Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes
Contents Growth differentiation factor 9 (GDF‐9) and bone morphogenetic protein 15 (BMP‐15) have pivotal roles in oocyte development in many species, therefore the aim was to investigate these factors during in vitro maturation (IVM) of canine oocytes. Canine cumulus oocytes complexes (COCs) were cu...
Gespeichert in:
| Veröffentlicht in: | Reproduction in domestic animals 2019-02, Vol.54 (2), p.373-380 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 380 |
|---|---|
| container_issue | 2 |
| container_start_page | 373 |
| container_title | Reproduction in domestic animals |
| container_volume | 54 |
| creator | Garcia, Pablo Aspee, Karla Ramirez, Georges Dettleff, Phillip Palomino, Jaime Peralta, Oscar A. Parraguez, Víctor H. De los Reyes, Monica |
| description | Contents
Growth differentiation factor 9 (GDF‐9) and bone morphogenetic protein 15 (BMP‐15) have pivotal roles in oocyte development in many species, therefore the aim was to investigate these factors during in vitro maturation (IVM) of canine oocytes. Canine cumulus oocytes complexes (COCs) were cultured in six groups for 72 hr in a supplemented TCM199‐Hepes medium as (a) Control group; (b) GDF‐9 antibody (Ab); (c) BMP‐15 Ab; (d) recombinant human (rh) GDF‐9; (e) rh BMP‐15 or (f) rh BMP‐15 and GDF‐9. Data were evaluated by ANOVA. The Abs against GDF‐9 or BMP‐15 had a negative impact on meiotic development. Higher (p |
| doi_str_mv | 10.1111/rda.13371 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2129538377</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2129538377</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3531-acbd927b94f0c298dcdeb69df3ca6b8d874c03b7128a2f348eb7d4b0bdc9883d3</originalsourceid><addsrcrecordid>eNp1kc1O3TAQRi1EBbe0C16gssSmLAJ2nMT2EtHSIiFVqmAd-WcMRol9azu9um9ft4EuKtWb8eLM0cx8CJ1SckHru0xWXVDGOD1AG9ox2ZCe0UO0IZINzcAHcYze5vxMCO0F50fomBEmBKN0g3a3wU0LBAM4OvyY4q48YeudgwSheFV8DNgpU2LCEqtgsY4B8BzT9ik-QoDiDd6mWMAHTHtc6fr56UuKeFZlSauhuo0KvnbGaPYF8jv0xqkpw_uXeoIebj7fX39t7r59ub2-umsMqzs0ymgrW65l54hppbDGgh6kdcyoQQsreGcI05y2QrWOdQI0t50m2hpZN7TsBH1cvXXGHwvkMs4-G5gmFSAueWxpK3smGOcVPfsHfY5LCnW6SnFBaUt6WanzlTIp5pzAjdvkZ5X2IyXj7zTGmsb4J43KfngxLnoG-5d8PX8FLldg5yfY_980fv90tSp_Ac2tlPs</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2178112059</pqid></control><display><type>article</type><title>Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Garcia, Pablo ; Aspee, Karla ; Ramirez, Georges ; Dettleff, Phillip ; Palomino, Jaime ; Peralta, Oscar A. ; Parraguez, Víctor H. ; De los Reyes, Monica</creator><creatorcontrib>Garcia, Pablo ; Aspee, Karla ; Ramirez, Georges ; Dettleff, Phillip ; Palomino, Jaime ; Peralta, Oscar A. ; Parraguez, Víctor H. ; De los Reyes, Monica</creatorcontrib><description>Contents
Growth differentiation factor 9 (GDF‐9) and bone morphogenetic protein 15 (BMP‐15) have pivotal roles in oocyte development in many species, therefore the aim was to investigate these factors during in vitro maturation (IVM) of canine oocytes. Canine cumulus oocytes complexes (COCs) were cultured in six groups for 72 hr in a supplemented TCM199‐Hepes medium as (a) Control group; (b) GDF‐9 antibody (Ab); (c) BMP‐15 Ab; (d) recombinant human (rh) GDF‐9; (e) rh BMP‐15 or (f) rh BMP‐15 and GDF‐9. Data were evaluated by ANOVA. The Abs against GDF‐9 or BMP‐15 had a negative impact on meiotic development. Higher (p < 0.05) number of oocytes was arrested at GVBD stage when they were incubated with either GDF‐9 Ab (64.4 ± 2.1%) or BMP‐15 Ab (67.2%± 4.9%) in comparison to those in control group (32.4 ± 7.8%). In contrast, more (p < 0.05) oocytes in control group reached MI (37.4 ± 1.3%) and MII stages (10.2 ± 2.1%) comparing to those groups with GDF‐9 Ab (23.1 ± 4.7% MI; 