Methylation Analysis and Validation of Whole Genome DNA in Active Tuberculosis
To screen and identify the gene of DNA methylation in patients with active tuberculosis. ① This study enrolled 9 cases of active tuberculosis patients (including 3 newly diagnosed tuberculosis patients and 6 cases of retreatment of active tuberculosis patients), 3 cases of latent tuberculosis patien...
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| Veröffentlicht in: | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2018-09, Vol.49 (5), p.731-736 |
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| container_title | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban |
| container_volume | 49 |
| creator | Chen, Hao Zhang, Jing-Ya Hu, Xue-Jiao Lu, Xiao-Jun Wang, Jun Zhou, Yan-Hong Ying, Bin-Wu Zhou, Jing |
| description | To screen and identify the gene of DNA methylation in patients with active tuberculosis.
① This study enrolled 9 cases of active tuberculosis patients (including 3 newly diagnosed tuberculosis patients and 6 cases of retreatment of active tuberculosis patients), 3 cases of latent tuberculosis patients and 3 cases of healthy controls. Genome DNA was extracted from Peripheral Blood Mononuclear Cell and following bisulfite conversion treatment. After hybridization with the Illumina HD 450K Infinium Mehtylation BeadChip, the results were compared between patients group and control group, GO and Pathway analysis were performed to evaluate the function of differentially expressed genes; ② We further enrolled 60 cases of active tuberculosis patients and 60 cases of health controls (their age and gender were matched). By using pyrosequencing method to detect the methylation levels of candidate genes
screened by gene chip.
① Compared with healthy controls, we found that most of them were showed demethylation status. G |
| format | Article |
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① This study enrolled 9 cases of active tuberculosis patients (including 3 newly diagnosed tuberculosis patients and 6 cases of retreatment of active tuberculosis patients), 3 cases of latent tuberculosis patients and 3 cases of healthy controls. Genome DNA was extracted from Peripheral Blood Mononuclear Cell and following bisulfite conversion treatment. After hybridization with the Illumina HD 450K Infinium Mehtylation BeadChip, the results were compared between patients group and control group, GO and Pathway analysis were performed to evaluate the function of differentially expressed genes; ② We further enrolled 60 cases of active tuberculosis patients and 60 cases of health controls (their age and gender were matched). By using pyrosequencing method to detect the methylation levels of candidate genes
screened by gene chip.
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① This study enrolled 9 cases of active tuberculosis patients (including 3 newly diagnosed tuberculosis patients and 6 cases of retreatment of active tuberculosis patients), 3 cases of latent tuberculosis patients and 3 cases of healthy controls. Genome DNA was extracted from Peripheral Blood Mononuclear Cell and following bisulfite conversion treatment. After hybridization with the Illumina HD 450K Infinium Mehtylation BeadChip, the results were compared between patients group and control group, GO and Pathway analysis were performed to evaluate the function of differentially expressed genes; ② We further enrolled 60 cases of active tuberculosis patients and 60 cases of health controls (their age and gender were matched). By using pyrosequencing method to detect the methylation levels of candidate genes
screened by gene chip.
