Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge
•Heat-inactivated M.bovis reduces the lesions in goats naturally exposed to M.caprae.•Natural challenge is a realistic model to evaluate the efficacy of TB vaccines.•Goats are an experimental model for TB vaccine development. Caprine TB causes chronic disease with severe economic and health conseque...
Gespeichert in:
| Veröffentlicht in: | Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2018-10, Vol.60, p.28-34 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 34 |
|---|---|
| container_issue | |
| container_start_page | 28 |
| container_title | Comparative immunology, microbiology and infectious diseases |
| container_volume | 60 |
| creator | Roy, Álvaro Risalde, María A. Bezos, Javier Casal, Carmen Romero, Beatriz Sevilla, Iker Díez-Guerrier, Alberto Rodríguez-Bertos, Antonio Domínguez, Mercedes Garrido, Joseba Gortázar, Christian Domínguez, Lucas |
| description | •Heat-inactivated M.bovis reduces the lesions in goats naturally exposed to M.caprae.•Natural challenge is a realistic model to evaluate the efficacy of TB vaccines.•Goats are an experimental model for TB vaccine development.
Caprine TB causes chronic disease with severe economic and health consequences. We assessed the effect of intramuscularly administered heat-inactivated Mycobacterium bovis (M. bovis) on 20 kid goats (10 vaccinated, 10 controls), naturally exposed to M. caprae through close contact with infected goats. At necropsy, visible TB-compatible lesions were recorded in all animals with the exception of 1 control and 2 vaccinated goats. The median of the total lesion score was 9 (IQR 3–13.5) and 5 (IQR 3–6.75) in control and vaccinated goats, respectively (median lesion reduction 44.4%, p = 0.224). The lung lesions of the vaccinated goats were restricted to the caudal lobes, while 6 controls had additional lung lobes affected (p = 0.01). The median lung lesion score reduction in vaccinated goats was 100%; however, this reduction was not significant (p = 0.124), possibly due to the low sample size. Regarding the abdomen, only one vaccinated goat presented visible lesions compared to three goats in the control group. The results provide further evidence of the potential of heat-inactivated M. bovis for controlling TB in different host species, including ruminants. |
| doi_str_mv | 10.1016/j.cimid.2018.09.006 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2130301555</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0147957118300638</els_id><sourcerecordid>2130301555</sourcerecordid><originalsourceid>FETCH-LOGICAL-c387t-63b221e13ee9f54a108ba9b35ef63cd9ab79c32a215930b98186039527e6dd3</originalsourceid><addsrcrecordid>eNp90cFu1DAQBmALUdGl8ARIyBIXLgkeO4njAwdUFYpUVKlwtxx70vWSxIvtLOrb12VbDhw4-fLNjGd-Qt4Aq4FB92FXWz97V3MGfc1UzVj3jGygl30FXSefkw2DRlaqlXBKXqa0Y4wpaOAFORVMqK7hckP8DaZ9WBLSMNLbYHKiOVC_5GjmNdl1MpEejLV-MdmHhf72eUu3aHL1008TOvrtzobB2IzRrzMdwsEnahZHi1-jmajdmuKWW3xFTkYzJXz9-J6R758vfpxfVlfXX76ef7qqrOhlrjoxcA4IAlGNbWOA9YNRg2hx7IR1ygxSWcENh1YJNqge-q5s03KJnXPijLw_dt3H8GvFlPXsk8VpMguGNWkOZXcGbdsW-u4fugtrXMrfimp61UhoeFHiqGwMKUUc9T762cQ7DUw_5KB3-k8O-iEHzZQuOZSqt4-912FG97fm6fAFfDwCLKc4eIw6WY-LRecj2qxd8P8dcA_L8Jqx</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2148947142</pqid></control><display><type>article</type><title>Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Roy, Álvaro ; Risalde, María A. ; Bezos, Javier ; Casal, Carmen ; Romero, Beatriz ; Sevilla, Iker ; Díez-Guerrier, Alberto ; Rodríguez-Bertos, Antonio ; Domínguez, Mercedes ; Garrido, Joseba ; Gortázar, Christian ; Domínguez, Lucas</creator><creatorcontrib>Roy, Álvaro ; Risalde, María A. ; Bezos, Javier ; Casal, Carmen ; Romero, Beatriz ; Sevilla, Iker ; Díez-Guerrier, Alberto ; Rodríguez-Bertos, Antonio ; Domínguez, Mercedes ; Garrido, Joseba ; Gortázar, Christian ; Domínguez, Lucas</creatorcontrib><description>•Heat-inactivated M.bovis reduces the lesions in goats naturally exposed to M.caprae.•Natural challenge is a realistic model to evaluate the efficacy of TB vaccines.•Goats are an experimental model for TB vaccine development.