0.0% MII) or BMP‐15 Ab (16.4 ± 2.4%MI; 5.9% ± 2.1 MII). Higher rates (p < 0.05) of oocytes in control group stayed still arrested at GV (19.9 ± 8.6%) in comparison to those cultured with either rhGDF‐9 (3.7 ± 0.4%) or rhBMP‐15 (10.9 ± 0.7%). However, there were no differences in MII rates between oocytes cultured with GDF‐9 (14.7 ± 3.1) and BMP‐15 (7.8 ± 2.5) separately. But, more oocytes (p < 0.05) reached the MII stage (20.5 ± 3.8%) compared to those exposed to each protein separately and to the control group. These results suggest that these proteins likely contribute to the meiotic development in dogs.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.13371</identifier><identifier>PMID: 30388311</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies - pharmacology ; Bone Morphogenetic Protein 15 - pharmacology ; Bone morphogenetic proteins ; Cells, Cultured ; Differentiation ; dog gametes ; Dogs ; Female ; Growth differentiation factor 9 ; Growth Differentiation Factor 9 - pharmacology ; growth factors ; Humans ; in vitro ; In Vitro Oocyte Maturation Techniques - veterinary ; Maturation ; Meiosis ; meiotic development ; Oocytes ; Oocytes - drug effects ; Oocytes - physiology ; Oogenesis ; Proteins ; Recombinant Proteins - pharmacology ; Variance analysis</subject><ispartof>Reproduction in domestic animals, 2019-02, Vol.54 (2), p.373-380</ispartof><rights>2018 Blackwell Verlag GmbH</rights><rights>2018 Blackwell Verlag GmbH.</rights><rights>Copyright © 2019 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3531-acbd927b94f0c298dcdeb69df3ca6b8d874c03b7128a2f348eb7d4b0bdc9883d3</citedby><cites>FETCH-LOGICAL-c3531-acbd927b94f0c298dcdeb69df3ca6b8d874c03b7128a2f348eb7d4b0bdc9883d3</cites><orcidid>0000-0003-2040-4902 ; 0000-0003-1665-436X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.13371$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.13371$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30388311$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia, Pablo</creatorcontrib><creatorcontrib>Aspee, Karla</creatorcontrib><creatorcontrib>Ramirez, Georges</creatorcontrib><creatorcontrib>Dettleff, Phillip</creatorcontrib><creatorcontrib>Palomino, Jaime</creatorcontrib><creatorcontrib>Peralta, Oscar A.</creatorcontrib><creatorcontrib>Parraguez, Víctor H.</creatorcontrib><creatorcontrib>De los Reyes, Monica</creatorcontrib><title>Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
Growth differentiation factor 9 (GDF‐9) and bone morphogenetic protein 15 (BMP‐15) have pivotal roles in oocyte development in many species, therefore the aim was to investigate these factors during in vitro maturation (IVM) of canine oocytes. Canine cumulus oocytes complexes (COCs) were cultured in six groups for 72 hr in a supplemented TCM199‐Hepes medium as (a) Control group; (b) GDF‐9 antibody (Ab); (c) BMP‐15 Ab; (d) recombinant human (rh) GDF‐9; (e) rh BMP‐15 or (f) rh BMP‐15 and GDF‐9. Data were evaluated by ANOVA. The Abs against GDF‐9 or BMP‐15 had a negative impact on meiotic development. Higher (p < 0.05) number of oocytes was arrested at GVBD stage when they were incubated with either GDF‐9 Ab (64.4 ± 2.1%) or BMP‐15 Ab (67.2%± 4.9%) in comparison to those in control group (32.4 ± 7.8%). In contrast, more (p < 0.05) oocytes in control group reached MI (37.4 ± 1.3%) and MII stages (10.2 ± 2.1%) comparing to those groups with GDF‐9 Ab (23.1 ± 4.7% MI; 0.0% MII) or BMP‐15 Ab (16.4 ± 2.4%MI; 5.9% ± 2.1 MII). Higher rates (p < 0.05) of oocytes in control group stayed still arrested at GV (19.9 ± 8.6%) in comparison to those cultured with either rhGDF‐9 (3.7 ± 0.4%) or rhBMP‐15 (10.9 ± 0.7%). However, there were no differences in MII rates between oocytes cultured with GDF‐9 (14.7 ± 3.1) and BMP‐15 (7.8 ± 2.5) separately. But, more oocytes (p < 0.05) reached the MII stage (20.5 ± 3.8%) compared to those exposed to each protein separately and to the control group. These results suggest that these proteins likely contribute to the meiotic development in dogs.