Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent
Oxidative stress due to the overproduction of reactive oxygen species plays an important role in the pathogenesis of various diseases. In the present study, we comprehensively evaluated the antioxidant activities of 147 oral formulations of Japanese traditional herbal medicines (Kampo medicines), re...
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| Veröffentlicht in: | Journal of natural medicines 2019-01, Vol.73 (1), p.163-172 |
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| creator | Sato, Naoko Li, Wei Takemoto, Hiroaki Takeuchi, Mio Nakamura, Ai Tokura, Emi Akahane, Chie Ueno, Kanako Komatsu, Kana Kuriyama, Noriko Onoda, Toshihisa Higai, Koji Koike, Kazuo |
| description | Oxidative stress due to the overproduction of reactive oxygen species plays an important role in the pathogenesis of various diseases. In the present study, we comprehensively evaluated the antioxidant activities of 147 oral formulations of Japanese traditional herbal medicines (Kampo medicines), representing the entire panel of oral Kampo medicines listed in the Japanese National Health Insurance Drug List, using in vitro radical scavenging assays, including the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay, the superoxide anion scavenging activity assay, and the oxygen radical absorption capacity assay. Three of the formulations tested, namely, Tsudosan, Daisaikoto, and Masiningan, showed the most potent in vitro antioxidant activities and were selected for further investigation of their intracellular and in vivo antioxidant effects. The results of the 2′,7′-dichlorodihydrofluorescin diacetate assay demonstrated that all three Kampo medicines significantly inhibited hydrogen peroxide-induced oxidative stress in human hepatocellular liver carcinoma HepG2 cells. In addition, Tsudosan significantly increased the serum biological antioxidant potential values when orally administrated to mice, indicating that it also had in vivo antioxidant activity. The potent antioxidant activity of Tsudosan may be one of the mechanisms closely correlated to its clinical usage against blood stasis. |
| doi_str_mv | 10.1007/s11418-018-1259-x |
| format | Article |
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In the present study, we comprehensively evaluated the antioxidant activities of 147 oral formulations of Japanese traditional herbal medicines (Kampo medicines), representing the entire panel of oral Kampo medicines listed in the Japanese National Health Insurance Drug List, using in vitro radical scavenging assays, including the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay, the superoxide anion scavenging activity assay, and the oxygen radical absorption capacity assay. Three of the formulations tested, namely, Tsudosan, Daisaikoto, and Masiningan, showed the most potent in vitro antioxidant activities and were selected for further investigation of their intracellular and in vivo antioxidant effects. The results of the 2′,7′-dichlorodihydrofluorescin diacetate assay demonstrated that all three Kampo medicines significantly inhibited hydrogen peroxide-induced oxidative stress in human hepatocellular liver carcinoma HepG2 cells. In addition, Tsudosan significantly increased the serum biological antioxidant potential values when orally administrated to mice, indicating that it also had in vivo antioxidant activity. The potent antioxidant activity of Tsudosan may be one of the mechanisms closely correlated to its clinical usage against blood stasis.</description><identifier>ISSN: 1340-3443</identifier><identifier>EISSN: 1861-0293</identifier><identifier>DOI: 10.1007/s11418-018-1259-x</identifier><identifier>PMID: 30374696</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Animals ; Antioxidants ; Antioxidants - pharmacology ; Antioxidants - therapeutic use ; Biomedical and Life Sciences ; Biomedicine ; Complementary & Alternative Medicine ; Herbal medicine ; Humans ; Japan ; Liver cancer ; Medicinal Chemistry ; Medicine, Kampo - methods ; Original Paper ; Oxidation ; Oxidative stress ; Pharmacology/Toxicology ; Pharmacy ; Phytochemicals ; Plant Sciences ; Plants, Medicinal - drug effects ; Reactive Oxygen Species</subject><ispartof>Journal of natural medicines, 2019-01, Vol.73 (1), p.163-172</ispartof><rights>The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2018</rights><rights>The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2018.