Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex
Nutrients in the lumen of the small intestine are sensed by special cells in the epithelial lining. The ensuing neurohumoral reflexes affect gastrointestinal absorption/secretion, motility, and vascular perfusion. To study in vivo the effect of a monosaccharide (glucose) or polysaccharide (starch) p...
Gespeichert in:
| Veröffentlicht in: | Pflügers Archiv 2019-04, Vol.471 (4), p.595-603 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 603 |
|---|---|
| container_issue | 4 |
| container_start_page | 595 |
| container_title | Pflügers Archiv |
| container_volume | 471 |
| creator | Mourad, Fadi H. Barada, Kassem A. Saade, Nayef E. |
| description | Nutrients in the lumen of the small intestine are sensed by special cells in the epithelial lining. The ensuing neurohumoral reflexes affect gastrointestinal absorption/secretion, motility, and vascular perfusion. To study in vivo the effect of a monosaccharide (glucose) or polysaccharide (starch) present in the jejunum on glucose absorption from an adjacent part of the intestine and investigate the possible underlying mechanisms. Using the single pass intraluminal perfusion technique, a segment of jejunum (perfusion segment) was continuously perfused with 20 mM glucose to determine glucose absorption. One hour later, a bolus of a saccharide was instilled in an isolated adjacent jejunal segment and the change in glucose absorption was monitored for a further 2 h. The contribution of neural mechanisms in this process was investigated. Instillation of glucose (20 mM or 40 mM) in either distal or proximal jejunal pouch elicited immediate and sustained inhibition of glucose absorption (a decrease by 25%;
P
|
| doi_str_mv | 10.1007/s00424-018-2230-0 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2130803040</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2130803040</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-c80ceb4b42bcf11bdf0fbea650c75fd1d6ac1a107050c8ced4b21f7d2ba2cdab3</originalsourceid><addsrcrecordid>eNp1kc1u1TAQhS0EopfCA7BBltiwCcw4uUnEDlX8VKrEBtaWf8ato8S-2I7gvgWPjG9TilSJ1Ugz3zkz9mHsJcJbBBjeZYBOdA3g2AjRQgOP2A67VjQCsH3MdgAtNv3Qj2fsWc4TAIhuFE_ZWQsdiKHf79jvy3DjtS8xHTk5R6bw6Pi8Lj6omWdlzI1K3lLmMfDreTUxE1c6x3QovrZcigtXgSs7KUOh8Imm9VbqC3EfeFIlv-eKB_o5H3k1MslrsnVUKJfbLYHWVEsiN9Ov5-yJU3OmF3f1nH3_9PHbxZfm6uvny4sPV41pB1EaM4Ih3elOaOMQtXXgNKl-D2bYO4u2VwYVwgC1MxqynRboBiu0EsYq3Z6zN5vvIcUfaz1FLj4bmmcVKK5ZCmxhhNNHVfT1A3SKa6qXbxSOuO-HSuFGmRRzro-Rh-QXlY4SQZ7ikltcsgrkKS55cn5157zqhey94m8-FRAbkOsoXFP6t_r_rn8At62jPg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2130181567</pqid></control><display><type>article</type><title>Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex</title><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Mourad, Fadi H. ; Barada, Kassem A. ; Saade, Nayef E.</creator><creatorcontrib>Mourad, Fadi H. ; Barada, Kassem A. ; Saade, Nayef E.</creatorcontrib><description>Nutrients in the lumen of the small intestine are sensed by special cells in the epithelial lining. The ensuing neurohumoral reflexes affect gastrointestinal absorption/secretion, motility, and vascular perfusion. To study in vivo the effect of a monosaccharide (glucose) or polysaccharide (starch) present in the jejunum on glucose absorption from an adjacent part of the intestine and investigate the possible underlying mechanisms. Using the single pass intraluminal perfusion technique, a segment of jejunum (perfusion segment) was continuously perfused with 20 mM glucose to determine glucose absorption. One hour later, a bolus of a saccharide was instilled in an isolated adjacent jejunal segment and the change in glucose absorption was monitored for a further 2 h. The contribution of neural mechanisms in this process was investigated. Instillation of glucose (20 mM or 40 mM) in either distal or proximal jejunal pouch elicited immediate and sustained inhibition of glucose absorption (a decrease by 25%;
P
