Molecular characterization and phylogenetic analysis of Taenia multiceps from China
Taenia multiceps , one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of T.multiceps is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of T.multiceps and coenur...
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| creator | Tan, L. Wang, A. B. Zheng, S. Q. Zhang, X. L. Huang, C. J. Liu, W. |
| description | Taenia multiceps
, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of
T.multiceps
is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of
T.multiceps
and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of
T.multiceps
were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(p
cox
1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from
T.multiceps
and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between
T.multiceps
and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for p
cox
1, 12S rRNA and ITS, respectively, among
T.multiceps
and coenurus isolates obtained in this study. In Sichuan province, sequence variations for
Co-enurus cerebralis
isolated from Yaan city were 0–1.4% for p
cox
1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of
T.multiceps
isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for p
cox
1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on p
cox
1 sequences indicated that all cerebral and non-cerebral metacestodes belong to
T.multiceps
. These results provide reference values for future molecular epidemiological and biological study on
T.multiceps
in dogs and intermediate hosts. |
| doi_str_mv | 10.1515/ap-2018-0085 |
| format | Article |
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, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of
T.multiceps
is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of
T.multiceps
and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of
T.multiceps
were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(p
cox
1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from
T.multiceps
and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between
T.multiceps
and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for p
cox
1, 12S rRNA and ITS, respectively, among
T.multiceps
and coenurus isolates obtained in this study. In Sichuan province, sequence variations for
Co-enurus cerebralis
isolated from Yaan city were 0–1.4% for p
cox
1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of
T.multiceps
isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for p
cox
1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on p
cox
1 sequences indicated that all cerebral and non-cerebral metacestodes belong to
T.multiceps
. These results provide reference values for future molecular epidemiological and biological study on
T.multiceps
in dogs and intermediate hosts.</description><identifier>ISSN: 1230-2821</identifier><identifier>EISSN: 1896-1851</identifier><identifier>DOI: 10.1515/ap-2018-0085</identifier><identifier>PMID: 30367774</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Systematics/Taxonomy/Biogeography ; Animals ; Biomedical and Life Sciences ; Biomedicine ; China ; Cyclooxygenase 1 - genetics ; Cytochrome ; Cytochromes ; Deoxyribonucleic acid ; DNA ; DNA, Helminth - genetics ; DNA, Helminth - isolation & purification ; DNA, Intergenic - genetics ; DNA, Mitochondrial - genetics ; Dogs ; Ecology ; Economic impact ; Epidemiology ; Gene sequencing ; Genetic Markers ; Genetic Variation ; Goat Diseases - parasitology ; Goats ; internal transcribed spacer (ITS) ; Livestock ; Medical Microbiology ; Microbiology ; Mitochondrial DNA ; mitochondrial DNA (mtDNA) ; Nucleotide sequence ; Nucleotides ; Original Paper ; Parasites ; Parasitology ; phylogenetic analysis ; Phylogenetics ; Phylogeny ; Polymerase Chain Reaction - veterinary ; Ribosomal DNA ; RNA, Ribosomal - genetics ; rRNA 12S ; Sequence Alignment - veterinary ; Small intestine ; Spacer ; Taenia - classification ; Taenia - genetics ; Taenia - growth & development ; Taenia multiceps ; Taeniasis - parasitology ; Taeniasis - veterinary ; Variation</subject><ispartof>Acta parasitologica, 2018-12, Vol.63 (4), p.721-727</ispartof><rights>Witold Stefański Institute of Parasitology, Polish Academy of Sciences 2018</rights><rights>2018 W. Stefański Institute of Parasitology, PAS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-706c28a1ed853bfbfa6794d530207409b82046941492167d2d1a39847a15d5633</citedby><cites>FETCH-LOGICAL-c408t-706c28a1ed853bfbfa6794d530207409b82046941492167d2d1a39847a15d5633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1515/ap-2018-0085$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1515/ap-2018-0085$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30367774$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tan, L.</creatorcontrib><creatorcontrib>Wang, A. B.</creatorcontrib><creatorcontrib>Zheng, S. Q.</creatorcontrib><creatorcontrib>Zhang, X. L.</creatorcontrib><creatorcontrib>Huang, C. J.</creatorcontrib><creatorcontrib>Liu, W.</creatorcontrib><title>Molecular characterization and phylogenetic analysis of Taenia multiceps from China</title><title>Acta parasitologica</title><addtitle>Acta Parasit</addtitle><addtitle>Acta Parasitol</addtitle><description>Taenia multiceps
, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of
T.multiceps
is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of
T.multiceps
and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of
T.multiceps
were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(p
cox
1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from
T.multiceps
and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between
T.multiceps
and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for p
cox
1, 12S rRNA and ITS, respectively, among
T.multiceps
and coenurus isolates obtained in this study. In Sichuan province, sequence variations for
Co-enurus cerebralis
isolated from Yaan city were 0–1.4% for p
cox
1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of
T.multiceps
isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for p
cox
1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on p
cox
1 sequences indicated that all cerebral and non-cerebral metacestodes belong to
T.multiceps
. These results provide reference values for future molecular epidemiological and biological study on
T.multiceps
in dogs and intermediate hosts.</description><subject>Animal Systematics/Taxonomy/Biogeography</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>China</subject><subject>Cyclooxygenase 1 - genetics</subject><subject>Cytochrome</subject><subject>Cytochromes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Helminth - genetics</subject><subject>DNA, Helminth - isolation & purification</subject><subject>DNA, Intergenic - genetics</subject><subject>DNA, Mitochondrial - genetics</subject><subject>Dogs</subject><subject>Ecology</subject><subject>Economic impact</subject><subject>Epidemiology</subject><subject>Gene sequencing</subject><subject>Genetic Markers</subject><subject>Genetic Variation</subject><subject>Goat Diseases - parasitology</subject><subject>Goats</subject><subject>internal transcribed spacer (ITS)</subject><subject>Livestock</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Mitochondrial DNA</subject><subject>mitochondrial DNA (mtDNA)</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Parasites</subject><subject>Parasitology</subject><subject>phylogenetic analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Ribosomal DNA</subject><subject>RNA, Ribosomal - genetics</subject><subject>rRNA 12S</subject><subject>Sequence Alignment - veterinary</subject><subject>Small intestine</subject><subject>Spacer</subject><subject>Taenia - classification</subject><subject>Taenia - genetics</subject><subject>Taenia - growth & development</subject><subject>Taenia multiceps</subject><subject>Taeniasis - parasitology</subject><subject>Taeniasis - veterinary</subject><subject>Variation</subject><issn>1230-2821</issn><issn>1896-1851</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkMFr2zAUh8VoWdJ0t52LoZcd6k5PkiV5txK2ttDSw9KzebHlxEG2PMlmpH_9ZJJ1UAo76SF97_eePkI-A72GDLKv2KeMgk4p1dkHMgedyxR0BiexZpymTDOYkbMQdpQKqbX-SGaccqmUEnPy89FZU44WfVJu0WM5GN-84NC4LsGuSvrt3rqN6czQlPEC7T40IXF1skLTNZi0o40vpg9J7V2bLLdNh-fktEYbzKfjuSDPP76vlnfpw9Pt_fLmIS0F1UOqqCyZRjCVzvi6XtcoVS6qjFNGlaD5WrO4cC5A5AykqlgFyHMtFEJWZZLzBflyyO29-zWaMBRtE0pjLXbGjaFgwGQOSvMJvXyD7tzo43cixaIVACEhUlcHqvQuBG_qovdNi35fAC0m2QX2xSS7mGRH_OIYOq5bU73Cf-1G4NsB-I02eq3Mxo_7WPwb_l6u5EKxaZn00Bxibrf5Xxf_A_7Pmdo</recordid><startdate>20181219</startdate><enddate>20181219</enddate><creator>Tan, L.</creator><creator>Wang, A. B.</creator><creator>Zheng, S. Q.</creator><creator>Zhang, X. L.</creator><creator>Huang, C. J.</creator><creator>Liu, W.</creator><general>Springer International Publishing</general><general>De Gruyter</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20181219</creationdate><title>Molecular characterization and phylogenetic analysis of Taenia multiceps from China</title><author>Tan, L. ; Wang, A. B. ; Zheng, S. Q. ; Zhang, X. L. ; Huang, C. J. ; Liu, W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-706c28a1ed853bfbfa6794d530207409b82046941492167d2d1a39847a15d5633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Systematics/Taxonomy/Biogeography</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>China</topic><topic>Cyclooxygenase 1 - genetics</topic><topic>Cytochrome</topic><topic>Cytochromes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Helminth - genetics</topic><topic>DNA, Helminth - isolation & purification</topic><topic>DNA, Intergenic - genetics</topic><topic>DNA, Mitochondrial - genetics</topic><topic>Dogs</topic><topic>Ecology</topic><topic>Economic impact</topic><topic>Epidemiology</topic><topic>Gene sequencing</topic><topic>Genetic Markers</topic><topic>Genetic Variation</topic><topic>Goat Diseases - parasitology</topic><topic>Goats</topic><topic>internal