Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies
Summary Background Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of Propionibacterium acnes (P. acnes) on pilosebaceous follicles. Aims To determine the efficacy of two different plant extracts against P. acnes and to analyze the g...
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| Veröffentlicht in: | Journal of cosmetic dermatology 2019-08, Vol.18 (4), p.1061-1065 |
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| container_title | Journal of cosmetic dermatology |
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| creator | Kılıç, Songül Okullu, Sinem Öktem Kurt, Özgür Sevinç, Hakan Dündar, Cihat Altınordu, Fahim Türkoğlu, Murat |
| description | Summary
Background
Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of Propionibacterium acnes (P. acnes) on pilosebaceous follicles.
Aims
To determine the efficacy of two different plant extracts against P. acnes and to analyze the gene expression levels of IL‐1α, SRD5A1, and TNFα in HaCaT cells treated with these plant extracts.
Methods
Anti‐acne extract 1 (AE1) consisted of Juglans regia (walnut husk), Myrtus communis (myrtle leaves), Matricaria chamomilla (chamomilla flowers), Urtica dioica (stinging nettle leaves), and Rosa damascena (rose flowers). Anti‐acne extract 2 (AE2) contained Brassica oleracea var. botrytis (broccoli) and B. oleracea var. italica (cauliflower). The antimicrobial activities of the extracts were tested on two different P. acnes strains: the reference strain of P. acnes (ATCC 51277) and the clinical isolate from a patient. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth dilution method. Human keratinocyte cells were used for in vitro tests. Gene expression analyses were performed with RT‐qPCR.
Results
The MIC values of the extracts were below 1/2048 µg/mL. In the gene expression analysis, AE1 increased the expression level of TNFα (1.1719, P |
| doi_str_mv | 10.1111/jocd.12814 |
| format | Article |
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Background
Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of Propionibacterium acnes (P. acnes) on pilosebaceous follicles.
Aims
To determine the efficacy of two different plant extracts against P. acnes and to analyze the gene expression levels of IL‐1α, SRD5A1, and TNFα in HaCaT cells treated with these plant extracts.
Methods
Anti‐acne extract 1 (AE1) consisted of Juglans regia (walnut husk), Myrtus communis (myrtle leaves), Matricaria chamomilla (chamomilla flowers), Urtica dioica (stinging nettle leaves), and Rosa damascena (rose flowers). Anti‐acne extract 2 (AE2) contained Brassica oleracea var. botrytis (broccoli) and B. oleracea var. italica (cauliflower). The antimicrobial activities of the extracts were tested on two different P. acnes strains: the reference strain of P. acnes (ATCC 51277) and the clinical isolate from a patient. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth dilution method. Human keratinocyte cells were used for in vitro tests. Gene expression analyses were performed with RT‐qPCR.
Results
The MIC values of the extracts were below 1/2048 µg/mL. In the gene expression analysis, AE1 increased the expression level of TNFα (1.1719, P < 0.0001), suppressed the expression level of IL‐1α, SRD5A1 (0.0588, P = 0.0231; 0.3081, P = 0.0351), respectively. AE2 suppressed gene expression level of IL‐1α, SRD5A1, TNFα (0.3815, P = 0.0254; 0.3418, P = 0.0271; 0.1997, P = 0.0623).
Conclusions
Both herbal extracts demonstrated strong antibacterial and anti‐inflammatory activity in this preliminary trial. In conclusion, the topical application of these botanical extracts can be good candidates for local acne treatment.</description><identifier>ISSN: 1473-2130</identifier><identifier>EISSN: 1473-2165</identifier><identifier>DOI: 10.1111/jocd.12814</identifier><identifier>PMID: 30414245</identifier><language>eng</language><publisher>England</publisher><subject>acne ; Acne Vulgaris - drug therapy ; Acne Vulgaris - microbiology ; Anti-Bacterial Agents - isolation & purification ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - therapeutic use ; Anti-Inflammatory Agents - isolation & purification ; Anti-Inflammatory Agents - pharmacology ; Anti-Inflammatory Agents - therapeutic use ; broth dilution method ; Cell Line ; Cell Proliferation - drug effects ; Flowers - chemistry ; HaCaT ; Humans ; IL‐1α ; Keratinocytes - drug effects ; Keratinocytes - metabolism ; Microbial Sensitivity Tests ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology ; Plant Extracts - therapeutic use ; Plant Leaves - chemistry ; Propionibacterium acnes ; Propionibacterium acnes - drug effects ; SRD5A1 ; TNFα ; Toxicity Tests</subject><ispartof>Journal of cosmetic dermatology, 2019-08, Vol.18 (4), p.1061-1065</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3294-327be1b8763f9cc0d302c5e84cac5355b40fc9dca0150bc18101991c0d7b4b33</citedby><cites>FETCH-LOGICAL-c3294-327be1b8763f9cc0d302c5e84cac5355b40fc9dca0150bc18101991c0d7b4b33</cites><orcidid>0000-0001-5299-712X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjocd.12814$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjocd.12814$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30414245$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kılıç, Songül</creatorcontrib><creatorcontrib>Okullu, Sinem Öktem</creatorcontrib><creatorcontrib>Kurt, Özgür</creatorcontrib><creatorcontrib>Sevinç, Hakan</creatorcontrib><creatorcontrib>Dündar, Cihat</creatorcontrib><creatorcontrib>Altınordu, Fahim</creatorcontrib><creatorcontrib>Türkoğlu, Murat</creatorcontrib><title>Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies</title><title>Journal of cosmetic dermatology</title><addtitle>J Cosmet Dermatol</addtitle><description>Summary
Background
Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of Propionibacterium acnes (P. acnes) on pilosebaceous follicles.