</description><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Bone Morphogenetic Protein 15 - pharmacology</subject><subject>Bone morphogenetic proteins</subject><subject>Cells, Cultured</subject><subject>Differentiation</subject><subject>dog gametes</subject><subject>Dogs</subject><subject>Female</subject><subject>Growth differentiation factor 9</subject><subject>Growth Differentiation Factor 9 - pharmacology</subject><subject>growth factors</subject><subject>Humans</subject><subject>in vitro</subject><subject>In Vitro Oocyte Maturation Techniques - veterinary</subject><subject>Maturation</subject><subject>Meiosis</subject><subject>meiotic development</subject><subject>Oocytes</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - physiology</subject><subject>Oogenesis</subject><subject>Proteins</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Variance analysis</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1O3TAQRi1EBbe0C16gssSmLAJ2nMT2EtHSIiFVqmAd-WcMRol9azu9um9ft4EuKtWb8eLM0cx8CJ1SckHru0xWXVDGOD1AG9ox2ZCe0UO0IZINzcAHcYze5vxMCO0F50fomBEmBKN0g3a3wU0LBAM4OvyY4q48YeudgwSheFV8DNgpU2LCEqtgsY4B8BzT9ik-QoDiDd6mWMAHTHtc6fr56UuKeFZlSauhuo0KvnbGaPYF8jv0xqkpw_uXeoIebj7fX39t7r59ub2-umsMqzs0ymgrW65l54hppbDGgh6kdcyoQQsreGcI05y2QrWOdQI0t50m2hpZN7TsBH1cvXXGHwvkMs4-G5gmFSAueWxpK3smGOcVPfsHfY5LCnW6SnFBaUt6WanzlTIp5pzAjdvkZ5X2IyXj7zTGmsb4J43KfngxLnoG-5d8PX8FLldg5yfY_980fv90tSp_Ac2tlPs</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Garcia, Pablo</creator><creator>Aspee, Karla</creator><creator>Ramirez, Georges</creator><creator>Dettleff, Phillip</creator><creator>Palomino, Jaime</creator><creator>Peralta, Oscar A.</creator><creator>Parraguez, Víctor H.</creator><creator>De los Reyes, Monica</creator><general>Blackwell Publishing Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2040-4902</orcidid><orcidid>https://orcid.org/0000-0003-1665-436X</orcidid></search><sort><creationdate>201902</creationdate><title>Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes</title><author>Garcia, Pablo ; Aspee, Karla ; Ramirez, Georges ; Dettleff, Phillip ; Palomino, Jaime ; Peralta, Oscar A. ; Parraguez, Víctor H. ; De los Reyes, Monica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3531-acbd927b94f0c298dcdeb69df3ca6b8d874c03b7128a2f348eb7d4b0bdc9883d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antibodies - pharmacology</topic><topic>Bone Morphogenetic Protein 15 - pharmacology</topic><topic>Bone morphogenetic proteins</topic><topic>Cells, Cultured</topic><topic>Differentiation</topic><topic>dog gametes</topic><topic>Dogs</topic><topic>Female</topic><topic>Growth differentiation factor 9</topic><topic>Growth Differentiation Factor 9 - pharmacology</topic><topic>growth factors</topic><topic>Humans</topic><topic>in vitro</topic><topic>In Vitro Oocyte Maturation Techniques - veterinary</topic><topic>Maturation</topic><topic>Meiosis</topic><topic>meiotic development</topic><topic>Oocytes</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - physiology</topic><topic>Oogenesis</topic><topic>Proteins</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia, Pablo</creatorcontrib><creatorcontrib>Aspee, Karla</creatorcontrib><creatorcontrib>Ramirez, Georges</creatorcontrib><creatorcontrib>Dettleff, Phillip</creatorcontrib><creatorcontrib>Palomino, Jaime</creatorcontrib><creatorcontrib>Peralta, Oscar A.</creatorcontrib><creatorcontrib>Parraguez, Víctor H.</creatorcontrib><creatorcontrib>De los Reyes, Monica</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia, Pablo</au><au>Aspee, Karla</au><au>Ramirez, Georges</au><au>Dettleff, Phillip</au><au>Palomino, Jaime</au><au>Peralta, Oscar A.</au><au>Parraguez, Víctor H.</au><au>De los Reyes, Monica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2019-02</date><risdate>2019</risdate><volume>54</volume><issue>2</issue><spage>373</spage><epage>380</epage><pages>373-380</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
