Definition of phosphoinositide distribution in the nanoscale
New functionalities of phosphoinositides (PIs) are being revealed continuously, and the scale of the membrane area studied is becoming smaller, from the micrometer range like the entire surface of organelles to the nanometer range as in subdomains of organelles. Concurrently, function of less abunda...
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| Veröffentlicht in: | Current opinion in cell biology 2019-04, Vol.57, p.33-39 |
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| container_title | Current opinion in cell biology |
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| creator | Tsuji, Takuma Takatori, Sho Fujimoto, Toyoshi |
| description | New functionalities of phosphoinositides (PIs) are being revealed continuously, and the scale of the membrane area studied is becoming smaller, from the micrometer range like the entire surface of organelles to the nanometer range as in subdomains of organelles. Concurrently, function of less abundant PIs, such as PI(3,4)P2 and PI(3,5)P2, attracts increasing attention. In accordance with the progress, finer and more accurate information on PI distribution is required. The fluorescence biosensor method utilizing PI-binding domains and/or immunolabeling with anti-PI antibodies are used for this purpose in most studies but both methods are known to have caveats. In this article, we examined how PI distribution was defined in recent studies and discussed whether methodological uncertainty has any bearing on the results. |
| doi_str_mv | 10.1016/j.ceb.2018.10.008 |
| format | Article |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological Transport Biosensing Techniques Cell Membrane - chemistry Cilia - chemistry Fluorescence Immunohistochemistry Organelles - chemistry Phosphatidylinositols - analysis |
| title | Definition of phosphoinositide distribution in the nanoscale |
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