Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis
To establish Gli1-CreER ; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis. Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreER mice (Gli1 mice) were obtained, with Gli1-CreER ; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced wi...
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| Veröffentlicht in: | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2018-07, Vol.49 (4), p.560-565 |
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| container_title | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban |
| container_volume | 49 |
| creator | Sun, Jing Li, Can Ma, Yan Guo, Li-Na Tian, Mao-Cen Liu, Qin Liu, Wei-Hua Wu, Jiang Deng, Feng-Mei Liu, Yi-Lun |
| description | To establish Gli1-CreER
; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis.
Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreER
mice (Gli1 mice) were obtained, with Gli1-CreER
; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced with CCl
. Their liver samples were taken. The formalin-fixed and paraffin-embedded samples were prepared for HE staining and Masson staining. The expression of tdTomato was observed under immunofluorescent microscope.
An ideal number of Gli1-CreER
; tdTomato genetic lineage-tracing mice were harvested. The differences in fertility between the parental and the offspring mice were not significant (
>0.05). Pseudolobular formation occurred in the CCl
-induced hepatic fibrosis model mice. Enhanced red fluoresce was observed in the model mice.
Gli1-CreER
; tdTomato genetic lineage-tracing mice can be used to monitor the cell source of fibrous tissues, its transition as well as the underlying mechanism of pathogenesis of |
| format | Article |
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; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis.
Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreER
mice (Gli1 mice) were obtained, with Gli1-CreER
; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced with CCl
. Their liver samples were taken. The formalin-fixed and paraffin-embedded samples were prepared for HE staining and Masson staining. The expression of tdTomato was observed under immunofluorescent microscope.
An ideal number of Gli1-CreER
; tdTomato genetic lineage-tracing mice were harvested. The differences in fertility between the parental and the offspring mice were not significant (
>0.05). Pseudolobular formation occurred in the CCl
-induced hepatic fibrosis model mice. Enhanced red fluoresce was observed in the model mice.
Gli1-CreER
; tdTomato genetic lineage-tracing mice can be used to monitor the cell source of fibrous tissues, its transition as well as the underlying mechanism of pathogenesis of</description><identifier>ISSN: 1672-173X</identifier><identifier>PMID: 30378310</identifier><language>chi</language><publisher>China</publisher><subject>Animals ; Liver Cirrhosis - genetics ; Liver Cirrhosis - pathology ; Mice ; Mice, Transgenic ; Zinc Finger Protein GLI1 - genetics</subject><ispartof>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2018-07, Vol.49 (4), p.560-565</ispartof><rights>Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30378310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Li, Can</creatorcontrib><creatorcontrib>Ma, Yan</creatorcontrib><creatorcontrib>Guo, Li-Na</creatorcontrib><creatorcontrib>Tian, Mao-Cen</creatorcontrib><creatorcontrib>Liu, Qin</creatorcontrib><creatorcontrib>Liu, Wei-Hua</creatorcontrib><creatorcontrib>Wu, Jiang</creatorcontrib><creatorcontrib>Deng, Feng-Mei</creatorcontrib><creatorcontrib>Liu, Yi-Lun</creatorcontrib><title>Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis</title><title>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</title><addtitle>Sichuan Da Xue Xue Bao Yi Xue Ban</addtitle><description>To establish Gli1-CreER
; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis.
Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreER
mice (Gli1 mice) were obtained, with Gli1-CreER
; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced with CCl
. Their liver samples were taken. The formalin-fixed and paraffin-embedded samples were prepared for HE staining and Masson staining. The expression of tdTomato was observed under immunofluorescent microscope.
An ideal number of Gli1-CreER
; tdTomato genetic lineage-tracing mice were harvested. The differences in fertility between the parental and the offspring mice were not significant (
>0.05). Pseudolobular formation occurred in the CCl
-induced hepatic fibrosis model mice. Enhanced red fluoresce was observed in the model mice.
