Evaluating the association of TNF α promoter haplotype with its serum levels and the risk of PCOS: A case control study

•TNF α levels were measured in different PCOS phenotypes and control.•Association of TNF α levels with different promoter polymorphisms is checked.•Haplotype analysis predicted most susceptible genotype having increased TNF α levels.•Most susceptible genotype for PCOS risk was also predicted by Hapl...

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Veröffentlicht in:Cytokine (Philadelphia, Pa.) Pa.), 2019-02, Vol.114, p.86-91
Hauptverfasser: Bhatnager, Richa, Jalthuria, Jitesh, Sehrawat, Ravinder, Nanda, Smiti, Dang, Amita S.
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container_title Cytokine (Philadelphia, Pa.)
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creator Bhatnager, Richa
Jalthuria, Jitesh
Sehrawat, Ravinder
Nanda, Smiti
Dang, Amita S.
description •TNF α levels were measured in different PCOS phenotypes and control.•Association of TNF α levels with different promoter polymorphisms is checked.•Haplotype analysis predicted most susceptible genotype having increased TNF α levels.•Most susceptible genotype for PCOS risk was also predicted by Haplotype analysis. Poly cystic ovary syndrome is the major cause of anovulatory infertility. TNF α, pro-inflammatory cytokine is associated with obesity, insulin resistance and hyperandrogenism, therefore in present study we tried to find the association between TNF α promoter polymorphisms, TNF α levels and the risk of PCOS. Present case control study was carried on 400 women of age 16–40 years. TNF α levels were measured by ELISA whereas promoter polymorphisms were evaluated by PCR-RFLP. Haplotype and Linkage disequilibrium analysis was also done. TNF α level was significantly higher in PCOS group (13.24 ± 9.78) than control (5.5 ± 3.8). Haplotype analysis revealed that GGTCT, AGTCT, AGCCT and AACCT are the susceptible haplotypes associated with TNF α level. rs361525 and rs1799964 were found to be associated with the risk of PCOS (p = 0.0006, 0.015). GGCCT, AATAT, GATAT (most susceptible), AGCCT, GGTCT and GATCT are the susceptible haplotypes for PCOS. Significant difference between TNF α levels in PCOS and Control group suggest it's one of the promising candidates for the marker of inflammation (sensitivity and specificity 91.23 and 94.56% respectively, with area under the curve 0.907 at 95% CI 0.8723–0.9512). Presence of GGCCT haplotype suggests the susceptibility towards PCOS which needs to be further verified. In addition to this, present study not only provides a pavement for the diagnosis, but also monitoring and management of PCOS too.
doi_str_mv 10.1016/j.cyto.2018.11.004
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Poly cystic ovary syndrome is the major cause of anovulatory infertility. TNF α, pro-inflammatory cytokine is associated with obesity, insulin resistance and hyperandrogenism, therefore in present study we tried to find the association between TNF α promoter polymorphisms, TNF α levels and the risk of PCOS. Present case control study was carried on 400 women of age 16–40 years. TNF α levels were measured by ELISA whereas promoter polymorphisms were evaluated by PCR-RFLP. Haplotype and Linkage disequilibrium analysis was also done. TNF α level was significantly higher in PCOS group (13.24 ± 9.78) than control (5.5 ± 3.8). Haplotype analysis revealed that GGTCT, AGTCT, AGCCT and AACCT are the susceptible haplotypes associated with TNF α level. rs361525 and rs1799964 were found to be associated with the risk of PCOS (p = 0.0006, 0.015). GGCCT, AATAT, GATAT (most susceptible), AGCCT, GGTCT and GATCT are the susceptible haplotypes for PCOS. Significant difference between TNF α levels in PCOS and Control group suggest it's one of the promising candidates for the marker of inflammation (sensitivity and specificity 91.23 and 94.56% respectively, with area under the curve 0.907 at 95% CI 0.8723–0.9512). Presence of GGCCT haplotype suggests the susceptibility towards PCOS which needs to be further verified. 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Poly cystic ovary syndrome is the major cause of anovulatory infertility. TNF α, pro-inflammatory cytokine is associated with obesity, insulin resistance and hyperandrogenism, therefore in present study we tried to find the association between TNF α promoter polymorphisms, TNF α levels and the risk of PCOS. Present case control study was carried on 400 women of age 16–40 years. TNF α levels were measured by ELISA whereas promoter polymorphisms were evaluated by PCR-RFLP. Haplotype and Linkage disequilibrium analysis was also done. TNF α level was significantly higher in PCOS group (13.24 ± 9.78) than control (5.5 ± 3.8). Haplotype analysis revealed that GGTCT, AGTCT, AGCCT and AACCT are the susceptible haplotypes associated with TNF α level. rs361525 and rs1799964 were found to be associated with the risk of PCOS (p = 0.0006, 0.015). GGCCT, AATAT, GATAT (most susceptible), AGCCT, GGTCT and GATCT are the susceptible haplotypes for PCOS. Significant difference between TNF α levels in PCOS and Control group suggest it's one of the promising candidates for the marker of inflammation (sensitivity and specificity 91.23 and 94.56% respectively, with area under the curve 0.907 at 95% CI 0.8723–0.9512). Presence of GGCCT haplotype suggests the susceptibility towards PCOS which needs to be further verified. 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Poly cystic ovary syndrome is the major cause of anovulatory infertility. TNF α, pro-inflammatory cytokine is associated with obesity, insulin resistance and hyperandrogenism, therefore in present study we tried to find the association between TNF α promoter polymorphisms, TNF α levels and the risk of PCOS. Present case control study was carried on 400 women of age 16–40 years. TNF α levels were measured by ELISA whereas promoter polymorphisms were evaluated by PCR-RFLP. Haplotype and Linkage disequilibrium analysis was also done. TNF α level was significantly higher in PCOS group (13.24 ± 9.78) than control (5.5 ± 3.8). Haplotype analysis revealed that GGTCT, AGTCT, AGCCT and AACCT are the susceptible haplotypes associated with TNF α level. rs361525 and rs1799964 were found to be associated with the risk of PCOS (p = 0.0006, 0.015). GGCCT, AATAT, GATAT (most susceptible), AGCCT, GGTCT and GATCT are the susceptible haplotypes for PCOS. 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subjects Adult
Case-Control Studies
Female
Gene Frequency - genetics
Genetic Predisposition to Disease
Haplotype
Haplotypes - genetics
Humans
Inflammation
Linkage disequilibrium
Linkage Disequilibrium - genetics
PCOS
Polycystic Ovary Syndrome - blood
Polycystic Ovary Syndrome - genetics
Polymorphism
Promoter Regions, Genetic
Risk Factors
TNF α
Tumor Necrosis Factor-alpha - blood
Tumor Necrosis Factor-alpha - genetics
title Evaluating the association of TNF α promoter haplotype with its serum levels and the risk of PCOS: A case control study
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