Aims
To determine the efficacy of two different plant extracts against P. acnes and to analyze the gene expression levels of IL‐1α, SRD5A1, and TNFα in HaCaT cells treated with these plant extracts.
Methods
Anti‐acne extract 1 (AE1) consisted of Juglans regia (walnut husk), Myrtus communis (myrtle leaves), Matricaria chamomilla (chamomilla flowers), Urtica dioica (stinging nettle leaves), and Rosa damascena (rose flowers). Anti‐acne extract 2 (AE2) contained Brassica oleracea var. botrytis (broccoli) and B. oleracea var. italica (cauliflower). The antimicrobial activities of the extracts were tested on two different P. acnes strains: the reference strain of P. acnes (ATCC 51277) and the clinical isolate from a patient. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth dilution method. Human keratinocyte cells were used for in vitro tests. Gene expression analyses were performed with RT‐qPCR.
Results
The MIC values of the extracts were below 1/2048 µg/mL. In the gene expression analysis, AE1 increased the expression level of TNFα (1.1719, P < 0.0001), suppressed the expression level of IL‐1α, SRD5A1 (0.0588, P = 0.0231; 0.3081, P = 0.0351), respectively. AE2 suppressed gene expression level of IL‐1α, SRD5A1, TNFα (0.3815, P = 0.0254; 0.3418, P = 0.0271; 0.1997, P = 0.0623).
Conclusions
Both herbal extracts demonstrated strong antibacterial and anti‐inflammatory activity in this preliminary trial. In conclusion, the topical application of these botanical extracts can be good candidates for local acne treatment.</description><subject>acne</subject><subject>Acne Vulgaris - drug therapy</subject><subject>Acne Vulgaris - microbiology</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Anti-Bacterial Agents - therapeutic use</subject><subject>Anti-Inflammatory Agents - isolation & purification</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Anti-Inflammatory Agents - therapeutic use</subject><subject>broth dilution method</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Flowers - chemistry</subject><subject>HaCaT</subject><subject>Humans</subject><subject>IL‐1α</subject><subject>Keratinocytes - drug effects</subject><subject>Keratinocytes - metabolism</subject><subject>Microbial Sensitivity Tests</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Extracts - therapeutic use</subject><subject>Plant Leaves - chemistry</subject><subject>Propionibacterium acnes</subject><subject>Propionibacterium acnes - drug effects</subject><subject>SRD5A1</subject><subject>TNFα</subject><subject>Toxicity Tests</subject><issn>1473-2130</issn><issn>1473-2165</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OwzAURi0EoqWw8ADII0JK8fVPk7ChUqCoUpfukeM4lSs3KXZC6dvjkNKRu_gO5376fBC6BTKGMI-bWhVjoAnwMzQEHrOIwkScn3ZGBujK-w0hEKcgLtGAEQ6ccjFE-1lZGiXVAdclbvY13llZNVh_N06qxmO5lqbyDZaq0virtWvpjH_C88o0RlrstG9twMLx1ihX56a29ToEWtxo391XBVbaWqwC1zqNfdMWRvtrdFFK6_XN8R2h1etsNX2PFsu3-fR5ESlGUx4xGuca8iSesDJVihSMUCV0wkNlwYTIOSlVWihJQJBcQQIE0hQCGOc8Z2yE7vvYnas_29Ao2xrf9ZGVrlufBTmUimQiaEAfejR8w3uny2znzFa6QwYk6zxnnefs13OA7465bb7VxQn9ExsA6IG9sfrwT1T2sZy-9KE_zDGJ1A</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Kılıç, Songül</creator><creator>Okullu, Sinem Öktem</creator><creator>Kurt, Özgür</creator><creator>Sevinç, Hakan</creator><creator>Dündar, Cihat</creator><creator>Altınordu, Fahim</creator><creator>Türkoğlu, Murat</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5299-712X</orcidid></search><sort><creationdate>201908</creationdate><title>Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies</title><author>Kılıç, Songül ; Okullu, Sinem Öktem ; Kurt, Özgür ; Sevinç, Hakan ; Dündar, Cihat ; Altınordu, Fahim ; Türkoğlu, Murat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3294-327be1b8763f9cc0d302c5e84cac5355b40fc9dca0150bc18101991c0d7b4b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>acne</topic><topic>Acne Vulgaris - drug therapy</topic><topic>Acne Vulgaris - microbiology</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Anti-Bacterial Agents - therapeutic use</topic><topic>Anti-Inflammatory Agents - isolation & purification</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Anti-Inflammatory Agents - therapeutic use</topic><topic>broth dilution method</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Flowers - chemistry</topic><topic>HaCaT</topic><topic>Humans</topic><topic>IL‐1α</topic><topic>Keratinocytes - drug effects</topic><topic>Keratinocytes - metabolism</topic><topic>Microbial Sensitivity Tests</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Extracts - therapeutic use</topic><topic>Plant Leaves - chemistry</topic><topic>Propionibacterium acnes</topic><topic>Propionibacterium acnes - drug effects</topic><topic>SRD5A1</topic><topic>TNFα</topic><topic>Toxicity Tests</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kılıç, Songül</creatorcontrib><creatorcontrib>Okullu, Sinem Öktem</creatorcontrib><creatorcontrib>Kurt, Özgür</creatorcontrib><creatorcontrib>Sevinç, Hakan</creatorcontrib><creatorcontrib>Dündar, Cihat</creatorcontrib><creatorcontrib>Altınordu, Fahim</creatorcontrib><creatorcontrib>Türkoğlu, Murat</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cosmetic dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kılıç, Songül</au><au>Okullu, Sinem Öktem</au><au>Kurt, Özgür</au><au>Sevinç, Hakan</au><au>Dündar, Cihat</au><au>Altınordu, Fahim</au><au>Türkoğlu, Murat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies</atitle><jtitle>Journal of cosmetic dermatology</jtitle><addtitle>J Cosmet Dermatol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>18</volume><issue>4</issue><spage>1061</spage><epage>1065</epage><pages>1061-1065</pages><issn>1473-2130</issn><eissn>1473-2165</eissn><abstract>Summary
Background
Acne vulgaris is a common skin disease characterized by increased sebum production, inflammation, and colonization of Propionibacterium acnes (P. acnes) on pilosebaceous follicles.
Aims
To determine the efficacy of two different plant extracts against P. acnes and to analyze the gene expression levels of IL‐1α, SRD5A1, and TNFα in HaCaT cells treated with these plant extracts.
Methods
Anti‐acne extract 1 (AE1) consisted of Juglans regia (walnut husk), Myrtus communis (myrtle leaves), Matricaria chamomilla (chamomilla flowers), Urtica dioica (stinging nettle leaves), and Rosa damascena (rose flowers). Anti‐acne extract 2 (AE2) contained Brassica oleracea var. botrytis (broccoli) and B. oleracea var. italica (cauliflower). The antimicrobial activities of the extracts were tested on two different P. acnes strains: the reference strain of P. acnes (ATCC 51277) and the clinical isolate from a patient. The minimum inhibitory concentration (MIC) of the extracts was determined using the broth dilution method. Human keratinocyte cells were used for in vitro tests. Gene expression analyses were performed with RT‐qPCR.
Results
The MIC values of the extracts were below 1/2048 µg/mL. In the gene expression analysis, AE1 increased the expression level of TNFα (1.1719, P < 0.0001), suppressed the expression level of IL‐1α, SRD5A1 (0.0588, P = 0.0231; 0.3081, P = 0.0351), respectively. AE2 suppressed gene expression level of IL‐1α, SRD5A1, TNFα (0.3815, P = 0.0254; 0.3418, P = 0.0271; 0.1997, P = 0.0623).
Conclusions
Both herbal extracts demonstrated strong antibacterial and anti‐inflammatory activity in this preliminary trial. In conclusion, the topical application of these botanical extracts can be good candidates for local acne treatment.</abstract><cop>England</cop><pmid>30414245</pmid><doi>10.1111/jocd.12814</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-5299-712X</orcidid></addata></record> |
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| subjects | acne Acne Vulgaris - drug therapy Acne Vulgaris - microbiology Anti-Bacterial Agents - isolation & purification Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - therapeutic use Anti-Inflammatory Agents - isolation & purification Anti-Inflammatory Agents - pharmacology Anti-Inflammatory Agents - therapeutic use broth dilution method Cell Line Cell Proliferation - drug effects Flowers - chemistry HaCaT Humans IL‐1α Keratinocytes - drug effects Keratinocytes - metabolism Microbial Sensitivity Tests Plant Extracts - isolation & purification Plant Extracts - pharmacology Plant Extracts - therapeutic use Plant Leaves - chemistry Propionibacterium acnes Propionibacterium acnes - drug effects SRD5A1 TNFα Toxicity Tests |
| title | Efficacy of two plant extracts against acne vulgaris: Initial results of microbiological tests and cell culture studies |
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