Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations
Purpose The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy. Methods The study group included 270 embryos that underwent biopsies on day 3 fo...
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| Veröffentlicht in: | Journal of assisted reproduction and genetics 2019-02, Vol.36 (2), p.315-324 |
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| container_title | Journal of assisted reproduction and genetics |
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| creator | Amir, Hadar Barbash-Hazan, Shiri Kalma, Yael Frumkin, Tsvia Malcov, Mira Samara, Nivin Hasson, Joseph Reches, Adi Azem, Foad Ben-Yosef, Dalit |
| description | Purpose
The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy.
Methods
The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. The timing of these events was compared between balanced and unbalanced embryos, potentially viable and nonviable variants, and maternal versus paternal inheritance of the translocation.
Results
The PGD analysis found that 209 (77%) of the 270 biopsied embryos carried an unbalanced translocation. Embryos carrying unbalanced translocations, which are expected to lead to implantation failure or miscarriage, cleaved less synchronously and were delayed in time of cleavage to the 4-cell stage (t4) and in time of start of blastulation (tSB) compared with balanced embryos (
P
|
| doi_str_mv | 10.1007/s10815-018-1361-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_2132737303</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2132124460</sourcerecordid><originalsourceid>FETCH-LOGICAL-p212t-b32c24419ba927cd7315b51fb386e61b2152c6f3cc2c655f3a058d18228f7b563</originalsourceid><addsrcrecordid>eNpdkc1LxDAQxYMo7vrxB3iRghcv0UymabNHWfyCBS96DkmarpW2qUm7sP-9WVcRPL2B-fF4M4-QC2A3wFh5G4FJEJSBpIAFUHlA5iBKpCUiO0wzE5KyvJAzchLjB2NsITkekxmynAPmOCfvr03naKuH6LKm0-umX2fBbZxuY1a5Vm9dlXTjWj90rh8zX2euM2HrY2Z1CNsdP_VGt7q3CbXvwXc--k632Rh0H1tv9dj4Pp6RozqZuvMfPSVvD_evyye6enl8Xt6t6MCBj9QgtzzPYWH0gpe2KhGEEVAblIUrwHAQ3BY1WptEiBp1urECybmsSyMKPCXXe98h-M_JxVF1TbSuTQGdn6JKd_MSS2SY0Kt_6IefQp_SfVOQchQsUZc_1GQ6V6khpD-Frfr9YQL4Hohp1a9d-LMBpnZFqX1RKhWldkUpiV8rZ4PV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2132124460</pqid></control><display><type>article</type><title>Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Amir, Hadar ; Barbash-Hazan, Shiri ; Kalma, Yael ; Frumkin, Tsvia ; Malcov, Mira ; Samara, Nivin ; Hasson, Joseph ; Reches, Adi ; Azem, Foad ; Ben-Yosef, Dalit</creator><creatorcontrib>Amir, Hadar ; Barbash-Hazan, Shiri ; Kalma, Yael ; Frumkin, Tsvia ; Malcov, Mira ; Samara, Nivin ; Hasson, Joseph ; Reches, Adi ; Azem, Foad ; Ben-Yosef, Dalit</creatorcontrib><description>Purpose
The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy.
Methods
The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. The timing of these events was compared between balanced and unbalanced embryos, potentially viable and nonviable variants, and maternal versus paternal inheritance of the translocation.
Results
The PGD analysis found that 209 (77%) of the 270 biopsied embryos carried an unbalanced translocation. Embryos carrying unbalanced translocations, which are expected to lead to implantation failure or miscarriage, cleaved less synchronously and were delayed in time of cleavage to the 4-cell stage (t4) and in time of start of blastulation (tSB) compared with balanced embryos (
P
< 0.05). Furthermore, embryos carrying nonviable translocations demonstrated a significant delay at the time of pronuclei fading (tPNf) compared with those carrying potentially viable translocations (
P
< 0.05). Embryos whose unbalanced translocations were of maternal origin were significantly delayed in most of the morphokinetic parameters (including tPNf, t2, t3, t4, t6, t7, t8, cc2, s2, and tSB) compared with embryos carrying balanced translocations (
P
< 0.05).
