Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica
Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the os...
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| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2019-02, Vol.21 (1), p.65-75 |
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| creator | Gabbai-Armelin, P. R. Kido, H. W. Cruz, M. A. Prado, J. P. S. Avanzi, I. R. Custódio, M. R. Renno, A. C. M. Granito, R. N. |
| description | Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications. |
| doi_str_mv | 10.1007/s10126-018-9858-9 |
| format | Article |
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R. ; Kido, H. W. ; Cruz, M. A. ; Prado, J. P. S. ; Avanzi, I. R. ; Custódio, M. R. ; Renno, A. C. M. ; Granito, R. N.</creator><creatorcontrib>Gabbai-Armelin, P. R. ; Kido, H. W. ; Cruz, M. A. ; Prado, J. P. S. ; Avanzi, I. R. ; Custódio, M. R. ; Renno, A. C. M. ; Granito, R. N.</creatorcontrib><description>Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-018-9858-9</identifier><identifier>PMID: 30443837</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Ageing ; Aging ; Aging (natural) ; Animals ; Biocompatibility ; Biocompatible Materials - isolation & purification ; Biocompatible Materials - pharmacology ; Bioglass ; Biomarkers - metabolism ; Biomaterials ; Biomedical and Life Sciences ; Biomedical materials ; Bone and Bones - cytology ; Bone and Bones - drug effects ; Bone and Bones - metabolism ; Bone biomaterials ; Bone grafts ; Bone loss ; Bone morphogenetic protein 4 ; Bone Morphogenetic Protein 4 - genetics ; Bone Morphogenetic Protein 4 - metabolism ; Bone Substitutes - isolation & purification ; Bone Substitutes - pharmacology ; Bones ; Calcium ; Cbfa-1 protein ; Cell Line ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Core Binding Factor Alpha 1 Subunit - genetics ; Core Binding Factor Alpha 1 Subunit - metabolism ; Cytotoxicity ; DNA ; Electron microscopy ; Engineering ; Evaluation ; Fourier transforms ; Fractures ; Fractures, Bone - therapy ; Freshwater & Marine Ecology ; Gene Expression ; Grafts ; Humans ; Infrared spectroscopy ; Life Sciences ; Marine invertebrates ; Mice ; Microbiology ; Mineralization ; Nonunion ; Nucleotide sequence ; Original Article ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Osteoporosis ; PCR ; pH effects ; Porifera - chemistry ; Proliferation ; Properties ; Restoration ; Scanning electron microscopy ; Silicon Dioxide - isolation & purification ; Silicon Dioxide - pharmacology ; Sponges ; Substitute bone ; Tissue ; Tissue engineering ; Tissue Engineering - methods ; Toxicity ; Viability ; X-ray diffraction ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2019-02, Vol.21 (1), p.65-75</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Marine Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-c4a382b8105ac9be18e0a11e30ac394c87d3966e36d0c9f0c556cc3cc5743abc3</citedby><cites>FETCH-LOGICAL-c372t-c4a382b8105ac9be18e0a11e30ac394c87d3966e36d0c9f0c556cc3cc5743abc3</cites><orcidid>0000-0003-4802-0062</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-018-9858-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-018-9858-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,782,786,27931,27932,41495,42564,51326</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30443837$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gabbai-Armelin, P. R.</creatorcontrib><creatorcontrib>Kido, H. W.</creatorcontrib><creatorcontrib>Cruz, M. A.</creatorcontrib><creatorcontrib>Prado, J. P. S.</creatorcontrib><creatorcontrib>Avanzi, I. R.</creatorcontrib><creatorcontrib>Custódio, M. R.</creatorcontrib><creatorcontrib>Renno, A. C. M.</creatorcontrib><creatorcontrib>Granito, R. N.</creatorcontrib><title>Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications.</description><subject>Ageing</subject><subject>Aging</subject><subject>Aging (natural)</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - isolation & purification</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Bioglass</subject><subject>Biomarkers - metabolism</subject><subject>Biomaterials</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical materials</subject><subject>Bone and Bones - cytology</subject><subject>Bone and Bones - drug effects</subject><subject>Bone and Bones - metabolism</subject><subject>Bone biomaterials</subject><subject>Bone grafts</subject><subject>Bone loss</subject><subject>Bone morphogenetic protein 4</subject><subject>Bone Morphogenetic Protein 4 - genetics</subject><subject>Bone Morphogenetic Protein 4 - metabolism</subject><subject>Bone Substitutes - isolation & purification</subject><subject>Bone Substitutes - pharmacology</subject><subject>Bones</subject><subject>Calcium</subject><subject>Cbfa-1 