MiRNA‐199a‐5p positively regulated RANKL‐induced osteoclast differentiation by target Mafb protein

MicroRNAs are involved in osteoclast differentiation. Although miR‐199a‐5p plays an important role in many different systems and diseases, its function during osteoclastogenesis remains unclear. In this study, we investigated the function and the target gene of miR‐199a‐5p in osteoclast differentiat...

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Veröffentlicht in:Journal of cellular biochemistry 2019-05, Vol.120 (5), p.7024-7031
Hauptverfasser: Guo, Kai, Zhang, Dawei, Wu, Haining, Zhu, Qingsheng, Yang, Chongfei, Zhu, Jinyu
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container_issue 5
container_start_page 7024
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creator Guo, Kai
Zhang, Dawei
Wu, Haining
Zhu, Qingsheng
Yang, Chongfei
Zhu, Jinyu
description MicroRNAs are involved in osteoclast differentiation. Although miR‐199a‐5p plays an important role in many different systems and diseases, its function during osteoclastogenesis remains unclear. In this study, we investigated the function and the target gene of miR‐199a‐5p in osteoclast differentiation. The in vitro data showed that miR‐199a‐5p was significantly upregulated after the stimulation by receptor activator of nuclear factor kappa‐B ligand in macrophages and RAW 264.7 cells. After transfection of miR‐199a‐5p mimic, the messenger RNA expression level of nuclear factor of activated T‐cells cytoplasmic 1, tartrate‐resistant acid phosphatase (TRAP), and receptor activator of nuclear factor kappa‐B was significantly increased in RAW 264.7 cells and the number of TRAP‐positive cells was also increased. MiR‐199a‐5p inhibitor showed the complete opposite outcome which brought additional proof to our finding. Overexpression of miR‐199a‐5p led to downregulation of Mafb protein. The luciferase activity was obviously repressed when WT‐pGL3‐Mafb and miR‐199a‐5p mimics were cotransfected into 293 T cells and the inhibitors cotransfected demonstrated reverse result. MiR‐199a‐5p overexpressed during osteoclast differentiation and positively regulated osteoclast formation in vitro by target Mafb. Mir‐199a‐5p positively regulated receptor activator of nuclear factor kappa‐B ligand (RANKL)‐induced osteoclast.
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Although miR‐199a‐5p plays an important role in many different systems and diseases, its function during osteoclastogenesis remains unclear. In this study, we investigated the function and the target gene of miR‐199a‐5p in osteoclast differentiation. The in vitro data showed that miR‐199a‐5p was significantly upregulated after the stimulation by receptor activator of nuclear factor kappa‐B ligand in macrophages and RAW 264.7 cells. After transfection of miR‐199a‐5p mimic, the messenger RNA expression level of nuclear factor of activated T‐cells cytoplasmic 1, tartrate‐resistant acid phosphatase (TRAP), and receptor activator of nuclear factor kappa‐B was significantly increased in RAW 264.7 cells and the number of TRAP‐positive cells was also increased. MiR‐199a‐5p inhibitor showed the complete opposite outcome which brought additional proof to our finding. Overexpression of miR‐199a‐5p led to downregulation of Mafb protein. The luciferase activity was obviously repressed when WT‐pGL3‐Mafb and miR‐199a‐5p mimics were cotransfected into 293 T cells and the inhibitors cotransfected demonstrated reverse result. MiR‐199a‐5p overexpressed during osteoclast differentiation and positively regulated osteoclast formation in vitro by target Mafb. 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The luciferase activity was obviously repressed when WT‐pGL3‐Mafb and miR‐199a‐5p mimics were cotransfected into 293 T cells and the inhibitors cotransfected demonstrated reverse result. MiR‐199a‐5p overexpressed during osteoclast differentiation and positively regulated osteoclast formation in vitro by target Mafb. 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subjects Acid phosphatase
Acid phosphatase (tartrate-resistant)
Acid resistance
Biocompatibility
Biomedical materials
Chromosome 5
Differentiation
Gene expression
Lymphocytes
Lymphocytes T
Macrophages
MAFB protein
miRNA
Mir‐199a‐5p
nuclear factor of activated T‐cells cytoplasmic 1 (NFATc1)
osteoclast
Osteoclastogenesis
Proteins
receptor activator of nuclear factor kappa‐B ligand (RANKL)
Ribonucleic acid
RNA
TRANCE protein
Transfection
title MiRNA‐199a‐5p positively regulated RANKL‐induced osteoclast differentiation by target Mafb protein
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