Genetic and morphometric categorization of Taenia ovis from Sheep in Iran
Little is known about the genetic and morphological characters of Taenia ovis. The purpose of the present study was to characterize sheep isolates of T. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens of Cysticercus ovis were collected f...
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| Veröffentlicht in: | Parasitology 2019-04, Vol.146 (5), p.563-568 |
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| creator | Rostami, Sima Salavati, Reza Beech, Robin N. Babaei, Zahra Sharbatkhori, Mitra Shamsaddini, Saeedeh Nasibi, Saeid Harandi, Majid Fasihi |
| description | Little is known about the genetic and morphological characters of Taenia ovis. The purpose of the present study was to characterize sheep isolates of T. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens of Cysticercus ovis were collected from 18 slaughterhouses in Iran. The mean ± s.d. for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4 µm, respectively. CO1 and 12S rRNA sequence analysis showed 11 and nine haplotypes, respectively. The level of pairwise nucleotide variations between individual haplotypes of CO1 and 12S rRNA genes were 0.3–1.1 and 0.2–1.0%, respectively. Level of nucleotide variation in CO1 and 12S rRNA between T. ovis haplotypes from present study and eight other Taenia species was found to be 11.3–17.8 and 5.3–16.3%, respectively. Phylogenetic analysis clustered all T. ovis isolates into a single clade comprised of the all CO1 and 12S rRNA haplotypes. CO1 nucleotide difference between T. ovis ovis and T. asiatica was 13.6% that is lesser than the corresponding difference between T. ovis ovis and T. ovis krabbei, warranting the designation of two separate species as T. ovis and T. krabbei. Interclass correlation coefficients showed that there was no significant association between rostellar hook length variation and the variability of the mitochondrial genes. |
| doi_str_mv | 10.1017/S0031182018001907 |
| format | Article |
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The purpose of the present study was to characterize sheep isolates of T. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens of Cysticercus ovis were collected from 18 slaughterhouses in Iran. The mean ± s.d. for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4 µm, respectively. CO1 and 12S rRNA sequence analysis showed 11 and nine haplotypes, respectively. The level of pairwise nucleotide variations between individual haplotypes of CO1 and 12S rRNA genes were 0.3–1.1 and 0.2–1.0%, respectively. Level of nucleotide variation in CO1 and 12S rRNA between T. ovis haplotypes from present study and eight other Taenia species was found to be 11.3–17.8 and 5.3–16.3%, respectively. Phylogenetic analysis clustered all T. ovis isolates into a single clade comprised of the all CO1 and 12S rRNA haplotypes. CO1 nucleotide difference between T. ovis ovis and T. asiatica was 13.6% that is lesser than the corresponding difference between T. ovis ovis and T. ovis krabbei, warranting the designation of two separate species as T. ovis and T. krabbei. Interclass correlation coefficients showed that there was no significant association between rostellar hook length variation and the variability of the mitochondrial genes.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182018001907</identifier><identifier>PMID: 30419971</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Abattoirs ; Animals ; Correlation coefficient ; Correlation coefficients ; Electron Transport Complex IV - analysis ; Genes ; Genetic diversity ; Genetic Variation ; Haplotypes ; Helminth Proteins - analysis ; Hooks ; Iran ; Larva - anatomy & histology ; Mitochondria ; Mitochondrial DNA ; Mitochondrial Proteins - analysis ; Morphometry ; Mutation ; Nucleotides ; Parasites ; Phylogeny ; RNA, Helminth - analysis ; RNA, Ribosomal - analysis ; rRNA 12S ; Sequence analysis ; Sheep ; Sheep Diseases - parasitology ; Software ; Taenia - anatomy & histology ; Taenia - genetics ; Taenia - growth & development ; Taenia asiatica ; Taenia ovis ; Taenia ovis krabbei ; Taenia ovis ovis ; Taeniasis - parasitology ; Taeniasis - veterinary ; Vaccines ; Worms</subject><ispartof>Parasitology, 2019-04, Vol.146 (5), p.563-568</ispartof><rights>Copyright © Cambridge University Press 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-399272e1c6efaf85bc7363c30685fcd4e2295832de5cfa00f3ed43bbb435fa343</citedby><cites>FETCH-LOGICAL-c373t-399272e1c6efaf85bc7363c30685fcd4e2295832de5cfa00f3ed43bbb435fa343</cites><orcidid>0000-0003-3257-5389</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182018001907/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,315,782,786,27931,27932,55635</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30419971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rostami, Sima</creatorcontrib><creatorcontrib>Salavati, Reza</creatorcontrib><creatorcontrib>Beech, Robin N.