The role of thioredoxin reductase in gold nanoparticle radiosensitization effects
To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs). A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays. GNP incubation led to a time-dependent mitochondria membrane de...
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| Veröffentlicht in: | Nanomedicine (London, England) England), 2018-11, Vol.13 (22), p.2917-2937 |
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| container_title | Nanomedicine (London, England) |
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| creator | Penninckx, Sébastien Heuskin, Anne-Catherine Michiels, Carine Lucas, Stéphane |
| description | To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).
A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.
GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.
We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy. |
| doi_str_mv | 10.2217/nnm-2018-0171 |
| format | Article |
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A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.
GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.
We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.</description><identifier>ISSN: 1743-5889</identifier><identifier>EISSN: 1748-6963</identifier><identifier>DOI: 10.2217/nnm-2018-0171</identifier><identifier>PMID: 30427254</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Apoptosis ; Autophagy ; biological mechanism ; Cancer therapies ; Copper ; gold nanoparticles ; Lasers ; Leukemia ; Lung cancer ; Microscopy ; Nanoparticles ; proton irradiation ; Radiation therapy ; radiosensitization ; thioredoxin reductase ; X-rays</subject><ispartof>Nanomedicine (London, England), 2018-11, Vol.13 (22), p.2917-2937</ispartof><rights>2018 Future Medicine Ltd</rights><rights>Copyright Future Medicine Ltd Nov 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-ef895d05eaff5ea917d0549a245e247b8899dfcd4f7fa76c772d4542dbcd40b23</citedby><cites>FETCH-LOGICAL-c371t-ef895d05eaff5ea917d0549a245e247b8899dfcd4f7fa76c772d4542dbcd40b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30427254$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Penninckx, Sébastien</creatorcontrib><creatorcontrib>Heuskin, Anne-Catherine</creatorcontrib><creatorcontrib>Michiels, Carine</creatorcontrib><creatorcontrib>Lucas, Stéphane</creatorcontrib><title>The role of thioredoxin reductase in gold nanoparticle radiosensitization effects</title><title>Nanomedicine (London, England)</title><addtitle>Nanomedicine (Lond)</addtitle><description>To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).
A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.
GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.
We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.</description><subject>Apoptosis</subject><subject>Autophagy</subject><subject>biological mechanism</subject><subject>Cancer therapies</subject><subject>Copper</subject><subject>gold nanoparticles</subject><subject>Lasers</subject><subject>Leukemia</subject><subject>Lung cancer</subject><subject>Microscopy</subject><subject>Nanoparticles</subject><subject>proton irradiation</subject><subject>Radiation therapy</subject><subject>radiosensitization</subject><subject>thioredoxin reductase</subject><subject>X-rays</subject><issn>1743-5889</issn><issn>1748-6963</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kEtLAzEURoMoWh9LtzLgxs1oXjOZWUrxBYIIdR3S5MZGpklNMqD-elOrLgQ3ufnCycflIHRM8DmlRFx4v6wpJl2NiSBbaEIE7-q2b9n2153VTdf1e2g_pReMm44SvIv2GOZU0IZP0ONsAVUMA1TBVnnhQgQT3pyvyhx1VgmqEp7DYCqvfFipmJ0udFTGhQQ-uew-VHbBV2At6JwO0Y5VQ4Kj73mAnq6vZtPb-v7h5m56eV9rJkiuwXZ9Y3ADytpy9ESUwHtFeQOUi3nZujdWG26FVaLVQlDDG07NvLzhOWUH6GzTu4rhdYSU5dIlDcOgPIQxSUoY46wVLSvo6R_0JYzRl-0kZZS0gna8KVS9oXQMKUWwchXdUsV3SbBcu5bFtVy7lmvXhT_5bh3nSzC_9I_cAvQbwI55jJC0A69BblL54bTz8E_5J4Jzjn0</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Penninckx, Sébastien</creator><creator>Heuskin, Anne-Catherine</creator><creator>Michiels, Carine</creator><creator>Lucas, Stéphane</creator><general>Future Medicine Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20181101</creationdate><title>The role of thioredoxin reductase in gold nanoparticle radiosensitization effects</title><author>Penninckx, Sébastien ; Heuskin, Anne-Catherine ; Michiels, Carine ; Lucas, Stéphane</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-ef895d05eaff5ea917d0549a245e247b8899dfcd4f7fa76c772d4542dbcd40b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Apoptosis</topic><topic>Autophagy</topic><topic>biological mechanism</topic><topic>Cancer therapies</topic><topic>Copper</topic><topic>gold nanoparticles</topic><topic>Lasers</topic><topic>Leukemia</topic><topic>Lung cancer</topic><topic>Microscopy</topic><topic>Nanoparticles</topic><topic>proton irradiation</topic><topic>Radiation therapy</topic><topic>radiosensitization</topic><topic>thioredoxin reductase</topic><topic>X-rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Penninckx, Sébastien</creatorcontrib><creatorcontrib>Heuskin, Anne-Catherine</creatorcontrib><creatorcontrib>Michiels, Carine</creatorcontrib><creatorcontrib>Lucas, Stéphane</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Nanomedicine (London, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Penninckx, Sébastien</au><au>Heuskin, Anne-Catherine</au><au>Michiels, Carine</au><au>Lucas, Stéphane</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of thioredoxin reductase in gold nanoparticle radiosensitization effects</atitle><jtitle>Nanomedicine (London, England)</jtitle><addtitle>Nanomedicine (Lond)</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>13</volume><issue>22</issue><spage>2917</spage><epage>2937</epage><pages>2917-2937</pages><issn>1743-5889</issn><eissn>1748-6963</eissn><abstract>To identify new mechanisms responsible for the radiosensitization effect of gold nanoparticles (GNPs).
A549 lung carcinoma cells were incubated with 10-nm GNPs during 6 or 24 h before to be exposed to 25 keV/μm protons or 225 kV x-rays.
GNP incubation led to a time-dependent mitochondria membrane depolarization, oxidative stress and to x-ray and proton radiosensitization. Moreover, a marked inhibition of thioredoxin reductase was observed. Irradiation of cells invalidated for thioredoxin reductase evidenced a radiosensitization effect, suggesting that this enzyme is a potential GNP target.
We suggest that GNPs play a radiosensitizer role by weakening detoxification systems. Altogether, these results open up promising novel strategies for the development of nanotechnologies associated to radiotherapy.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>30427254</pmid><doi>10.2217/nnm-2018-0171</doi><tpages>21</tpages></addata></record> |
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| issn | 1743-5889 1748-6963 |
| language | eng |
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| source | PubMed Central |
| subjects | Apoptosis Autophagy biological mechanism Cancer therapies Copper gold nanoparticles Lasers Leukemia Lung cancer Microscopy Nanoparticles proton irradiation Radiation therapy radiosensitization thioredoxin reductase X-rays |
| title | The role of thioredoxin reductase in gold nanoparticle radiosensitization effects |
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