A simple model for selection and rapid advancement of transgenic progeny in sorghum
To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct in planta assays for identifying the transgene carriers in the segregating progeny are ba...
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| Veröffentlicht in: | Plant biotechnology reports 2008-04, Vol.2 (1), p.47-58 |
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| creator | Visarada, K. B. R. S. Saikishore, N. Kuriakose, S. V. Shobha Rani, V. Royer, M. Rao, S. V. Seetharama, N. |
| description | To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct
in planta
assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of
bar
gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for
cry
1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing
bar
gene as selection marker using BASTA swab tests is described. |
| doi_str_mv | 10.1007/s11816-008-0048-5 |
| format | Article |
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in planta
assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of
bar
gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for
cry
1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing
bar
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in planta
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bar
gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for
cry
1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing
bar
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B. R. S.</au><au>Saikishore, N.</au><au>Kuriakose, S. V.</au><au>Shobha Rani, V.</au><au>Royer, M.</au><au>Rao, S. V.</au><au>Seetharama, N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A simple model for selection and rapid advancement of transgenic progeny in sorghum</atitle><jtitle>Plant biotechnology reports</jtitle><stitle>Plant Biotechnol Rep</stitle><date>2008-04-01</date><risdate>2008</risdate><volume>2</volume><issue>1</issue><spage>47</spage><epage>58</epage><pages>47-58</pages><issn>1863-5466</issn><eissn>1863-5474</eissn><abstract>To select agronomically useful transgenic plants, a large number of transgenic events are initially produced, gene transfer confirmed, and advanced to obtain homozygous lines for testing in field trials. Direct
in planta
assays for identifying the transgene carriers in the segregating progeny are based on the activity of selectable marker gene and are easy, simple and inexpensive. For this purpose, expression of
bar
gene as measured by tolerance to damage by glufosinate ammonium, the active ingredient in the herbicide BASTA, was investigated. Dose damage curves were generated by leaf paint tests with BASTA on four genotypes of sorghum. Transgenic plants were characterized in terms of sensitivity to the concentration of glufosinate ammonium. In transgenics, symptoms of BASTA swab tests at different growth stages and PCR analysis for
cry
1B were carried out and correlated. Germination tests could not be employed for large scale evaluation of transgenic progeny because of mortality of tolerant seedlings after transplantation to soil. Based on the above findings, a simple, inexpensive, time-saving, two-step scheme for effective evaluation of transgenics and their progeny containing
bar
gene as selection marker using BASTA swab tests is described.</abstract><cop>Japan</cop><pub>Springer Japan</pub><doi>10.1007/s11816-008-0048-5</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Plant biotechnology reports, 2008-04, Vol.2 (1), p.47-58 |
| issn | 1863-5466 1863-5474 |
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| source | SpringerLink Journals |
| subjects | Agriculture Ammonium Antibiotics Biomedical and Life Sciences Biotechnology Cell Biology Enzymes Genes Genotypes Herbicides Leaves Life Sciences Original Article Plant Biochemistry Plant biology Plant Sciences Seedlings Seeds Sorghum Studies Transgenic plants |
| title | A simple model for selection and rapid advancement of transgenic progeny in sorghum |
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