Escherichia coli transketolase-catalyzed carbon-carbon bond formation: biotransformation characterization for reactor evaluation and selection
The conversion of glycolaldehyde and β-hydroxypyruvate to l-erythrulose and carbon dioxide was used as a representative condensation to determine the key data required for rapid process evaluation and the scaleup of useful transketolase-catalyzed biotransformations. The substrates and product exhibi...
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| Veröffentlicht in: | Enzyme and microbial technology 1998, Vol.22 (1), p.64-70 |
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| container_title | Enzyme and microbial technology |
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| creator | Mitra, Robin K. Woodley, John M. Lilly, Malcolm D. |
| description | The conversion of glycolaldehyde and β-hydroxypyruvate to
l-erythrulose and carbon dioxide was used as a representative condensation to determine the key data required for rapid process evaluation and the scaleup of useful transketolase-catalyzed biotransformations. The substrates and product exhibited alkaline lability requiring control of reaction pH. The holotransketolase was rapidly deactivated via oxidation and was unstable at pH values less than 6.5 and greater than 10.0. The strength of binding of the cofactors to the enzyme was low at all operational pH values with implications for enzyme immobilization. Glycolaldehyde was found to be toxic to the enzyme and consequently the role of substrate feeding strategies was examined. |
| doi_str_mv | 10.1016/S0141-0229(97)00106-3 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 1998, Vol.22 (1), p.64-70 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21314813 |
| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Aldehydes Biological and medical sciences Bioreactors Biotechnology biotransformation Coliform bacteria Enzyme immobilization Enzymes Escherichia coli Esters Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology reactor design Sugars Transketolase |
| title | Escherichia coli transketolase-catalyzed carbon-carbon bond formation: biotransformation characterization for reactor evaluation and selection |
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