Spectrum-Resolved Triplex-Color Electrochemiluminescence Multiplexing Immunoassay with Highly-Passivated Nanocrystals as Tags
Nanocrystals (NCs) were extensively employed in optical-multiplexing with their size-dependent and narrow waveband photoluminescence (PL). Herein, to achieve NCs-based novel optical-multiplexing strategy with improved sensitivity, a spectrum-resolved triplex-color electrochemiluminescence (ECL) mult...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2018-11, Vol.90 (21), p.12361-12365 |
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| creator | Zhou, Jie Nie, Lei Zhang, Bin Zou, Guizheng |
| description | Nanocrystals (NCs) were extensively employed in optical-multiplexing with their size-dependent and narrow waveband photoluminescence (PL). Herein, to achieve NCs-based novel optical-multiplexing strategy with improved sensitivity, a spectrum-resolved triplex-color electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was proposed for the first time by extensively exploring the color-different monochromatic ECL from dual-stabilizers-capped CdTe and CdSe NCs and the inherent lower background of ECL than PL. As a proof of concept, carcinoembryonic antigen (CEA), prostate specific antigen (PSA), and alpha fetoprotein (AFP) were adopted as model analytes and formed three antigen-NCs pairs at the glassy carbon electrode surface via a one-pot immune-reaction with CdSe(550), CdTe(650), and CdTe(776) NCs as tags, respectively. The spectral ECL responses of three antigen-NCs pairs were simultaneously measured via one-pot ECL assay, and the maximum ECL intensity of each tag was directly utilized to determine corresponding antigen without any assistance from either optical filters or signal deconvolution. This ECL-MIA displayed negligible cross-reactivity, high color-selectivity, and excellent sensitivity with limits of detection down to 1, 10, and 0.01 pg/mL for CEA, PSA, and AFP, respectively, which might provide a promising alternative to NCs PL multiplexing. |
| doi_str_mv | 10.1021/acs.analchem.8b04424 |
| format | Article |
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Herein, to achieve NCs-based novel optical-multiplexing strategy with improved sensitivity, a spectrum-resolved triplex-color electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was proposed for the first time by extensively exploring the color-different monochromatic ECL from dual-stabilizers-capped CdTe and CdSe NCs and the inherent lower background of ECL than PL. As a proof of concept, carcinoembryonic antigen (CEA), prostate specific antigen (PSA), and alpha fetoprotein (AFP) were adopted as model analytes and formed three antigen-NCs pairs at the glassy carbon electrode surface via a one-pot immune-reaction with CdSe(550), CdTe(650), and CdTe(776) NCs as tags, respectively. The spectral ECL responses of three antigen-NCs pairs were simultaneously measured via one-pot ECL assay, and the maximum ECL intensity of each tag was directly utilized to determine corresponding antigen without any assistance from either optical filters or signal deconvolution. This ECL-MIA displayed negligible cross-reactivity, high color-selectivity, and excellent sensitivity with limits of detection down to 1, 10, and 0.01 pg/mL for CEA, PSA, and AFP, respectively, which might provide a promising alternative to NCs PL multiplexing.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.8b04424</identifier><identifier>PMID: 30350603</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Antigens ; Cadmium tellurides ; Carcinoembryonic antigen ; Color ; Color sensitivity ; Cross-reactivity ; Crystals ; Electrochemiluminescence ; Glassy carbon ; Immunoassay ; Intermetallic compounds ; Luminescence ; Multiplexing ; Nanocrystals ; Optical communication ; Optical filters ; Optics ; Photoluminescence ; Photons ; Prostate ; Selectivity ; Sensitivity ; Spectrum analysis ; Tags</subject><ispartof>Analytical chemistry (Washington), 2018-11, Vol.90 (21), p.12361-12365</ispartof><rights>Copyright American Chemical Society Nov 6, 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a422t-de2f8ed2704482fa483287736d1cfb4400004aaf0da1f0e2c44f1b16c459adcb3</citedby><cites>FETCH-LOGICAL-a422t-de2f8ed2704482fa483287736d1cfb4400004aaf0da1f0e2c44f1b16c459adcb3</cites><orcidid>0000-0002-1529-6356 ; 0000-0002-3295-3848</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.8b04424$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.8b04424$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30350603$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Nie, Lei</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><creatorcontrib>Zou, Guizheng</creatorcontrib><title>Spectrum-Resolved Triplex-Color Electrochemiluminescence Multiplexing Immunoassay with Highly-Passivated Nanocrystals as Tags</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Nanocrystals (NCs) were extensively employed in optical-multiplexing with their size-dependent and narrow waveband photoluminescence (PL). Herein, to achieve NCs-based novel optical-multiplexing strategy with improved sensitivity, a spectrum-resolved triplex-color electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was proposed for the first time by extensively exploring the color-different monochromatic ECL from dual-stabilizers-capped CdTe and CdSe NCs and the inherent lower background of ECL than PL. As a proof of concept, carcinoembryonic antigen (CEA), prostate specific antigen (PSA), and alpha fetoprotein (AFP) were adopted as model analytes and formed three antigen-NCs pairs at the glassy carbon electrode surface via a one-pot immune-reaction with CdSe(550), CdTe(650), and CdTe(776) NCs as tags, respectively. The spectral ECL responses of three antigen-NCs pairs were simultaneously measured via one-pot ECL assay, and the maximum ECL intensity of each tag was directly utilized to determine corresponding antigen without any assistance from either optical filters or signal deconvolution. This ECL-MIA displayed negligible cross-reactivity, high color-selectivity, and excellent sensitivity with limits of detection down to 1, 10, and 0.01 pg/mL for CEA, PSA, and AFP, respectively, which might provide a promising alternative to NCs PL multiplexing.</description><subject>Analytical chemistry</subject><subject>Antigens</subject><subject>Cadmium tellurides</subject><subject>Carcinoembryonic antigen</subject><subject>Color</subject><subject>Color sensitivity</subject><subject>Cross-reactivity</subject><subject>Crystals</subject><subject>Electrochemiluminescence</subject><subject>Glassy carbon</subject><subject>Immunoassay</subject><subject>Intermetallic compounds</subject><subject>Luminescence</subject><subject>Multiplexing</subject><subject>Nanocrystals</subject><subject>Optical communication</subject><subject>Optical filters</subject><subject>Optics</subject><subject>Photoluminescence</subject><subject>Photons</subject><subject>Prostate</subject><subject>Selectivity</subject><subject>Sensitivity</subject><subject>Spectrum analysis</subject><subject>Tags</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kUtPwzAQhC0EglL4BwhF4sIlZf1omh5RxUsqD0E5RxvHaY2cuNgJ0AP_HYcWDhw4WfZ-M6vxEHJEYUCB0TOUfoA1GrlQ1SDNQQgmtkiPDhnESZqybdIDAB6zEcAe2ff-BYBSoMku2ePAh5AA75HPp6WSjWur-FF5a95UEc2cXhr1EU-ssS66MN3cdlu0aStdKy9VLVV025rmG9T1PLqpqra26D2uonfdLKJrPV-YVfwQnvQbNsH2Dmsr3co3aHyEPprh3B-QnTJc1eHm7JPny4vZ5Dqe3l_dTM6nMQrGmrhQrExVEZIIkbISRcpZOhrxpKCyzIUIOUEgllAgLUExKURJc5pIMRxjIXPeJ6dr36Wzr63yTVbpEMMYrJVtfcYoh9F4zHkS0JM_6IttXfjnb4oxDsA6Sqwp6az3TpXZ0ukK3SqjkHX1ZKGe7KeebFNPkB1vzNu8UsWv6KePAMAa6OS_i__1_AImtKGf</recordid><startdate>20181106</startdate><enddate>20181106</enddate><creator>Zhou, Jie</creator><creator>Nie, Lei</creator><creator>Zhang, Bin</creator><creator>Zou, Guizheng</creator><general>American Chemical Society</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1529-6356</orcidid><orcidid>https://orcid.org/0000-0002-3295-3848</orcidid></search><sort><creationdate>20181106</creationdate><title>Spectrum-Resolved