Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles
In this presented work, magnetic poly(2-hydroxyethyl methacrylate) (p (HEMA)) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Cibacron Blue F3GA was covalently attached to the magnetic p (HEMA) nanoparticles and Cu 2+ ions were then chelated with dye molecules. S...
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| Veröffentlicht in: | Applied biochemistry and biotechnology 2019-05, Vol.188 (1), p.194-207 |
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| creator | Evli, Sinem Uygun, Deniz Aktaş |
| description | In this presented work, magnetic poly(2-hydroxyethyl methacrylate) (p (HEMA)) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Cibacron Blue F3GA was covalently attached to the magnetic p (HEMA) nanoparticles and Cu
2+
ions were then chelated with dye molecules. Synthesized magnetic nanoparticles were spherical with the diameter of 80 nm and exhibited magnetic character. Incorporation rate of Cibacron Blue for magnetic nanoparticles was found to be 28.125-μmol/g polymer. Loaded amount of Cu
2+
ions was calculated as 10.229-μmol/g polymer. These Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles were used for urokinase adsorption under different conditions (i.e., pH, enzyme initial concentration, ionic strength, temperature). Maximum adsorption capacity was found to be 630.43-mg/g polymer, and it was observed that Langmuir adsorption isotherm was applicable in this adsorption process. The adsorbed urokinase was desorbed from the Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles by using 1.0 M of NaCl with the desorption rate of 96%. It was also demonstrated that adsorption capacity did not change significantly after five adsorption/desorption cycles. |
| doi_str_mv | 10.1007/s12010-018-2923-z |
| format | Article |
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2+
ions were then chelated with dye molecules. Synthesized magnetic nanoparticles were spherical with the diameter of 80 nm and exhibited magnetic character. Incorporation rate of Cibacron Blue for magnetic nanoparticles was found to be 28.125-μmol/g polymer. Loaded amount of Cu
2+
ions was calculated as 10.229-μmol/g polymer. These Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles were used for urokinase adsorption under different conditions (i.e., pH, enzyme initial concentration, ionic strength, temperature). Maximum adsorption capacity was found to be 630.43-mg/g polymer, and it was observed that Langmuir adsorption isotherm was applicable in this adsorption process. The adsorbed urokinase was desorbed from the Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles by using 1.0 M of NaCl with the desorption rate of 96%. It was also demonstrated that adsorption capacity did not change significantly after five adsorption/desorption cycles.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-018-2923-z</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adsorption ; Biochemistry ; Biotechnology ; Chemical synthesis ; Chemistry ; Chemistry and Materials Science ; Cibacron Blue F3GA ; Copper ; Desorption ; Emulsion polymerization ; Enzymatic activity ; Ionic strength ; Ions ; Nanoparticles ; Polyhydroxyethyl methacrylate ; Polymerization ; Polymers ; Sodium chloride ; U-Plasminogen activator ; Urokinase</subject><ispartof>Applied biochemistry and biotechnology, 2019-05, Vol.188 (1), p.194-207</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Applied Biochemistry and Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-bd8763394ffe9688653c052472f3239f4050eb634fb28eb675cef0b0103c69f93</citedby><cites>FETCH-LOGICAL-c316t-bd8763394ffe9688653c052472f3239f4050eb634fb28eb675cef0b0103c69f93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12010-018-2923-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12010-018-2923-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Evli, Sinem</creatorcontrib><creatorcontrib>Uygun, Deniz Aktaş</creatorcontrib><title>Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><description>In this presented work, magnetic poly(2-hydroxyethyl methacrylate) (p (HEMA)) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Cibacron Blue F3GA was covalently attached to the magnetic p (HEMA) nanoparticles and Cu
2+
ions were then chelated with dye molecules. Synthesized magnetic nanoparticles were spherical with the diameter of 80 nm and exhibited magnetic character. Incorporation rate of Cibacron Blue for magnetic nanoparticles was found to be 28.125-μmol/g polymer. Loaded amount of Cu
2+
