Neuropharmacological effects of essential oil from the leaves of Croton conduplicatus Kunth and possible mechanisms of action involved
Croton conduplicatus Kunth (Euphorbiaceae) is a Brazilian aromatic medicinal plant, widely known as “quebra-faca”. In folk medicine, its leaves and stem-barks are used as a natural analgesic for the treatment of headaches. In this study, we describe for the first time the neuropharmacological potent...
Gespeichert in:
| Veröffentlicht in: | Journal of ethnopharmacology 2018-07, Vol.221 (NA), p.65-76 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 76 |
|---|---|
| container_issue | NA |
| container_start_page | 65 |
| container_title | Journal of ethnopharmacology |
| container_volume | 221 |
| creator | Oliveira Júnior, Raimundo Gonçalves de Ferraz, Christiane Adrielly Alves Silva, Juliane Cabral de Andrade Teles, Roxana Braga Silva, Mariana Gama Diniz, Tâmara Coimbra dos Santos, Uiliane Soares de Souza, Ana Valéria Vieira Nunes, Carlos Eduardo Pereira Salvador, Marcos José Lorenzo, Vitor Prates Quintans Júnior, Lucindo José Almeida, Jackson Roberto Guedes da Silva |
| description | Croton conduplicatus Kunth (Euphorbiaceae) is a Brazilian aromatic medicinal plant, widely known as “quebra-faca”. In folk medicine, its leaves and stem-barks are used as a natural analgesic for the treatment of headaches.
In this study, we describe for the first time the neuropharmacological potential of the essential oil obtained from the leaves of Croton conduplicatus (EO) in experimental models of pain, anxiety and insomnia. The mechanisms of action involved in these activities were also investigated.
Different experimental models were used to evaluate the antinociceptive (acetic acid, formalin-induced nociception and hot plate tests), anxiolytic (elevated plus maze and hole board tests) and sedative (thiopental-induced sleeping time) effects of EO in mice. EO was evaluated in three different doses (25, 50 and 100 mg/kg, i.p.) and compared with positive and negative controls in all experimental protocols. When appropriate, animals were pretreated with pharmacological antagonists (naloxone, atropine and flumazenil) in order to evaluate the mechanisms of action involved. A docking study also was performed to identify possible targets involved.
EO (25, 50 and 100 mg/kg, i.p.) demonstrated a significant antinociceptive activity in all experimental models. Pretreatment with naloxone or atropine reversed the antinociceptive response (p |
| doi_str_mv | 10.1016/j.jep.2018.04.009 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2138567985</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378874118300382</els_id><sourcerecordid>2138567985</sourcerecordid><originalsourceid>FETCH-LOGICAL-c495t-2b946172c323748aa9ac2e5dfd58284ed97f74b79585b73bcfb69d093f613a0d3</originalsourceid><addsrcrecordid>eNqFkcuOFCEYRonROO3oA7gxLN1Uy60KiCvT8RYnutE1oeDHpkMVJVR14gv43DLTo0tdkZBzvgQOQs8p2VNCh1en_QmWPSNU7YnYE6IfoB1VknWyl_wh2hEuVaekoFfoSa0nQoikgjxGV0wPTDItd-jXZ9hKXo62TNbllL9HZxOGEMCtFeeAoVaY19guc0w4lDzh9Qg4gT3DHXAoec0zdnn225Kavm4Vf9rm9Yjt7PGSa41jAjyBO9o51unOsm6NzYrzOacz-KfoUbCpwrP78xp9e_f26-FDd_Pl_cfDm5vOCd2vHRu1GKhkjjMuhbJWW8eg98H3iikBXssgxSh1r_pR8tGFcdCeaB4Gyi3x_Bq9vOwuJf_YoK5mitVBSnaGvFXDKFf9ILXq_48SxgWRA-cNpRfUlfbaAsEsJU62_DSUmNtS5mRaKXNbyhBhWqnmvLif38YJ_F_jT5oGvL4A0P7jHKGY6iLMDnwsLY7xOf5j_jfEwqZH</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2023407633</pqid></control><display><type>article</type><title>Neuropharmacological