Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells
Breast cancer is a heterogeneous disease with distinct subtypes that have made targeted therapy of breast cancer challenging. Previous studies have demonstrated that an altered autophagy capacity can influence the development of breast cancer. However, the molecular differences in starvation-induced...
Gespeichert in:
| Veröffentlicht in: | Animal cells and systems 2017, 21(3), , pp.190-198 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 198 |
|---|---|
| container_issue | 3 |
| container_start_page | 190 |
| container_title | Animal cells and systems |
| container_volume | 21 |
| creator | Zhu, Wanyun Qu, Hao Xu, Kaixiang Jia, Baoyu Li, Haifeng Du, Yimin Liu, Guangming Wei, Hong-Jiang Zhao, Hong-Ye |
| description | Breast cancer is a heterogeneous disease with distinct subtypes that have made targeted therapy of breast cancer challenging. Previous studies have demonstrated that an altered autophagy capacity can influence the development of breast cancer. However, the molecular differences in starvation-induced autophagic responses in MDA-MB-231 and MCF-7 cells have not been fully elucidated. In this study, we found that an increase of LC3B-II protein expression level and a decrease of the p62 protein expression level in both cells treated by Earle's balanced salt solution. Meanwhile, we observed an increase of autophagosome using transmission electron microscopy and an enhancement in the green fluorescence intensity of LC3B protein by confocal microscopy. Furthermore, we detected the expression of 13 autophagy-related (ATG) genes and 11 autophagy signaling pathway-related genes using qPCR. Among 13 ATG genes, we found that 6 genes were up-regulated in treated MDA-MB-231 cells, while 4 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. In addition, among 11 autophagy signaling pathway-related genes, 7 genes were up-regulated in treated MDA-MB-231 cells, while 5 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. These findings suggest that the autophagic response to starvation was different in the two treated cell lines, which will contribute to further study on the molecular mechanism of starvation-induced autophagy and improve the targeted therapy of breast cancer. |
| doi_str_mv | 10.1080/19768354.2017.1330763 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2136551724</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2136551724</sourcerecordid><originalsourceid>FETCH-LOGICAL-c596t-e28d0cdc66fbbd610f81ccf92f0bc44c72cb81c3f05a7192ec7713b79a7f637a3</originalsourceid><addsrcrecordid>eNp9kU9vFCEYh4nR2LX6ETQkXvQwK38GGC7GdWttk25MTD0ThoEu7SxsYaZmv71Md9toD57eAM_7wMsPgLcYzTFq0CcsBW8oq-cEYTHHlCLB6TMwI5jhitQNew5mE1NN0BF4lfM1QpygRr4ERxTVvKzkDHQn3jmbbDA2Qx_gsLYwDzrd6cHHUPnQjcZ2UI9D3K711Q4mm7cxZDvBq5NFtfpaEYqhDh1cLU8rAdtkdR6g0UWZoLF9n1-DF0732b451GPw6_Tb5fKsuvjx_Xy5uKgMk3yoLGk6ZDrDuWvbjmPkGmyMk8Sh1tS1EcS0ZYc6xLTAklgjBKatkFo4ToWmx-Dj3huSUzfGq6j9fb2K6iapxc_Lc4Vpw2shCvt5z27HdmM7Y8OQdK-2yW902t13_nsS_Lp47hQvBsomwYeDIMXb0eZBbXyextXBxjErgilnDAtSF_T9E_Q6jimUr1BYIklYU0tWKLanTIo5J-seH4ORmjJXD5mrKXN1yLz0vft7kseuh5AL8GUP-OBi2ujfMfWdGvSuj8mlkpPPiv7_jj973rpL</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1909258495</pqid></control><display><type>article</type><title>Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells</title><source>DOAJ Directory of Open Access Journals</source><source>Access via Taylor & Francis (Open Access Collection)</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Zhu, Wanyun ; Qu, Hao ; Xu, Kaixiang ; Jia, Baoyu ; Li, Haifeng ; Du, Yimin ; Liu, Guangming ; Wei, Hong-Jiang ; Zhao, Hong-Ye</creator><creatorcontrib>Zhu, Wanyun ; Qu, Hao ; Xu, Kaixiang ; Jia, Baoyu ; Li, Haifeng ; Du, Yimin ; Liu, Guangming ; Wei, Hong-Jiang ; Zhao, Hong-Ye</creatorcontrib><description>Breast cancer is a heterogeneous disease with distinct subtypes that have made targeted therapy of breast cancer challenging. Previous studies have demonstrated that an altered autophagy capacity can influence the development of