Antisense regulation of human gene MAP3K13: True phenomenon or artifact?
Antisense regulation of gene expression is a widespread but poorly understood mechanism of gene expression regulation. The potential role of antisense transcripts in tumorigenesis is most intriguing for functional research. Here we experimentally characterize an antisense mRNA asLZK overlapping the...
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| Veröffentlicht in: | Molecular biology (New York) 2008-08, Vol.42 (4), p.514-520 |
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| creator | Marakhonov, A. V. Baranova, A. V. Skoblov, M. Yu |
| description | Antisense regulation of gene expression is a widespread but poorly understood mechanism of gene expression regulation. The potential role of antisense transcripts in tumorigenesis is most intriguing for functional research. Here we experimentally characterize an antisense mRNA
asLZK
overlapping the human
MAP3K13/LZK
gene that is involved in the mitogenesis-related JNK/SAPK signal transduction pathway. According to the functional annotation of the human genome,
asLZK
transcript (LOC647276) is expressed at the relatively high level and overrepresented in tumor samples. To our surprise, experimental study of human
asLZK
revealed that this sequence is not expressed, but represents a silent pseudogene of ribosomal protein L4 encoding gene
RPL4
. This pseudogene resulted from relatively recent retroposition of
RPL4
mRNA into the first intron of
MAP3K13
gene and does not participate in the regulation of
MAP3K13
expression. This study stresses that, after initial in silico mapping efforts, experimental verification of the expression landscape is warranted. |
| doi_str_mv | 10.1134/S0026893308040055 |
| format | Article |
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asLZK
overlapping the human
MAP3K13/LZK
gene that is involved in the mitogenesis-related JNK/SAPK signal transduction pathway. According to the functional annotation of the human genome,
asLZK
transcript (LOC647276) is expressed at the relatively high level and overrepresented in tumor samples. To our surprise, experimental study of human
asLZK
revealed that this sequence is not expressed, but represents a silent pseudogene of ribosomal protein L4 encoding gene
RPL4
. This pseudogene resulted from relatively recent retroposition of
RPL4
mRNA into the first intron of
MAP3K13
gene and does not participate in the regulation of
MAP3K13
expression. This study stresses that, after initial in silico mapping efforts, experimental verification of the expression landscape is warranted.</description><identifier>ISSN: 0026-8933</identifier><identifier>EISSN: 1608-3245</identifier><identifier>DOI: 10.1134/S0026893308040055</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Antisense RNA ; Biochemistry ; Biomedical and Life Sciences ; Gene expression ; Gene mapping ; Gene regulation ; Genomes ; Genomics ; Genomics. Transcriptomics. Proteomics ; Human Genetics ; Life Sciences ; Molecular biology ; Proteins ; Ribosomal protein L4 ; Signal transduction ; Tumorigenesis</subject><ispartof>Molecular biology (New York), 2008-08, Vol.42 (4), p.514-520</ispartof><rights>MAIK Nauka 2008</rights><rights>MAIK Nauka 2008.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c326t-879c4a6ceb017a4933ecd01e45b761ad34587a55a90becf8722c0a848940f0a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0026893308040055$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0026893308040055$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Marakhonov, A. V.</creatorcontrib><creatorcontrib>Baranova, A. V.</creatorcontrib><creatorcontrib>Skoblov, M. Yu</creatorcontrib><title>Antisense regulation of human gene MAP3K13: True phenomenon or artifact?</title><title>Molecular biology (New York)</title><addtitle>Mol Biol</addtitle><description>Antisense regulation of gene expression is a widespread but poorly understood mechanism of gene expression regulation. The potential role of antisense transcripts in tumorigenesis is most intriguing for functional research. Here we experimentally characterize an antisense mRNA
asLZK
overlapping the human
MAP3K13/LZK
gene that is involved in the mitogenesis-related JNK/SAPK signal transduction pathway. According to the functional annotation of the human genome,
asLZK
transcript (LOC647276) is expressed at the relatively high level and overrepresented in tumor samples. To our surprise, experimental study of human
asLZK
revealed that this sequence is not expressed, but represents a silent pseudogene of ribosomal protein L4 encoding gene
