Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching
The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzym...
Gespeichert in:
Veröffentlicht in: | Journal of the American Chemical Society 2018-12, Vol.140 (49), p.16868-16872 |
---|---|
Hauptverfasser: | , , , , |
Format: | Artikel |
Sprache: | eng |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 16872 |
---|---|
container_issue | 49 |
container_start_page | 16868 |
container_title | Journal of the American Chemical Society |
container_volume | 140 |
creator | Haydell, Michael W Centola, Mathias Adam, Volker Valero, Julián Famulok, Michael |
description | The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices. |
doi_str_mv | 10.1021/jacs.8b08738 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2135119895</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2135119895</sourcerecordid><originalsourceid>FETCH-LOGICAL-a390t-a73ad2371bd4f34528ed431889f2c76c4072fc7bbda64f80e27242e020433a4d3</originalsourceid><addsrcrecordid>eNptkDtPwzAUhS0EoqWwMSOPDKRcPxI7Y1VelSqKUJkjx3HaVElc7AQUfj2pWmBhujrSd86VPoQuCYwJUHK7UdqPZQpSMHmEhiSkEISERsdoCAA0EDJiA3Tm_aaPnEpyigYMOOcRiCGaLU21tU6VWNUZfjUfxvkiLQ2e2rpxtsQ2xwrfPU--usrgtMML16ztytZ942VtG-s_i0avi3p1jk5yVXpzcbgj9PZwv5w-BfPF42w6mQeKxdAESjCVUSZImvGc8ZBKk3FGpIxzqkWkOQiaa5GmmYp4LsFQQTk1QIEzpnjGRuh6v7t19r01vkmqwmtTlqo2tvUJJSwkJJZx2KM3e1Q7670zebJ1RaVclxBIdvKSnbzkIK_Hrw7LbVqZ7Bf-sfX3etfa2Nb1Fvz_W9-spXb4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2135119895</pqid></control><display><type>article</type><title>Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching</title><source>ACS Publications</source><creator>Haydell, Michael W ; Centola, Mathias ; Adam, Volker ; Valero, Julián ; Famulok, Michael</creator><creatorcontrib>Haydell, Michael W ; Centola, Mathias ; Adam, Volker ; Valero, Julián ; Famulok, Michael</creatorcontrib><description>The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/jacs.8b08738</identifier><identifier>PMID: 30444607</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><ispartof>Journal of the American Chemical Society, 2018-12, Vol.140 (49), p.16868-16872</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a390t-a73ad2371bd4f34528ed431889f2c76c4072fc7bbda64f80e27242e020433a4d3</citedby><cites>FETCH-LOGICAL-a390t-a73ad2371bd4f34528ed431889f2c76c4072fc7bbda64f80e27242e020433a4d3</cites><orcidid>0000-0001-5878-6577 ; 0000-0002-5260-2770</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jacs.8b08738$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jacs.8b08738$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30444607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haydell, Michael W</creatorcontrib><creatorcontrib>Centola, Mathias</creatorcontrib><creatorcontrib>Adam, Volker</creatorcontrib><creatorcontrib>Valero, Julián</creatorcontrib><creatorcontrib>Famulok, Michael</creatorcontrib><title>Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices.</description><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNptkDtPwzAUhS0EoqWwMSOPDKRcPxI7Y1VelSqKUJkjx3HaVElc7AQUfj2pWmBhujrSd86VPoQuCYwJUHK7UdqPZQpSMHmEhiSkEISERsdoCAA0EDJiA3Tm_aaPnEpyigYMOOcRiCGaLU21tU6VWNUZfjUfxvkiLQ2e2rpxtsQ2xwrfPU--usrgtMML16ztytZ942VtG-s_i0avi3p1jk5yVXpzcbgj9PZwv5w-BfPF42w6mQeKxdAESjCVUSZImvGc8ZBKk3FGpIxzqkWkOQiaa5GmmYp4LsFQQTk1QIEzpnjGRuh6v7t19r01vkmqwmtTlqo2tvUJJSwkJJZx2KM3e1Q7670zebJ1RaVclxBIdvKSnbzkIK_Hrw7LbVqZ7Bf-sfX3etfa2Nb1Fvz_W9-spXb4</recordid><startdate>20181212</startdate><enddate>20181212</enddate><creator>Haydell, Michael W</creator><creator>Centola, Mathias</creator><creator>Adam, Volker</creator><creator>Valero, Julián</creator><creator>Famulok, Michael</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5878-6577</orcidid><orcidid>https://orcid.org/0000-0002-5260-2770</orcidid></search><sort><creationdate>20181212</creationdate><title>Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching</title><author>Haydell, Michael W ; Centola, Mathias ; Adam, Volker ; Valero, Julián ; Famulok, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a390t-a73ad2371bd4f34528ed431889f2c76c4072fc7bbda64f80e27242e020433a4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haydell, Michael W</creatorcontrib><creatorcontrib>Centola, Mathias</creatorcontrib><creatorcontrib>Adam, Volker</creatorcontrib><creatorcontrib>Valero, Julián</creatorcontrib><creatorcontrib>Famulok, Michael</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haydell, Michael W</au><au>Centola, Mathias</au><au>Adam, Volker</au><au>Valero, Julián</au><au>Famulok, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2018-12-12</date><risdate>2018</risdate><volume>140</volume><issue>49</issue><spage>16868</spage><epage>16872</epage><pages>16868-16872</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>The reversible switching of catalytic systems capable of performing complex DNA computing operations using the temporal control of two orthogonal photoswitches is described. Two distinct photoresponsive molecules have been separately incorporated into a split horseradish peroxidase-mimicking DNAzyme. We show that its catalytic function can be turned on and off reversibly upon irradiation with specific wavelengths of light. The system responds orthogonally to a selection of irradiation wavelengths and durations of irradiation. Furthermore, the DNAzyme exhibits reversible switching and retains this ability throughout multiple switching cycles. We apply our system as a light-controlled 4:2 multiplexer. Orthogonally photoswitchable DNAzyme-based catalysts as introduced here have potential use for controlling complex logical operations and for future applications in DNA nanodevices.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>30444607</pmid><doi>10.1021/jacs.8b08738</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-5878-6577</orcidid><orcidid>https://orcid.org/0000-0002-5260-2770</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0002-7863 |
ispartof | Journal of the American Chemical Society, 2018-12, Vol.140 (49), p.16868-16872 |
issn | 0002-7863 1520-5126 |
language | eng |
recordid | cdi_proquest_miscellaneous_2135119895 |
source | ACS Publications |
title | Temporal and Reversible Control of a DNAzyme by Orthogonal Photoswitching |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-11T08%3A32%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Temporal%20and%20Reversible%20Control%20of%20a%20DNAzyme%20by%20Orthogonal%20Photoswitching&rft.jtitle=Journal%20of%20the%20American%20Chemical%20Society&rft.au=Haydell,%20Michael%20W&rft.date=2018-12-12&rft.volume=140&rft.issue=49&rft.spage=16868&rft.epage=16872&rft.pages=16868-16872&rft.issn=0002-7863&rft.eissn=1520-5126&rft_id=info:doi/10.1021/jacs.8b08738&rft_dat=%3Cproquest_cross%3E2135119895%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2135119895&rft_id=info:pmid/30444607&rfr_iscdi=true |