transcribed spacer (ITS)</topic><topic>Livestock</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Mitochondrial DNA</topic><topic>mitochondrial DNA (mtDNA)</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Original Paper</topic><topic>Parasites</topic><topic>Parasitology</topic><topic>phylogenetic analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Ribosomal DNA</topic><topic>RNA, Ribosomal - genetics</topic><topic>rRNA 12S</topic><topic>Sequence Alignment - veterinary</topic><topic>Small intestine</topic><topic>Spacer</topic><topic>Taenia - classification</topic><topic>Taenia - genetics</topic><topic>Taenia - growth & development</topic><topic>Taenia multiceps</topic><topic>Taeniasis - parasitology</topic><topic>Taeniasis - veterinary</topic><topic>Variation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tan, L.</creatorcontrib><creatorcontrib>Wang, A. B.</creatorcontrib><creatorcontrib>Zheng, S. Q.</creatorcontrib><creatorcontrib>Zhang, X. L.</creatorcontrib><creatorcontrib>Huang, C. J.</creatorcontrib><creatorcontrib>Liu, W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta parasitologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tan, L.</au><au>Wang, A. B.</au><au>Zheng, S. Q.</au><au>Zhang, X. L.</au><au>Huang, C. J.</au><au>Liu, W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular characterization and phylogenetic analysis of Taenia multiceps from China</atitle><jtitle>Acta parasitologica</jtitle><stitle>Acta Parasit</stitle><addtitle>Acta Parasitol</addtitle><date>2018-12-19</date><risdate>2018</risdate><volume>63</volume><issue>4</issue><spage>721</spage><epage>727</epage><pages>721-727</pages><issn>1230-2821</issn><eissn>1896-1851</eissn><abstract>Taenia multiceps
, one of the most widely distributed zoonotic tapeworm parasites, is able to parasitize the small intestine of canids. The metacestode of
T.multiceps
is fatal to ruminants and causes important economic losses in livestock. However, molecular characteristics of
T.multiceps
and coenurus in China are still unclear. In this study, 36 goat isolates of the coenurus stage and 18 dog isolates of the adult stage of
T.multiceps
were obtained from three geographical areas in China and the isolated parasite above were analyzed by amplifying the partial of cytochrome coxidase subunit 1(p
cox
1), 12S ribosomal RNA (12S rRNA) from mitochondrial DNA (mtDNA) regions and an internal transcribed spacer (ITS) of ribosomal DNA (rDNA). These DNA sequences obtained from
T.multiceps
and coenurus were employed to evaluate the nucleotide diversity and confirm the relationship between
T.multiceps
and coenurus. Sequences variation were 0–1.4%, 0–1.5%, 0–4.2% for p
cox
1, 12S rRNA and ITS, respectively, among
T.multiceps
and coenurus isolates obtained in this study. In Sichuan province, sequence variations for
Co-enurus cerebralis
isolated from Yaan city were 0–1.4% for p
cox
1, 0–1.0% for 12S rRNA and 0–2.1% for ITS. In Hunan province, variations were 0–1.0%, 0–1.5% and 0–3.3% for corresponding genes for non-coenurus cerebralis isolated from Changsha city, while variations of
T.multiceps
isolates from Xiangxi autonomous prefecture were 0–1.0%, 0–1.1% and 0–3.4% for p
cox
1, 12S rRNA and ITS, respectively. Phylogenetic analysis based on p
cox
1 sequences indicated that all cerebral and non-cerebral metacestodes belong to
T.multiceps
. These results provide reference values for future molecular epidemiological and biological study on
T.multiceps
in dogs and intermediate hosts.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>30367774</pmid><doi>10.1515/ap-2018-0085</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Acta parasitologica, 2018-12, Vol.63 (4), p.721-727 |
| issn | 1230-2821 1896-1851 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2126917833 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animal Systematics/Taxonomy/Biogeography Animals Biomedical and Life Sciences Biomedicine China Cyclooxygenase 1 - genetics Cytochrome Cytochromes Deoxyribonucleic acid DNA DNA, Helminth - genetics DNA, Helminth - isolation & purification DNA, Intergenic - genetics DNA, Mitochondrial - genetics Dogs Ecology Economic impact Epidemiology Gene sequencing Genetic Markers Genetic Variation Goat Diseases - parasitology Goats internal transcribed spacer (ITS) Livestock Medical Microbiology Microbiology Mitochondrial DNA mitochondrial DNA (mtDNA) Nucleotide sequence Nucleotides Original Paper Parasites Parasitology phylogenetic analysis Phylogenetics Phylogeny Polymerase Chain Reaction - veterinary Ribosomal DNA RNA, Ribosomal - genetics rRNA 12S Sequence Alignment - veterinary Small intestine Spacer Taenia - classification Taenia - genetics Taenia - growth & development Taenia multiceps Taeniasis - parasitology Taeniasis - veterinary Variation |
| title | Molecular characterization and phylogenetic analysis of Taenia multiceps from China |
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