</creatorcontrib><creatorcontrib>Babaei, Zahra</creatorcontrib><creatorcontrib>Sharbatkhori, Mitra</creatorcontrib><creatorcontrib>Shamsaddini, Saeedeh</creatorcontrib><creatorcontrib>Nasibi, Saeid</creatorcontrib><creatorcontrib>Harandi, Majid Fasihi</creatorcontrib><title>Genetic and morphometric categorization of Taenia ovis from Sheep in Iran</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Little is known about the genetic and morphological characters of Taenia ovis. The purpose of the present study was to characterize sheep isolates of T. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens of Cysticercus ovis were collected from 18 slaughterhouses in Iran. The mean ± s.d. for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4 µm, respectively. CO1 and 12S rRNA sequence analysis showed 11 and nine haplotypes, respectively. The level of pairwise nucleotide variations between individual haplotypes of CO1 and 12S rRNA genes were 0.3–1.1 and 0.2–1.0%, respectively. Level of nucleotide variation in CO1 and 12S rRNA between T. ovis haplotypes from present study and eight other Taenia species was found to be 11.3–17.8 and 5.3–16.3%, respectively. Phylogenetic analysis clustered all T. ovis isolates into a single clade comprised of the all CO1 and 12S rRNA haplotypes. CO1 nucleotide difference between T. ovis ovis and T. asiatica was 13.6% that is lesser than the corresponding difference between T. ovis ovis and T. ovis krabbei, warranting the designation of two separate species as T. ovis and T. krabbei. Interclass correlation coefficients showed that there was no significant association between rostellar hook length variation and the variability of the mitochondrial genes.</description><subject>Abattoirs</subject><subject>Animals</subject><subject>Correlation coefficient</subject><subject>Correlation coefficients</subject><subject>Electron Transport Complex IV - analysis</subject><subject>Genes</subject><subject>Genetic diversity</subject><subject>Genetic Variation</subject><subject>Haplotypes</subject><subject>Helminth Proteins - analysis</subject><subject>Hooks</subject><subject>Iran</subject><subject>Larva - anatomy & histology</subject><subject>Mitochondria</subject><subject>Mitochondrial DNA</subject><subject>Mitochondrial Proteins - analysis</subject><subject>Morphometry</subject><subject>Mutation</subject><subject>Nucleotides</subject><subject>Parasites</subject><subject>Phylogeny</subject><subject>RNA, Helminth - analysis</subject><subject>RNA, Ribosomal - analysis</subject><subject>rRNA 12S</subject><subject>Sequence analysis</subject><subject>Sheep</subject><subject>Sheep Diseases - parasitology</subject><subject>Software</subject><subject>Taenia - anatomy & histology</subject><subject>Taenia - genetics</subject><subject>Taenia - growth & development</subject><subject>Taenia asiatica</subject><subject>Taenia ovis</subject><subject>Taenia ovis krabbei</subject><subject>Taenia ovis ovis</subject><subject>Taeniasis - parasitology</subject><subject>Taeniasis - veterinary</subject><subject>Vaccines</subject><subject>Worms</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kEFLw0AQhRdRbK3-AC-y4MVLdHcn2SRHKVoLBQ-t57DZzLZbmmzdTQT99aa0KiieBma-997wCLnk7JYznt7NGQPOM8F4xhjPWXpEhjyWeZRxyY_JcHeOdvcBOQthzRiTIMUpGQCLeZ6nfEimE2ywtZqqpqK189uVq7H1_UKrFpfO2w_VWtdQZ-hCYWMVdW82UONdTecrxC21DZ161ZyTE6M2AS8Oc0ReHh8W46do9jyZju9nkYYU2gjyXKQCuZZolMmSUqcgQQOTWWJ0FaMQeZKBqDDRRjFmAKsYyrKMITEKYhiRm73v1rvXDkNb1DZo3GxUg64LheDQB8gkTnr0-he6dp1v-u8KkaSplFlP9hTfU9q7EDyaYuttrfx7wVmx67n403OvuTo4d2WN1bfiq9gegIOpqktvqyX-ZP9v-wkgjYXI</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Rostami, Sima</creator><creator>Salavati, Reza</creator><creator>Beech, Robin N.</creator><creator>Babaei, Zahra</creator><creator>Sharbatkhori, Mitra</creator><creator>Shamsaddini, Saeedeh</creator><creator>Nasibi, Saeid</creator><creator>Harandi, Majid Fasihi</creator><general>Cambridge University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3257-5389</orcidid></search><sort><creationdate>201904</creationdate><title>Genetic and morphometric categorization of Taenia ovis from Sheep in Iran</title><author>Rostami, Sima ; Salavati, Reza ; Beech, Robin N. ; Babaei, Zahra ; Sharbatkhori, Mitra ; Shamsaddini, Saeedeh ; Nasibi, Saeid ; Harandi, Majid Fasihi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c373t-399272e1c6efaf85bc7363c30685fcd4e2295832de5cfa00f3ed43bbb435fa343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Abattoirs</topic><topic>Animals</topic><topic>Correlation