effects of essential oil from the leaves of Croton conduplicatus Kunth and possible mechanisms of action involved</title><source>Elsevier ScienceDirect Journals</source><creator>Oliveira Júnior, Raimundo Gonçalves de ; Ferraz, Christiane Adrielly Alves ; Silva, Juliane Cabral ; de Andrade Teles, Roxana Braga ; Silva, Mariana Gama ; Diniz, Tâmara Coimbra ; dos Santos, Uiliane Soares ; de Souza, Ana Valéria Vieira ; Nunes, Carlos Eduardo Pereira ; Salvador, Marcos José ; Lorenzo, Vitor Prates ; Quintans Júnior, Lucindo José ; Almeida, Jackson Roberto Guedes da Silva</creator><creatorcontrib>Oliveira Júnior, Raimundo Gonçalves de ; Ferraz, Christiane Adrielly Alves ; Silva, Juliane Cabral ; de Andrade Teles, Roxana Braga ; Silva, Mariana Gama ; Diniz, Tâmara Coimbra ; dos Santos, Uiliane Soares ; de Souza, Ana Valéria Vieira ; Nunes, Carlos Eduardo Pereira ; Salvador, Marcos José ; Lorenzo, Vitor Prates ; Quintans Júnior, Lucindo José ; Almeida, Jackson Roberto Guedes da Silva</creatorcontrib><description>Croton conduplicatus Kunth (Euphorbiaceae) is a Brazilian aromatic medicinal plant, widely known as “quebra-faca”. In folk medicine, its leaves and stem-barks are used as a natural analgesic for the treatment of headaches.
In this study, we describe for the first time the neuropharmacological potential of the essential oil obtained from the leaves of Croton conduplicatus (EO) in experimental models of pain, anxiety and insomnia. The mechanisms of action involved in these activities were also investigated.
Different experimental models were used to evaluate the antinociceptive (acetic acid, formalin-induced nociception and hot plate tests), anxiolytic (elevated plus maze and hole board tests) and sedative (thiopental-induced sleeping time) effects of EO in mice. EO was evaluated in three different doses (25, 50 and 100 mg/kg, i.p.) and compared with positive and negative controls in all experimental protocols. When appropriate, animals were pretreated with pharmacological antagonists (naloxone, atropine and flumazenil) in order to evaluate the mechanisms of action involved. A docking study also was performed to identify possible targets involved.
EO (25, 50 and 100 mg/kg, i.p.) demonstrated a significant antinociceptive activity in all experimental models. Pretreatment with naloxone or atropine reversed the antinociceptive response (p < 0.05), suggesting the involvement of opioid and muscarinic receptors, respectively. A docking study was performed with the major components identified in EO (1,8 cineole − 21.42%, spathulenol – 15.47%, p-cymene – 12.41% and caryophyllene oxide – 12.15%), demonstrating favorable interaction profile with different subtypes of muscarinic (M2, M3 and M4) and opioids (delta and mu) receptors. EO also showed anxiolytic (mainly at doses of 25 and 50 mg/kg, i.p.) and sedative (only at the dose of 100 mg/kg, i.p.) effects in mice. These pharmacological responses were reversed by flumazenil (p < 0.05), indicating possible involvement of GABAA receptors.
Our findings support the traditional use of this plant as a natural analgesic and suggest that EO is a multi-target natural product, presenting not only antinociceptive effect but also anxiolytic and sedative activities depending on the dose used.