protein</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Core Binding Factor Alpha 1 Subunit - genetics</subject><subject>Core Binding Factor Alpha 1 Subunit - metabolism</subject><subject>Cytotoxicity</subject><subject>DNA</subject><subject>Electron microscopy</subject><subject>Engineering</subject><subject>Evaluation</subject><subject>Fourier transforms</subject><subject>Fractures</subject><subject>Fractures, Bone - therapy</subject><subject>Freshwater & Marine Ecology</subject><subject>Gene Expression</subject><subject>Grafts</subject><subject>Humans</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Marine invertebrates</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Mineralization</subject><subject>Nonunion</subject><subject>Nucleotide sequence</subject><subject>Original Article</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Osteoporosis</subject><subject>PCR</subject><subject>pH effects</subject><subject>Porifera - chemistry</subject><subject>Proliferation</subject><subject>Properties</subject><subject>Restoration</subject><subject>Scanning electron microscopy</subject><subject>Silicon Dioxide - isolation & purification</subject><subject>Silicon Dioxide - pharmacology</subject><subject>Sponges</subject><subject>Substitute bone</subject><subject>Tissue</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Toxicity</subject><subject>Viability</subject><subject>X-ray diffraction</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kEFLwzAYhoMobk5_gBcpePFSTfK1TXvUMXUw8aCeQ_Y11YyumUkrzl9vRucEwUsS-J73_cJDyCmjl4xSceUZZTyLKcvjIk_DsUeGLIEs5hyy_d2b5wNy5P2ChowAekgGQJMEchBDMh2_Kaew1c58qdbYJlJNGY3XrW3tp0HTrqPJh6q7fmarSEUPyplGR08r27zq6MZYb2qD6pgcVKr2-mR7j8jL7eR5fB_PHu-m4-tZjCB4G2OiIOfznNFUYTHXLNdUMaaBKoQiwVyUUGSZhqykWFQU0zRDBMRUJKDmCCNy0feunH3vtG_l0njUda0abTsvOYOU8YSDCOj5H3RhO9eE320oyETKBQ8U6yl01nunK7lyZqncWjIqN55l71kGz3LjWRYhc7Zt7uZLXe4SP2IDwHvAh1Hw5H5X_9_6DZe-h2s</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Gabbai-Armelin, P. 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R.</au><au>Kido, H. W.</au><au>Cruz, M. A.</au><au>Prado, J. P. S.</au><au>Avanzi, I. R.</au><au>Custódio, M. R.</au><au>Renno, A. C. M.</au><au>Granito, R. N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>21</volume><issue>1</issue><spage>65</spage><epage>75</epage><pages>65-75</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30443837</pmid><doi>10.1007/s10126-018-9858-9</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4802-0062</orcidid></addata></record> |
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| ispartof | Marine biotechnology (New York, N.Y.), 2019-02, Vol.21 (1), p.65-75 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_2135124237 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Ageing Aging Aging (natural) Animals Biocompatibility Biocompatible Materials - isolation & purification Biocompatible Materials - pharmacology Bioglass Biomarkers - metabolism Biomaterials Biomedical and Life Sciences Biomedical materials Bone and Bones - cytology Bone and Bones - drug effects Bone and Bones - metabolism Bone biomaterials Bone grafts Bone loss Bone morphogenetic protein 4 Bone Morphogenetic Protein 4 - genetics Bone Morphogenetic Protein 4 - metabolism Bone Substitutes - isolation & purification Bone Substitutes - pharmacology Bones Calcium Cbfa-1 protein Cell Line Cell Proliferation - drug effects Cell Survival - drug effects Core Binding Factor Alpha 1 Subunit - genetics Core Binding Factor Alpha 1 Subunit - metabolism Cytotoxicity DNA Electron microscopy Engineering Evaluation Fourier transforms Fractures Fractures, Bone - therapy Freshwater & Marine Ecology Gene Expression Grafts Humans Infrared spectroscopy Life Sciences Marine invertebrates Mice Microbiology Mineralization Nonunion Nucleotide sequence Original Article Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - metabolism Osteoporosis PCR pH effects Porifera - chemistry Proliferation Properties Restoration Scanning electron microscopy Silicon Dioxide - isolation & purification Silicon Dioxide - pharmacology Sponges Substitute bone Tissue Tissue engineering Tissue Engineering - methods Toxicity Viability X-ray diffraction Zoology |
| title | Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T06%3A12%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20and%20Cytotoxicity%20Evaluation%20of%20a%20Marine%20Sponge%20Biosilica&rft.jtitle=Marine%20biotechnology%20(New%20York,%20N.Y.)&rft.au=Gabbai-Armelin,%20P.%20R.&rft.date=2019-02-01&rft.volume=21&rft.issue=1&rft.spage=65&rft.epage=75&rft.pages=65-75&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s10126-018-9858-9&rft_dat=%3Cproquest_cross%3E2133675272%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2133675272&rft_id=info:pmid/30443837&rfr_iscdi=true |