Biofilm dynamics studied with microsensors and molecular techniques
Here we present preliminary data on the development of a biofilm from a wastewater treatment plant studied with microsensors and molecular techniques. The development during biofilm growth of oxygen, sulfide and pH profiles was measured with microsensors. Anoxic zones developed within one week and f...
Gespeichert in:
| Veröffentlicht in: | Water science and technology 1998, Vol.37 (4-5), p.125-129 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 129 |
|---|---|
| container_issue | 4-5 |
| container_start_page | 125 |
| container_title | Water science and technology |
| container_volume | 37 |
| creator | Santegoeds, Cecilia M. Muyzer, Gerard de Beer, Dirk |
| description | Here we present preliminary data on the development of a biofilm from a wastewater treatment plant studied with microsensors and molecular techniques. The development during biofilm growth of oxygen, sulfide and pH profiles was measured with microsensors. Anoxic zones developed within one week and further increased during the following weeks. However, sulfide production was delayed and was first detected in a six-week-old biofilm. With denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 165 rRNA gene fragments the sequence of the bacterial community was followed showing an increasing complexity of the biofilm community during development. In a mature biofilm the influence of nitrate on sulfide production was studied by measuring oxygen, sulfide, pH, nitrite and nitrate profiles with microsensors. Sulfide production was detected deeper in the biofilm and in lower concentrations, when nitrate was added to the medium. The DGGE pattern of the mature biofilm showed both differences and similarities with the DGGE pattern of the 12-week-old biofilm. In particular the RNA pattern changed when nitrate was added to the medium, indicating a change in activity of certain strains. |
| doi_str_mv | 10.1016/S0273-1223(98)00094-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21371406</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0273122398000948</els_id><sourcerecordid>13669428</sourcerecordid><originalsourceid>FETCH-LOGICAL-c343t-d0ed1d37d9a11d7aad12028606cde39f31d07981a86c437173cf77854ab750da3</originalsourceid><addsrcrecordid>eNqNkUtLxDAUhYMoOI7-BKEgiC6quU2ax0p08AUDLtR1iEnKZGibMWmV-fe2M-LCja4uXL5zLvcchI4BXwAGdvmMC05yKApyJsU5xljSXOygCUjJcslJsYsmP8g-OkhpOUCcUDxBsxsfKl83mV23uvEmZanrrXc2-_TdIhs2MSTXphBTplubNaF2pq91zDpnFq1_7106RHuVrpM7-p5T9Hp3-zJ7yOdP94-z63luCCVdbrGzYAm3UgNYrrWFAheCYWasI7IiYDGXArRghhIOnJiKc1FS_cZLbDWZotOt7yqG8W6nGp-Mq2vdutAnVcCgopj9CQIrKWD6D5AwJmkhBvDkF7gMfWyHbxVISgiAKOVAlVtqDC1FV6lV9I2OawVYjVWpTVVq7EFJoTZVqdH9aqtzQ3of3kWVjHetcdZHZzplg__D4Qu3lpnh</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1943311859</pqid></control><display><type>article</type><title>Biofilm dynamics studied with microsensors and molecular techniques</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Santegoeds, Cecilia M. ; Muyzer, Gerard ; de Beer, Dirk</creator><contributor>The Conference Program Committee</contributor><creatorcontrib>Santegoeds, Cecilia M. ; Muyzer, Gerard ; de Beer, Dirk ; The Conference Program Committee</creatorcontrib><description>Here we present preliminary data on the development of a biofilm from a wastewater treatment plant studied with microsensors and molecular techniques. The development during biofilm growth of oxygen, sulfide and pH profiles was measured with microsensors. Anoxic zones developed within one week and further increased during the following weeks. However, sulfide production was delayed and was first detected in a six-week-old biofilm. With denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 165 rRNA gene fragments the sequence of the bacterial community was followed showing an increasing complexity of the biofilm community during development. In a mature biofilm the influence of nitrate on sulfide production was studied by measuring oxygen, sulfide, pH, nitrite and nitrate profiles with microsensors. Sulfide production was detected deeper in the biofilm and in lower concentrations, when nitrate was added to the medium. The DGGE pattern of the mature biofilm showed both differences and similarities with the DGGE pattern of the 12-week-old biofilm. In particular the RNA pattern changed when nitrate was added to the medium, indicating a change in activity of certain strains.