A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives
Abstract Background Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their pai...
Gespeichert in:
| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2019-02, Vol.113 (2), p.101-104 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 104 |
|---|---|
| container_issue | 2 |
| container_start_page | 101 |
| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| container_volume | 113 |
| creator | Parkhouse, R Michael E Carpio, Arturo Campoverde, Alfredo Sastre, Patricia Rojas, Glenda Harrison, Leslie J S Cortez, Maria Milagros |
| description | Abstract
Background
Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.
Methods
Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
Results
The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
Conclusions
The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis. |
| doi_str_mv | 10.1093/trstmh/try116 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2127949031</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/trstmh/try116</oup_id><sourcerecordid>2127949031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c365t-b506ffc1c9a9791edc757cd0793254ec0676922b118fd536f892122893b34b413</originalsourceid><addsrcrecordid>eNqFkU9P3DAQxa0KVLbQI9fKRw4E_CdO4iNChVZC6qWcI8ced40SO_U4VPsd-NDNsrQ99vSkmd97I80j5JyzK860vC4Zy7RdZcd5845seNd2lVRMHpENY1JVWjB5Qj4gPjEmFFf6PTmRTHZStPWGvNzQKbngAzg6mgLZjNSP6Rc1iGZ3SRcM8QdFyMt0SX3KtGyBZjMHR4ODWFanNSWkSJOn22UykZro6JCeQwRqd1iChWwTBqQhvrrNgBAt7A3ejAh0XrclPAOekePXycc3PSWPd5-_336pHr7df729eaisbFSpBsUa7y232uhWc3C2Va11rNVSqBosa9pGCzFw3nmnZOM7LbgQnZaDrIeay1Nyccidc_q5AJZ-CmhhHE2EtGC_0q2uNZN7tDqgNifEDL6fc5hM3vWc9fsC-kMB_aGAlf_0Fr0ME7i_9J-P_7udlvk_Wb8BIuyTRQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2127949031</pqid></control><display><type>article</type><title>A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives</title><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Parkhouse, R Michael E ; Carpio, Arturo ; Campoverde, Alfredo ; Sastre, Patricia ; Rojas, Glenda ; Harrison, Leslie J S ; Cortez, Maria Milagros</creator><creatorcontrib>Parkhouse, R Michael E ; Carpio, Arturo ; Campoverde, Alfredo ; Sastre, Patricia ; Rojas, Glenda ; Harrison, Leslie J S ; Cortez, Maria Milagros</creatorcontrib><description>Abstract
Background
Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.
Methods
Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
Results
The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
Conclusions
The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1093/trstmh/try116</identifier><identifier>PMID: 30383274</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Biological Assay - methods ; Blood - parasitology ; Cattle ; Cysticercosis - blood ; Cysticercosis - diagnosis ; Cysticercus - isolation & purification ; Deoxycholic Acid ; Dithiothreitol ; Ecuador - epidemiology ; False Positive Reactions ; Female ; Humans ; Male</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 2019-02, Vol.113 (2), p.101-104</ispartof><rights>The Author(s) 2018. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c365t-b506ffc1c9a9791edc757cd0793254ec0676922b118fd536f892122893b34b413</citedby><cites>FETCH-LOGICAL-c365t-b506ffc1c9a9791edc757cd0793254ec0676922b118fd536f892122893b34b413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30383274$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parkhouse, R Michael E</creatorcontrib><creatorcontrib>Carpio, Arturo</creatorcontrib><creatorcontrib>Campoverde, Alfredo</creatorcontrib><creatorcontrib>Sastre, Patricia</creatorcontrib><creatorcontrib>Rojas, Glenda</creatorcontrib><creatorcontrib>Harrison, Leslie J S</creatorcontrib><creatorcontrib>Cortez, Maria Milagros</creatorcontrib><title>A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>Abstract
Background
Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.
