Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin
Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on...
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| Veröffentlicht in: | Monoclonal antibodies in immunodiagnosis and immunotherapy 2018-11, Vol.37 (5), p.233-237 |
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| creator | Furusawa, Yoshikazu Yamada, Shinji Itai, Shunsuke Sano, Masato Nakamura, Takuro Yanaka, Miyuki Handa, Saori Mizuno, Takuya Maeda, Ken Fukui, Masato Harada, Hiroyuki Kaneko, Mika K. Kato, Yukinari |
| description | Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on platelets. Monoclonal antibodies (mAbs) against human-, mouse-, rat-, rabbit-, dog-, bovine-, and cat-PDPN have already been established. However, anti-horse PDPN mAbs have not yet been developed. In this study, we immunized mice with synthetic horse PDPN peptides and developed anti-horse PDPN mAbs. One of the established mAbs, PMab-202 (IgG
1
, kappa), was specifically able to detect horse PDPN in Chinese hamster ovary/horse PDPN (CHO/horPDPN) cells in flow cytometry experiments. PMab-202 was also able to detect endogenous horse PDPN expressed in and a horse kidney cell line, FHK-Tcl3.1, in flow cytometry and Western blot analyses. PMab-202 is expected to prove useful in investigating the function of horse PDPN. |
| doi_str_mv | 10.1089/mab.2018.0030 |
| format | Article |
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1
, kappa), was specifically able to detect horse PDPN in Chinese hamster ovary/horse PDPN (CHO/horPDPN) cells in flow cytometry experiments. PMab-202 was also able to detect endogenous horse PDPN expressed in and a horse kidney cell line, FHK-Tcl3.1, in flow cytometry and Western blot analyses. PMab-202 is expected to prove useful in investigating the function of horse PDPN.</description><identifier>ISSN: 2167-9436</identifier><identifier>EISSN: 2167-9436</identifier><identifier>DOI: 10.1089/mab.2018.0030</identifier><identifier>PMID: 30362932</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc., publishers</publisher><subject>Alveoli ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - genetics ; Antibodies, Monoclonal - immunology ; Antibody Specificity - immunology ; CHO Cells ; Cricetinae ; Cricetulus ; Endothelial cells ; Epitope Mapping ; Epitopes - immunology ; Flow Cytometry ; Glomerulus ; Glycoproteins ; Horses - immunology ; Humans ; Immunization ; Immunoglobulin G ; Kidneys ; Lectins, C-Type - immunology ; Lungs ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - immunology ; Mice ; Monoclonal antibodies ; Peptides ; Platelet aggregation ; Platelet Aggregation - immunology ; Platelets ; Podocytes - immunology ; Short Communications ; Tissues</subject><ispartof>Monoclonal antibodies in immunodiagnosis and immunotherapy, 2018-11, Vol.37 (5), p.233-237</ispartof><rights>2018, Mary Ann Liebert, Inc., publishers</rights><rights>(©) Copyright 2018, Mary Ann Liebert, Inc., publishers</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-6f4af00c507fb98867b1b61d7782ca3a66ce90fd522180dec60d3eecaa20dd23</citedby><cites>FETCH-LOGICAL-c346t-6f4af00c507fb98867b1b61d7782ca3a66ce90fd522180dec60d3eecaa20dd23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30362932$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Furusawa, Yoshikazu</creatorcontrib><creatorcontrib>Yamada, Shinji</creatorcontrib><creatorcontrib>Itai, Shunsuke</creatorcontrib><creatorcontrib>Sano, Masato</creatorcontrib><creatorcontrib>Nakamura, Takuro</creatorcontrib><creatorcontrib>Yanaka, Miyuki</creatorcontrib><creatorcontrib>Handa, Saori</creatorcontrib><creatorcontrib>Mizuno, Takuya</creatorcontrib><creatorcontrib>Maeda, Ken</creatorcontrib><creatorcontrib>Fukui, Masato</creatorcontrib><creatorcontrib>Harada, Hiroyuki</creatorcontrib><creatorcontrib>Kaneko, Mika K.</creatorcontrib><creatorcontrib>Kato, Yukinari</creatorcontrib><title>Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin</title><title>Monoclonal antibodies in immunodiagnosis and immunotherapy</title><addtitle>Monoclon Antib Immunodiagn Immunother</addtitle><description>Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on platelets. Monoclonal antibodies (mAbs) against human-, mouse-, rat-, rabbit-, dog-, bovine-, and cat-PDPN have already been established. However, anti-horse PDPN mAbs have not yet been developed. In this study, we immunized mice with synthetic horse PDPN peptides and developed anti-horse PDPN mAbs. One of the established mAbs, PMab-202 (IgG
1
