Natural Trypanosoma (Trypanozoon) evansi (Steel, 1885) infection among mammals from Brazilian Amazon

First description of Trypanosoma evansi infection in dogs, bats and capybaras from Acre state and the first study that confirms the parasite through molecular sequence analysis in the Brazilian Amazon. [Display omitted] •Anti-T. evansi and anti-T. cruzi antibodies detected in 20.5% and 26% of dogs.•T. evansi infection serologically confirmed in 17.4% of the 46 evaluated capybaras.•T. evansi DNA detected in dog and bat using a nuclear target of T. (Trypanozoon) sp.•DNA sequences from dog and bat showed 89% of similarity with T. brucei satellite DNA. Trypanosoma evansi (Kinetoplastea Trypanosomatidae) is the Trypanosoma species that infects the greatest variety of mammals worldwide. In 2014, a dog from Rio Branco/AC, in the Brazilian Amazon region, presented flagellates without evident kinetoplasts in blood and symptoms of T. evansi infection. Our aim was to investigate the occurrence of T. evansi in dogs, bats and capybaras from Rio Branco. Blood was collected from 78 dogs from residential areas near the Zoobotanical Park (PZ). The serological diagnosis by IFAT detected the presence of anti-T. evansi antibodies in 21.9% of the evaluated dogs. T. evansi DNA was detected in one dog using a higly specific target of a repeated monomer of the satellite DNA of Trypanosoma (Trypanozoon) sp. Molecular diagnosis was also performed on 182 bat spleen samples collected inside PZ, and one Carollia perspicillata was positive. The DNA sequences obtained from these two samples showed similarities with T. brucei satellite DNA. Anti-T. evansi IFAT was carried out in 46 capybaras from rural and urban areas and the infection detected in 17.4% of them. We confirmed for the first time the presence of T. evansi in Acre State and describe three putative host species involved in the parasite transmission in that Amazon region. Moreover, this is the first study that confirms the infection by T. evansi through DNA sequence analysis in the Brazilian Amazon Region.