< 0.01) from the perfused jejunal segment. Comparable inhibition was obtained with instillation of other monosaccharides or starch in the jejunal pouch. This inhibition was abolished by adding tetrodotoxin to the pouch or to the perfused jejunal segment and also by pretreatment with sympathetic blockers (guanethidine or hexamethonium) and by chemical ablation of capsaicin-sensitive primary afferent fibers. Glucose absorption within the jejunum is auto-regulated through backward and forward mechanisms. This regulation is mediated by neural reflexes involving capsaicin-sensitive afferent and sympathetic efferent fibers. These reflexes might serve to protect against hyperglycemia.</description><identifier>ISSN: 0031-6768</identifier><identifier>EISSN: 1432-2013</identifier><identifier>DOI: 10.1007/s00424-018-2230-0</identifier><identifier>PMID: 30402765</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animals ; Biological Transport - drug effects ; Biomedical and Life Sciences ; Biomedicine ; Capsaicin ; Capsaicin - pharmacology ; Cell Biology ; Fibers ; Glucose ; Glucose - metabolism ; Human Physiology ; Hyperglycemia ; Intestinal Absorption - drug effects ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Jejunum ; Jejunum - drug effects ; Jejunum - metabolism ; Molecular Medicine ; Monosaccharides ; Neurosciences ; Nutrients ; Organ Physiology ; Perfusion ; Polysaccharides ; Polysaccharides - pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors ; Reflex - drug effects ; Reflexes ; Rodents ; Secretion ; Sensory neurons ; Small intestine ; Starch ; Tetrodotoxin ; Tetrodotoxin - pharmacology</subject><ispartof>Pflügers Archiv, 2019-04, Vol.471 (4), p.595-603</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Pflügers Archiv - European Journal of Physiology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-c80ceb4b42bcf11bdf0fbea650c75fd1d6ac1a107050c8ced4b21f7d2ba2cdab3</citedby><cites>FETCH-LOGICAL-c372t-c80ceb4b42bcf11bdf0fbea650c75fd1d6ac1a107050c8ced4b21f7d2ba2cdab3</cites><orcidid>0000-0002-1686-2807</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00424-018-2230-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00424-018-2230-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30402765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mourad, Fadi H.</creatorcontrib><creatorcontrib>Barada, Kassem A.</creatorcontrib><creatorcontrib>Saade, Nayef E.</creatorcontrib><title>Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex</title><title>Pflügers Archiv</title><addtitle>Pflugers Arch - Eur J Physiol</addtitle><addtitle>Pflugers Arch</addtitle><description>Nutrients in the lumen of the small intestine are sensed by special cells in the epithelial lining. The ensuing neurohumoral reflexes affect gastrointestinal absorption/secretion, motility, and vascular perfusion. To study in vivo the effect of a monosaccharide (glucose) or polysaccharide (starch) present in the jejunum on glucose absorption from an adjacent part of the intestine and investigate the possible underlying mechanisms. Using the single pass intraluminal perfusion technique, a segment of jejunum (perfusion segment) was continuously perfused with 20 mM glucose to determine glucose absorption. One hour later, a bolus of a saccharide was instilled in an isolated adjacent jejunal segment and the change in glucose absorption was monitored for a further 2 h. The contribution of neural mechanisms in this process was investigated. Instillation of glucose (20 mM or 40 mM) in either distal or proximal jejunal pouch elicited immediate and sustained inhibition of glucose absorption (a decrease by 25%;
P