Caprine TB causes chronic disease with severe economic and health consequences. We assessed the effect of intramuscularly administered heat-inactivated Mycobacterium bovis (M. bovis) on 20 kid goats (10 vaccinated, 10 controls), naturally exposed to M. caprae through close contact with infected goats. At necropsy, visible TB-compatible lesions were recorded in all animals with the exception of 1 control and 2 vaccinated goats. The median of the total lesion score was 9 (IQR 3–13.5) and 5 (IQR 3–6.75) in control and vaccinated goats, respectively (median lesion reduction 44.4%, p = 0.224). The lung lesions of the vaccinated goats were restricted to the caudal lobes, while 6 controls had additional lung lobes affected (p = 0.01). The median lung lesion score reduction in vaccinated goats was 100%; however, this reduction was not significant (p = 0.124), possibly due to the low sample size. Regarding the abdomen, only one vaccinated goat presented visible lesions compared to three goats in the control group. The results provide further evidence of the potential of heat-inactivated M. bovis for controlling TB in different host species, including ruminants.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2018.09.006</identifier><identifier>PMID: 30396427</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Caprinae ; Chronic illnesses ; Female ; Goat Diseases - microbiology ; Goat Diseases - prevention & control ; Goats ; Gram-positive bacteria ; Heat ; Heat-Inactivated vaccine ; Hot Temperature ; Immunization ; Injections, Intramuscular ; Lesions ; Lobes ; Lung - microbiology ; Lung - pathology ; Lungs ; Mycobacterium bovis ; Mycobacterium bovis - immunology ; Natural infection ; Necropsy ; Reduction ; Tuberculosis ; Tuberculosis - prevention & control ; Tuberculosis Vaccines - administration & dosage ; Tuberculosis Vaccines - therapeutic use ; Vaccination ; Vaccination - veterinary</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2018-10, Vol.60, p.28-34</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Oct 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-63b221e13ee9f54a108ba9b35ef63cd9ab79c32a215930b98186039527e6dd3</citedby><cites>FETCH-LOGICAL-c387t-63b221e13ee9f54a108ba9b35ef63cd9ab79c32a215930b98186039527e6dd3</cites><orcidid>0000-0003-0012-4006</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0147957118300638$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30396427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Roy, Álvaro</creatorcontrib><creatorcontrib>Risalde, María A.</creatorcontrib><creatorcontrib>Bezos, Javier</creatorcontrib><creatorcontrib>Casal, Carmen</creatorcontrib><creatorcontrib>Romero, Beatriz</creatorcontrib><creatorcontrib>Sevilla, Iker</creatorcontrib><creatorcontrib>Díez-Guerrier, Alberto</creatorcontrib><creatorcontrib>Rodríguez-Bertos, Antonio</creatorcontrib><creatorcontrib>Domínguez, Mercedes</creatorcontrib><creatorcontrib>Garrido, Joseba</creatorcontrib><creatorcontrib>Gortázar, Christian</creatorcontrib><creatorcontrib>Domínguez, Lucas</creatorcontrib><title>Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>•Heat-inactivated M.bovis reduces the lesions in goats naturally exposed to M.caprae.•Natural challenge is a realistic model to evaluate the efficacy of TB vaccines.•Goats are an experimental model for TB vaccine development.
