Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica

Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the os...

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Veröffentlicht in:Marine biotechnology (New York, N.Y.) N.Y.), 2019-02, Vol.21 (1), p.65-75
Hauptverfasser: Gabbai-Armelin, P. R., Kido, H. W., Cruz, M. A., Prado, J. P. S., Avanzi, I. R., Custódio, M. R., Renno, A. C. M., Granito, R. N.
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container_end_page 75
container_issue 1
container_start_page 65
container_title Marine biotechnology (New York, N.Y.)
container_volume 21
creator Gabbai-Armelin, P. R.
Kido, H. W.
Cruz, M. A.
Prado, J. P. S.
Avanzi, I. R.
Custódio, M. R.
Renno, A. C. M.
Granito, R. N.
description Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications.
doi_str_mv 10.1007/s10126-018-9858-9
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R. ; Kido, H. W. ; Cruz, M. A. ; Prado, J. P. S. ; Avanzi, I. R. ; Custódio, M. R. ; Renno, A. C. M. ; Granito, R. N.</creator><creatorcontrib>Gabbai-Armelin, P. R. ; Kido, H. W. ; Cruz, M. A. ; Prado, J. P. S. ; Avanzi, I. R. ; Custódio, M. R. ; Renno, A. C. M. ; Granito, R. N.</creatorcontrib><description>Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. 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R.</creatorcontrib><creatorcontrib>Kido, H. W.</creatorcontrib><creatorcontrib>Cruz, M. A.</creatorcontrib><creatorcontrib>Prado, J. P. S.</creatorcontrib><creatorcontrib>Avanzi, I. R.</creatorcontrib><creatorcontrib>Custódio, M. R.</creatorcontrib><creatorcontrib>Renno, A. C. M.</creatorcontrib><creatorcontrib>Granito, R. N.</creatorcontrib><title>Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. 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R.</au><au>Kido, H. W.</au><au>Cruz, M. A.</au><au>Prado, J. P. S.</au><au>Avanzi, I. R.</au><au>Custódio, M. R.</au><au>Renno, A. C. M.</au><au>Granito, R. N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>21</volume><issue>1</issue><spage>65</spage><epage>75</epage><pages>65-75</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging, and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Thus, the objectives of this study were to study the physicochemical and morphological properties of biosilica (BS) from sponges by using scanning electron microscopy, Fourier-transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mineralization, and pH. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) were performed to investigate its cytotoxicity and proliferation in presence of BS. Bioglass (BG) was used as gold standard material for comparison purposes. Sponge BS was obtained, and this fact was proven by SEM, FTIR, and XRD analysis. Calcium assay showed a progressive release of this ion from day 7 and a more balanced pH for BS was maintained compared to BG. Cytotoxicity assay indicated that BS had a positive influence on MC3T3-E1 cells viability and qRT-PCR showed that this material stimulated Runx2 and BMP4 gene expressions. Taken together, the results indicate a potential use of sponge biosilica for tissue engineering applications.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30443837</pmid><doi>10.1007/s10126-018-9858-9</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-4802-0062</orcidid></addata></record>
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source MEDLINE; SpringerNature Journals
subjects Ageing
Aging
Aging (natural)
Animals
Biocompatibility
Biocompatible Materials - isolation & purification
Biocompatible Materials - pharmacology
Bioglass
Biomarkers - metabolism
Biomaterials
Biomedical and Life Sciences
Biomedical materials
Bone and Bones - cytology
Bone and Bones - drug effects
Bone and Bones - metabolism
Bone biomaterials
Bone grafts
Bone loss
Bone morphogenetic protein 4
Bone Morphogenetic Protein 4 - genetics
Bone Morphogenetic Protein 4 - metabolism
Bone Substitutes - isolation & purification
Bone Substitutes - pharmacology
Bones
Calcium
Cbfa-1 protein
Cell Line
Cell Proliferation - drug effects
Cell Survival - drug effects
Core Binding Factor Alpha 1 Subunit - genetics
Core Binding Factor Alpha 1 Subunit - metabolism
Cytotoxicity
DNA
Electron microscopy
Engineering
Evaluation
Fourier transforms
Fractures
Fractures, Bone - therapy
Freshwater & Marine Ecology
Gene Expression
Grafts
Humans
Infrared spectroscopy
Life Sciences
Marine invertebrates
Mice
Microbiology
Mineralization
Nonunion
Nucleotide sequence
Original Article
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteoporosis
PCR
pH effects
Porifera - chemistry
Proliferation
Properties
Restoration
Scanning electron microscopy
Silicon Dioxide - isolation & purification
Silicon Dioxide - pharmacology
Sponges
Substitute bone
Tissue
Tissue engineering
Tissue Engineering - methods
Toxicity
Viability
X-ray diffraction
Zoology
title Characterization and Cytotoxicity Evaluation of a Marine Sponge Biosilica
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