Growth differentiation factor 9 (GDF‐9) and bone morphogenetic protein 15 (BMP‐15) have pivotal roles in oocyte development in many species, therefore the aim was to investigate these factors during in vitro maturation (IVM) of canine oocytes. Canine cumulus oocytes complexes (COCs) were cultured in six groups for 72 hr in a supplemented TCM199‐Hepes medium as (a) Control group; (b) GDF‐9 antibody (Ab); (c) BMP‐15 Ab; (d) recombinant human (rh) GDF‐9; (e) rh BMP‐15 or (f) rh BMP‐15 and GDF‐9. Data were evaluated by ANOVA. The Abs against GDF‐9 or BMP‐15 had a negative impact on meiotic development. Higher (p < 0.05) number of oocytes was arrested at GVBD stage when they were incubated with either GDF‐9 Ab (64.4 ± 2.1%) or BMP‐15 Ab (67.2%± 4.9%) in comparison to those in control group (32.4 ± 7.8%). In contrast, more (p < 0.05) oocytes in control group reached MI (37.4 ± 1.3%) and MII stages (10.2 ± 2.1%) comparing to those groups with GDF‐9 Ab (23.1 ± 4.7% MI; 0.0% MII) or BMP‐15 Ab (16.4 ± 2.4%MI; 5.9% ± 2.1 MII). Higher rates (p < 0.05) of oocytes in control group stayed still arrested at GV (19.9 ± 8.6%) in comparison to those cultured with either rhGDF‐9 (3.7 ± 0.4%) or rhBMP‐15 (10.9 ± 0.7%). However, there were no differences in MII rates between oocytes cultured with GDF‐9 (14.7 ± 3.1) and BMP‐15 (7.8 ± 2.5) separately. But, more oocytes (p < 0.05) reached the MII stage (20.5 ± 3.8%) compared to those exposed to each protein separately and to the control group. These results suggest that these proteins likely contribute to the meiotic development in dogs.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>30388311</pmid><doi>10.1111/rda.13371</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2040-4902</orcidid><orcidid>https://orcid.org/0000-0003-1665-436X</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0936-6768 |
| ispartof | Reproduction in domestic animals, 2019-02, Vol.54 (2), p.373-380 |
| issn | 0936-6768 1439-0531 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2129538377 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Animals Antibodies - pharmacology Bone Morphogenetic Protein 15 - pharmacology Bone morphogenetic proteins Cells, Cultured Differentiation dog gametes Dogs Female Growth differentiation factor 9 Growth Differentiation Factor 9 - pharmacology growth factors Humans in vitro In Vitro Oocyte Maturation Techniques - veterinary Maturation Meiosis meiotic development Oocytes Oocytes - drug effects Oocytes - physiology Oogenesis Proteins Recombinant Proteins - pharmacology Variance analysis |
| title | Influence of growth differentiation factor 9 and bone morphogenetic protein 15 on in vitro maturation of canine oocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T13%3A27%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20growth%20differentiation%20factor%209%20and%20bone%20morphogenetic%20protein%2015%20on%20in%20vitro%20maturation%20of%20canine%20oocytes&rft.jtitle=Reproduction%20in%20domestic%20animals&rft.au=Garcia,%20Pablo&rft.date=2019-02&rft.volume=54&rft.issue=2&rft.spage=373&rft.epage=380&rft.pages=373-380&rft.issn=0936-6768&rft.eissn=1439-0531&rft_id=info:doi/10.1111/rda.13371&rft_dat=%3Cproquest_cross%3E2129538377%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2178112059&rft_id=info:pmid/30388311&rfr_iscdi=true |