breast cancer. However, the molecular differences in starvation-induced autophagic responses in MDA-MB-231 and MCF-7 cells have not been fully elucidated. In this study, we found that an increase of LC3B-II protein expression level and a decrease of the p62 protein expression level in both cells treated by Earle's balanced salt solution. Meanwhile, we observed an increase of autophagosome using transmission electron microscopy and an enhancement in the green fluorescence intensity of LC3B protein by confocal microscopy. Furthermore, we detected the expression of 13 autophagy-related (ATG) genes and 11 autophagy signaling pathway-related genes using qPCR. Among 13 ATG genes, we found that 6 genes were up-regulated in treated MDA-MB-231 cells, while 4 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. In addition, among 11 autophagy signaling pathway-related genes, 7 genes were up-regulated in treated MDA-MB-231 cells, while 5 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. These findings suggest that the autophagic response to starvation was different in the two treated cell lines, which will contribute to further study on the molecular mechanism of starvation-induced autophagy and improve the targeted therapy of breast cancer.</description><identifier>ISSN: 1976-8354</identifier><identifier>EISSN: 2151-2485</identifier><identifier>DOI: 10.1080/19768354.2017.1330763</identifier><identifier>PMID: 30460069</identifier><language>eng</language><publisher>Korea (South): Taylor & Francis</publisher><subject>Autophagy ; autophagy signaling pathway-related genes ; autophagy-related genes ; Biotechnology ; Breast cancer ; Cancer ; Confocal microscopy ; Electron microscopy ; Fluorescence ; Gene expression ; Genes ; MCF-7 ; MDA-MB-231 ; p62 Protein ; Phagocytosis ; Protein expression ; Proteins ; Salts ; Signal transduction ; starvation ; Therapy ; Transmission electron microscopy ; 생물학</subject><ispartof>Animal Cells and Systems, 2017, 21(3), , pp.190-198</ispartof><rights>2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2017</rights><rights>2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group</rights><rights>2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2017 The Author(s)</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c596t-e28d0cdc66fbbd610f81ccf92f0bc44c72cb81c3f05a7192ec7713b79a7f637a3</citedby><cites>FETCH-LOGICAL-c596t-e28d0cdc66fbbd610f81ccf92f0bc44c72cb81c3f05a7192ec7713b79a7f637a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138357/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138357/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,729,782,786,866,887,27509,27931,27932,53798,53800,59150,59151</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30460069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiView.kci?sereArticleSearchBean.artiId=ART002231552$$DAccess content in National Research Foundation of Korea (NRF)$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Wanyun</creatorcontrib><creatorcontrib>Qu, Hao</creatorcontrib><creatorcontrib>Xu, Kaixiang</creatorcontrib><creatorcontrib>Jia, Baoyu</creatorcontrib><creatorcontrib>Li, Haifeng</creatorcontrib><creatorcontrib>Du, Yimin</creatorcontrib><creatorcontrib>Liu, Guangming</creatorcontrib><creatorcontrib>Wei, Hong-Jiang</creatorcontrib><creatorcontrib>Zhao, Hong-Ye</creatorcontrib><title>Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells</title><title>Animal cells and systems</title><addtitle>Animal Cells Syst (Seoul)</addtitle><description>Breast cancer is a heterogeneous disease with distinct subtypes that have made targeted therapy of breast cancer challenging. Previous studies have demonstrated that an altered autophagy capacity can influence the development of breast cancer. However, the molecular differences in starvation-induced autophagic responses in MDA-MB-231 and MCF-7 cells have not been fully elucidated. In this study, we found that an increase of LC3B-II protein expression level and a decrease of the p62 protein expression level in both cells treated by