Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B1, B2, G1 and G2

A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed d...

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Veröffentlicht in:	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 2018/10/25, Vol.59(5), pp.200-205
Hauptverfasser:	Yamasaki, Tomomi, Miyake, Shiro, Sato, Natsuki, Hirakawa, Yuki, Iwasa, Seiji, Narita, Hiroshi, Watanabe, Takaho
Format:	Artikel
Sprache:	eng
Schlagworte:	aflatoxin Aflatoxins ELISA Food analysis immunoassay Monoclonal antibodies monoclonal antibody Peanuts Performance assessment total aflatoxin
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container_title	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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creator	Yamasaki, Tomomi Miyake, Shiro Sato, Natsuki Hirakawa, Yuki Iwasa, Seiji Narita, Hiroshi Watanabe, Takaho
description	A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed dc-ELISA was 50–230 pg/mL for AFB1, 50–270 pg/mL for AFB2, 60–390 pg/mL for AFG1 and 65–700 pg/mL for AFG2. The recovery of AFs from spiked roasted peanuts was 98％. Further, when 4 samples actually contaminated with AFB1, AFB2, AFG1 and AFG2 were examined, the results of dc-ELISA were highly correlated with the values assigned by the Food Analysis Performance Assessment Scheme. The developed dc-ELISA appears to be suitable for the determination of total AFs at concentrations around the maximum permitted level (10 μg/kg for all foods) in Japan.
doi_str_mv	10.3358/shokueishi.59.200
format	Article
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The working range of the developed dc-ELISA was 50–230 pg/mL for AFB1, 50–270 pg/mL for AFB2, 60–390 pg/mL for AFG1 and 65–700 pg/mL for AFG2. The recovery of AFs from spiked roasted peanuts was 98％. Further, when 4 samples actually contaminated with AFB1, AFB2, AFG1 and AFG2 were examined, the results of dc-ELISA were highly correlated with the values assigned by the Food Analysis Performance Assessment Scheme. 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The developed dc-ELISA appears to be suitable for the determination of total AFs at concentrations around the maximum permitted level (10 μg/kg for all foods) in Japan.</description><subject>aflatoxin</subject><subject>Aflatoxins</subject><subject>ELISA</subject><subject>Food analysis</subject><subject>immunoassay</subject><subject>Monoclonal antibodies</subject><subject>monoclonal antibody</subject><subject>Peanuts</subject><subject>Performance assessment</subject><subject>total aflatoxin</subject><issn>0015-6426</issn><issn>1882-1006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNpdkU2P0zAQhi0EEqXwA7hZ4sJhU_xRJ-mx-0FZqQgJ9W5NnDF1N7GL7ewS_gn_llRdLWJPI837PCONXkLec7aQUtWf0j7cDejS3i3UaiEYe0FmvK5FwRkrX5IZY1wV5VKUr8mblA7TkklWz8ifa7zHLhx79JkGS2_877HHYuv8Hbb0tu8HH1KIzSlepwQjtSHStYduTC6djF3I0NG17SCHX84neglpUoOnX4MPpgv-FPvsmtCO9DuCye4e6YPL-_8sfkEvxQXdcAq-pRvxlryy0CV89zjnZPf5Znf1pdh-29xerbeFkazMRdUsG1SrxtTMKmibGgxURrXStlIpwEa2lbSsKoVkQkpAsxLMgoRGlbC0ck4-ns8eY_g5YMq6d8lg14HHMCQtuJS1UHwac_LhGXoIQ5zemyghVVmVTKmJ4mfKxJBSRKuP0fUQR82ZPnWl_3Wl1UpPXU3O9dk5pAw_8MmAmJ3p8JmhHrWn2OwhavTyL5LjpQg</recordid><startdate>20181025</startdate><enddate>20181025</enddate><creator>Yamasaki, Tomomi</creator><creator>Miyake, Shiro</creator><creator>Sato, Natsuki</creator><creator>Hirakawa, Yuki</creator><creator>Iwasa, Seiji</creator><creator>Narita, Hiroshi</creator><creator>Watanabe, Takaho</creator><general>Japanese Society for Food Hygiene and Safety</general><general>Japan Science and Technology Agency</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H96</scope><scope>H97</scope><scope>H98</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20181025</creationdate><title>Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B1, B2, G1 and G2</title><author>Yamasaki, Tomomi ; 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subjects	aflatoxin Aflatoxins ELISA Food analysis immunoassay Monoclonal antibodies monoclonal antibody Peanuts Performance assessment total aflatoxin
title	Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B1, B2, G1 and G2
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