Purification and properties of recombinant DNA methyltransferase M2.BstSE of the BstSEI nickase-modification system

The operon for the Bacillus stearothermophilus SE-589 nickase-modification system (NM.BstSEI, recognition site 5'-GAGTC-3') includes two DNA methyltransferase (M.) genes, bstSEIM1 and bstSEIM2. The gene encoding M2.BstSEI was cloned in pJW and expressed in Escherichia coli cells. M2.BstSEI...

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Veröffentlicht in:	Molecular biology (New York) 2009-02, Vol.43 (1), p.8-15
Hauptverfasser:	Chernukhin, V. A, Seggewiss, J, Kashirina, Yu. G, Gonchar, D. A, Degtyarev, S. Kh
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus stearothermophilus Biochemistry Biomedical and Life Sciences Deoxyribonucleic acid DNA E coli Enzymes Escherichia coli Genes Genomics. Transcriptomics. Proteomics Human Genetics Kinetics Life Sciences Molecular biology
Online-Zugang:	Volltext
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