Earle's balanced salt solution. Meanwhile, we observed an increase of autophagosome using transmission electron microscopy and an enhancement in the green fluorescence intensity of LC3B protein by confocal microscopy. Furthermore, we detected the expression of 13 autophagy-related (ATG) genes and 11 autophagy signaling pathway-related genes using qPCR. Among 13 ATG genes, we found that 6 genes were up-regulated in treated MDA-MB-231 cells, while 4 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. In addition, among 11 autophagy signaling pathway-related genes, 7 genes were up-regulated in treated MDA-MB-231 cells, while 5 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. These findings suggest that the autophagic response to starvation was different in the two treated cell lines, which will contribute to further study on the molecular mechanism of starvation-induced autophagy and improve the targeted therapy of breast cancer.</description><subject>Autophagy</subject><subject>autophagy signaling pathway-related genes</subject><subject>autophagy-related genes</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Confocal microscopy</subject><subject>Electron microscopy</subject><subject>Fluorescence</subject><subject>Gene expression</subject><subject>Genes</subject><subject>MCF-7</subject><subject>MDA-MB-231</subject><subject>p62 Protein</subject><subject>Phagocytosis</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Salts</subject><subject>Signal transduction</subject><subject>starvation</subject><subject>Therapy</subject><subject>Transmission electron microscopy</subject><subject>생물학</subject><issn>1976-8354</issn><issn>2151-2485</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><recordid>eNp9kU9vFCEYh4nR2LX6ETQkXvQwK38GGC7GdWttk25MTD0ThoEu7SxsYaZmv71Md9toD57eAM_7wMsPgLcYzTFq0CcsBW8oq-cEYTHHlCLB6TMwI5jhitQNew5mE1NN0BF4lfM1QpygRr4ERxTVvKzkDHQn3jmbbDA2Qx_gsLYwDzrd6cHHUPnQjcZ2UI9D3K711Q4mm7cxZDvBq5NFtfpaEYqhDh1cLU8rAdtkdR6g0UWZoLF9n1-DF0732b451GPw6_Tb5fKsuvjx_Xy5uKgMk3yoLGk6ZDrDuWvbjmPkGmyMk8Sh1tS1EcS0ZYc6xLTAklgjBKatkFo4ToWmx-Dj3huSUzfGq6j9fb2K6iapxc_Lc4Vpw2shCvt5z27HdmM7Y8OQdK-2yW902t13_nsS_Lp47hQvBsomwYeDIMXb0eZBbXyextXBxjErgilnDAtSF_T9E_Q6jimUr1BYIklYU0tWKLanTIo5J-seH4ORmjJXD5mrKXN1yLz0vft7kseuh5AL8GUP-OBi2ujfMfWdGvSuj8mlkpPPiv7_jj973rpL</recordid><startdate>20170517</startdate><enddate>20170517</enddate><creator>Zhu, Wanyun</creator><creator>Qu, Hao</creator><creator>Xu, Kaixiang</creator><creator>Jia, Baoyu</creator><creator>Li, Haifeng</creator><creator>Du, Yimin</creator><creator>Liu, Guangming</creator><creator>Wei, Hong-Jiang</creator><creator>Zhao, Hong-Ye</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>한국통합생물학회</general><scope>0YH</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7SN</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>ACYCR</scope></search><sort><creationdate>20170517</creationdate><title>Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells</title><author>Zhu, Wanyun ; Qu, Hao ; Xu, Kaixiang ; Jia, Baoyu ; Li, Haifeng ; Du, Yimin ; Liu, Guangming ; Wei, Hong-Jiang ; Zhao, Hong-Ye</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c596t-e28d0cdc66fbbd610f81ccf92f0bc44c72cb81c3f05a7192ec7713b79a7f637a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Autophagy</topic><topic>autophagy signaling pathway-related genes</topic><topic>autophagy-related genes</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Confocal microscopy</topic><topic>Electron microscopy</topic><topic>Fluorescence</topic><topic>Gene expression</topic><topic>Genes</topic><topic>MCF-7</topic><topic>MDA-MB-231</topic><topic>p62 Protein</topic><topic>Phagocytosis</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Salts</topic><topic>Signal transduction</topic><topic>starvation</topic><topic>Therapy</topic><topic>Transmission electron