Differentiation of Laboratory Strains of Red Flour Beetle Tribolium castaneum Collected from Various Areas

We recently reported polymorphism in the mitochondrial genes of Tribolium castaneum, a stored grain pest. Here, we show that T. castaneum samples from the main island of Japan can be easily differentiated from samples from other areas by using real-time PCR with minor groove binding (MGB) TaqMan pro...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 2018/10/25, Vol.59(5), pp.220-222
Hauptverfasser: Yamauchi, Hiromasa, Harada, Masami, Miyanoshita, Akihiro
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:We recently reported polymorphism in the mitochondrial genes of Tribolium castaneum, a stored grain pest. Here, we show that T. castaneum samples from the main island of Japan can be easily differentiated from samples from other areas by using real-time PCR with minor groove binding (MGB) TaqMan probes containing a single nucleotide polymorphism (SNP) to amplify a 179-bp fragment. This assay was also applicable to samples stored at room temperature for 5 months after natural death, and samples whose DNA was artificially degraded by heat, pressure, or gamma-ray irradiation.
ISSN:0015-6426
1882-1006
DOI:10.3358/shokueishi.59.220