Transcriptome analysis of immune response in fat greenling (Hexagrammos otakii) against Vibrio harveyi infection
Fat greenling (Hexagrammos otakii) is an important aquaculture fish species in northern China. Unfortunately, Vibrio infections have caused considerable losses to the fat greenling aquaculture industry. However, the study on immune response of fat greenling against Vibrio species has not been report...
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| Veröffentlicht in: | Fish & shellfish immunology 2019-01, Vol.84, p.937-947 |
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| creator | Diao, Jing Liu, Hongjun Hu, Fawen Li, Le Wang, Xiaolu Gai, Chunlei Yu, Xiaoqing Fan, Ying Xu, La Ye, Haibin |
| description | Fat greenling (Hexagrammos otakii) is an important aquaculture fish species in northern China. Unfortunately, Vibrio infections have caused considerable losses to the fat greenling aquaculture industry. However, the study on immune response of fat greenling against Vibrio species has not been reported yet. In this paper, the immune response of fat greenling against V. harveyi at gene expression level was studied by transcriptome analysis. A total of 189753 high-quality unigenes with a N50 length of 672bp were obtained by transcriptome profiling, which provided abundant data for the future study of fat greenling. Comparative analysis showed that 5425 differentially expressed genes (DEGs) were identified on day 3 post-infection (3dpi), containing 1837 up-regulated and 3588 down-regulated genes. Further annotation and analysis revealed that the DEGs were enriched in complement and coagulation cascades, ribosome, oxidative phosphorylation, glycine, serine and threonine metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways were mainly associated with phagocytosis and pathogen clearance, rarely involved in bacteria adhesion and pathogen identification, which suggested that the host might begin to clear and kill the invading bacteria on 3dpi. The research might provide a valuable resource to further study immune response and suggest strategies against V. harveyi infection in fat greenling.
•The immune response of fat greenling against Vibrio harveyi infection was determined by RNA-seq.•A total of 189753 high-quality unigenes were obtained by transcriptome profiling.•5425 DEGs were identified on day 3 post-infection containing 1837 up-regulated and 3588 down-regulated genes.•DEGs were enriched in complement and coagulation cascades signaling pathway. |
| doi_str_mv | 10.1016/j.fsi.2018.10.067 |
| format | Article |
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•The immune response of fat greenling against Vibrio harveyi infection was determined by RNA-seq.•A total of 189753 high-quality unigenes were obtained by transcriptome profiling.•5425 DEGs were identified on day 3 post-infection containing 1837 up-regulated and 3588 down-regulated genes.•DEGs were enriched in complement and coagulation cascades signaling pathway.</description><identifier>ISSN: 1050-4648</identifier><identifier>EISSN: 1095-9947</identifier><identifier>DOI: 10.1016/j.fsi.2018.10.067</identifier><identifier>PMID: 30445666</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; aquaculture ; aquaculture industry ; bacteria ; bacterial adhesion ; China ; coagulation ; complement ; Fat greenling (Hexagrammos otakii) ; fish ; Fish Diseases - genetics ; Fish Diseases - immunology ; Fish Diseases - microbiology ; gene expression ; Gene Expression Profiling - veterinary ; gene expression regulation ; Hexagrammos otakii ; Immune response ; Immunity, Innate ; oxidative phosphorylation ; pathogen identification ; pathogens ; Perciformes ; peroxisome proliferator-activated receptors ; phagocytosis ; ribosomes ; serine ; shellfish ; signal transduction ; threonine ; Transcriptome ; transcriptomics ; unigenes ; Vibrio - physiology ; Vibrio harveyi ; Vibrio Infections - genetics ; Vibrio Infections - immunology ; Vibrio Infections - microbiology ; Vibrio Infections - veterinary</subject><ispartof>Fish & shellfish immunology, 2019-01, Vol.84, p.937-947</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-bdf324441a6addc3b97265ffc1b6ddb92ec14ddbe2d09ba96e73f34b641f948b3</citedby><cites>FETCH-LOGICAL-c386t-bdf324441a6addc3b97265ffc1b6ddb92ec14ddbe2d09ba96e73f34b641f948b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1050464818307009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30445666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Diao, Jing</creatorcontrib><creatorcontrib>Liu, Hongjun</creatorcontrib><creatorcontrib>Hu, Fawen</creatorcontrib><creatorcontrib>Li, Le</creatorcontrib><creatorcontrib>Wang, Xiaolu</creatorcontrib><creatorcontrib>Gai, Chunlei</creatorcontrib><creatorcontrib>Yu, Xiaoqing</creatorcontrib><creatorcontrib>Fan, Ying</creatorcontrib><creatorcontrib>Xu, La</creatorcontrib><creatorcontrib>Ye, Haibin</creatorcontrib><title>Transcriptome analysis of immune response in fat greenling (Hexagrammos otakii) against Vibrio harveyi infection</title><title>Fish & shellfish immunology</title><addtitle>Fish Shellfish Immunol</addtitle><description>Fat greenling (Hexagrammos otakii) is an important aquaculture fish species in northern China. Unfortunately, Vibrio infections have caused considerable losses to the fat greenling aquaculture industry. However, the study on immune response of fat greenling against Vibrio species has not been reported yet. In this paper, the immune response of fat greenling against V. harveyi at gene expression level was studied by transcriptome analysis. A total of 189753 high-quality unigenes with a N50 length of 672bp were obtained by transcriptome profiling, which provided abundant data for the future study of fat greenling. Comparative analysis showed that 5425 differentially expressed genes (DEGs) were identified on day 3 post-infection (3dpi), containing 1837 up-regulated and 3588 down-regulated genes. Further annotation and analysis revealed that the DEGs were enriched in complement and coagulation cascades, ribosome, oxidative phosphorylation, glycine, serine and threonine metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways were mainly associated with phagocytosis and pathogen clearance, rarely involved in bacteria adhesion and pathogen identification, which suggested that the host might begin to clear and kill the invading bacteria on 3dpi. The research might provide a valuable resource to further study immune response and suggest strategies against V. harveyi infection in fat greenling.