RPL4
. This pseudogene resulted from relatively recent retroposition of
RPL4
mRNA into the first intron of
MAP3K13
gene and does not participate in the regulation of
MAP3K13
expression. This study stresses that, after initial in silico mapping efforts, experimental verification of the expression landscape is warranted.</description><subject>Antisense RNA</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Gene expression</subject><subject>Gene mapping</subject><subject>Gene regulation</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Genomics. Transcriptomics. Proteomics</subject><subject>Human Genetics</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Proteins</subject><subject>Ribosomal protein L4</subject><subject>Signal transduction</subject><subject>Tumorigenesis</subject><issn>0026-8933</issn><issn>1608-3245</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE9Lw0AQxRdRsFY_gLeg4C06-zcbL1KKWrGiYD2H7XbSpiSbupsc_PZuqQdRCjPM4f3e4zGEnFO4ppSLm3cApnTOOWgQAFIekAFVoFPOhDwkg62cbvVjchLCGoDGYQMyGbmuCugCJh6XfW26qnVJWyarvjEuWaLD5GX0xp8pv01mvsdks0LXNnEj5hPju6o0trs7JUelqQOe_dwh-Xi4n40n6fT18Wk8mqaWM9WlOsutMMriHGhmRCyEdgEUhZxnipoFF1JnRkqTwxxtqTPGLBgtdC6gBCP4kFztcje-_ewxdEVTBYt1bRy2fSgY5aBYBhG8_AOu29672K1gSknKNYNt3MU-iuZaMCV5HiG6g6xvQ_BYFhtfNcZ_FRSK7fuLf--PHrbzhMi6JfpfwXtN33_yg0k</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Marakhonov, A. 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V. ; Baranova, A. V. ; Skoblov, M. Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c326t-879c4a6ceb017a4933ecd01e45b761ad34587a55a90becf8722c0a848940f0a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antisense RNA</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Gene expression</topic><topic>Gene mapping</topic><topic>Gene regulation</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Genomics. Transcriptomics. Proteomics</topic><topic>Human Genetics</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>Proteins</topic><topic>Ribosomal protein L4</topic><topic>Signal transduction</topic><topic>Tumorigenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Marakhonov, A. V.</creatorcontrib><creatorcontrib>Baranova, A. 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Yu</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Molecular biology (New York)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Marakhonov, A. V.</au><au>Baranova, A. V.</au><au>Skoblov, M. Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antisense regulation of human gene MAP3K13: True phenomenon or artifact?</atitle><jtitle>Molecular biology (New York)</jtitle><stitle>Mol Biol</stitle><date>2008-08-01</date><risdate>2008</risdate><volume>42</volume><issue>4</issue><spage>514</spage><epage>520</epage><pages>514-520</pages><issn>0026-8933</issn><eissn>1608-3245</eissn><abstract>Antisense regulation of gene expression is a widespread but poorly understood mechanism of gene expression regulation. The potential role of antisense transcripts in tumorigenesis is most intriguing for functional research. Here we experimentally characterize an antisense mRNA
asLZK
overlapping the human
MAP3K13/LZK
gene that is involved in the mitogenesis-related JNK/SAPK signal transduction pathway. According to the functional annotation of the human genome,
asLZK
transcript (LOC647276) is expressed at the relatively high level and overrepresented in tumor samples. To our surprise, experimental study of human
asLZK
revealed that this sequence is not expressed, but represents a silent pseudogene of ribosomal protein L4 encoding gene
RPL4
. This pseudogene resulted from relatively recent retroposition of
RPL4
mRNA into the first intron of
MAP3K13
gene and does not participate in the regulation of
MAP3K13
expression. This study stresses that, after initial in silico mapping efforts, experimental verification of the expression landscape is warranted.</abstract><cop>Dordrecht</cop><pub>SP MAIK Nauka/Interperiodica</pub><doi>10.1134/S0026893308040055</doi><tpages>7</tpages></addata></record> |
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| subjects | Antisense RNA Biochemistry Biomedical and Life Sciences Gene expression Gene mapping Gene regulation Genomes Genomics Genomics. Transcriptomics. Proteomics Human Genetics Life Sciences Molecular biology Proteins Ribosomal protein L4 Signal transduction Tumorigenesis |
| title | Antisense regulation of human gene MAP3K13: True phenomenon or artifact? |
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