In vivo therapeutic efficacy of the Allium sativum ME in experimentally Echinococcus granulosus infected mice
•E. granulosus infected mice treated with A. sativum methanol extracts (ME) at 40 and 80 mL/L evoked high efficacy on treatment of hydatid cysts in the murine model.•The best therapeutic findings based on the total number of cysts and SEM finding were obtained following the administration of A. sati...
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| description | •E. granulosus infected mice treated with A. sativum methanol extracts (ME) at 40 and 80 mL/L evoked high efficacy on treatment of hydatid cysts in the murine model.•The best therapeutic findings based on the total number of cysts and SEM finding were obtained following the administration of A. sativum ME at 80 mL/L.•The hepatic variables (ALT, ALP and bilirubin) revealed less liver damage in A. sativum ME groups than in control groups.
Plant-based anthelmintics suggest an alternative treatment for cystic echinococcosis. The present study was conducted to evaluate the efficacy of methanolic extract (ME) of A. sativum (garlic) on the treatment of hydatid cysts in the murine model. After gas chromatography and mass spectrometry of prepared ME, sixty laboratory BALB/c mice were infected intraperitoneally by injection of 1500 viable protoscoleces. Five months after infection, the infected mice were allocated into six treatment groups, 1- Albendazole (100 mg/kg); 2- Allium sativum ME (10 mL/L); 3- A. sativum ME (20 mL/L); 4- A. sativum ME (40 mL/L); 5- A. sativum ME (80 mL/L) and 6- untreated control group.
After 30 days of daily treatment, the total number of cysts, size and weight of the largest cyst were significantly lower in three treated groups including A. sativum ME 80 mL/L, A. sativum ME 40 mL /L and albendazole in comparison to those of the control group (p |
| doi_str_mv | 10.1016/j.cimid.2018.10.001 |
| format | Article |
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Plant-based anthelmintics suggest an alternative treatment for cystic echinococcosis. The present study was conducted to evaluate the efficacy of methanolic extract (ME) of A. sativum (garlic) on the treatment of hydatid cysts in the murine model. After gas chromatography and mass spectrometry of prepared ME, sixty laboratory BALB/c mice were infected intraperitoneally by injection of 1500 viable protoscoleces. Five months after infection, the infected mice were allocated into six treatment groups, 1- Albendazole (100 mg/kg); 2- Allium sativum ME (10 mL/L); 3- A. sativum ME (20 mL/L); 4- A. sativum ME (40 mL/L); 5- A. sativum ME (80 mL/L) and 6- untreated control group.
After 30 days of daily treatment, the total number of cysts, size and weight of the largest cyst were significantly lower in three treated groups including A. sativum ME 80 mL/L, A. sativum ME 40 mL /L and albendazole in comparison to those of the control group (p < 0.05).
The activity of alanine amino transferase (ALT) enzyme, as well as bilirubin concentration were significantly lower in the mice treated with A. sativum ME 80, 40, 20 and 10 mL/L when compared to the control group. In addition, bilirubin concentration revealed significant decrease in A. sativum ME 10, 20 and 80 mL/L groups, when compared to the albendazole group. In conclusions, administration of A. sativum ME used at 40 and 80 mL/L concentrations might be beneficial in the treatment of CE due to anti-parasitic effects similar to albendazole but less hepatotoxic effects.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2018.10.001</identifier><identifier>PMID: 30396426</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Alanine ; Alanine Transaminase - blood ; Albendazole ; Allium sativum ; Animal models ; Animals ; Anthelmintic agents ; Anthelmintics - therapeutic use ; Aspartate Aminotransferases - blood ; Bilirubin ; Bilirubin - blood ; Biochemistry ; CD13 Antigens - blood ; Chemotherapy ; Cysts ; Disease Models, Animal ; Echinococcosis ; Echinococcosis, Hepatic - drug therapy ; Echinococcus granulosus - drug effects ; Experiments ; Female ; Garlic ; Garlic - chemistry ; Gas chromatography ; Gas Chromatography-Mass Spectrometry ; Hydatid cyst ; Liver - drug effects ; Mass spectrometry ; Mass spectroscopy ; Methanol ; Mice ; Mice, Inbred BALB C ; Onions ; Parasites ; Plant Extracts - pharmacology ; Rodents ; Sheep ; Sheep Diseases - parasitology ; Weight</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2018-10, Vol.60, p.23-27</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Oct 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-a2dad393cf96cd4dc167753fd6bc7c3fe7b3d8f8c3206c09d2bc9761fe44c1fd3</citedby><cites>FETCH-LOGICAL-c387t-a2dad393cf96cd4dc167753fd6bc7c3fe7b3d8f8c3206c09d2bc9761fe44c1fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147957118300833$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30396426$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haji Mohammadi, K.H.</creatorcontrib><creatorcontrib>Heidarpour, M.</creatorcontrib><creatorcontrib>Borji, H.</creatorcontrib><title>In vivo therapeutic efficacy of the Allium sativum ME in experimentally Echinococcus granulosus infected mice</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>•E. granulosus infected mice treated with A. sativum methanol extracts (ME) at 40 and 80 mL/L evoked high efficacy on treatment of hydatid cysts in the murine model.•The best therapeutic findings based on the total number of cysts and SEM finding were obtained following the administration of A. sativum ME at 80 mL/L.•The hepatic variables (ALT, ALP and bilirubin) revealed less liver damage in A. sativum ME groups than in control groups.