ions was calculated as 10.229-μmol/g polymer. These Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles were used for urokinase adsorption under different conditions (i.e., pH, enzyme initial concentration, ionic strength, temperature). Maximum adsorption capacity was found to be 630.43-mg/g polymer, and it was observed that Langmuir adsorption isotherm was applicable in this adsorption process. The adsorbed urokinase was desorbed from the Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles by using 1.0 M of NaCl with the desorption rate of 96%. It was also demonstrated that adsorption capacity did not change significantly after five adsorption/desorption cycles.</description><subject>Adsorption</subject><subject>Biochemistry</subject><subject>Biotechnology</subject><subject>Chemical synthesis</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Cibacron Blue F3GA</subject><subject>Copper</subject><subject>Desorption</subject><subject>Emulsion polymerization</subject><subject>Enzymatic activity</subject><subject>Ionic strength</subject><subject>Ions</subject><subject>Nanoparticles</subject><subject>Polyhydroxyethyl methacrylate</subject><subject>Polymerization</subject><subject>Polymers</subject><subject>Sodium chloride</subject><subject>U-Plasminogen activator</subject><subject>Urokinase</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kd9uFCEUxonRxLX1Abwj8abG0MJhGIbLdbr9k7Tqhb0mzBQqdQZWmGmye2F8B9_QJ5HNNjFp0iu-wO87h3M-hN4xeswolSeZAWWUUNYQUMDJ9gVaMCEUoaDYS7SgIDkBaNRr9Cbne0oZNEIu0K9V2G5GM_keL_vJP_hpg6PDNyn-8MFkiy_HMXZ-8Ft7i2OYIm5n-Eja73YwU7lqfWf6FAP-NMwWn_Hz5d_ff05t8g_l8WscNvjoYnW9_ICvzV2wuzafTYhrk4ocbD5Er5wZsn37eB6gm7PVt_aCXH05v2yXV6TnrJ5Id9vImnNVOWdV3TS14D0VUElwHLhyFRXUdjWvXAdNEVL01tGubIT3tXKKH6Cjfd11ij9nmyc9-tzbYTDBxjlrYBxACAl1Qd8_Qe_jnEL53Y5ismK1hEKxPVWGzzlZp9fJjyZtNKN6l4jeJ6JLInqXiN4WD-w9ubDhzqb_lZ83_QM2-Y3l</recordid><startdate>20190501</startdate><enddate>20190501</enddate><creator>Evli, Sinem</creator><creator>Uygun, Deniz Aktaş</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20190501</creationdate><title>Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles</title><author>Evli, Sinem ; Uygun, Deniz Aktaş</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-bd8763394ffe9688653c052472f3239f4050eb634fb28eb675cef0b0103c69f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adsorption</topic><topic>Biochemistry</topic><topic>Biotechnology</topic><topic>Chemical synthesis</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Cibacron Blue F3GA</topic><topic>Copper</topic><topic>Desorption</topic><topic>Emulsion polymerization</topic><topic>Enzymatic activity</topic><topic>Ionic strength</topic><topic>Ions</topic><topic>Nanoparticles</topic><topic>Polyhydroxyethyl methacrylate</topic><topic>Polymerization</topic><topic>Polymers</topic><topic>Sodium chloride</topic><topic>U-Plasminogen activator</topic><topic>Urokinase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Evli, Sinem</creatorcontrib><creatorcontrib>Uygun, Deniz Aktaş</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Evli, Sinem</au><au>Uygun, Deniz Aktaş</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><date>2019-05-01</date><risdate>2019</risdate><volume>188</volume><issue>1</issue><spage>194</spage><epage>207</epage><pages>194-207</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>In this presented work, magnetic poly(2-hydroxyethyl methacrylate) (p (HEMA)) nanoparticles were synthesized by surfactant-free emulsion polymerization technique. Cibacron Blue F3GA was covalently attached to the magnetic p (HEMA) nanoparticles and Cu
2+
ions were then chelated with dye molecules. Synthesized magnetic nanoparticles were spherical with the diameter of 80 nm and exhibited magnetic character. Incorporation rate of Cibacron Blue for magnetic nanoparticles was found to be 28.125-μmol/g polymer. Loaded amount of Cu
2+
ions was calculated as 10.229-μmol/g polymer. These Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles were used for urokinase adsorption under different conditions (i.e., pH, enzyme initial concentration, ionic strength, temperature). Maximum adsorption capacity was found to be 630.43-mg/g polymer, and it was observed that Langmuir adsorption isotherm was applicable in this adsorption process. The adsorbed urokinase was desorbed from the Cu
2+
-Cibacron Blue F3GA–derived magnetic p (HEMA) nanoparticles by using 1.0 M of NaCl with the desorption rate of 96%. It was also demonstrated that adsorption capacity did not change significantly after five adsorption/desorption cycles.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s12010-018-2923-z</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0273-2289 |
| ispartof | Applied biochemistry and biotechnology, 2019-05, Vol.188 (1), p.194-207 |
| issn | 0273-2289 1559-0291 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2132255726 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Adsorption Biochemistry Biotechnology Chemical synthesis Chemistry Chemistry and Materials Science Cibacron Blue F3GA Copper Desorption Emulsion polymerization Enzymatic activity Ionic strength Ions Nanoparticles Polyhydroxyethyl methacrylate Polymerization Polymers Sodium chloride U-Plasminogen activator Urokinase |
| title | Enzymatic Activity of Urokinase Immobilized onto Cu2+-Chelated Cibacron Blue F3GA–Derived Poly (HEMA) Magnetic Nanoparticles |
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