Triplex-Color Electrochemiluminescence Multiplexing Immunoassay with Highly-Passivated Nanocrystals as Tags</title><author>Zhou, Jie ; Nie, Lei ; Zhang, Bin ; Zou, Guizheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a422t-de2f8ed2704482fa483287736d1cfb4400004aaf0da1f0e2c44f1b16c459adcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analytical chemistry</topic><topic>Antigens</topic><topic>Cadmium tellurides</topic><topic>Carcinoembryonic antigen</topic><topic>Color</topic><topic>Color sensitivity</topic><topic>Cross-reactivity</topic><topic>Crystals</topic><topic>Electrochemiluminescence</topic><topic>Glassy carbon</topic><topic>Immunoassay</topic><topic>Intermetallic compounds</topic><topic>Luminescence</topic><topic>Multiplexing</topic><topic>Nanocrystals</topic><topic>Optical communication</topic><topic>Optical filters</topic><topic>Optics</topic><topic>Photoluminescence</topic><topic>Photons</topic><topic>Prostate</topic><topic>Selectivity</topic><topic>Sensitivity</topic><topic>Spectrum analysis</topic><topic>Tags</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Jie</creatorcontrib><creatorcontrib>Nie, Lei</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><creatorcontrib>Zou, Guizheng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Jie</au><au>Nie, Lei</au><au>Zhang, Bin</au><au>Zou, Guizheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spectrum-Resolved Triplex-Color Electrochemiluminescence Multiplexing Immunoassay with Highly-Passivated Nanocrystals as Tags</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2018-11-06</date><risdate>2018</risdate><volume>90</volume><issue>21</issue><spage>12361</spage><epage>12365</epage><pages>12361-12365</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>Nanocrystals (NCs) were extensively employed in optical-multiplexing with their size-dependent and narrow waveband photoluminescence (PL). Herein, to achieve NCs-based novel optical-multiplexing strategy with improved sensitivity, a spectrum-resolved triplex-color electrochemiluminescence (ECL) multiplexing immunoassay (MIA) was proposed for the first time by extensively exploring the color-different monochromatic ECL from dual-stabilizers-capped CdTe and CdSe NCs and the inherent lower background of ECL than PL. As a proof of concept, carcinoembryonic antigen (CEA), prostate specific antigen (PSA), and alpha fetoprotein (AFP) were adopted as model analytes and formed three antigen-NCs pairs at the glassy carbon electrode surface via a one-pot immune-reaction with CdSe(550), CdTe(650), and CdTe(776) NCs as tags, respectively. The spectral ECL responses of three antigen-NCs pairs were simultaneously measured via one-pot ECL assay, and the maximum ECL intensity of each tag was directly utilized to determine corresponding antigen without any assistance from either optical filters or signal deconvolution. This ECL-MIA displayed negligible cross-reactivity, high color-selectivity, and excellent sensitivity with limits of detection down to 1, 10, and 0.01 pg/mL for CEA, PSA, and AFP, respectively, which might provide a promising alternative to NCs PL multiplexing.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>30350603</pmid><doi>10.1021/acs.analchem.8b04424</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-1529-6356</orcidid><orcidid>https://orcid.org/0000-0002-3295-3848</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Analytical chemistry Antigens Cadmium tellurides Carcinoembryonic antigen Color Color sensitivity Cross-reactivity Crystals Electrochemiluminescence Glassy carbon Immunoassay Intermetallic compounds Luminescence Multiplexing Nanocrystals Optical communication Optical filters Optics Photoluminescence Photons Prostate Selectivity Sensitivity Spectrum analysis Tags |
| title | Spectrum-Resolved Triplex-Color Electrochemiluminescence Multiplexing Immunoassay with Highly-Passivated Nanocrystals as Tags |
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