Plant-based anthelmintics suggest an alternative treatment for cystic echinococcosis. The present study was conducted to evaluate the efficacy of methanolic extract (ME) of A. sativum (garlic) on the treatment of hydatid cysts in the murine model. After gas chromatography and mass spectrometry of prepared ME, sixty laboratory BALB/c mice were infected intraperitoneally by injection of 1500 viable protoscoleces. Five months after infection, the infected mice were allocated into six treatment groups, 1- Albendazole (100 mg/kg); 2- Allium sativum ME (10 mL/L); 3- A. sativum ME (20 mL/L); 4- A. sativum ME (40 mL/L); 5- A. sativum ME (80 mL/L) and 6- untreated control group.
After 30 days of daily treatment, the total number of cysts, size and weight of the largest cyst were significantly lower in three treated groups including A. sativum ME 80 mL/L, A. sativum ME 40 mL /L and albendazole in comparison to those of the control group (p < 0.05).
The activity of alanine amino transferase (ALT) enzyme, as well as bilirubin concentration were significantly lower in the mice treated with A. sativum ME 80, 40, 20 and 10 mL/L when compared to the control group. In addition, bilirubin concentration revealed significant decrease in A. sativum ME 10, 20 and 80 mL/L groups, when compared to the albendazole group. In conclusions, administration of A. sativum ME used at 40 and 80 mL/L concentrations might be beneficial in the treatment of CE due to anti-parasitic effects similar to albendazole but less hepatotoxic effects.</description><subject>Alanine</subject><subject>Alanine Transaminase - blood</subject><subject>Albendazole</subject><subject>Allium sativum</subject><subject>Animal models</subject><subject>Animals</subject><subject>Anthelmintic agents</subject><subject>Anthelmintics - therapeutic use</subject><subject>Aspartate Aminotransferases - blood</subject><subject>Bilirubin</subject><subject>Bilirubin - blood</subject><subject>Biochemistry</subject><subject>CD13 Antigens - blood</subject><subject>Chemotherapy</subject><subject>Cysts</subject><subject>Disease Models, Animal</subject><subject>Echinococcosis</subject><subject>Echinococcosis, Hepatic - drug therapy</subject><subject>Echinococcus granulosus - drug effects</subject><subject>Experiments</subject><subject>Female</subject><subject>Garlic</subject><subject>Garlic - chemistry</subject><subject>Gas chromatography</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Hydatid cyst</subject><subject>Liver - drug effects</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Methanol</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Onions</subject><subject>Parasites</subject><subject>Plant Extracts - pharmacology</subject><subject>Rodents</subject><subject>Sheep</subject><subject>Sheep Diseases - parasitology</subject><subject>Weight</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFvEzEQhS0EomnhFyAhS1y4bGqvHXv3wKGqQqnUikt7tpzxmDratYO9GzX_HocUDhw4zejpmxn7PUI-cLbkjKvL7RLCGNyyZbyrypIx_ooseKe7hiulX5MF41I3_UrzM3JeypYx1nPJ35IzwUSvZKsWZLyNdB_2iU5PmO0O5ykARe8DWDjQ5I86vRqGMI-02Cnsa71f0xApPu8whxHjZIfhQNfwFGKCBDAX-iPbOA-p1DZEjzCho2MAfEfeeDsUfP9SL8jj1_XD9bfm7vvN7fXVXQOi01NjW2ed6AX4XoGTDrjSeiW8UxvQIDzqjXCd70C0TAHrXbuBXivuUUrg3okL8vm0d5fTzxnLZMZQAIfBRkxzMS2vDrCW97Kin_5Bt2nOsb6uUrLrV0y2olLiREFOpWT0Zlf_bvPBcGaOaZit-Z2GOaZxFGsaderjy-55M6L7O_PH_gp8OQFYzdgHzKZAwAjoQq6uGZfCfw_8Ammknac</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Haji Mohammadi, K.H.</creator><creator>Heidarpour, M.</creator><creator>Borji, H.</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>201810</creationdate><title>In vivo therapeutic efficacy of the Allium sativum ME in experimentally Echinococcus granulosus infected mice</title><author>Haji Mohammadi, K.H. ; Heidarpour, M. ; Borji, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-a2dad393cf96cd4dc167753fd6bc7c3fe7b3d8f8c3206c09d2bc9761fe44c1fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alanine</topic><topic>Alanine Transaminase - blood</topic><topic>Albendazole</topic><topic>Allium sativum</topic><topic>Animal models</topic><topic>Animals</topic><topic>Anthelmintic agents</topic><topic>Anthelmintics - therapeutic use</topic><topic>Aspartate Aminotransferases - blood</topic><topic>Bilirubin</topic><topic>Bilirubin - blood</topic><topic>Biochemistry</topic><topic>CD13 Antigens - blood</topic><topic>Chemotherapy</topic><topic>Cysts</topic><topic>Disease