</description><identifier>ISSN: 0273-1223</identifier><identifier>EISSN: 1996-9732</identifier><identifier>DOI: 10.1016/S0273-1223(98)00094-8</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Activated sludge ; Activated sludge process ; Analytical methods ; Anoxia ; Bacteria ; bacterial biofilm ; Biofilms ; Communities ; Denitrification ; DGGE ; DNA ; Dynamics ; Fragments ; Gel electrophoresis ; Gels ; Growth kinetics ; Microbiological strains ; Microsensors ; Nitrates ; Nucleic acids ; Nucleotide sequence ; Oxygen ; Oxygen sensors ; PCR ; pH effects ; pH sensors ; population dynamics ; Profiles ; Ribonucleic acid ; RNA ; rRNA 16S ; Sewage treatment plants ; sulfate reduction ; Sulfides ; Sulphides ; Wastewater ; Wastewater treatment ; Wastewater treatment plants</subject><ispartof>Water science and technology, 1998, Vol.37 (4-5), p.125-129</ispartof><rights>1998 International Association on Water Quality</rights><rights>Copyright IWA Publishing Feb 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-d0ed1d37d9a11d7aad12028606cde39f31d07981a86c437173cf77854ab750da3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,778,782,787,788,4012,4038,4039,23913,23914,25123,27906,27907,27908</link.rule.ids></links><search><contributor>The Conference Program Committee</contributor><creatorcontrib>Santegoeds, Cecilia M.</creatorcontrib><creatorcontrib>Muyzer, Gerard</creatorcontrib><creatorcontrib>de Beer, Dirk</creatorcontrib><title>Biofilm dynamics studied with microsensors and molecular techniques</title><title>Water science and technology</title><description>Here we present preliminary data on the development of a biofilm from a wastewater treatment plant studied with microsensors and molecular techniques. The development during biofilm growth of oxygen, sulfide and pH profiles was measured with microsensors. Anoxic zones developed within one week and further increased during the following weeks. However, sulfide production was delayed and was first detected in a six-week-old biofilm. With denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 165 rRNA gene fragments the sequence of the bacterial community was followed showing an increasing complexity of the biofilm community during development. In a mature biofilm the influence of nitrate on sulfide production was studied by measuring oxygen, sulfide, pH, nitrite and nitrate profiles with microsensors. Sulfide production was detected deeper in the biofilm and in lower concentrations, when nitrate was added to the medium. The DGGE pattern of the mature biofilm showed both differences and similarities with the DGGE pattern of the 12-week-old biofilm. In particular the RNA pattern changed when nitrate was added to the medium, indicating a change in activity of certain strains.</description><subject>Activated sludge</subject><subject>Activated sludge process</subject><subject>Analytical methods</subject><subject>Anoxia</subject><subject>Bacteria</subject><subject>bacterial biofilm</subject><subject>Biofilms</subject><subject>Communities</subject><subject>Denitrification</subject><subject>DGGE</subject><subject>DNA</subject><subject>Dynamics</subject><subject>Fragments</subject><subject>Gel electrophoresis</subject><subject>Gels</subject><subject>Growth kinetics</subject><subject>Microbiological strains</subject><subject>Microsensors</subject><subject>Nitrates</subject><subject>Nucleic acids</subject><subject>Nucleotide sequence</subject><subject>Oxygen</subject><subject>Oxygen sensors</subject><subject>PCR</subject><subject>pH effects</subject><subject>pH sensors</subject><subject>population dynamics</subject><subject>Profiles</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>rRNA 16S</subject><subject>Sewage treatment plants</subject><subject>sulfate reduction</subject><subject>Sulfides</subject><subject>Sulphides</subject><subject>Wastewater</subject><subject>Wastewater treatment</subject><subject>Wastewater treatment plants</subject><issn>0273-1223</issn><issn>1996-9732</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqNkUtLxDAUhYMoOI7-BKEgiC6quU2ax0p08AUDLtR1iEnKZGibMWmV-fe2M-LCja4uXL5zLvcchI4BXwAGdvmMC05yKApyJsU5xljSXOygCUjJcslJsYsmP8g-OkhpOUCcUDxBsxsfKl83mV23uvEmZanrrXc2-_TdIhs2MSTXphBTplubNaF2pq91zDpnFq1_7106RHuVrpM7-p5T9Hp3-zJ7yOdP94-z63luCCVdbrGzYAm3UgNYrrWFAheCYWasI7IiYDGXArRghhIOnJiKc1FS_cZLbDWZotOt7yqG8W6nGp-Mq2vdutAnVcCgopj9CQIrKWD6D5AwJmkhBvDkF7gMfWyHbxVISgiAKOVAlVtqDC1FV6lV9I2OawVYjVWpTVVq7EFJoTZVqdH9aqtzQ3of3kWVjHetcdZHZzplg__D4Qu3lpnh</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>Santegoeds, Cecilia M.