< 0.01) from the perfused jejunal segment. Comparable inhibition was obtained with instillation of other monosaccharides or starch in the jejunal pouch. This inhibition was abolished by adding tetrodotoxin to the pouch or to the perfused jejunal segment and also by pretreatment with sympathetic blockers (guanethidine or hexamethonium) and by chemical ablation of capsaicin-sensitive primary afferent fibers. Glucose absorption within the jejunum is auto-regulated through backward and forward mechanisms. This regulation is mediated by neural reflexes involving capsaicin-sensitive afferent and sympathetic efferent fibers. These reflexes might serve to protect against hyperglycemia.</description><subject>Animals</subject><subject>Biological Transport - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Capsaicin</subject><subject>Capsaicin - pharmacology</subject><subject>Cell Biology</subject><subject>Fibers</subject><subject>Glucose</subject><subject>Glucose - metabolism</subject><subject>Human Physiology</subject><subject>Hyperglycemia</subject><subject>Intestinal Absorption - drug effects</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Jejunum</subject><subject>Jejunum - drug effects</subject><subject>Jejunum - metabolism</subject><subject>Molecular Medicine</subject><subject>Monosaccharides</subject><subject>Neurosciences</subject><subject>Nutrients</subject><subject>Organ Physiology</subject><subject>Perfusion</subject><subject>Polysaccharides</subject><subject>Polysaccharides - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors</subject><subject>Reflex - drug effects</subject><subject>Reflexes</subject><subject>Rodents</subject><subject>Secretion</subject><subject>Sensory neurons</subject><subject>Small intestine</subject><subject>Starch</subject><subject>Tetrodotoxin</subject><subject>Tetrodotoxin - pharmacology</subject><issn>0031-6768</issn><issn>1432-2013</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kc1u1TAQhS0EopfCA7BBltiwCcw4uUnEDlX8VKrEBtaWf8ato8S-2I7gvgWPjG9TilSJ1Ugz3zkz9mHsJcJbBBjeZYBOdA3g2AjRQgOP2A67VjQCsH3MdgAtNv3Qj2fsWc4TAIhuFE_ZWQsdiKHf79jvy3DjtS8xHTk5R6bw6Pi8Lj6omWdlzI1K3lLmMfDreTUxE1c6x3QovrZcigtXgSs7KUOh8Imm9VbqC3EfeFIlv-eKB_o5H3k1MslrsnVUKJfbLYHWVEsiN9Ov5-yJU3OmF3f1nH3_9PHbxZfm6uvny4sPV41pB1EaM4Ih3elOaOMQtXXgNKl-D2bYO4u2VwYVwgC1MxqynRboBiu0EsYq3Z6zN5vvIcUfaz1FLj4bmmcVKK5ZCmxhhNNHVfT1A3SKa6qXbxSOuO-HSuFGmRRzro-Rh-QXlY4SQZ7ikltcsgrkKS55cn5157zqhey94m8-FRAbkOsoXFP6t_r_rn8At62jPg</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Mourad, Fadi H.</creator><creator>Barada, Kassem A.</creator><creator>Saade, Nayef E.</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1686-2807</orcidid></search><sort><creationdate>20190401</creationdate><title>Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex</title><author>Mourad, Fadi H. ; Barada, Kassem A. ; Saade, Nayef E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-c80ceb4b42bcf11bdf0fbea650c75fd1d6ac1a107050c8ced4b21f7d2ba2cdab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biological Transport - drug effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Capsaicin</topic><topic>Capsaicin - pharmacology</topic><topic>Cell Biology</topic><topic>Fibers</topic><topic>Glucose</topic><topic>Glucose - metabolism</topic><topic>Human Physiology</topic><topic>Hyperglycemia</topic><topic>Intestinal Absorption - drug effects</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Jejunum</topic><topic>Jejunum - drug effects</topic><topic>Jejunum - metabolism</topic><topic>Molecular Medicine</topic><topic>Monosaccharides</topic><topic>Neurosciences</topic><topic>Nutrients</topic><topic>Organ Physiology</topic><topic>Perfusion</topic><topic>Polysaccharides</topic><topic>Polysaccharides - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors</topic><topic>Reflex - drug effects</topic><topic>Reflexes</topic><topic>Rodents</topic><topic>Secretion</topic><topic>Sensory neurons</topic><topic>Small intestine</topic><topic>Starch</topic><topic>Tetrodotoxin</topic><topic>Tetrodotoxin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mourad, Fadi H.</creatorcontrib><creatorcontrib>Barada, Kassem A.</creatorcontrib><creatorcontrib>Saade, Nayef E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Physical Education Index</collection><collection>Proquest Health and Medical Complete</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>Pflügers Archiv</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mourad, Fadi H.