•The immune response of fat greenling against Vibrio harveyi infection was determined by RNA-seq.•A total of 189753 high-quality unigenes were obtained by transcriptome profiling.•5425 DEGs were identified on day 3 post-infection containing 1837 up-regulated and 3588 down-regulated genes.•DEGs were enriched in complement and coagulation cascades signaling pathway.</description><subject>Animals</subject><subject>aquaculture</subject><subject>aquaculture industry</subject><subject>bacteria</subject><subject>bacterial adhesion</subject><subject>China</subject><subject>coagulation</subject><subject>complement</subject><subject>Fat greenling (Hexagrammos otakii)</subject><subject>fish</subject><subject>Fish Diseases - genetics</subject><subject>Fish Diseases - immunology</subject><subject>Fish Diseases - microbiology</subject><subject>gene expression</subject><subject>Gene Expression Profiling - veterinary</subject><subject>gene expression regulation</subject><subject>Hexagrammos otakii</subject><subject>Immune response</subject><subject>Immunity, Innate</subject><subject>oxidative phosphorylation</subject><subject>pathogen identification</subject><subject>pathogens</subject><subject>Perciformes</subject><subject>peroxisome proliferator-activated receptors</subject><subject>phagocytosis</subject><subject>ribosomes</subject><subject>serine</subject><subject>shellfish</subject><subject>signal transduction</subject><subject>threonine</subject><subject>Transcriptome</subject><subject>transcriptomics</subject><subject>unigenes</subject><subject>Vibrio - physiology</subject><subject>Vibrio harveyi</subject><subject>Vibrio Infections - genetics</subject><subject>Vibrio Infections - immunology</subject><subject>Vibrio Infections - microbiology</subject><subject>Vibrio Infections - veterinary</subject><issn>1050-4648</issn><issn>1095-9947</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEoqXwA7ggH8shix07TixOqCoUqRKXwtXyx3iZJbGDna3Yf49X23IsJ49Hz_se5mmat4xuGGXyw24TCm46ysb631A5PGvOGVV9q5QYnh_nnrZCivGseVXKjlIquaQvmzNOheillOfNcpdNLC7jsqYZiIlmOhQsJAWC87yPQDKUJcUCBCMJZiXbDBAnjFtyeQN_zDabeU41sJpfiO-J2RqMZSU_0GZM5KfJ93DAGg7gVkzxdfMimKnAm4f3ovn--fru6qa9_fbl69Wn29bxUa6t9YF3QghmpPHecauGTvYhOGal91Z14JioA3SeKmuUhIEHLqwULCgxWn7RXJ56l5x-76GsesbiYJpMhLQvuusGNo6dUOz_KOM942wYVUXZCXU5lZIh6CXjbPJBM6qPTvROVyf66OS4qk5q5t1D_d7O4P8lHiVU4OMJgHqPe4Ssi0OIDjzmejTtEz5R_xeA-p7v</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Diao, Jing</creator><creator>Liu, Hongjun</creator><creator>Hu, Fawen</creator><creator>Li, Le</creator><creator>Wang, Xiaolu</creator><creator>Gai, Chunlei</creator><creator>Yu, Xiaoqing</creator><creator>Fan, Ying</creator><creator>Xu, La</creator><creator>Ye, Haibin</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>201901</creationdate><title>Transcriptome analysis of immune response in fat greenling (Hexagrammos otakii) against Vibrio harveyi infection</title><author>Diao, Jing ; Liu, Hongjun ; Hu, Fawen ; Li, Le ; Wang, Xiaolu ; Gai, Chunlei ; Yu, Xiaoqing ; Fan, Ying ; Xu, La ; Ye, Haibin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-bdf324441a6addc3b97265ffc1b6ddb92ec14ddbe2d09ba96e73f34b641f948b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>aquaculture</topic><topic>aquaculture industry</topic><topic>bacteria</topic><topic>bacterial adhesion</topic><topic>China</topic><topic>coagulation</topic><topic>complement</topic><topic>Fat greenling (Hexagrammos otakii)</topic><topic>fish</topic><topic>Fish Diseases - genetics</topic><topic>Fish Diseases - immunology</topic><topic>Fish Diseases - microbiology</topic><topic>gene expression</topic><topic>Gene Expression Profiling - veterinary</topic><topic>gene expression regulation</topic><topic>Hexagrammos otakii</topic><topic>Immune response</topic><topic>Immunity, Innate</topic><topic>oxidative phosphorylation</topic><topic>pathogen identification</topic><topic>pathogens</topic><topic>Perciformes</topic><topic>peroxisome proliferator-activated receptors</topic><topic>phagocytosis</topic><topic>ribosomes</topic><topic>serine</topic><topic>shellfish</topic><topic>signal transduction</topic><topic>threonine</topic><topic>Transcriptome</topic><topic>transcriptomics</topic><topic>unigenes</topic><topic>Vibrio - physiology</topic><topic>Vibrio harveyi</topic><topic>Vibrio Infections - genetics</topic><topic>Vibrio Infections - immunology</topic><topic>Vibrio Infections - microbiology</topic><topic>Vibrio Infections - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Diao, Jing</creatorcontrib><creatorcontrib>Liu, Hongjun</creatorcontrib><creatorcontrib>Hu, Fawen</creatorcontrib><creatorcontrib>Li, Le</creatorcontrib><creatorcontrib>Wang, Xiaolu</creatorcontrib><creatorcontrib>Gai, Chunlei</creatorcontrib><creatorcontrib>Yu, Xiaoqing</creatorcontrib><creatorcontrib>Fan, Ying</creatorcontrib><creatorcontrib>Xu, La</creatorcontrib><creatorcontrib>Ye, Haibin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Fish & shellfish immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Diao, Jing</au><au>Liu, Hongjun</au><au>Hu, Fawen</au><au>Li, Le</au><au>Wang, Xiaolu</au><au>Gai, Chunlei</au><au>Yu, Xiaoqing</au><au>Fan, Ying</au><au>Xu, La</au><au>Ye, Haibin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptome analysis of immune response in fat greenling (Hexagrammos otakii) against Vibrio harveyi infection</atitle><jtitle>Fish & shellfish immunology</jtitle><addtitle>Fish Shellfish Immunol</addtitle><date>2019-01</date><risdate>2019</risdate><volume>84</volume><spage>937</spage><epage>947</epage><pages>937-947</pages><issn>1050-4648</issn><eissn>1095-9947</eissn><abstract>Fat greenling (Hexagrammos otakii) is an important aquaculture fish species in northern China. Unfortunately, Vibrio infections have caused considerable losses to the fat greenling aquaculture industry. However, the study on immune response of fat greenling against Vibrio species has not been reported yet. In this paper, the immune response of fat greenling against V. harveyi at gene expression level was studied by transcriptome analysis. A total of 189753 high-quality unigenes with a N50 length of 672bp were obtained by transcriptome profiling, which provided abundant data for the future study of fat greenling. Comparative analysis showed that 5425 differentially expressed genes (DEGs) were identified on day 3 post-infection (3dpi), containing 1837 up-regulated and 3588 down-regulated genes. Further annotation and analysis revealed that the DEGs were enriched in complement and coagulation cascades, ribosome, oxidative phosphorylation, glycine, serine and threonine metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways were mainly associated with phagocytosis and pathogen clearance, rarely involved in bacteria adhesion and pathogen identification, which suggested that the host might begin to clear and kill the invading bacteria on 3dpi. The research might provide a valuable resource to further study immune response and suggest strategies against V. harveyi infection in fat greenling.
•The immune response of fat greenling against Vibrio harveyi infection was determined by RNA-seq.•A total of 189753 high-quality unigenes were obtained by transcriptome profiling.•5425 DEGs were identified on day 3 post-infection containing 1837 up-regulated and 3588 down-regulated genes.•DEGs were enriched in complement and coagulation cascades signaling pathway.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30445666</pmid><doi>10.1016/j.fsi.2018.10.067</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals aquaculture aquaculture industry bacteria bacterial adhesion China coagulation complement Fat greenling (Hexagrammos otakii) fish Fish Diseases - genetics Fish Diseases - immunology Fish Diseases - microbiology gene expression Gene Expression Profiling - veterinary gene expression regulation Hexagrammos otakii Immune response Immunity, Innate oxidative phosphorylation pathogen identification pathogens Perciformes peroxisome proliferator-activated receptors phagocytosis ribosomes serine shellfish signal transduction threonine Transcriptome transcriptomics unigenes Vibrio - physiology Vibrio harveyi Vibrio Infections - genetics Vibrio Infections - immunology Vibrio Infections - microbiology Vibrio Infections - veterinary |
| title | Transcriptome analysis of immune response in fat greenling (Hexagrammos otakii) against Vibrio harveyi infection |
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