</creator><creator>Muyzer, Gerard</creator><creator>de Beer, Dirk</creator><general>Elsevier Ltd</general><general>IWA Publishing</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QH</scope><scope>7UA</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H96</scope><scope>H97</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>L6V</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>7TV</scope></search><sort><creationdate>1998</creationdate><title>Biofilm dynamics studied with microsensors and molecular techniques</title><author>Santegoeds, Cecilia M. ; Muyzer, Gerard ; de Beer, Dirk</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-d0ed1d37d9a11d7aad12028606cde39f31d07981a86c437173cf77854ab750da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Activated sludge</topic><topic>Activated sludge process</topic><topic>Analytical methods</topic><topic>Anoxia</topic><topic>Bacteria</topic><topic>bacterial biofilm</topic><topic>Biofilms</topic><topic>Communities</topic><topic>Denitrification</topic><topic>DGGE</topic><topic>DNA</topic><topic>Dynamics</topic><topic>Fragments</topic><topic>Gel electrophoresis</topic><topic>Gels</topic><topic>Growth kinetics</topic><topic>Microbiological strains</topic><topic>Microsensors</topic><topic>Nitrates</topic><topic>Nucleic acids</topic><topic>Nucleotide sequence</topic><topic>Oxygen</topic><topic>Oxygen sensors</topic><topic>PCR</topic><topic>pH effects</topic><topic>pH sensors</topic><topic>population dynamics</topic><topic>Profiles</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>rRNA 16S</topic><topic>Sewage treatment plants</topic><topic>sulfate reduction</topic><topic>Sulfides</topic><topic>Sulphides</topic><topic>Wastewater</topic><topic>Wastewater treatment</topic><topic>Wastewater treatment plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santegoeds, Cecilia M.</creatorcontrib><creatorcontrib>Muyzer, Gerard</creatorcontrib><creatorcontrib>de Beer, Dirk</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Water Resources Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>Pollution Abstracts</collection><jtitle>Water science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santegoeds, Cecilia M.</au><au>Muyzer, Gerard</au><au>de Beer, Dirk</au><au>The Conference Program Committee</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biofilm dynamics studied with microsensors and molecular techniques</atitle><jtitle>Water science and technology</jtitle><date>1998</date><risdate>1998</risdate><volume>37</volume><issue>4-5</issue><spage>125</spage><epage>129</epage><pages>125-129</pages><issn>0273-1223</issn><eissn>1996-9732</eissn><abstract>Here we present preliminary data on the development of a biofilm from a wastewater treatment plant studied with microsensors and molecular techniques. The development during biofilm growth of oxygen, sulfide and pH profiles was measured with microsensors. Anoxic zones developed within one week and further increased during the following weeks. However, sulfide production was delayed and was first detected in a six-week-old biofilm. With denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 165 rRNA gene fragments the sequence of the bacterial community was followed showing an increasing complexity of the biofilm community during development. In a mature biofilm the influence of nitrate on sulfide production was studied by measuring oxygen, sulfide, pH, nitrite and nitrate profiles with microsensors. Sulfide production was detected deeper in the biofilm and in lower concentrations, when nitrate was added to the medium. The DGGE pattern of the mature biofilm showed both differences and similarities with the DGGE pattern of the 12-week-old biofilm. In particular the RNA pattern changed when nitrate was added to the medium, indicating a change in activity of certain strains.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0273-1223(98)00094-8</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-1223 |
| ispartof | Water science and technology, 1998, Vol.37 (4-5), p.125-129 |
| issn | 0273-1223 1996-9732 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21371406 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Activated sludge Activated sludge process Analytical methods Anoxia Bacteria bacterial biofilm Biofilms Communities Denitrification DGGE DNA Dynamics Fragments Gel electrophoresis Gels Growth kinetics Microbiological strains Microsensors Nitrates Nucleic acids Nucleotide sequence Oxygen Oxygen sensors PCR pH effects pH sensors population dynamics Profiles Ribonucleic acid RNA rRNA 16S Sewage treatment plants sulfate reduction Sulfides Sulphides Wastewater Wastewater treatment Wastewater treatment plants |
| title | Biofilm dynamics studied with microsensors and molecular techniques |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T18%3A42%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Biofilm%20dynamics%20studied%20with%20microsensors%20and%20molecular%20techniques&rft.jtitle=Water%20science%20and%20technology&rft.au=Santegoeds,%20Cecilia%20M.&rft.date=1998&rft.volume=37&rft.issue=4-5&rft.spage=125&rft.epage=129&rft.pages=125-129&rft.issn=0273-1223&rft.eissn=1996-9732&rft_id=info:doi/10.1016/S0273-1223(98)00094-8&rft_dat=%3Cproquest_cross%3E13669428%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1943311859&rft_id=info:pmid/&rft_els_id=S0273122398000948&rfr_iscdi=true |