Lucas Pires Garcia</creatorcontrib><creatorcontrib>Assis Golim, Marjorie</creatorcontrib><creatorcontrib>Uribe, Jaime Fabian Cruz</creatorcontrib><creatorcontrib>Gutiérrez, Rafael María</creatorcontrib><creatorcontrib>Buitrago, Mauricio Rey</creatorcontrib><creatorcontrib>Popova, Milena</creatorcontrib><creatorcontrib>Trusheva, Boryana</creatorcontrib><creatorcontrib>Bankova, Vassya</creatorcontrib><creatorcontrib>García, Orlando Torres</creatorcontrib><creatorcontrib>Sforcin, José Maurício</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mora, Dolly Patricia Pardo</au><au>Santiago, Karina Basso</au><au>Conti, Bruno José</au><au>Oliveira Cardoso, Eliza</au><au>Conte, Fernanda Lopes</au><au>Oliveira, Lucas Pires Garcia</au><au>Assis Golim, Marjorie</au><au>Uribe, Jaime Fabian Cruz</au><au>Gutiérrez, Rafael María</au><au>Buitrago, Mauricio Rey</au><au>Popova, Milena</au><au>Trusheva, Boryana</au><au>Bankova, Vassya</au><au>García, Orlando Torres</au><au>Sforcin, José Maurício</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother Res</addtitle><date>2019-03</date><risdate>2019</risdate><volume>33</volume><issue>3</issue><spage>591</spage><epage>601</epage><pages>591-601</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>Osteosarcoma (OSA) is a type of bone cancer showing an aggressive biological behavior with metastatic progression. Because propolis potential for the development of new antitumoral drugs has been indicated, we evaluated the chemical composition of Colombian propolis samples and the mechanisms involved in their cytotoxic effects on OSA cells. The chemical composition was analyzed by GC–MS and the DPPH free radical scavenging activity was measured. Cluster and principal components analysis were used to establish an association with their inhibitory concentration 50% (IC50). Cell viability was analyzed by MTT assay; apoptosis was determined by flow cytometry; mitochondrial membrane permeability and reactive oxygen species were evaluated by rhodamine 123 and DCFH‐DA. Transwell assay was used to evaluate the invasiveness of propolis‐treated cells. Samples were grouped: Cluster 1 contained diterpenes and benzophenones and showed the highest antiradical activity; Cluster 2 was characterized by triterpenes, fatty acid, and diterpenes. Usm contained diterpenes and triterpenes different of the other samples and Sil contained triterpenes and flavonoids. Apoptosis, mitochondrial membrane alteration, and suppression of cell invasion were the main mechanisms involved in the inhibition of OSA cells in vitro, suggesting the potential of Colombian propolis to discover new antitumor drugs.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30488503</pmid><doi>10.1002/ptr.6246</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3466-4895</orcidid></addata></record> |
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| subjects | Animals Antioxidants - chemistry Antioxidants - pharmacology Apoptosis Apoptosis - drug effects Biocompatibility Biomedical materials Bone cancer Bone Neoplasms - metabolism Bone Neoplasms - pathology Cell Survival - drug effects Cell viability Chemical composition Colombia Cytotoxicity Cytotoxins - chemistry Cytotoxins - pharmacology Diterpenes Diterpenes - chemistry Diterpenes - pharmacology Dog Diseases - metabolism Dog Diseases - pathology Dogs Drug development Drugs Fatty acids Flavonoids Flavonoids - chemistry Flavonoids - pharmacology Flow cytometry Free radicals Gas Chromatography-Mass Spectrometry Invasiveness Membrane permeability Metastases Mitochondria mitochondrial membrane potential Organic chemistry Osteosarcoma Osteosarcoma - metabolism Osteosarcoma - pathology Osteosarcoma cells Principal components analysis Propolis Propolis - chemistry Propolis - pharmacology Reactive oxygen species Reactive Oxygen Species - metabolism Rhodamine Sarcoma Scavenging Signal Transduction - drug effects Triterpenes Triterpenes - chemistry Triterpenes - pharmacology Tumor Cells, Cultured |
| title | The chemical composition and events related to the cytotoxic effects of propolis on osteosarcoma cells: A comparative assessment of Colombian samples |
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