Application of DNA-based techniques for intraspecies differentiation of hake fish
The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained o...
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| Veröffentlicht in: | Acta veterinaria Brno 2009-12, Vol.78 (4), p.673-678 |
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| creator | Hubalkova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) Kralik, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) Kasalova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) |
| description | The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained on the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection. |
| doi_str_mv | 10.2754/avb200978040673 |
| format | Article |
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Ustav Hygieny a Technologie Masa ; Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creator><creatorcontrib>Hubalkova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) ; Kralik, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) ; Kasalova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa ; Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creatorcontrib><description>The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained on the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection.</description><identifier>ISSN: 0001-7213</identifier><identifier>EISSN: 1801-7576</identifier><identifier>DOI: 10.2754/avb200978040673</identifier><language>eng</language><subject>ADN ; CITOCROMO B ; CONSUMER PROTECTION ; CYTOCHROME B ; DNA ; ETUDE DE MARCHE ; GENE ; GENES ; http://www.fao.org/aos/agrovoc#c_1826 ; http://www.fao.org/aos/agrovoc#c_2347 ; http://www.fao.org/aos/agrovoc#c_3214 ; http://www.fao.org/aos/agrovoc#c_32420 ; http://www.fao.org/aos/agrovoc#c_34079 ; http://www.fao.org/aos/agrovoc#c_34255 ; http://www.fao.org/aos/agrovoc#c_3791 ; http://www.fao.org/aos/agrovoc#c_41533 ; http://www.fao.org/aos/agrovoc#c_41534 ; http://www.fao.org/aos/agrovoc#c_41535 ; http://www.fao.org/aos/agrovoc#c_4618 ; http://www.fao.org/aos/agrovoc#c_6765 ; IDENTIFICACION ; IDENTIFICATION ; INVESTIGACION DE MERCADOS ; Marine ; MERLUCCIUS GAYI ; MERLUCCIUS HUBBSI ; MERLUCCIUS PRODUCTUS ; PCR ; PECES MARINOS ; POISSON DE MER ; PROTECCION DEL CONSUMIDOR ; PROTECTION DU CONSOMMATEUR ; RFLP ; SALTWATER FISHES</subject><ispartof>Acta veterinaria Brno, 2009-12, Vol.78 (4), p.673-678</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c335t-b1b8faa4adb2c28359235457fac32190bd9a3190c15fbbb8d9fb96701af19aae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Hubalkova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creatorcontrib><creatorcontrib>Kralik, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creatorcontrib><creatorcontrib>Kasalova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa</creatorcontrib><creatorcontrib>Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creatorcontrib><title>Application of DNA-based techniques for intraspecies differentiation of hake fish</title><title>Acta veterinaria Brno</title><description>The aim of this study was to determine hake species distribution on the Czech market by the PCR-RFLP and sequencing of mt cyt b, and to develop and optimise an alternative system for determination of hake species by sequencing and/or PCR-RFLP using Pan I sequence. Among 20 samples of hake obtained on the Czech market three species were identified: North Pacific hake (Merluccius productus), Argentine hake (Merluccius hubbsi) and South Pacific hake (Merluccius gayi). The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection.</description><subject>ADN</subject><subject>CITOCROMO B</subject><subject>CONSUMER PROTECTION</subject><subject>CYTOCHROME B</subject><subject>DNA</subject><subject>ETUDE DE MARCHE</subject><subject>GENE</subject><subject>GENES</subject><subject>http://www.fao.org/aos/agrovoc#c_1826</subject><subject>http://www.fao.org/aos/agrovoc#c_2347</subject><subject>http://www.fao.org/aos/agrovoc#c_3214</subject><subject>http://www.fao.org/aos/agrovoc#c_32420</subject><subject>http://www.fao.org/aos/agrovoc#c_34079</subject><subject>http://www.fao.org/aos/agrovoc#c_34255</subject><subject>http://www.fao.org/aos/agrovoc#c_3791</subject><subject>http://www.fao.org/aos/agrovoc#c_41533</subject><subject>http://www.fao.org/aos/agrovoc#c_41534</subject><subject>http://www.fao.org/aos/agrovoc#c_41535</subject><subject>http://www.fao.org/aos/agrovoc#c_4618</subject><subject>http://www.fao.org/aos/agrovoc#c_6765</subject><subject>IDENTIFICACION</subject><subject>IDENTIFICATION</subject><subject>INVESTIGACION DE MERCADOS</subject><subject>Marine</subject><subject>MERLUCCIUS GAYI</subject><subject>MERLUCCIUS HUBBSI</subject><subject>MERLUCCIUS