Consensus Amplification and Novel Multiplex Sequencing Method for S Segment Species Identification of 47 Viruses of the Orthobunyavirus, Phlebovirus, and Nairovirus Genera of the Family Bunyaviridae

A reverse transcription-PCR (RT-PCR) assay was designed, according to previously determined and newly derived genetic data, to target S genomic segments of 47 viruses, including 29 arthropod-borne human pathogens, of the family BUNYAVIRIDAE: The analytical sensitivity of the presented assay was eval...

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Veröffentlicht in:	Journal of Clinical Microbiology 2009-08, Vol.47 (8), p.2398-2404
Hauptverfasser:	Lambert, Amy J, Lanciotti, Robert S
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Bunyaviridae Bunyaviridae Infections - diagnosis Bunyaviridae Infections - virology Bunyavirus DNA Primers - genetics Fundamental and applied biological sciences. Psychology Humans Microbiology Miscellaneous Molecular Sequence Data Nairovirus Nairovirus - classification Nairovirus - genetics Orthobunyavirus Orthobunyavirus - classification Orthobunyavirus - genetics Phlebovirus Phlebovirus - classification Phlebovirus - genetics Polymerase Chain Reaction - methods RNA, Viral - genetics Sensitivity and Specificity Sequence Analysis, DNA - methods Virology
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