A Modified Method using TRIzol super(+) Reagent and Liquid Nitrogen Produces High-Quality RNA from Rat Pancreas

To establish an economical and reproducible method for the high-quality RNA extraction from pancreas, we isolated total RNA from rat pancreas with TRIzol+ reagent and liquid nitrogen. In the initial stage, we optimized three influential factors, the way to homogenize pancreas, the time to collect th...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2009-08, Vol.158 (2), p.253-261
Hauptverfasser:	Li, Dongmin, Ren, Wuchao, Wang, Xuan, Wang, Feimiao, Gao, Yu, Ning, Qilan, Han, Yan, Song, Tianbao, Lu, Shemin
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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