microscopy</topic><topic>생물학</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Wanyun</creatorcontrib><creatorcontrib>Qu, Hao</creatorcontrib><creatorcontrib>Xu, Kaixiang</creatorcontrib><creatorcontrib>Jia, Baoyu</creatorcontrib><creatorcontrib>Li, Haifeng</creatorcontrib><creatorcontrib>Du, Yimin</creatorcontrib><creatorcontrib>Liu, Guangming</creatorcontrib><creatorcontrib>Wei, Hong-Jiang</creatorcontrib><creatorcontrib>Zhao, Hong-Ye</creatorcontrib><collection>Access via Taylor & Francis (Open Access Collection)</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Korean Citation Index</collection><jtitle>Animal cells and systems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Wanyun</au><au>Qu, Hao</au><au>Xu, Kaixiang</au><au>Jia, Baoyu</au><au>Li, Haifeng</au><au>Du, Yimin</au><au>Liu, Guangming</au><au>Wei, Hong-Jiang</au><au>Zhao, Hong-Ye</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells</atitle><jtitle>Animal cells and systems</jtitle><addtitle>Animal Cells Syst (Seoul)</addtitle><date>2017-05-17</date><risdate>2017</risdate><volume>21</volume><issue>3</issue><spage>190</spage><epage>198</epage><pages>190-198</pages><issn>1976-8354</issn><eissn>2151-2485</eissn><abstract>Breast cancer is a heterogeneous disease with distinct subtypes that have made targeted therapy of breast cancer challenging. Previous studies have demonstrated that an altered autophagy capacity can influence the development of breast cancer. However, the molecular differences in starvation-induced autophagic responses in MDA-MB-231 and MCF-7 cells have not been fully elucidated. In this study, we found that an increase of LC3B-II protein expression level and a decrease of the p62 protein expression level in both cells treated by Earle's balanced salt solution. Meanwhile, we observed an increase of autophagosome using transmission electron microscopy and an enhancement in the green fluorescence intensity of LC3B protein by confocal microscopy. Furthermore, we detected the expression of 13 autophagy-related (ATG) genes and 11 autophagy signaling pathway-related genes using qPCR. Among 13 ATG genes, we found that 6 genes were up-regulated in treated MDA-MB-231 cells, while 4 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. In addition, among 11 autophagy signaling pathway-related genes, 7 genes were up-regulated in treated MDA-MB-231 cells, while 5 genes were up-regulated and 1 gene was down-regulated in treated MCF-7 cells. These findings suggest that the autophagic response to starvation was different in the two treated cell lines, which will contribute to further study on the molecular mechanism of starvation-induced autophagy and improve the targeted therapy of breast cancer.</abstract><cop>Korea (South)</cop><pub>Taylor & Francis</pub><pmid>30460069</pmid><doi>10.1080/19768354.2017.1330763</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1976-8354 |
| ispartof | Animal Cells and Systems, 2017, 21(3), , pp.190-198 |
| issn | 1976-8354 2151-2485 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2136551724 |
| source | DOAJ Directory of Open Access Journals; Access via Taylor & Francis (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | Autophagy autophagy signaling pathway-related genes autophagy-related genes Biotechnology Breast cancer Cancer Confocal microscopy Electron microscopy Fluorescence Gene expression Genes MCF-7 MDA-MB-231 p62 Protein Phagocytosis Protein expression Proteins Salts Signal transduction starvation Therapy Transmission electron microscopy 생물학 |
| title | Differences in the starvation-induced autophagy response in MDA-MB-231 and MCF-7 breast cancer cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T06%3A31%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differences%20in%20the%20starvation-induced%20autophagy%20response%20in%20MDA-MB-231%20and%20MCF-7%20breast%20cancer%20cells&rft.jtitle=Animal%20cells%20and%20systems&rft.au=Zhu,%20Wanyun&rft.date=2017-05-17&rft.volume=21&rft.issue=3&rft.spage=190&rft.epage=198&rft.pages=190-198&rft.issn=1976-8354&rft.eissn=2151-2485&rft_id=info:doi/10.1080/19768354.2017.1330763&rft_dat=%3Cproquest_cross%3E2136551724%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1909258495&rft_id=info:pmid/30460069&rfr_iscdi=true |