PRODUCTUS</subject><subject>PCR</subject><subject>PECES MARINOS</subject><subject>POISSON DE MER</subject><subject>PROTECCION DEL CONSUMIDOR</subject><subject>PROTECTION DU CONSOMMATEUR</subject><subject>RFLP</subject><subject>SALTWATER FISHES</subject><issn>0001-7213</issn><issn>1801-7576</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNpdkDFPwzAQhS0EEqUwMyFlYgs920kcj1WhgFSBkGBhsc6OTQ1pHOwUiX9PqiIGpnc63fd07xFyTuGKibKY4ZdmAFLUUEAl-AGZ0BpoLkpRHZIJwG5mlB-Tk5TeAVghKz4hT_O-b73BwYcuCy67fpjnGpNtssGadec_tzZlLsTMd0PE1Fvjx0XjnbPRdoP_A9f4YTPn0_qUHDlskz371Sl5Wd48L-7y1ePt_WK-yg3n5ZBrqmuHWGCjmWE1LyXjZVEKh4YzKkE3EvmohpZOa1030mlZCaDoqES0fEou9759DLsvB7Xxydi2xc6GbVJjVhBidJ6S2f7QxJBStE710W8wfisKaled-lfdSFzsCYdB4Vv0SS1eGdCxRGCC8h9W_GtP</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Hubalkova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creator><creator>Kralik, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creator><creator>Kasalova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa</creator><creator>Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creator><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope></search><sort><creationdate>20091201</creationdate><title>Application of DNA-based techniques for intraspecies differentiation of hake fish</title><author>Hubalkova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) ; Kralik, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic) ; Kasalova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Masa ; Rencova, E.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c335t-b1b8faa4adb2c28359235457fac32190bd9a3190c15fbbb8d9fb96701af19aae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ADN</topic><topic>CITOCROMO B</topic><topic>CONSUMER PROTECTION</topic><topic>CYTOCHROME B</topic><topic>DNA</topic><topic>ETUDE DE MARCHE</topic><topic>GENE</topic><topic>GENES</topic><topic>http://www.fao.org/aos/agrovoc#c_1826</topic><topic>http://www.fao.org/aos/agrovoc#c_2347</topic><topic>http://www.fao.org/aos/agrovoc#c_3214</topic><topic>http://www.fao.org/aos/agrovoc#c_32420</topic><topic>http://www.fao.org/aos/agrovoc#c_34079</topic><topic>http://www.fao.org/aos/agrovoc#c_34255</topic><topic>http://www.fao.org/aos/agrovoc#c_3791</topic><topic>http://www.fao.org/aos/agrovoc#c_41533</topic><topic>http://www.fao.org/aos/agrovoc#c_41534</topic><topic>http://www.fao.org/aos/agrovoc#c_41535</topic><topic>http://www.fao.org/aos/agrovoc#c_4618</topic><topic>http://www.fao.org/aos/agrovoc#c_6765</topic><topic>IDENTIFICACION</topic><topic>IDENTIFICATION</topic><topic>INVESTIGACION DE MERCADOS</topic><topic>Marine</topic><topic>MERLUCCIUS GAYI</topic><topic>MERLUCCIUS HUBBSI</topic><topic>MERLUCCIUS PRODUCTUS</topic><topic>PCR</topic><topic>PECES MARINOS</topic><topic>POISSON DE MER</topic><topic>PROTECCION DEL CONSUMIDOR</topic><topic>PROTECTION DU CONSOMMATEUR</topic><topic>RFLP</topic><topic>SALTWATER FISHES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hubalkova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creatorcontrib><creatorcontrib>Kralik, P.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)</creatorcontrib><creatorcontrib>Kasalova, J.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). 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The approaches tested in our study represent a significant tool either for the differentiation of hake species from other gadoid species (PCR) or for intraspecies identification of different hake species (PCR-RFLP, sequencing). This knowledge can be applied in detection of fish species substitution within the consumers' rights protection.</abstract><doi>10.2754/avb200978040673</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Acta veterinaria Brno, 2009-12, Vol.78 (4), p.673-678 |
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| subjects | ADN CITOCROMO B CONSUMER PROTECTION CYTOCHROME B DNA ETUDE DE MARCHE GENE GENES http://www.fao.org/aos/agrovoc#c_1826 http://www.fao.org/aos/agrovoc#c_2347 http://www.fao.org/aos/agrovoc#c_3214 http://www.fao.org/aos/agrovoc#c_32420 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_34255 http://www.fao.org/aos/agrovoc#c_3791 http://www.fao.org/aos/agrovoc#c_41533 http://www.fao.org/aos/agrovoc#c_41534 http://www.fao.org/aos/agrovoc#c_41535 http://www.fao.org/aos/agrovoc#c_4618 http://www.fao.org/aos/agrovoc#c_6765 IDENTIFICACION IDENTIFICATION INVESTIGACION DE MERCADOS Marine MERLUCCIUS GAYI MERLUCCIUS HUBBSI MERLUCCIUS PRODUCTUS PCR PECES MARINOS POISSON DE MER PROTECCION DEL CONSUMIDOR PROTECTION DU CONSOMMATEUR RFLP SALTWATER FISHES |
| title | Application of DNA-based techniques for intraspecies differentiation of hake fish |
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