Evaluation of a new freezing protocol containing 20% dimethyl sulphoxide concentration to cryopreserve human ovarian tissue
Could a modification in the ovarian tissue freezing protocol improve follicle survival after cryopreservation and xenotransplantation? Ovarian tissue was used from 13 adult patients, frozen either with our original protocol, or a modified version involving a higher concentration of dimethyl sulphoxi...
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| Veröffentlicht in: | Reproductive biomedicine online 2018-12, Vol.37 (6), p.653-665 |
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| container_title | Reproductive biomedicine online |
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| creator | Gallardo, Miguel Paulini, Fernanda Corral, Ariadna Balcerzyk, Marcin Lucci, Carolina M. Ambroise, Jérôme Merola, Marta Fernandez-maza, Laura Risco, Rámon Dolmans, Marie-Madeleine Amorim, Christiani A. |
| description | Could a modification in the ovarian tissue freezing protocol improve follicle survival after cryopreservation and xenotransplantation?
Ovarian tissue was used from 13 adult patients, frozen either with our original protocol, or a modified version involving a higher concentration of dimethyl sulphoxide (DMSO), larger volume of cryopreservation solution and lower seeding temperature. After thawing, the ovarian fragments were xenotransplanted to six mice with severe combined immunodeficiency (SCID) for 3 weeks.
The proportion of primordial follicles decreased, and the proportion of growing follicles increased significantly (all P < 0.01) after cryopreservation and xenografting compared with fresh controls for both protocols. Follicle density, development, ultrastructure and function were similar between treatments.
This study showed that, although the higher DMSO concentration did not improve survival of preantral follicles, it did not seem to induce any major toxicity in the follicle population either. |
| doi_str_mv | 10.1016/j.rbmo.2018.09.012 |
| format | Article |
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Ovarian tissue was used from 13 adult patients, frozen either with our original protocol, or a modified version involving a higher concentration of dimethyl sulphoxide (DMSO), larger volume of cryopreservation solution and lower seeding temperature. After thawing, the ovarian fragments were xenotransplanted to six mice with severe combined immunodeficiency (SCID) for 3 weeks.
The proportion of primordial follicles decreased, and the proportion of growing follicles increased significantly (all P < 0.01) after cryopreservation and xenografting compared with fresh controls for both protocols. Follicle density, development, ultrastructure and function were similar between treatments.
This study showed that, although the higher DMSO concentration did not improve survival of preantral follicles, it did not seem to induce any major toxicity in the follicle population either.</description><identifier>ISSN: 1472-6483</identifier><identifier>EISSN: 1472-6491</identifier><identifier>DOI: 10.1016/j.rbmo.2018.09.012</identifier><identifier>PMID: 30391164</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Cancer patients ; Fertility preservation ; Freezing protocol ; Ovarian tissue cryopreservation ; Preantral follicles ; Xenotransplantation</subject><ispartof>Reproductive biomedicine online, 2018-12, Vol.37 (6), p.653-665</ispartof><rights>2018 Reproductive Healthcare Ltd.</rights><rights>Copyright © 2018 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-3b2eee1d2f917d913d62c78e7ad1b99726d2c4c9dac35f5d3a83199420fefd513</citedby><cites>FETCH-LOGICAL-c356t-3b2eee1d2f917d913d62c78e7ad1b99726d2c4c9dac35f5d3a83199420fefd513</cites><orcidid>0000-0002-6331-3026 ; 0000-0001-6030-7416 ; 0000-0001-7241-7401 ; 0000-0001-5995-6590 ; 0000-0003-1794-0368</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1472648318304887$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30391164$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gallardo, Miguel</creatorcontrib><creatorcontrib>Paulini, Fernanda</creatorcontrib><creatorcontrib>Corral, Ariadna</creatorcontrib><creatorcontrib>Balcerzyk, Marcin</creatorcontrib><creatorcontrib>Lucci, Carolina M.</creatorcontrib><creatorcontrib>Ambroise, Jérôme</creatorcontrib><creatorcontrib>Merola, Marta</creatorcontrib><creatorcontrib>Fernandez-maza, Laura</creatorcontrib><creatorcontrib>Risco, Rámon</creatorcontrib><creatorcontrib>Dolmans, Marie-Madeleine</creatorcontrib><creatorcontrib>Amorim, Christiani A.</creatorcontrib><title>Evaluation of a new freezing protocol containing 20% dimethyl sulphoxide concentration to cryopreserve human ovarian tissue</title><title>Reproductive biomedicine online</title><addtitle>Reprod Biomed Online</addtitle><description>Could a modification in the ovarian tissue freezing protocol improve follicle survival after cryopreservation and xenotransplantation?
