Selective Enrichment of Slow-Growing Bacteria in a Metabolism-Wide CRISPRi Library with a TIMER Protein

Construction of pooled genetic variant libraries has become very fast and versatile. The current limitation of this technique is to select cells with a desired phenotype from very large libraries. Especially cells with poor fitness and slow growth are difficult to select because they are rapidly out...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	ACS synthetic biology 2018-12, Vol.7 (12), p.2775-2782
Hauptverfasser:	Beuter, Dominik, Gomes-Filho, José Vicente, Randau, Lennart, Díaz-Pascual, Francisco, Drescher, Knut, Link, Hannes
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!