① Compared with healthy controls, we found that most of them were showed demethylation status. G</description><subject>Case-Control Studies</subject><subject>CpG Islands</subject><subject>DNA Methylation</subject><subject>Humans</subject><subject>Leukocytes, Mononuclear</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Toll-Like Receptor 1 - genetics</subject><subject>Toll-Like Receptor 2 - genetics</subject><subject>Toll-Like Receptor 4 - genetics</subject><subject>Tuberculosis - genetics</subject><issn>1672-173X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kD1rwzAURTW0NCHNXygauxgkP0uWRpO2aSFNl_RjM7L8TASy5Vp2If--hiTThcs5d7g3ZMllniY8h58FWcfoKsY4Z6BVfkcWwCBXAGJJ9u84Hk_ejC50tOiMP0UXqelq-mW8q899aOj3MXikW-xCi_RpX1A343Z0f0gPU4WDnXyYzXty2xgfcX3JFfl8eT5sXpPdx_ZtU-ySnqdyTCoDGZO11lZao4BbbZAZVWvkukGJyBAzLazMVAqishwEKgm1RdEonaWwIo_n3X4IvxPGsWxdtOi96TBMsUx5mkuRZ5LN6MMFnaoW67IfXGuGU3n9AP4Bc3JX3w</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Chen, Hao</creator><creator>Zhang, Jing-Ya</creator><creator>Hu, Xue-Jiao</creator><creator>Lu, Xiao-Jun</creator><creator>Wang, Jun</creator><creator>Zhou, Yan-Hong</creator><creator>Ying, Bin-Wu</creator><creator>Zhou, Jing</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201809</creationdate><title>Methylation Analysis and Validation of Whole Genome DNA in Active Tuberculosis</title><author>Chen, Hao ; Zhang, Jing-Ya ; Hu, Xue-Jiao ; Lu, Xiao-Jun ; Wang, Jun ; Zhou, Yan-Hong ; Ying, Bin-Wu ; Zhou, Jing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-ba3406d99c6ca831c9ae0a8d9e19fe6ee0ee495c648235bc135e863dce5f89423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2018</creationdate><topic>Case-Control Studies</topic><topic>CpG Islands</topic><topic>DNA Methylation</topic><topic>Humans</topic><topic>Leukocytes, Mononuclear</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Toll-Like Receptor 1 - genetics</topic><topic>Toll-Like Receptor 2 - genetics</topic><topic>Toll-Like Receptor 4 - genetics</topic><topic>Tuberculosis - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Chen, Hao</creatorcontrib><creatorcontrib>Zhang, Jing-Ya</creatorcontrib><creatorcontrib>Hu, Xue-Jiao</creatorcontrib><creatorcontrib>Lu, Xiao-Jun</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><creatorcontrib>Zhou, Yan-Hong</creatorcontrib><creatorcontrib>Ying, Bin-Wu</creatorcontrib><creatorcontrib>Zhou, Jing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Hao</au><au>Zhang, Jing-Ya</au><au>Hu, Xue-Jiao</au><au>Lu, Xiao-Jun</au><au>Wang, Jun</au><au>Zhou, Yan-Hong</au><au>Ying, Bin-Wu</au><au>Zhou, Jing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methylation Analysis and Validation of Whole Genome DNA in Active Tuberculosis</atitle><jtitle>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</jtitle><addtitle>Sichuan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2018-09</date><risdate>2018</risdate><volume>49</volume><issue>5</issue><spage>731</spage><epage>736</epage><pages>731-736</pages><issn>1672-173X</issn><abstract>To screen and identify the gene of DNA methylation in patients with active tuberculosis.
① This study enrolled 9 cases of active tuberculosis patients (including 3 newly diagnosed tuberculosis patients and 6 cases of retreatment of active tuberculosis patients), 3 cases of latent tuberculosis patients and 3 cases of healthy controls. Genome DNA was extracted from Peripheral Blood Mononuclear Cell and following bisulfite conversion treatment. After hybridization with the Illumina HD 450K Infinium Mehtylation BeadChip, the results were compared between patients group and control group, GO and Pathway analysis were performed to evaluate the function of differentially expressed genes; ② We further enrolled 60 cases of active tuberculosis patients and 60 cases of health controls (their age and gender were matched). By using pyrosequencing method to detect the methylation levels of candidate genes
screened by gene chip.
① Compared with healthy controls, we found that most of them were showed demethylation status. G</abstract><cop>China</cop><pmid>30378335</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2018-09, Vol.49 (5), p.731-736 |
| issn | 1672-173X |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Case-Control Studies CpG Islands DNA Methylation Humans Leukocytes, Mononuclear Oligonucleotide Array Sequence Analysis Toll-Like Receptor 1 - genetics Toll-Like Receptor 2 - genetics Toll-Like Receptor 4 - genetics Tuberculosis - genetics |
| title | Methylation Analysis and Validation of Whole Genome DNA in Active Tuberculosis |
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