[Display omitted]</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2018.04.009</identifier><identifier>PMID: 29627297</identifier><language>eng</language><publisher>Ireland: Elsevier B.V</publisher><subject>Anxiety ; Essential oil ; Insomnia ; Medicinal plants ; Pain</subject><ispartof>Journal of ethnopharmacology, 2018-07, Vol.221 (NA), p.65-76</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-2b946172c323748aa9ac2e5dfd58284ed97f74b79585b73bcfb69d093f613a0d3</citedby><cites>FETCH-LOGICAL-c495t-2b946172c323748aa9ac2e5dfd58284ed97f74b79585b73bcfb69d093f613a0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378874118300382$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29627297$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira Júnior, Raimundo Gonçalves de</creatorcontrib><creatorcontrib>Ferraz, Christiane Adrielly Alves</creatorcontrib><creatorcontrib>Silva, Juliane Cabral</creatorcontrib><creatorcontrib>de Andrade Teles, Roxana Braga</creatorcontrib><creatorcontrib>Silva, Mariana Gama</creatorcontrib><creatorcontrib>Diniz, Tâmara Coimbra</creatorcontrib><creatorcontrib>dos Santos, Uiliane Soares</creatorcontrib><creatorcontrib>de Souza, Ana Valéria Vieira</creatorcontrib><creatorcontrib>Nunes, Carlos Eduardo Pereira</creatorcontrib><creatorcontrib>Salvador, Marcos José</creatorcontrib><creatorcontrib>Lorenzo, Vitor Prates</creatorcontrib><creatorcontrib>Quintans Júnior, Lucindo José</creatorcontrib><creatorcontrib>Almeida, Jackson Roberto Guedes da Silva</creatorcontrib><title>Neuropharmacological effects of essential oil from the leaves of Croton conduplicatus Kunth and possible mechanisms of action involved</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Croton conduplicatus Kunth (Euphorbiaceae) is a Brazilian aromatic medicinal plant, widely known as “quebra-faca”. In folk medicine, its leaves and stem-barks are used as a natural analgesic for the treatment of headaches.
In this study, we describe for the first time the neuropharmacological potential of the essential oil obtained from the leaves of Croton conduplicatus (EO) in experimental models of pain, anxiety and insomnia. The mechanisms of action involved in these activities were also investigated.
Different experimental models were used to evaluate the antinociceptive (acetic acid, formalin-induced nociception and hot plate tests), anxiolytic (elevated plus maze and hole board tests) and sedative (thiopental-induced sleeping time) effects of EO in mice. EO was evaluated in three different doses (25, 50 and 100 mg/kg, i.p.) and compared with positive and negative controls in all experimental protocols. When appropriate, animals were pretreated with pharmacological antagonists (naloxone, atropine and flumazenil) in order to evaluate the mechanisms of action involved. A docking study also was performed to identify possible targets involved.
EO (25, 50 and 100 mg/kg, i.p.) demonstrated a significant antinociceptive activity in all experimental models. Pretreatment with naloxone or atropine reversed the antinociceptive response (p < 0.05), suggesting the involvement of opioid and muscarinic receptors, respectively. A docking study was performed with the major components identified in EO (1,8 cineole − 21.42%, spathulenol – 15.47%, p-cymene – 12.41% and caryophyllene oxide – 12.15%), demonstrating favorable interaction profile with different subtypes of muscarinic (M2, M3 and M4) and opioids (delta and mu) receptors. EO also showed anxiolytic (mainly at doses of 25 and 50 mg/kg, i.p.) and sedative (only at the dose of 100 mg/kg, i.p.) effects in mice. These pharmacological responses were reversed by flumazenil (p < 0.05), indicating possible involvement of GABAA receptors.
Our findings support the traditional use of this plant as a natural analgesic and suggest that EO is a multi-target natural product, presenting not only antinociceptive effect but also anxiolytic and sedative activities depending on the dose used.