Gli1-CreER
; tdTomato genetic lineage-tracing mice can be used to monitor the cell source of fibrous tissues, its transition as well as the underlying mechanism of pathogenesis of</description><subject>Animals</subject><subject>Liver Cirrhosis - genetics</subject><subject>Liver Cirrhosis - pathology</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Zinc Finger Protein GLI1 - genetics</subject><issn>1672-173X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kDtrwzAUhTW0NCHNXygauxj0SCx5LCGPQkqGttDN6HHtCmzJ9ZWH_vsmNJkOB77vDOeOzHmpRMGV_JqRJWKwjHHOZKXVA5lJJpWWnM1J3GI2tgv4HWJL911IvSkMYnLBZPD0FF1qIQI9pD51qaWc7s81B0ePIYJpocijcRf5LTigTRrpe558AKQp0gMM5sLugh0TBnwk943pEJbXXJDP3fZjcyiOp_3r5uVYDFyUudBOMWYapYxeV6umWkunmQNg3q-sUMID196wBqwQpdeVrJS2ChrJRMkks3JBnv93hzH9TIC57gM66DoTIU1YCy5UuVai0mf06YpOtgdfD2Pozfhb3z6Sf-tLY0E</recordid><startdate>201807</startdate><enddate>201807</enddate><creator>Sun, Jing</creator><creator>Li, Can</creator><creator>Ma, Yan</creator><creator>Guo, Li-Na</creator><creator>Tian, Mao-Cen</creator><creator>Liu, Qin</creator><creator>Liu, Wei-Hua</creator><creator>Wu, Jiang</creator><creator>Deng, Feng-Mei</creator><creator>Liu, Yi-Lun</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201807</creationdate><title>Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis</title><author>Sun, Jing ; Li, Can ; Ma, Yan ; Guo, Li-Na ; Tian, Mao-Cen ; Liu, Qin ; Liu, Wei-Hua ; Wu, Jiang ; Deng, Feng-Mei ; Liu, Yi-Lun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-8c700af77a8594f953c80cee0dd4b272de18da0feb226d893978b7ef3026030b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Liver Cirrhosis - genetics</topic><topic>Liver Cirrhosis - pathology</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Zinc Finger Protein GLI1 - genetics</topic><toplevel>online_resources</toplevel><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Li, Can</creatorcontrib><creatorcontrib>Ma, Yan</creatorcontrib><creatorcontrib>Guo, Li-Na</creatorcontrib><creatorcontrib>Tian, Mao-Cen</creatorcontrib><creatorcontrib>Liu, Qin</creatorcontrib><creatorcontrib>Liu, Wei-Hua</creatorcontrib><creatorcontrib>Wu, Jiang</creatorcontrib><creatorcontrib>Deng, Feng-Mei</creatorcontrib><creatorcontrib>Liu, Yi-Lun</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Jing</au><au>Li, Can</au><au>Ma, Yan</au><au>Guo, Li-Na</au><au>Tian, Mao-Cen</au><au>Liu, Qin</au><au>Liu, Wei-Hua</au><au>Wu, Jiang</au><au>Deng, Feng-Mei</au><au>Liu, Yi-Lun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis</atitle><jtitle>Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban</jtitle><addtitle>Sichuan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2018-07</date><risdate>2018</risdate><volume>49</volume><issue>4</issue><spage>560</spage><epage>565</epage><pages>560-565</pages><issn>1672-173X</issn><abstract>To establish Gli1-CreER
; tdTomato genetic lineage-tracing mice for studies on hepatic fibrosis.
Offspring of ROSA26 td Tomato (tdTomato) mice and Gli1-CreER
mice (Gli1 mice) were obtained, with Gli1-CreER
; tdTomato genotype being identified by PCR. The mice model of hepatic fibrosis was induced with CCl
. Their liver samples were taken. The formalin-fixed and paraffin-embedded samples were prepared for HE staining and Masson staining. The expression of tdTomato was observed under immunofluorescent microscope.
An ideal number of Gli1-CreER
; tdTomato genetic lineage-tracing mice were harvested. The differences in fertility between the parental and the offspring mice were not significant (
>0.05). Pseudolobular formation occurred in the CCl
-induced hepatic fibrosis model mice. Enhanced red fluoresce was observed in the model mice.
Gli1-CreER
; tdTomato genetic lineage-tracing mice can be used to monitor the cell source of fibrous tissues, its transition as well as the underlying mechanism of pathogenesis of</abstract><cop>China</cop><pmid>30378310</pmid><tpages>6</tpages></addata></record> |
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| ispartof | Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban, 2018-07, Vol.49 (4), p.560-565 |
| issn | 1672-173X |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Liver Cirrhosis - genetics Liver Cirrhosis - pathology Mice Mice, Transgenic Zinc Finger Protein GLI1 - genetics |
| title | Establishing Glioma-associated Oncogene Homolog 1 Genetic Lineage-tracing Mice for Studies on Hepatic Fibrosis |
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