Caprine TB causes chronic disease with severe economic and health consequences. We assessed the effect of intramuscularly administered heat-inactivated Mycobacterium bovis (M. bovis) on 20 kid goats (10 vaccinated, 10 controls), naturally exposed to M. caprae through close contact with infected goats. At necropsy, visible TB-compatible lesions were recorded in all animals with the exception of 1 control and 2 vaccinated goats. The median of the total lesion score was 9 (IQR 3–13.5) and 5 (IQR 3–6.75) in control and vaccinated goats, respectively (median lesion reduction 44.4%, p = 0.224). The lung lesions of the vaccinated goats were restricted to the caudal lobes, while 6 controls had additional lung lobes affected (p = 0.01). The median lung lesion score reduction in vaccinated goats was 100%; however, this reduction was not significant (p = 0.124), possibly due to the low sample size. Regarding the abdomen, only one vaccinated goat presented visible lesions compared to three goats in the control group. The results provide further evidence of the potential of heat-inactivated M. bovis for controlling TB in different host species, including ruminants.</description><subject>Animals</subject><subject>Caprinae</subject><subject>Chronic illnesses</subject><subject>Female</subject><subject>Goat Diseases - microbiology</subject><subject>Goat Diseases - prevention & control</subject><subject>Goats</subject><subject>Gram-positive bacteria</subject><subject>Heat</subject><subject>Heat-Inactivated vaccine</subject><subject>Hot Temperature</subject><subject>Immunization</subject><subject>Injections, Intramuscular</subject><subject>Lesions</subject><subject>Lobes</subject><subject>Lung - microbiology</subject><subject>Lung - pathology</subject><subject>Lungs</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - immunology</subject><subject>Natural infection</subject><subject>Necropsy</subject><subject>Reduction</subject><subject>Tuberculosis</subject><subject>Tuberculosis - prevention & control</subject><subject>Tuberculosis Vaccines - administration & dosage</subject><subject>Tuberculosis Vaccines - therapeutic use</subject><subject>Vaccination</subject><subject>Vaccination - veterinary</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90cFu1DAQBmALUdGl8ARIyBIXLgkeO4njAwdUFYpUVKlwtxx70vWSxIvtLOrb12VbDhw4-fLNjGd-Qt4Aq4FB92FXWz97V3MGfc1UzVj3jGygl30FXSefkw2DRlaqlXBKXqa0Y4wpaOAFORVMqK7hckP8DaZ9WBLSMNLbYHKiOVC_5GjmNdl1MpEejLV-MdmHhf72eUu3aHL1008TOvrtzobB2IzRrzMdwsEnahZHi1-jmajdmuKWW3xFTkYzJXz9-J6R758vfpxfVlfXX76ef7qqrOhlrjoxcA4IAlGNbWOA9YNRg2hx7IR1ygxSWcENh1YJNqge-q5s03KJnXPijLw_dt3H8GvFlPXsk8VpMguGNWkOZXcGbdsW-u4fugtrXMrfimp61UhoeFHiqGwMKUUc9T762cQ7DUw_5KB3-k8O-iEHzZQuOZSqt4-912FG97fm6fAFfDwCLKc4eIw6WY-LRecj2qxd8P8dcA_L8Jqx</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Roy, Álvaro</creator><creator>Risalde, María A.</creator><creator>Bezos, Javier</creator><creator>Casal, Carmen</creator><creator>Romero, Beatriz</creator><creator>Sevilla, Iker</creator><creator>Díez-Guerrier, Alberto</creator><creator>Rodríguez-Bertos, Antonio</creator><creator>Domínguez, Mercedes</creator><creator>Garrido, Joseba</creator><creator>Gortázar, Christian</creator><creator>Domínguez, Lucas</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T2</scope><scope>7T5</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0012-4006</orcidid></search><sort><creationdate>201810</creationdate><title>Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge</title><author>Roy, Álvaro ; Risalde, María A. ; Bezos, Javier ; Casal, Carmen ; Romero, Beatriz ; Sevilla, Iker ; Díez-Guerrier, Alberto ; Rodríguez-Bertos, Antonio ; Domínguez, Mercedes ; Garrido, Joseba ; Gortázar, Christian ; Domínguez, Lucas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c387t-63b221e13ee9f54a108ba9b35ef63cd9ab79c32a215930b98186039527e6dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Caprinae</topic><topic>Chronic illnesses</topic><topic>Female</topic><topic>Goat Diseases - microbiology</topic><topic>Goat Diseases - prevention & control</topic><topic>Goats</topic><topic>Gram-positive bacteria</topic><topic>Heat</topic><topic>Heat-Inactivated vaccine</topic><topic>Hot Temperature</topic><topic>Immunization</topic><topic>Injections, Intramuscular</topic><topic>Lesions</topic><topic>Lobes</topic><topic>Lung - microbiology</topic><topic>Lung - pathology</topic><topic>Lungs</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - immunology</topic><topic>Natural infection</topic><topic>Necropsy</topic><topic>Reduction</topic><topic>Tuberculosis</topic><topic>Tuberculosis - prevention & control</topic><topic>Tuberculosis Vaccines - administration & dosage</topic><topic>Tuberculosis Vaccines - therapeutic use</topic><topic>Vaccination</topic><topic>Vaccination - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Roy, Álvaro</creatorcontrib><creatorcontrib>Risalde, María A.