Whole cell protein profiling characterization of Corynebacterium diphtheriae clinical isolates collected from various infections
Corynebacterium diphtheriae can cause various infections such as diphtheria, wound infections, septic arthritis, bacteraemia and endocarditis. Different virulence properties of the isolates might be related to different virulence factors expressed by the isolates. The objective of this study was to...
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| Veröffentlicht in: | Medycyna doświadczalna i mikrobiologia 2016, Vol.68 (3-4), p.161-166 |
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| creator | Zasada, Aleksandra A Rzeczkowska, Magdalena Wołkowicz, Tomasz Formińska, Kamila Zacharczuk, Katarzyna Wolaniuk, Natalia Paduch, Olga Badell, Edgar Januszkiewicz, Aleksandra Guiso, Nicole |
| description | Corynebacterium diphtheriae can cause various infections such as diphtheria, wound infections, septic arthritis, bacteraemia and endocarditis. Different virulence properties of the isolates might be related to different virulence factors expressed by the isolates. The objective of this study was to explore whether whole cell protein profiling might be useful in prediction of pathogenic properties of C. diphtheriae isolates.
C. disphtheriae isolates collected from diphtheria, invasive and local infections and from asymptomatic carriers in Poland, France, New Caledonia and Canada in 1950-2014 were investigated using whole cell protein profile analysis.
All the examined isolates were divided into two clades: A and B with similarity about 47%, but clade B was represented by only one isolate. The clade A was divided in two subclades A.I NS .II with similarity 53,2% and then into four groups: A.Ia, A.Ib, A.Ic and A.Id. The comparative analysis did not distinguish clearly toxigenic and nontoxigenic isolates as well as invasive and noninvasive isolates.
Whole cell protein profile analysis of C. diphtheria exhibits good concordance with other genotyping methods but this method is not able to distinguish clearly invasive from non-invasive isolates. |
| format | Article |
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C. disphtheriae isolates collected from diphtheria, invasive and local infections and from asymptomatic carriers in Poland, France, New Caledonia and Canada in 1950-2014 were investigated using whole cell protein profile analysis.
All the examined isolates were divided into two clades: A and B with similarity about 47%, but clade B was represented by only one isolate. The clade A was divided in two subclades A.I NS .II with similarity 53,2% and then into four groups: A.Ia, A.Ib, A.Ic and A.Id. The comparative analysis did not distinguish clearly toxigenic and nontoxigenic isolates as well as invasive and noninvasive isolates.
Whole cell protein profile analysis of C. diphtheria exhibits good concordance with other genotyping methods but this method is not able to distinguish clearly invasive from non-invasive isolates.</description><identifier>ISSN: 0025-8601</identifier><identifier>PMID: 30376615</identifier><language>pol</language><publisher>Poland</publisher><subject>Bacterial Proteins ; Bacterial Typing Techniques ; Corynebacterium diphtheriae - classification ; Corynebacterium diphtheriae - pathogenicity ; Diphtheria - genetics ; Diphtheria - metabolism ; Electrophoresis, Polyacrylamide Gel ; Humans</subject><ispartof>Medycyna doświadczalna i mikrobiologia, 2016, Vol.68 (3-4), p.161-166</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30376615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zasada, Aleksandra A</creatorcontrib><creatorcontrib>Rzeczkowska, Magdalena</creatorcontrib><creatorcontrib>Wołkowicz, Tomasz</creatorcontrib><creatorcontrib>Formińska, Kamila</creatorcontrib><creatorcontrib>Zacharczuk, Katarzyna</creatorcontrib><creatorcontrib>Wolaniuk, Natalia</creatorcontrib><creatorcontrib>Paduch, Olga</creatorcontrib><creatorcontrib>Badell, Edgar</creatorcontrib><creatorcontrib>Januszkiewicz, Aleksandra</creatorcontrib><creatorcontrib>Guiso, Nicole</creatorcontrib><title>Whole cell protein profiling characterization of Corynebacterium diphtheriae clinical isolates collected from various infections</title><title>Medycyna doświadczalna i mikrobiologia</title><addtitle>Med Dosw Mikrobiol</addtitle><description>Corynebacterium diphtheriae can cause various infections such as diphtheria, wound infections, septic arthritis, bacteraemia and endocarditis. Different virulence properties of the isolates might be related to different virulence factors expressed by the isolates. The objective of this study was to explore whether whole cell protein profiling might be useful in prediction of pathogenic properties of C. diphtheriae isolates.
C. disphtheriae isolates collected from diphtheria, invasive and local infections and from asymptomatic carriers in Poland, France, New Caledonia and Canada in 1950-2014 were investigated using whole cell protein profile analysis.
All the examined isolates were divided into two clades: A and B with similarity about 47%, but clade B was represented by only one isolate. The clade A was divided in two subclades A.I NS .II with similarity 53,2% and then into four groups: A.Ia, A.Ib, A.Ic and A.Id. The comparative analysis did not distinguish clearly toxigenic and nontoxigenic isolates as well as invasive and noninvasive isolates.