Models, Animal</topic><topic>Echinococcosis</topic><topic>Echinococcosis, Hepatic - drug therapy</topic><topic>Echinococcus granulosus - drug effects</topic><topic>Experiments</topic><topic>Female</topic><topic>Garlic</topic><topic>Garlic - chemistry</topic><topic>Gas chromatography</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Hydatid cyst</topic><topic>Liver - drug effects</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Methanol</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Onions</topic><topic>Parasites</topic><topic>Plant Extracts - pharmacology</topic><topic>Rodents</topic><topic>Sheep</topic><topic>Sheep Diseases - parasitology</topic><topic>Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haji Mohammadi, K.H.</creatorcontrib><creatorcontrib>Heidarpour, M.</creatorcontrib><creatorcontrib>Borji, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haji Mohammadi, K.H.</au><au>Heidarpour, M.</au><au>Borji, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo therapeutic efficacy of the Allium sativum ME in experimentally Echinococcus granulosus infected mice</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2018-10</date><risdate>2018</risdate><volume>60</volume><spage>23</spage><epage>27</epage><pages>23-27</pages><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>•E. granulosus infected mice treated with A. sativum methanol extracts (ME) at 40 and 80 mL/L evoked high efficacy on treatment of hydatid cysts in the murine model.•The best therapeutic findings based on the total number of cysts and SEM finding were obtained following the administration of A. sativum ME at 80 mL/L.•The hepatic variables (ALT, ALP and bilirubin) revealed less liver damage in A. sativum ME groups than in control groups.
Plant-based anthelmintics suggest an alternative treatment for cystic echinococcosis. The present study was conducted to evaluate the efficacy of methanolic extract (ME) of A. sativum (garlic) on the treatment of hydatid cysts in the murine model. After gas chromatography and mass spectrometry of prepared ME, sixty laboratory BALB/c mice were infected intraperitoneally by injection of 1500 viable protoscoleces. Five months after infection, the infected mice were allocated into six treatment groups, 1- Albendazole (100 mg/kg); 2- Allium sativum ME (10 mL/L); 3- A. sativum ME (20 mL/L); 4- A. sativum ME (40 mL/L); 5- A. sativum ME (80 mL/L) and 6- untreated control group.
After 30 days of daily treatment, the total number of cysts, size and weight of the largest cyst were significantly lower in three treated groups including A. sativum ME 80 mL/L, A. sativum ME 40 mL /L and albendazole in comparison to those of the control group (p < 0.05).
The activity of alanine amino transferase (ALT) enzyme, as well as bilirubin concentration were significantly lower in the mice treated with A. sativum ME 80, 40, 20 and 10 mL/L when compared to the control group. In addition, bilirubin concentration revealed significant decrease in A. sativum ME 10, 20 and 80 mL/L groups, when compared to the albendazole group. In conclusions, administration of A. sativum ME used at 40 and 80 mL/L concentrations might be beneficial in the treatment of CE due to anti-parasitic effects similar to albendazole but less hepatotoxic effects.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30396426</pmid><doi>10.1016/j.cimid.2018.10.001</doi><tpages>5</tpages></addata></record> |
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| subjects | Alanine Alanine Transaminase - blood Albendazole Allium sativum Animal models Animals Anthelmintic agents Anthelmintics - therapeutic use Aspartate Aminotransferases - blood Bilirubin Bilirubin - blood Biochemistry CD13 Antigens - blood Chemotherapy Cysts Disease Models, Animal Echinococcosis Echinococcosis, Hepatic - drug therapy Echinococcus granulosus - drug effects Experiments Female Garlic Garlic - chemistry Gas chromatography Gas Chromatography-Mass Spectrometry Hydatid cyst Liver - drug effects Mass spectrometry Mass spectroscopy Methanol Mice Mice, Inbred BALB C Onions Parasites Plant Extracts - pharmacology Rodents Sheep Sheep Diseases - parasitology Weight |
| title | In vivo therapeutic efficacy of the Allium sativum ME in experimentally Echinococcus granulosus infected mice |
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