Measuring the functionality of the mitochondrial pumping complexes with multi-wavelength spectroscopy

The proton pumps of the mitochondrial electron transport chain (ETC) convert redox energy into the proton motive force (ΔP), which is subsequently used by the ATP synthase to regenerate ATP. The limited available redox free energy requires the proton pumps to operate close to equilibrium in order to...

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Veröffentlicht in:	Biochimica et biophysica acta. Bioenergetics 2019-01, Vol.1860 (1), p.89-101
Hauptverfasser:	Rocha, Mariana, Springett, Roger
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals ATP Electron transport chain Electron Transport Complex I - metabolism Electron Transport Complex III - metabolism Electron Transport Complex IV - metabolism Equilibrium Free energy Mice Mitochondria - chemistry Mitochondria - metabolism Oxidation-Reduction Oxygen Consumption Proton pumping Proton Pumps - physiology Proton-Motive Force - physiology RAW 264.7 Cells Spectrum Analysis - methods Turnover
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