Whole cell protein profile analysis of C. diphtheria exhibits good concordance with other genotyping methods but this method is not able to distinguish clearly invasive from non-invasive isolates.</description><subject>Bacterial Proteins</subject><subject>Bacterial Typing Techniques</subject><subject>Corynebacterium diphtheriae - classification</subject><subject>Corynebacterium diphtheriae - pathogenicity</subject><subject>Diphtheria - genetics</subject><subject>Diphtheria - metabolism</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Humans</subject><issn>0025-8601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1UE1PwzAMzQHEprG_gHLkUiltSNYd0cSXhMRlEsfKTWwalDYlaZHGiZ9Opg1fnv30_OTnC7YUolJFrUW5YOuUPkUuXSuh9BVbSCE3WpdqyX7fu-CRG_SejzFM6IYjkvNu-OCmgwhmwuh-YHJh4IH4LsTDgO2Jnntu3dhNXe4h2-QtZ8Bzl4KHCRM3wXvMUssphp5_Q3RhTtwNlNnsmK7ZJYFPuD7jiu0fH_a75-L17elld_9ajEqrom03WNVSGgIDEqGsSjStJWsrpbdiS1UJmbgjAmtJkQSi0uZJEaraarlityfbHO5rxjQ1vUvH1DBgPqipymqj1bYWIktvztK57dE2Y3Q9xEPz_zT5B9FRbog</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Zasada, Aleksandra A</creator><creator>Rzeczkowska, Magdalena</creator><creator>Wołkowicz, Tomasz</creator><creator>Formińska, Kamila</creator><creator>Zacharczuk, Katarzyna</creator><creator>Wolaniuk, Natalia</creator><creator>Paduch, Olga</creator><creator>Badell, Edgar</creator><creator>Januszkiewicz, Aleksandra</creator><creator>Guiso, Nicole</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>2016</creationdate><title>Whole cell protein profiling characterization of Corynebacterium diphtheriae clinical isolates collected from various infections</title><author>Zasada, Aleksandra A ; Rzeczkowska, Magdalena ; Wołkowicz, Tomasz ; Formińska, Kamila ; Zacharczuk, Katarzyna ; Wolaniuk, Natalia ; Paduch, Olga ; Badell, Edgar ; Januszkiewicz, Aleksandra ; Guiso, Nicole</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p565-bb7e2833cfaca3ea121ecbdfdd256909f21aecb4ffaddf5f3aff1dffa5fe58d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>pol</language><creationdate>2016</creationdate><topic>Bacterial Proteins</topic><topic>Bacterial Typing Techniques</topic><topic>Corynebacterium diphtheriae - classification</topic><topic>Corynebacterium diphtheriae - pathogenicity</topic><topic>Diphtheria - genetics</topic><topic>Diphtheria - metabolism</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Humans</topic><toplevel>online_resources</toplevel><creatorcontrib>Zasada, Aleksandra A</creatorcontrib><creatorcontrib>Rzeczkowska, Magdalena</creatorcontrib><creatorcontrib>Wołkowicz, Tomasz</creatorcontrib><creatorcontrib>Formińska, Kamila</creatorcontrib><creatorcontrib>Zacharczuk, Katarzyna</creatorcontrib><creatorcontrib>Wolaniuk, Natalia</creatorcontrib><creatorcontrib>Paduch, Olga</creatorcontrib><creatorcontrib>Badell, Edgar</creatorcontrib><creatorcontrib>Januszkiewicz, Aleksandra</creatorcontrib><creatorcontrib>Guiso, Nicole</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Medycyna doświadczalna i mikrobiologia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zasada, Aleksandra A</au><au>Rzeczkowska, Magdalena</au><au>Wołkowicz, Tomasz</au><au>Formińska, Kamila</au><au>Zacharczuk, Katarzyna</au><au>Wolaniuk, Natalia</au><au>Paduch, Olga</au><au>Badell, Edgar</au><au>Januszkiewicz, Aleksandra</au><au>Guiso, Nicole</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole cell protein profiling characterization of Corynebacterium diphtheriae clinical isolates collected from various infections</atitle><jtitle>Medycyna doświadczalna i mikrobiologia</jtitle><addtitle>Med Dosw Mikrobiol</addtitle><date>2016</date><risdate>2016</risdate><volume>68</volume><issue>3-4</issue><spage>161</spage><epage>166</epage><pages>161-166</pages><issn>0025-8601</issn><abstract>Corynebacterium diphtheriae can cause various infections such as diphtheria, wound infections, septic arthritis, bacteraemia and endocarditis. Different virulence properties of the isolates might be related to different virulence factors expressed by the isolates. The objective of this study was to explore whether whole cell protein profiling might be useful in prediction of pathogenic properties of C. diphtheriae isolates.
C. disphtheriae isolates collected from diphtheria, invasive and local infections and from asymptomatic carriers in Poland, France, New Caledonia and Canada in 1950-2014 were investigated using whole cell protein profile analysis.
All the examined isolates were divided into two clades: A and B with similarity about 47%, but clade B was represented by only one isolate. The clade A was divided in two subclades A.I NS .II with similarity 53,2% and then into four groups: A.Ia, A.Ib, A.Ic and A.Id. The comparative analysis did not distinguish clearly toxigenic and nontoxigenic isolates as well as invasive and noninvasive isolates.
Whole cell protein profile analysis of C. diphtheria exhibits good concordance with other genotyping methods but this method is not able to distinguish clearly invasive from non-invasive isolates.</abstract><cop>Poland</cop><pmid>30376615</pmid><tpages>6</tpages></addata></record> |
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| subjects | Bacterial Proteins Bacterial Typing Techniques Corynebacterium diphtheriae - classification Corynebacterium diphtheriae - pathogenicity Diphtheria - genetics Diphtheria - metabolism Electrophoresis, Polyacrylamide Gel Humans |
| title | Whole cell protein profiling characterization of Corynebacterium diphtheriae clinical isolates collected from various infections |
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