[Display omitted]</description><subject>Anxiety</subject><subject>Essential oil</subject><subject>Insomnia</subject><subject>Medicinal plants</subject><subject>Pain</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqFkcuOFCEYRonROO3oA7gxLN1Uy60KiCvT8RYnutE1oeDHpkMVJVR14gv43DLTo0tdkZBzvgQOQs8p2VNCh1en_QmWPSNU7YnYE6IfoB1VknWyl_wh2hEuVaekoFfoSa0nQoikgjxGV0wPTDItd-jXZ9hKXo62TNbllL9HZxOGEMCtFeeAoVaY19guc0w4lDzh9Qg4gT3DHXAoec0zdnn225Kavm4Vf9rm9Yjt7PGSa41jAjyBO9o51unOsm6NzYrzOacz-KfoUbCpwrP78xp9e_f26-FDd_Pl_cfDm5vOCd2vHRu1GKhkjjMuhbJWW8eg98H3iikBXssgxSh1r_pR8tGFcdCeaB4Gyi3x_Bq9vOwuJf_YoK5mitVBSnaGvFXDKFf9ILXq_48SxgWRA-cNpRfUlfbaAsEsJU62_DSUmNtS5mRaKXNbyhBhWqnmvLif38YJ_F_jT5oGvL4A0P7jHKGY6iLMDnwsLY7xOf5j_jfEwqZH</recordid><startdate>20180715</startdate><enddate>20180715</enddate><creator>Oliveira Júnior, Raimundo Gonçalves de</creator><creator>Ferraz, Christiane Adrielly Alves</creator><creator>Silva, Juliane Cabral</creator><creator>de Andrade Teles, Roxana Braga</creator><creator>Silva, Mariana Gama</creator><creator>Diniz, Tâmara Coimbra</creator><creator>dos Santos, Uiliane Soares</creator><creator>de Souza, Ana Valéria Vieira</creator><creator>Nunes, Carlos Eduardo Pereira</creator><creator>Salvador, Marcos José</creator><creator>Lorenzo, Vitor Prates</creator><creator>Quintans Júnior, Lucindo José</creator><creator>Almeida, Jackson Roberto Guedes da Silva</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180715</creationdate><title>Neuropharmacological effects of essential oil from the leaves of Croton conduplicatus Kunth and possible mechanisms of action involved</title><author>Oliveira Júnior, Raimundo Gonçalves de ; Ferraz, Christiane Adrielly Alves ; Silva, Juliane Cabral ; de Andrade Teles, Roxana Braga ; Silva, Mariana Gama ; Diniz, Tâmara Coimbra ; dos Santos, Uiliane Soares ; de Souza, Ana Valéria Vieira ; Nunes, Carlos Eduardo Pereira ; Salvador, Marcos José ; Lorenzo, Vitor Prates ; Quintans Júnior, Lucindo José ; Almeida, Jackson Roberto Guedes da Silva</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-2b946172c323748aa9ac2e5dfd58284ed97f74b79585b73bcfb69d093f613a0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anxiety</topic><topic>Essential oil</topic><topic>Insomnia</topic><topic>Medicinal plants</topic><topic>Pain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira Júnior, Raimundo Gonçalves de</creatorcontrib><creatorcontrib>Ferraz, Christiane Adrielly Alves</creatorcontrib><creatorcontrib>Silva, Juliane Cabral</creatorcontrib><creatorcontrib>de Andrade Teles, Roxana Braga</creatorcontrib><creatorcontrib>Silva, Mariana Gama</creatorcontrib><creatorcontrib>Diniz, Tâmara Coimbra</creatorcontrib><creatorcontrib>dos Santos, Uiliane Soares</creatorcontrib><creatorcontrib>de Souza, Ana Valéria Vieira</creatorcontrib><creatorcontrib>Nunes, Carlos Eduardo Pereira</creatorcontrib><creatorcontrib>Salvador, Marcos José</creatorcontrib><creatorcontrib>Lorenzo, Vitor Prates</creatorcontrib><creatorcontrib>Quintans Júnior, Lucindo José</creatorcontrib><creatorcontrib>Almeida, Jackson Roberto Guedes da Silva</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira Júnior, Raimundo Gonçalves de</au><au>Ferraz, Christiane Adrielly Alves</au><au>Silva, Juliane Cabral</au><au>de Andrade Teles, Roxana Braga</au><au>Silva, Mariana Gama</au><au>Diniz, Tâmara Coimbra</au><au>dos Santos, Uiliane Soares</au><au>de Souza, Ana Valéria Vieira</au><au>Nunes, Carlos Eduardo Pereira</au><au>Salvador, Marcos José</au><au>Lorenzo, Vitor Prates</au><au>Quintans Júnior, Lucindo José</au><au>Almeida, Jackson Roberto Guedes da Silva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuropharmacological effects of essential oil from the leaves of Croton conduplicatus Kunth and possible mechanisms of action involved</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2018-07-15</date><risdate>2018</risdate><volume>221</volume><issue>NA</issue><spage>65</spage><epage>76</epage><pages>65-76</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><abstract>Croton conduplicatus Kunth (Euphorbiaceae) is a Brazilian aromatic medicinal plant, widely known as “quebra-faca”. In folk medicine, its leaves and stem-barks are used as a natural analgesic for the treatment of headaches.
