Differentiation of Laboratory Strains of Red Flour Beetle Tribolium castaneum Collected from Various Areas
We recently reported polymorphism in the mitochondrial genes of Tribolium castaneum, a stored grain pest. Here, we show that T. castaneum samples from the main island of Japan can be easily differentiated from samples from other areas by using real-time PCR with minor groove binding (MGB) TaqMan pro...
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Veröffentlicht in: | Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 2018/10/25, Vol.59(5), pp.220-222 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | We recently reported polymorphism in the mitochondrial genes of Tribolium castaneum, a stored grain pest. Here, we show that T. castaneum samples from the main island of Japan can be easily differentiated from samples from other areas by using real-time PCR with minor groove binding (MGB) TaqMan probes containing a single nucleotide polymorphism (SNP) to amplify a 179-bp fragment. This assay was also applicable to samples stored at room temperature for 5 months after natural death, and samples whose DNA was artificially degraded by heat, pressure, or gamma-ray irradiation. |
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ISSN: | 0015-6426 1882-1006 |
DOI: | 10.3358/shokueishi.59.220 |