Fluorescence Absorbance Inner-Filter Decomposition: The Role of Emission Shape on Estimates of Free Ca2+ Using Rhod-2

A method for decomposing complex emission spectra by correcting for known inner-filter effects is described. This approach builds on previous work using a linear combination of model emission spectra and combines the known absorption characteristics of the system to fit the composite emission spectr...

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Veröffentlicht in:	Applied spectroscopy 2007-02, Vol.61 (2), p.138-147
Hauptverfasser:	Territo, Paul R., Heil, Jeremy, Bose, Salil, Evans, Frank J., Balaban, Robert S.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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