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c489t-fe68d4ed89c6b69a3c6887157459cd52f9f7a3d028b72f3d70c88e79593d15bf3</citedby><cites>FETCH-LOGICAL-c489t-fe68d4ed89c6b69a3c6887157459cd52f9f7a3d028b72f3d70c88e79593d15bf3</cites><orcidid>0000-0003-4143-8597</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11418-018-1259-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11418-018-1259-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30374696$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sato, Naoko</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Takemoto, Hiroaki</creatorcontrib><creatorcontrib>Takeuchi, Mio</creatorcontrib><creatorcontrib>Nakamura, Ai</creatorcontrib><creatorcontrib>Tokura, Emi</creatorcontrib><creatorcontrib>Akahane, Chie</creatorcontrib><creatorcontrib>Ueno, Kanako</creatorcontrib><creatorcontrib>Komatsu, Kana</creatorcontrib><creatorcontrib>Kuriyama, Noriko</creatorcontrib><creatorcontrib>Onoda, Toshihisa</creatorcontrib><creatorcontrib>Higai, Koji</creatorcontrib><creatorcontrib>Koike, Kazuo</creatorcontrib><title>Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent</title><title>Journal of natural medicines</title><addtitle>J Nat Med</addtitle><addtitle>J Nat Med</addtitle><description>Oxidative stress due to the overproduction of reactive oxygen species plays an important role in the pathogenesis of various diseases. In the present study, we comprehensively evaluated the antioxidant activities of 147 oral formulations of Japanese traditional herbal medicines (Kampo medicines), representing the entire panel of oral Kampo medicines listed in the Japanese National Health Insurance Drug List, using in vitro radical scavenging assays, including the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay, the superoxide anion scavenging activity assay, and the oxygen radical absorption capacity assay. Three of the formulations tested, namely, Tsudosan, Daisaikoto, and Masiningan, showed the most potent in vitro antioxidant activities and were selected for further investigation of their intracellular and in vivo antioxidant effects. The results of the 2′,7′-dichlorodihydrofluorescin diacetate assay demonstrated that all three Kampo medicines significantly inhibited hydrogen peroxide-induced oxidative stress in human hepatocellular liver carcinoma HepG2 cells. In addition, Tsudosan significantly increased the serum biological antioxidant potential values when orally administrated to mice, indicating that it also had in vivo antioxidant activity. The potent antioxidant activity of Tsudosan may be one of the mechanisms closely correlated to its clinical usage against blood stasis.</description><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - pharmacology</subject><subject>Antioxidants - therapeutic use</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Complementary & Alternative Medicine</subject><subject>Herbal medicine</subject><subject>Humans</subject><subject>Japan</subject><subject>Liver cancer</subject><subject>Medicinal Chemistry</subject><subject>Medicine, Kampo - methods</subject><subject>Original Paper</subject><subject>Oxidation</subject><subject>Oxidative stress</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Phytochemicals</subject><subject>Plant Sciences</subject><subject>Plants, Medicinal - drug effects</subject><subject>Reactive Oxygen Species</subject><issn>1340-3443</issn><issn>1861-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1u1TAQhSMEoj_wAGyQJTZsAv5LbC_RVYFCJTZlbfna4-IqiYOdVJcn4XWZq5QikFhYYx1_c8b2aZoXjL5hlKq3lTHJdEtxMd6Z9vCoOWW6Zy3lRjzGvZC0FVKKk-as1ltKJReCPW1OBBVK9qY_bX7u8jgX-AZTTXdA4M4Nq1tSnkiOxE24O6SAlUCM4Jd6lD-52U1QgXx245zJCCH5hAIZIJAlkxQAG2PyD0bXdQ25uonEXMZ12HRXiSNzXhD-a5K7QeVZ8yS6ocLz-3refH1_cb372F59-XC5e3fVeqnN0kbodZAQtPH9vjdO-F5rxTolO-NDx6OJyolAud4rHkVQ1GsNynRGBNbtozhvXm--c8nfV6iLHVP1MAz4wrxWyxlXnHKpGaKv_kFv81omvB1SfYdRCMORYhvlS661QLRzSaMrPyyj9pia3VKzmJo9pmYP2PPy3nnd428-dPyOCQG-ARWPphsof0b_3_UXD0GlyQ</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>Sato, Naoko</creator><creator>Li, Wei</creator><creator>Takemoto, Hiroaki</creator><creator>Takeuchi, Mio</creator><creator>Nakamura, Ai</creator><creator>Tokura, Emi</creator><creator>Akahane, Chie</creator><creator>Ueno, Kanako</creator><creator>Komatsu, Kana</creator><creator>Kuriyama, Noriko</creator><creator>Onoda, Toshihisa</creator><creator>Higai, Koji</creator><creator>Koike, Kazuo</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4143-8597</orcidid></search><sort><creationdate>20190101</creationdate><title>Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent</title><author>Sato, Naoko ; Li, Wei ; Takemoto, Hiroaki ; Takeuchi, Mio ; Nakamura, Ai ; Tokura, Emi ; Akahane, Chie ; Ueno, Kanako ; Komatsu, Kana ; Kuriyama, Noriko ; Onoda, Toshihisa ; Higai, Koji ; Koike, Kazuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c489t-fe68d4ed89c6b69a3c6887157459cd52f9f7a3d028b72f3d70c88e79593d15bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - pharmacology</topic><topic>Antioxidants - therapeutic use</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Complementary & Alternative Medicine</topic><topic>Herbal medicine</topic><topic>Humans</topic><topic>Japan</topic><topic>Liver cancer</topic><topic>Medicinal Chemistry</topic><topic>Medicine, Kampo - methods</topic><topic>Original Paper</topic><topic>Oxidation</topic><topic>Oxidative stress</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Phytochemicals</topic><topic>Plant Sciences</topic><topic>Plants, Medicinal - drug effects</topic><topic>Reactive Oxygen Species</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sato, Naoko</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Takemoto, Hiroaki</creatorcontrib><creatorcontrib>Takeuchi, Mio</creatorcontrib><creatorcontrib>Nakamura, Ai</creatorcontrib><creatorcontrib>Tokura, Emi</creatorcontrib><creatorcontrib>Akahane, Chie</creatorcontrib><creatorcontrib>Ueno, Kanako</creatorcontrib><creatorcontrib>Komatsu, Kana</creatorcontrib><creatorcontrib>Kuriyama, Noriko</creatorcontrib><creatorcontrib>Onoda, Toshihisa</creatorcontrib><creatorcontrib>Higai, Koji</creatorcontrib><creatorcontrib>Koike, Kazuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sato, Naoko</au><au>Li, Wei</au><au>Takemoto, Hiroaki</au><au>Takeuchi, Mio</au><au>Nakamura, Ai</au><au>Tokura, Emi</au><au>Akahane, Chie</au><au>Ueno, Kanako</au><au>Komatsu, Kana</au><au>Kuriyama, Noriko</au><au>Onoda, Toshihisa</au><au>Higai, Koji</au><au>Koike, Kazuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent</atitle><jtitle>Journal of natural medicines</jtitle><stitle>J Nat Med</stitle><addtitle>J Nat Med</addtitle><date>2019-01-01</date><risdate>2019</risdate><volume>73</volume><issue>1</issue><spage>163</spage><epage>172</epage><pages>163-172</pages><issn>1340-3443</issn><eissn>1861-0293</eissn><abstract>Oxidative stress due to the overproduction of reactive oxygen species plays an important role in the pathogenesis of various diseases. In the present study, we comprehensively evaluated the antioxidant activities of 147 oral formulations of Japanese traditional herbal medicines (Kampo medicines), representing the entire panel of oral Kampo medicines listed in the Japanese National Health Insurance Drug List, using in vitro radical scavenging assays, including the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity assay, the superoxide anion scavenging activity assay, and the oxygen radical absorption capacity assay. Three of the formulations tested, namely, Tsudosan, Daisaikoto, and Masiningan, showed the most potent in vitro antioxidant activities and were selected for further investigation of their intracellular and in vivo antioxidant effects. The results of the 2′,7′-dichlorodihydrofluorescin diacetate assay demonstrated that all three Kampo medicines significantly inhibited hydrogen peroxide-induced oxidative stress in human hepatocellular liver carcinoma HepG2 cells. 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| subjects | Animals Antioxidants Antioxidants - pharmacology Antioxidants - therapeutic use Biomedical and Life Sciences Biomedicine Complementary & Alternative Medicine Herbal medicine Humans Japan Liver cancer Medicinal Chemistry Medicine, Kampo - methods Original Paper Oxidation Oxidative stress Pharmacology/Toxicology Pharmacy Phytochemicals Plant Sciences Plants, Medicinal - drug effects Reactive Oxygen Species |
| title | Comprehensive evaluation of antioxidant effects of Japanese Kampo medicines led to identification of Tsudosan formulation as a potent antioxidant agent |
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