In this study, we describe for the first time the neuropharmacological potential of the essential oil obtained from the leaves of Croton conduplicatus (EO) in experimental models of pain, anxiety and insomnia. The mechanisms of action involved in these activities were also investigated.
Different experimental models were used to evaluate the antinociceptive (acetic acid, formalin-induced nociception and hot plate tests), anxiolytic (elevated plus maze and hole board tests) and sedative (thiopental-induced sleeping time) effects of EO in mice. EO was evaluated in three different doses (25, 50 and 100 mg/kg, i.p.) and compared with positive and negative controls in all experimental protocols. When appropriate, animals were pretreated with pharmacological antagonists (naloxone, atropine and flumazenil) in order to evaluate the mechanisms of action involved. A docking study also was performed to identify possible targets involved.
EO (25, 50 and 100 mg/kg, i.p.) demonstrated a significant antinociceptive activity in all experimental models. Pretreatment with naloxone or atropine reversed the antinociceptive response (p < 0.05), suggesting the involvement of opioid and muscarinic receptors, respectively. A docking study was performed with the major components identified in EO (1,8 cineole − 21.42%, spathulenol – 15.47%, p-cymene – 12.41% and caryophyllene oxide – 12.15%), demonstrating favorable interaction profile with different subtypes of muscarinic (M2, M3 and M4) and opioids (delta and mu) receptors. EO also showed anxiolytic (mainly at doses of 25 and 50 mg/kg, i.p.) and sedative (only at the dose of 100 mg/kg, i.p.) effects in mice. These pharmacological responses were reversed by flumazenil (p < 0.05), indicating possible involvement of GABAA receptors.
Our findings support the traditional use of this plant as a natural analgesic and suggest that EO is a multi-target natural product, presenting not only antinociceptive effect but also anxiolytic and sedative activities depending on the dose used.
[Display omitted]</abstract><cop>Ireland</cop><pub>Elsevier B.V</pub><pmid>29627297</pmid><doi>10.1016/j.jep.2018.04.009</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-8741 |
| ispartof | Journal of ethnopharmacology, 2018-07, Vol.221 (NA), p.65-76 |
| issn | 0378-8741 1872-7573 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2138567985 |
| source | Elsevier ScienceDirect Journals |
| subjects | Anxiety Essential oil Insomnia Medicinal plants Pain |
| title | Neuropharmacological effects of essential oil from the leaves of Croton conduplicatus Kunth and possible mechanisms of action involved |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T21%3A35%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neuropharmacological%20effects%20of%20essential%20oil%20from%20the%20leaves%20of%20Croton%20conduplicatus%20Kunth%20and%20possible%20mechanisms%20of%20action%20involved&rft.jtitle=Journal%20of%20ethnopharmacology&rft.au=Oliveira%20J%C3%BAnior,%20Raimundo%20Gon%C3%A7alves%20de&rft.date=2018-07-15&rft.volume=221&rft.issue=NA&rft.spage=65&rft.epage=76&rft.pages=65-76&rft.issn=0378-8741&rft.eissn=1872-7573&rft_id=info:doi/10.1016/j.jep.2018.04.009&rft_dat=%3Cproquest_cross%3E2138567985%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2023407633&rft_id=info:pmid/29627297&rft_els_id=S0378874118300382&rfr_iscdi=true |