Ovarian tissue was used from 13 adult patients, frozen either with our original protocol, or a modified version involving a higher concentration of dimethyl sulphoxide (DMSO), larger volume of cryopreservation solution and lower seeding temperature. After thawing, the ovarian fragments were xenotransplanted to six mice with severe combined immunodeficiency (SCID) for 3 weeks.
The proportion of primordial follicles decreased, and the proportion of growing follicles increased significantly (all P < 0.01) after cryopreservation and xenografting compared with fresh controls for both protocols. Follicle density, development, ultrastructure and function were similar between treatments.
This study showed that, although the higher DMSO concentration did not improve survival of preantral follicles, it did not seem to induce any major toxicity in the follicle population either.</description><subject>Cancer patients</subject><subject>Fertility preservation</subject><subject>Freezing protocol</subject><subject>Ovarian tissue cryopreservation</subject><subject>Preantral follicles</subject><subject>Xenotransplantation</subject><issn>1472-6483</issn><issn>1472-6491</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rGzEQhkVpqB23f6CHokshF2800np3Bb2UkC8w9JKchSzN1jK7K1fSunXy56PFSY49zTA88w7zEPIVWAEMqstdETa9LziDpmCyYMA_kDmUNV9WpYSP730jZuQ8xh3LIGvEJzITTEiAqpyT5-uD7kadnB-ob6mmA_6lbUB8csNvug8-eeM7avyQtBumGWffqXU9pu2xo3Hs9lv_z1mcEINDCqes5KkJR78PGDEckG7HXucLBx1crsnFOOJnctbqLuKX17ogjzfXD1d3y_Wv2_urn-ulEasqLcWGIyJY3kqorQRhK27qBmttYSNlzSvLTWmk1ZlvV1boRoCUJWcttnYFYkEuTrn5nT8jxqR6Fw12nR7Qj1FxEIytMlhmlJ9QE3yMAVu1D67X4aiAqUm62qlJupqkKyZVlp6Xvr3mj5se7fvKm-UM_DgBmL88OAwqGodZl3UBTVLWu__lvwCNtpYV</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Gallardo, Miguel</creator><creator>Paulini, Fernanda</creator><creator>Corral, Ariadna</creator><creator>Balcerzyk, Marcin</creator><creator>Lucci, Carolina M.</creator><creator>Ambroise, Jérôme</creator><creator>Merola, Marta</creator><creator>Fernandez-maza, Laura</creator><creator>Risco, Rámon</creator><creator>Dolmans, Marie-Madeleine</creator><creator>Amorim, Christiani A.</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6331-3026</orcidid><orcidid>https://orcid.org/0000-0001-6030-7416</orcidid><orcidid>https://orcid.org/0000-0001-7241-7401</orcidid><orcidid>https://orcid.org/0000-0001-5995-6590</orcidid><orcidid>https://orcid.org/0000-0003-1794-0368</orcidid></search><sort><creationdate>201812</creationdate><title>Evaluation of a new freezing protocol containing 20% dimethyl sulphoxide concentration to cryopreserve human ovarian tissue</title><author>Gallardo, Miguel ; Paulini, Fernanda ; Corral, Ariadna ; Balcerzyk, Marcin ; Lucci, Carolina M. ; Ambroise, Jérôme ; Merola, Marta ; Fernandez-maza, Laura ; Risco, Rámon ; Dolmans, Marie-Madeleine ; Amorim, Christiani A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-3b2eee1d2f917d913d62c78e7ad1b99726d2c4c9dac35f5d3a83199420fefd513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cancer patients</topic><topic>Fertility preservation</topic><topic>Freezing protocol</topic><topic>Ovarian tissue cryopreservation</topic><topic>Preantral follicles</topic><topic>Xenotransplantation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gallardo, Miguel</creatorcontrib><creatorcontrib>Paulini, Fernanda</creatorcontrib><creatorcontrib>Corral, Ariadna</creatorcontrib><creatorcontrib>Balcerzyk, Marcin</creatorcontrib><creatorcontrib>Lucci, Carolina M.