</creatorcontrib><creatorcontrib>Bezos, Javier</creatorcontrib><creatorcontrib>Casal, Carmen</creatorcontrib><creatorcontrib>Romero, Beatriz</creatorcontrib><creatorcontrib>Sevilla, Iker</creatorcontrib><creatorcontrib>Díez-Guerrier, Alberto</creatorcontrib><creatorcontrib>Rodríguez-Bertos, Antonio</creatorcontrib><creatorcontrib>Domínguez, Mercedes</creatorcontrib><creatorcontrib>Garrido, Joseba</creatorcontrib><creatorcontrib>Gortázar, Christian</creatorcontrib><creatorcontrib>Domínguez, Lucas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Roy, Álvaro</au><au>Risalde, María A.</au><au>Bezos, Javier</au><au>Casal, Carmen</au><au>Romero, Beatriz</au><au>Sevilla, Iker</au><au>Díez-Guerrier, Alberto</au><au>Rodríguez-Bertos, Antonio</au><au>Domínguez, Mercedes</au><au>Garrido, Joseba</au><au>Gortázar, Christian</au><au>Domínguez, Lucas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2018-10</date><risdate>2018</risdate><volume>60</volume><spage>28</spage><epage>34</epage><pages>28-34</pages><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>•Heat-inactivated M.bovis reduces the lesions in goats naturally exposed to M.caprae.•Natural challenge is a realistic model to evaluate the efficacy of TB vaccines.•Goats are an experimental model for TB vaccine development.
Caprine TB causes chronic disease with severe economic and health consequences. We assessed the effect of intramuscularly administered heat-inactivated Mycobacterium bovis (M. bovis) on 20 kid goats (10 vaccinated, 10 controls), naturally exposed to M. caprae through close contact with infected goats. At necropsy, visible TB-compatible lesions were recorded in all animals with the exception of 1 control and 2 vaccinated goats. The median of the total lesion score was 9 (IQR 3–13.5) and 5 (IQR 3–6.75) in control and vaccinated goats, respectively (median lesion reduction 44.4%, p = 0.224). The lung lesions of the vaccinated goats were restricted to the caudal lobes, while 6 controls had additional lung lobes affected (p = 0.01). The median lung lesion score reduction in vaccinated goats was 100%; however, this reduction was not significant (p = 0.124), possibly due to the low sample size. Regarding the abdomen, only one vaccinated goat presented visible lesions compared to three goats in the control group. The results provide further evidence of the potential of heat-inactivated M. bovis for controlling TB in different host species, including ruminants.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30396427</pmid><doi>10.1016/j.cimid.2018.09.006</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0012-4006</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0147-9571 |
| ispartof | Comparative immunology, microbiology and infectious diseases, 2018-10, Vol.60, p.28-34 |
| issn | 0147-9571 1878-1667 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2130301555 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Caprinae Chronic illnesses Female Goat Diseases - microbiology Goat Diseases - prevention & control Goats Gram-positive bacteria Heat Heat-Inactivated vaccine Hot Temperature Immunization Injections, Intramuscular Lesions Lobes Lung - microbiology Lung - pathology Lungs Mycobacterium bovis Mycobacterium bovis - immunology Natural infection Necropsy Reduction Tuberculosis Tuberculosis - prevention & control Tuberculosis Vaccines - administration & dosage Tuberculosis Vaccines - therapeutic use Vaccination Vaccination - veterinary |
| title | Response of goats to intramuscular vaccination with heat-killed Mycobacterium bovis and natural challenge |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T15%3A33%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Response%20of%20goats%20to%20intramuscular%20vaccination%20with%20heat-killed%20Mycobacterium%20bovis%20and%20natural%20challenge&rft.jtitle=Comparative%20immunology,%20microbiology%20and%20infectious%20diseases&rft.au=Roy,%20%C3%81lvaro&rft.date=2018-10&rft.volume=60&rft.spage=28&rft.epage=34&rft.pages=28-34&rft.issn=0147-9571&rft.eissn=1878-1667&rft_id=info:doi/10.1016/j.cimid.2018.09.006&rft_dat=%3Cproquest_cross%3E2130301555%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2148947142&rft_id=info:pmid/30396427&rft_els_id=S0147957118300638&rfr_iscdi=true |