Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior
The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems...
Gespeichert in:
Veröffentlicht in: | Enzyme and microbial technology 1998-02, Vol.22 (3), p.147-151 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 151 |
---|---|
container_issue | 3 |
container_start_page | 147 |
container_title | Enzyme and microbial technology |
container_volume | 22 |
creator | Bracey, Emma Stenning, Robert A. Brooker, Brian E. |
description | The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems. Sonication was shown to be effective in reducing particle size, but the effect of any reduction in diffusional limitations on reaction rate was exceeded by an inhibitory effect of the treatment. This inhibition was found to be irreversible upon cessation of sonication and aqueous assay of activity, or recycling of the enzyme into a stirred reaction system. |
doi_str_mv | 10.1016/S0141-0229(97)00138-5 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21309425</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0141022997001385</els_id><sourcerecordid>320975</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-37ce0989ad4bd8c00eebc16904cf8ac24881209601d5f1d2e4b6c819ebe606e53</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhoMoWKs_QchBRA-rk910d3MSKX5BQfDjHLLZ2Ta6m9QkLdRfb7ctvXqawzzvvMxDyDmDGwYsv30HxlkCaSquRHENwLIyGR2QASsLkYAAcUgGe-SYnITwBWuKcxgQ-YatisZOaZwh7Yz2LkS_0HHhkbqGov1ddUhrE-bog3E2UGOp81NljabBtUu0MdDo-rzx9NtYjOtNhTO1NM6fkqNGtQHPdnNIPh8fPsbPyeT16WV8P0k0Bx6TrNAIohSq5lVdagDESrNcANdNqXTKy5KlIHJg9ahhdYq8ynXJBFaYQ46jbEgut3fn3v0sMETZmaCxbZVFtwgyZRkInvbgaAv2nwaPjZx70ym_kgxkr1NudMrelRSF3OiUfe5iV6CCVm3jldUm7MMp41kOPXa3xXD97NKgl0EbtBpr41FHWTvzT9Efr0mLAQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21309425</pqid></control><display><type>article</type><title>Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior</title><source>Elsevier ScienceDirect Journals</source><creator>Bracey, Emma ; Stenning, Robert A. ; Brooker, Brian E.</creator><creatorcontrib>Bracey, Emma ; Stenning, Robert A. ; Brooker, Brian E.</creatorcontrib><description>The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems. Sonication was shown to be effective in reducing particle size, but the effect of any reduction in diffusional limitations on reaction rate was exceeded by an inhibitory effect of the treatment. This inhibition was found to be irreversible upon cessation of sonication and aqueous assay of activity, or recycling of the enzyme into a stirred reaction system.</description><identifier>ISSN: 0141-0229</identifier><identifier>EISSN: 1879-0909</identifier><identifier>DOI: 10.1016/S0141-0229(97)00138-5</identifier><identifier>CODEN: EMTED2</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Electron microscopy ; Enzyme inhibition ; Esterification ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Microstructure ; organic solvent ; Organic solvents ; particle size ; Particle size analysis ; Reaction kinetics ; Subtilisin ; Ultrasonic effects ; ultrasound irradiation</subject><ispartof>Enzyme and microbial technology, 1998-02, Vol.22 (3), p.147-151</ispartof><rights>1998</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-37ce0989ad4bd8c00eebc16904cf8ac24881209601d5f1d2e4b6c819ebe606e53</citedby><cites>FETCH-LOGICAL-c404t-37ce0989ad4bd8c00eebc16904cf8ac24881209601d5f1d2e4b6c819ebe606e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141022997001385$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27902,27903,65308</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2143605$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Bracey, Emma</creatorcontrib><creatorcontrib>Stenning, Robert A.</creatorcontrib><creatorcontrib>Brooker, Brian E.</creatorcontrib><title>Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior</title><title>Enzyme and microbial technology</title><description>The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems. Sonication was shown to be effective in reducing particle size, but the effect of any reduction in diffusional limitations on reaction rate was exceeded by an inhibitory effect of the treatment. This inhibition was found to be irreversible upon cessation of sonication and aqueous assay of activity, or recycling of the enzyme into a stirred reaction system.