</creatorcontrib><creatorcontrib>Ambroise, Jérôme</creatorcontrib><creatorcontrib>Merola, Marta</creatorcontrib><creatorcontrib>Fernandez-maza, Laura</creatorcontrib><creatorcontrib>Risco, Rámon</creatorcontrib><creatorcontrib>Dolmans, Marie-Madeleine</creatorcontrib><creatorcontrib>Amorim, Christiani A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive biomedicine online</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gallardo, Miguel</au><au>Paulini, Fernanda</au><au>Corral, Ariadna</au><au>Balcerzyk, Marcin</au><au>Lucci, Carolina M.</au><au>Ambroise, Jérôme</au><au>Merola, Marta</au><au>Fernandez-maza, Laura</au><au>Risco, Rámon</au><au>Dolmans, Marie-Madeleine</au><au>Amorim, Christiani A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of a new freezing protocol containing 20% dimethyl sulphoxide concentration to cryopreserve human ovarian tissue</atitle><jtitle>Reproductive biomedicine online</jtitle><addtitle>Reprod Biomed Online</addtitle><date>2018-12</date><risdate>2018</risdate><volume>37</volume><issue>6</issue><spage>653</spage><epage>665</epage><pages>653-665</pages><issn>1472-6483</issn><eissn>1472-6491</eissn><abstract>Could a modification in the ovarian tissue freezing protocol improve follicle survival after cryopreservation and xenotransplantation?
Ovarian tissue was used from 13 adult patients, frozen either with our original protocol, or a modified version involving a higher concentration of dimethyl sulphoxide (DMSO), larger volume of cryopreservation solution and lower seeding temperature. After thawing, the ovarian fragments were xenotransplanted to six mice with severe combined immunodeficiency (SCID) for 3 weeks.
The proportion of primordial follicles decreased, and the proportion of growing follicles increased significantly (all P < 0.01) after cryopreservation and xenografting compared with fresh controls for both protocols. Follicle density, development, ultrastructure and function were similar between treatments.
This study showed that, although the higher DMSO concentration did not improve survival of preantral follicles, it did not seem to induce any major toxicity in the follicle population either.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>30391164</pmid><doi>10.1016/j.rbmo.2018.09.012</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-6331-3026</orcidid><orcidid>https://orcid.org/0000-0001-6030-7416</orcidid><orcidid>https://orcid.org/0000-0001-7241-7401</orcidid><orcidid>https://orcid.org/0000-0001-5995-6590</orcidid><orcidid>https://orcid.org/0000-0003-1794-0368</orcidid></addata></record> |
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| source | Elsevier ScienceDirect Journals |
| subjects | Cancer patients Fertility preservation Freezing protocol Ovarian tissue cryopreservation Preantral follicles Xenotransplantation |
| title | Evaluation of a new freezing protocol containing 20% dimethyl sulphoxide concentration to cryopreserve human ovarian tissue |
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