</au><au>Barada, Kassem A.</au><au>Saade, Nayef E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex</atitle><jtitle>Pflügers Archiv</jtitle><stitle>Pflugers Arch - Eur J Physiol</stitle><addtitle>Pflugers Arch</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>471</volume><issue>4</issue><spage>595</spage><epage>603</epage><pages>595-603</pages><issn>0031-6768</issn><eissn>1432-2013</eissn><abstract>Nutrients in the lumen of the small intestine are sensed by special cells in the epithelial lining. The ensuing neurohumoral reflexes affect gastrointestinal absorption/secretion, motility, and vascular perfusion. To study in vivo the effect of a monosaccharide (glucose) or polysaccharide (starch) present in the jejunum on glucose absorption from an adjacent part of the intestine and investigate the possible underlying mechanisms. Using the single pass intraluminal perfusion technique, a segment of jejunum (perfusion segment) was continuously perfused with 20 mM glucose to determine glucose absorption. One hour later, a bolus of a saccharide was instilled in an isolated adjacent jejunal segment and the change in glucose absorption was monitored for a further 2 h. The contribution of neural mechanisms in this process was investigated. Instillation of glucose (20 mM or 40 mM) in either distal or proximal jejunal pouch elicited immediate and sustained inhibition of glucose absorption (a decrease by 25%;
P
< 0.01) from the perfused jejunal segment. Comparable inhibition was obtained with instillation of other monosaccharides or starch in the jejunal pouch. This inhibition was abolished by adding tetrodotoxin to the pouch or to the perfused jejunal segment and also by pretreatment with sympathetic blockers (guanethidine or hexamethonium) and by chemical ablation of capsaicin-sensitive primary afferent fibers. Glucose absorption within the jejunum is auto-regulated through backward and forward mechanisms. This regulation is mediated by neural reflexes involving capsaicin-sensitive afferent and sympathetic efferent fibers. These reflexes might serve to protect against hyperglycemia.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30402765</pmid><doi>10.1007/s00424-018-2230-0</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-1686-2807</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-6768 |
| ispartof | Pflügers Archiv, 2019-04, Vol.471 (4), p.595-603 |
| issn | 0031-6768 1432-2013 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2130803040 |
| source | MEDLINE; SpringerLink (Online service) |
| subjects | Animals Biological Transport - drug effects Biomedical and Life Sciences Biomedicine Capsaicin Capsaicin - pharmacology Cell Biology Fibers Glucose Glucose - metabolism Human Physiology Hyperglycemia Intestinal Absorption - drug effects Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Jejunum Jejunum - drug effects Jejunum - metabolism Molecular Medicine Monosaccharides Neurosciences Nutrients Organ Physiology Perfusion Polysaccharides Polysaccharides - pharmacology Rats Rats, Sprague-Dawley Receptors Reflex - drug effects Reflexes Rodents Secretion Sensory neurons Small intestine Starch Tetrodotoxin Tetrodotoxin - pharmacology |
| title | Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A19%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inhibitory%20effect%20of%20luminal%20saccharides%20on%20glucose%20absorption%20from%20an%20adjacent%20jejunal%20site%20in%20rats:%20a%20newly%20described%20intestinal%20neural%20reflex&rft.jtitle=Pfl%C3%BCgers%20Archiv&rft.au=Mourad,%20Fadi%20H.&rft.date=2019-04-01&rft.volume=471&rft.issue=4&rft.spage=595&rft.epage=603&rft.pages=595-603&rft.issn=0031-6768&rft.eissn=1432-2013&rft_id=info:doi/10.1007/s00424-018-2230-0&rft_dat=%3Cproquest_cross%3E2130803040%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2130181567&rft_id=info:pmid/30402765&rfr_iscdi=true |