coefficient</topic><topic>Correlation coefficients</topic><topic>Electron Transport Complex IV - analysis</topic><topic>Genes</topic><topic>Genetic diversity</topic><topic>Genetic Variation</topic><topic>Haplotypes</topic><topic>Helminth Proteins - analysis</topic><topic>Hooks</topic><topic>Iran</topic><topic>Larva - anatomy & histology</topic><topic>Mitochondria</topic><topic>Mitochondrial DNA</topic><topic>Mitochondrial Proteins - analysis</topic><topic>Morphometry</topic><topic>Mutation</topic><topic>Nucleotides</topic><topic>Parasites</topic><topic>Phylogeny</topic><topic>RNA, Helminth - analysis</topic><topic>RNA, Ribosomal - analysis</topic><topic>rRNA 12S</topic><topic>Sequence analysis</topic><topic>Sheep</topic><topic>Sheep Diseases - parasitology</topic><topic>Software</topic><topic>Taenia - anatomy & histology</topic><topic>Taenia - genetics</topic><topic>Taenia - growth & development</topic><topic>Taenia asiatica</topic><topic>Taenia ovis</topic><topic>Taenia ovis krabbei</topic><topic>Taenia ovis ovis</topic><topic>Taeniasis - parasitology</topic><topic>Taeniasis - veterinary</topic><topic>Vaccines</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rostami, Sima</creatorcontrib><creatorcontrib>Salavati, Reza</creatorcontrib><creatorcontrib>Beech, Robin N.</creatorcontrib><creatorcontrib>Babaei, Zahra</creatorcontrib><creatorcontrib>Sharbatkhori, Mitra</creatorcontrib><creatorcontrib>Shamsaddini, Saeedeh</creatorcontrib><creatorcontrib>Nasibi, Saeid</creatorcontrib><creatorcontrib>Harandi, Majid Fasihi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rostami, Sima</au><au>Salavati, Reza</au><au>Beech, Robin N.</au><au>Babaei, Zahra</au><au>Sharbatkhori, Mitra</au><au>Shamsaddini, Saeedeh</au><au>Nasibi, Saeid</au><au>Harandi, Majid Fasihi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic and morphometric categorization of Taenia ovis from Sheep in Iran</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2019-04</date><risdate>2019</risdate><volume>146</volume><issue>5</issue><spage>563</spage><epage>568</epage><pages>563-568</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><abstract>Little is known about the genetic and morphological characters of Taenia ovis. The purpose of the present study was to characterize sheep isolates of T. ovis using rostellar hook morphometry as well as mitochondrial genes sequence analysis. Ninety sheep specimens of Cysticercus ovis were collected from 18 slaughterhouses in Iran. The mean ± s.d. for total length of large and small hooks were 174.1 ± 6.4 and 116.7 ± 5.4 µm, respectively. CO1 and 12S rRNA sequence analysis showed 11 and nine haplotypes, respectively. The level of pairwise nucleotide variations between individual haplotypes of CO1 and 12S rRNA genes were 0.3–1.1 and 0.2–1.0%, respectively. Level of nucleotide variation in CO1 and 12S rRNA between T. ovis haplotypes from present study and eight other Taenia species was found to be 11.3–17.8 and 5.3–16.3%, respectively. Phylogenetic analysis clustered all T. ovis isolates into a single clade comprised of the all CO1 and 12S rRNA haplotypes. CO1 nucleotide difference between T. ovis ovis and T. asiatica was 13.6% that is lesser than the corresponding difference between T. ovis ovis and T. ovis krabbei, warranting the designation of two separate species as T. ovis and T. krabbei. Interclass correlation coefficients showed that there was no significant association between rostellar hook length variation and the variability of the mitochondrial genes.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>30419971</pmid><doi>10.1017/S0031182018001907</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-3257-5389</orcidid></addata></record> |
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| subjects | Abattoirs Animals Correlation coefficient Correlation coefficients Electron Transport Complex IV - analysis Genes Genetic diversity Genetic Variation Haplotypes Helminth Proteins - analysis Hooks Iran Larva - anatomy & histology Mitochondria Mitochondrial DNA Mitochondrial Proteins - analysis Morphometry Mutation Nucleotides Parasites Phylogeny RNA, Helminth - analysis RNA, Ribosomal - analysis rRNA 12S Sequence analysis Sheep Sheep Diseases - parasitology Software Taenia - anatomy & histology Taenia - genetics Taenia - growth & development Taenia asiatica Taenia ovis Taenia ovis krabbei Taenia ovis ovis Taeniasis - parasitology Taeniasis - veterinary Vaccines Worms |
| title | Genetic and morphometric categorization of Taenia ovis from Sheep in Iran |
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