</description><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Electron microscopy</subject><subject>Enzyme inhibition</subject><subject>Esterification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microstructure</subject><subject>organic solvent</subject><subject>Organic solvents</subject><subject>particle size</subject><subject>Particle size analysis</subject><subject>Reaction kinetics</subject><subject>Subtilisin</subject><subject>Ultrasonic effects</subject><subject>ultrasound irradiation</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqFkE1LAzEQhoMoWKs_QchBRA-rk910d3MSKX5BQfDjHLLZ2Ta6m9QkLdRfb7ctvXqawzzvvMxDyDmDGwYsv30HxlkCaSquRHENwLIyGR2QASsLkYAAcUgGe-SYnITwBWuKcxgQ-YatisZOaZwh7Yz2LkS_0HHhkbqGov1ddUhrE-bog3E2UGOp81NljabBtUu0MdDo-rzx9NtYjOtNhTO1NM6fkqNGtQHPdnNIPh8fPsbPyeT16WV8P0k0Bx6TrNAIohSq5lVdagDESrNcANdNqXTKy5KlIHJg9ahhdYq8ynXJBFaYQ46jbEgut3fn3v0sMETZmaCxbZVFtwgyZRkInvbgaAv2nwaPjZx70ym_kgxkr1NudMrelRSF3OiUfe5iV6CCVm3jldUm7MMp41kOPXa3xXD97NKgl0EbtBpr41FHWTvzT9Efr0mLAQ</recordid><startdate>19980215</startdate><enddate>19980215</enddate><creator>Bracey, Emma</creator><creator>Stenning, Robert A.</creator><creator>Brooker, Brian E.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19980215</creationdate><title>Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior</title><author>Bracey, Emma ; Stenning, Robert A. ; Brooker, Brian E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-37ce0989ad4bd8c00eebc16904cf8ac24881209601d5f1d2e4b6c819ebe606e53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Electron microscopy</topic><topic>Enzyme inhibition</topic><topic>Esterification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microstructure</topic><topic>organic solvent</topic><topic>Organic solvents</topic><topic>particle size</topic><topic>Particle size analysis</topic><topic>Reaction kinetics</topic><topic>Subtilisin</topic><topic>Ultrasonic effects</topic><topic>ultrasound irradiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bracey, Emma</creatorcontrib><creatorcontrib>Stenning, Robert A.</creatorcontrib><creatorcontrib>Brooker, Brian E.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bracey, Emma</au><au>Stenning, Robert A.</au><au>Brooker, Brian E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior</atitle><jtitle>Enzyme and microbial technology</jtitle><date>1998-02-15</date><risdate>1998</risdate><volume>22</volume><issue>3</issue><spage>147</spage><epage>151</epage><pages>147-151</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>The effect of ultrasound irradiation on activity and particle size of a subtilisin-catalyzed interesterification reaction in an organic solvent was studied. Across a range of levels of water addition and irradiation amplitude, sonication failed to increase reaction rate compared with stirred systems. Sonication was shown to be effective in reducing particle size, but the effect of any reduction in diffusional limitations on reaction rate was exceeded by an inhibitory effect of the treatment. This inhibition was found to be irreversible upon cessation of sonication and aqueous assay of activity, or recycling of the enzyme into a stirred reaction system.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><doi>10.1016/S0141-0229(97)00138-5</doi><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0141-0229 |
ispartof | Enzyme and microbial technology, 1998-02, Vol.22 (3), p.147-151 |
issn | 0141-0229 1879-0909 |
language | eng |
recordid | cdi_proquest_miscellaneous_21309425 |
source | Elsevier ScienceDirect Journals |
subjects | Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Electron microscopy Enzyme inhibition Esterification Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microstructure organic solvent Organic solvents particle size Particle size analysis Reaction kinetics Subtilisin Ultrasonic effects ultrasound irradiation |
title | Relating the microstructure of enzyme dispersions in organic solvents to their kinetic behavior |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T10%3A05%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Relating%20the%20microstructure%20of%20enzyme%20dispersions%20in%20organic%20solvents%20to%20their%20kinetic%20behavior&rft.jtitle=Enzyme%20and%20microbial%20technology&rft.au=Bracey,%20Emma&rft.date=1998-02-15&rft.volume=22&rft.issue=3&rft.spage=147&rft.epage=151&rft.pages=147-151&rft.issn=0141-0229&rft.eissn=1879-0909&rft.coden=EMTED2&rft_id=info:doi/10.1016/S0141-0229(97)00138-5&rft_dat=%3Cproquest_cross%3E320975%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21309425&rft_id=info:pmid/&rft_els_id=S0141022997001385&rfr_iscdi=true |