Methods
Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
Results
The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
Conclusions
The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.</description><subject>Animals</subject><subject>Biological Assay - methods</subject><subject>Blood - parasitology</subject><subject>Cattle</subject><subject>Cysticercosis - blood</subject><subject>Cysticercosis - diagnosis</subject><subject>Cysticercus - isolation & purification</subject><subject>Deoxycholic Acid</subject><subject>Dithiothreitol</subject><subject>Ecuador - epidemiology</subject><subject>False Positive Reactions</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9P3DAQxa0KVLbQI9fKRw4E_CdO4iNChVZC6qWcI8ced40SO_U4VPsd-NDNsrQ99vSkmd97I80j5JyzK860vC4Zy7RdZcd5845seNd2lVRMHpENY1JVWjB5Qj4gPjEmFFf6PTmRTHZStPWGvNzQKbngAzg6mgLZjNSP6Rc1iGZ3SRcM8QdFyMt0SX3KtGyBZjMHR4ODWFanNSWkSJOn22UykZro6JCeQwRqd1iChWwTBqQhvrrNgBAt7A3ejAh0XrclPAOekePXycc3PSWPd5-_336pHr7df729eaisbFSpBsUa7y232uhWc3C2Va11rNVSqBosa9pGCzFw3nmnZOM7LbgQnZaDrIeay1Nyccidc_q5AJZ-CmhhHE2EtGC_0q2uNZN7tDqgNifEDL6fc5hM3vWc9fsC-kMB_aGAlf_0Fr0ME7i_9J-P_7udlvk_Wb8BIuyTRQ</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Parkhouse, R Michael E</creator><creator>Carpio, Arturo</creator><creator>Campoverde, Alfredo</creator><creator>Sastre, Patricia</creator><creator>Rojas, Glenda</creator><creator>Harrison, Leslie J S</creator><creator>Cortez, Maria Milagros</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190201</creationdate><title>A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives</title><author>Parkhouse, R Michael E ; Carpio, Arturo ; Campoverde, Alfredo ; Sastre, Patricia ; Rojas, Glenda ; Harrison, Leslie J S ; Cortez, Maria Milagros</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-b506ffc1c9a9791edc757cd0793254ec0676922b118fd536f892122893b34b413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biological Assay - methods</topic><topic>Blood - parasitology</topic><topic>Cattle</topic><topic>Cysticercosis - blood</topic><topic>Cysticercosis - diagnosis</topic><topic>Cysticercus - isolation & purification</topic><topic>Deoxycholic Acid</topic><topic>Dithiothreitol</topic><topic>Ecuador - epidemiology</topic><topic>False Positive Reactions</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parkhouse, R Michael E</creatorcontrib><creatorcontrib>Carpio, Arturo</creatorcontrib><creatorcontrib>Campoverde, Alfredo</creatorcontrib><creatorcontrib>Sastre, Patricia</creatorcontrib><creatorcontrib>Rojas, Glenda</creatorcontrib><creatorcontrib>Harrison, Leslie J S</creatorcontrib><creatorcontrib>Cortez, Maria Milagros</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parkhouse, R Michael E</au><au>Carpio, Arturo</au><au>Campoverde, Alfredo</au><au>Sastre, Patricia</au><au>Rojas, Glenda</au><au>Harrison, Leslie J S</au><au>Cortez, Maria Milagros</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>113</volume><issue>2</issue><spage>101</spage><epage>104</epage><pages>101-104</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>Abstract
Background
Previously we reported the use of a monoclonal antibody–based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases.
Methods
Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol.
Results
The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA.
Conclusions
The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30383274</pmid><doi>10.1093/trstmh/try116</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 2019-02, Vol.113 (2), p.101-104 |
| issn | 0035-9203 1878-3503 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2127949031 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Animals Biological Assay - methods Blood - parasitology Cattle Cysticercosis - blood Cysticercosis - diagnosis Cysticercus - isolation & purification Deoxycholic Acid Dithiothreitol Ecuador - epidemiology False Positive Reactions Female Humans Male |
| title | A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T07%3A31%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20modified%20lateral%20flow%20assay,%20using%20serum,%20for%20the%20rapid%20identification%20of%20human%20and%20bovine%20cysticercosis%20in%20the%20absence%20of%20false%20positives&rft.jtitle=Transactions%20of%20the%20Royal%20Society%20of%20Tropical%20Medicine%20and%20Hygiene&rft.au=Parkhouse,%20R%20Michael%20E&rft.date=2019-02-01&rft.volume=113&rft.issue=2&rft.spage=101&rft.epage=104&rft.pages=101-104&rft.issn=0035-9203&rft.eissn=1878-3503&rft_id=info:doi/10.1093/trstmh/try116&rft_dat=%3Cproquest_cross%3E2127949031%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2127949031&rft_id=info:pmid/30383274&rft_oup_id=10.1093/trstmh/try116&rfr_iscdi=true |