Conclusions
Embryos carrying unbalanced chromosomal translocations mainly of maternal origin undergo delayed development and asynchronous cleavage that may lead to implantation failure or miscarriage.</description><identifier>ISSN: 1058-0468</identifier><identifier>EISSN: 1573-7330</identifier><identifier>DOI: 10.1007/s10815-018-1361-8</identifier><identifier>PMID: 30421343</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Abortion, Spontaneous - epidemiology ; Abortion, Spontaneous - pathology ; Blastocyst - metabolism ; Blastocyst - pathology ; Chromosome translocations ; Embryo Biology ; Embryo Culture Techniques ; Embryo Implantation - genetics ; Embryo Transfer - methods ; Embryonic Development - genetics ; Embryos ; Female ; Fertilization in Vitro ; Genetic screening ; Gynecology ; Human Genetics ; Humans ; Male ; Medicine ; Medicine & Public Health ; Microscopy ; Miscarriage ; Pregnancy ; Preimplantation Diagnosis ; Reproductive Medicine ; Sperm Injections, Intracytoplasmic - methods ; Translocation, Genetic - genetics</subject><ispartof>Journal of assisted reproduction and genetics, 2019-02, Vol.36 (2), p.315-324</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Journal of Assisted Reproduction and Genetics is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-p212t-b32c24419ba927cd7315b51fb386e61b2152c6f3cc2c655f3a058d18228f7b563</cites><orcidid>0000-0001-9987-8697</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10815-018-1361-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10815-018-1361-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30421343$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amir, Hadar</creatorcontrib><creatorcontrib>Barbash-Hazan, Shiri</creatorcontrib><creatorcontrib>Kalma, Yael</creatorcontrib><creatorcontrib>Frumkin, Tsvia</creatorcontrib><creatorcontrib>Malcov, Mira</creatorcontrib><creatorcontrib>Samara, Nivin</creatorcontrib><creatorcontrib>Hasson, Joseph</creatorcontrib><creatorcontrib>Reches, Adi</creatorcontrib><creatorcontrib>Azem, Foad</creatorcontrib><creatorcontrib>Ben-Yosef, Dalit</creatorcontrib><title>Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations</title><title>Journal of assisted reproduction and genetics</title><addtitle>J Assist Reprod Genet</addtitle><addtitle>J Assist Reprod Genet</addtitle><description>Purpose
The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy.
Methods
The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. The timing of these events was compared between balanced and unbalanced embryos, potentially viable and nonviable variants, and maternal versus paternal inheritance of the translocation.
Results
The PGD analysis found that 209 (77%) of the 270 biopsied embryos carried an unbalanced translocation. Embryos carrying unbalanced translocations, which are expected to lead to implantation failure or miscarriage, cleaved less synchronously and were delayed in time of cleavage to the 4-cell stage (t4) and in time of start of blastulation (tSB) compared with balanced embryos (
P
< 0.05). Furthermore, embryos carrying nonviable translocations demonstrated a significant delay at the time of pronuclei fading (tPNf) compared with those carrying potentially viable translocations (
P
< 0.05). Embryos whose unbalanced translocations were of maternal origin were significantly delayed in most of the morphokinetic parameters (including tPNf, t2, t3, t4, t6, t7, t8, cc2, s2, and tSB) compared with embryos carrying balanced translocations (
P
< 0.05).
Conclusions
Embryos carrying unbalanced chromosomal translocations mainly of maternal origin undergo delayed development and asynchronous cleavage that may lead to implantation failure or miscarriage.</description><subject>Abortion, Spontaneous - epidemiology</subject><subject>Abortion, Spontaneous - pathology</subject><subject>Blastocyst - metabolism</subject><subject>Blastocyst - pathology</subject><subject>Chromosome translocations</subject><subject>Embryo Biology</subject><subject>Embryo Culture Techniques</subject><subject>Embryo Implantation - genetics</subject><subject>Embryo Transfer - methods</subject><subject>Embryonic Development - genetics</subject><subject>Embryos</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Genetic screening</subject><subject>Gynecology</subject><subject>Human Genetics</subject><subject>Humans</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy</subject><subject>Miscarriage</subject><subject>Pregnancy</subject><subject>Preimplantation Diagnosis</subject><subject>Reproductive Medicine</subject><subject>Sperm Injections, Intracytoplasmic - methods</subject><subject>Translocation, Genetic - genetics</subject><issn>1058-0468</issn><issn>1573-7330</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkc1LxDAQxYMo7vrxB3iRghcv0UymabNHWfyCBS96DkmarpW2qUm7sP-9WVcRPL2B-fF4M4-QC2A3wFh5G4FJEJSBpIAFUHlA5iBKpCUiO0wzE5KyvJAzchLjB2NsITkekxmynAPmOCfvr03naKuH6LKm0-umX2fBbZxuY1a5Vm9dlXTjWj90rh8zX2euM2HrY2Z1CNsdP_VGt7q3CbXvwXc--k632Rh0H1tv9dj4Pp6RozqZuvMfPSVvD_evyye6enl8Xt6t6MCBj9QgtzzPYWH0gpe2KhGEEVAblIUrwHAQ3BY1WptEiBp1urECybmsSyMKPCXXe98h-M_JxVF1TbSuTQGdn6JKd_MSS2SY0Kt_6IefQp_SfVOQchQsUZc_1GQ6V6khpD-Frfr9YQL4Hohp1a9d-LMBpnZFqX1RKhWldkUpiV8rZ4PV</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Amir, Hadar</creator><creator>Barbash-Hazan, Shiri</creator><creator>Kalma, Yael</creator><creator>Frumkin, Tsvia</creator><creator>Malcov, Mira</creator><creator>Samara, Nivin</creator><creator>Hasson, Joseph</creator><creator>Reches, Adi</creator><creator>Azem, Foad</creator><creator>Ben-Yosef, Dalit</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9987-8697</orcidid></search><sort><creationdate>20190201</creationdate><title>Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations</title><author>Amir, Hadar ; Barbash-Hazan, Shiri ; Kalma, Yael ; Frumkin, Tsvia ; Malcov, Mira ; Samara, Nivin ; Hasson, Joseph ; Reches, Adi ; Azem, Foad ; Ben-Yosef, Dalit</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p212t-b32c24419ba927cd7315b51fb386e61b2152c6f3cc2c655f3a058d18228f7b563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abortion, Spontaneous - epidemiology</topic><topic>Abortion, Spontaneous - pathology</topic><topic>Blastocyst - metabolism</topic><topic>Blastocyst - pathology</topic><topic>Chromosome translocations</topic><topic>Embryo Biology</topic><topic>Embryo Culture Techniques</topic><topic>Embryo Implantation - genetics</topic><topic>Embryo Transfer - methods</topic><topic>Embryonic Development - genetics</topic><topic>Embryos</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Genetic screening</topic><topic>Gynecology</topic><topic>Human Genetics</topic><topic>Humans</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy</topic><topic>Miscarriage</topic><topic>Pregnancy</topic><topic>Preimplantation Diagnosis</topic><topic>Reproductive Medicine</topic><topic>Sperm Injections, Intracytoplasmic - methods</topic><topic>Translocation, Genetic - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amir, Hadar</creatorcontrib><creatorcontrib>Barbash-Hazan, Shiri</creatorcontrib><creatorcontrib>Kalma, Yael</creatorcontrib><creatorcontrib>Frumkin, Tsvia</creatorcontrib><creatorcontrib>Malcov, Mira</creatorcontrib><creatorcontrib>Samara, Nivin</creatorcontrib><creatorcontrib>Hasson, Joseph</creatorcontrib><creatorcontrib>Reches, Adi</creatorcontrib><creatorcontrib>Azem, Foad</creatorcontrib><creatorcontrib>Ben-Yosef, Dalit</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of assisted reproduction and genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amir, Hadar</au><au>Barbash-Hazan, Shiri</au><au>Kalma, Yael</au><au>Frumkin, Tsvia</au><au>Malcov, Mira</au><au>Samara, Nivin</au><au>Hasson, Joseph</au><au>Reches, Adi</au><au>Azem, Foad</au><au>Ben-Yosef, Dalit</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations</atitle><jtitle>Journal of assisted reproduction and genetics</jtitle><stitle>J Assist Reprod Genet</stitle><addtitle>J Assist Reprod Genet</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>36</volume><issue>2</issue><spage>315</spage><epage>324</epage><pages>315-324</pages><issn>1058-0468</issn><eissn>1573-7330</eissn><abstract>Purpose
The purpose of the study was to compare the morphokinetic parameters of embryos carrying balanced chromosomal translocations with those carrying unbalanced chromosomal translocations using time-lapse microscopy.
Methods
The study group included 270 embryos that underwent biopsies on day 3 for preimplantation genetic diagnosis (PGD) for chromosomal translocations in our unit between 2013 and 2015. All embryos were incubated under time-lapse microscopy and evaluated for timing of developmental events up to day 5. The timing of these events was compared between balanced and unbalanced embryos, potentially viable and nonviable variants, and maternal versus paternal inheritance of the translocation.
Results
The PGD analysis found that 209 (77%) of the 270 biopsied embryos carried an unbalanced translocation. Embryos carrying unbalanced translocations, which are expected to lead to implantation failure or miscarriage, cleaved less synchronously and were delayed in time of cleavage to the 4-cell stage (t4) and in time of start of blastulation (tSB) compared with balanced embryos (
P
< 0.05). Furthermore, embryos carrying nonviable translocations demonstrated a significant delay at the time of pronuclei fading (tPNf) compared with those carrying potentially viable translocations (
P
< 0.05). Embryos whose unbalanced translocations were of maternal origin were significantly delayed in most of the morphokinetic parameters (including tPNf, t2, t3, t4, t6, t7, t8, cc2, s2, and tSB) compared with embryos carrying balanced translocations (
P
< 0.05).
Conclusions
Embryos carrying unbalanced chromosomal translocations mainly of maternal origin undergo delayed development and asynchronous cleavage that may lead to implantation failure or miscarriage.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30421343</pmid><doi>10.1007/s10815-018-1361-8</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-9987-8697</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of assisted reproduction and genetics, 2019-02, Vol.36 (2), p.315-324 |
| issn | 1058-0468 1573-7330 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Abortion, Spontaneous - epidemiology Abortion, Spontaneous - pathology Blastocyst - metabolism Blastocyst - pathology Chromosome translocations Embryo Biology Embryo Culture Techniques Embryo Implantation - genetics Embryo Transfer - methods Embryonic Development - genetics Embryos Female Fertilization in Vitro Genetic screening Gynecology Human Genetics Humans Male Medicine Medicine & Public Health Microscopy Miscarriage Pregnancy Preimplantation Diagnosis Reproductive Medicine Sperm Injections, Intracytoplasmic - methods Translocation, Genetic - genetics |
| title | Time-lapse imaging reveals delayed development of embryos carrying unbalanced chromosomal translocations |
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