, kappa), was specifically able to detect horse PDPN in Chinese hamster ovary/horse PDPN (CHO/horPDPN) cells in flow cytometry experiments. PMab-202 was also able to detect endogenous horse PDPN expressed in and a horse kidney cell line, FHK-Tcl3.1, in flow cytometry and Western blot analyses. PMab-202 is expected to prove useful in investigating the function of horse PDPN.</description><subject>Alveoli</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - genetics</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibody Specificity - immunology</subject><subject>CHO Cells</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Endothelial cells</subject><subject>Epitope Mapping</subject><subject>Epitopes - immunology</subject><subject>Flow Cytometry</subject><subject>Glomerulus</subject><subject>Glycoproteins</subject><subject>Horses - immunology</subject><subject>Humans</subject><subject>Immunization</subject><subject>Immunoglobulin G</subject><subject>Kidneys</subject><subject>Lectins, C-Type - immunology</subject><subject>Lungs</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - immunology</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Peptides</subject><subject>Platelet aggregation</subject><subject>Platelet Aggregation - immunology</subject><subject>Platelets</subject><subject>Podocytes - immunology</subject><subject>Short Communications</subject><subject>Tissues</subject><issn>2167-9436</issn><issn>2167-9436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0Eoqh0ZEWRWFhSznZiJ2NVtRSpFR26R_4KpErsYidD_z0JLQixcMudTo9e3T0I3WGYYsjyp0bIKQGcTQEoXKAbghmP84Syy1_zCE1C2ENfHBNM02s0okAZySm5QctFaIWsq_DeGNtGrow2zjpVOyvqaGbbSjp9jLYbIWMCJJq9icqGNlo5H0y0ddodamEre4uuSlEHMzn3MdotF7v5Kl6_Pr_MZ-tY0YS1MSsTUQKoFHgp8yxjXGLJsOY8I0pQwZgyOZQ6JQRnoI1ioKkxSggCWhM6Ro-n2IN3H50JbdFUQZm6v8G4LhQEE5Zjiinr0Yc_6N51vv_qi-KYU0iTnopPlPIuBG_K4uCrRvhjgaEYFBe94mJQXAyKe_7-nNrJxugf-ltoD9ATMKyFtXVlpPHtP7Gf2p2F8g</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Furusawa, Yoshikazu</creator><creator>Yamada, Shinji</creator><creator>Itai, Shunsuke</creator><creator>Sano, Masato</creator><creator>Nakamura, Takuro</creator><creator>Yanaka, Miyuki</creator><creator>Handa, Saori</creator><creator>Mizuno, Takuya</creator><creator>Maeda, Ken</creator><creator>Fukui, Masato</creator><creator>Harada, Hiroyuki</creator><creator>Kaneko, Mika K.</creator><creator>Kato, Yukinari</creator><general>Mary Ann Liebert, Inc., publishers</general><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20181101</creationdate><title>Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin</title><author>Furusawa, Yoshikazu ; Yamada, Shinji ; Itai, Shunsuke ; Sano, Masato ; Nakamura, Takuro ; Yanaka, Miyuki ; Handa, Saori ; Mizuno, Takuya ; Maeda, Ken ; Fukui, Masato ; Harada, Hiroyuki ; Kaneko, Mika K. ; Kato, Yukinari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c346t-6f4af00c507fb98867b1b61d7782ca3a66ce90fd522180dec60d3eecaa20dd23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alveoli</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - genetics</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibody Specificity - immunology</topic><topic>CHO Cells</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Endothelial cells</topic><topic>Epitope Mapping</topic><topic>Epitopes - immunology</topic><topic>Flow Cytometry</topic><topic>Glomerulus</topic><topic>Glycoproteins</topic><topic>Horses - immunology</topic><topic>Humans</topic><topic>Immunization</topic><topic>Immunoglobulin G</topic><topic>Kidneys</topic><topic>Lectins, C-Type - immunology</topic><topic>Lungs</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>Peptides</topic><topic>Platelet aggregation</topic><topic>Platelet Aggregation - immunology</topic><topic>Platelets</topic><topic>Podocytes - immunology</topic><topic>Short Communications</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furusawa, Yoshikazu</creatorcontrib><creatorcontrib>Yamada, Shinji</creatorcontrib><creatorcontrib>Itai, Shunsuke</creatorcontrib><creatorcontrib>Sano, Masato</creatorcontrib><creatorcontrib>Nakamura, Takuro</creatorcontrib><creatorcontrib>Yanaka, Miyuki</creatorcontrib><creatorcontrib>Handa, Saori</creatorcontrib><creatorcontrib>Mizuno, Takuya</creatorcontrib><creatorcontrib>Maeda, Ken</creatorcontrib><creatorcontrib>Fukui, Masato</creatorcontrib><creatorcontrib>Harada, Hiroyuki</creatorcontrib><creatorcontrib>Kaneko, Mika K.</creatorcontrib><creatorcontrib>Kato, Yukinari</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furusawa, Yoshikazu</au><au>Yamada, Shinji</au><au>Itai, Shunsuke</au><au>Sano, Masato</au><au>Nakamura, Takuro</au><au>Yanaka, Miyuki</au><au>Handa, Saori</au><au>Mizuno, Takuya</au><au>Maeda, Ken</au><au>Fukui, Masato</au><au>Harada, Hiroyuki</au><au>Kaneko, Mika K.</au><au>Kato, Yukinari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin</atitle><jtitle>Monoclonal antibodies in immunodiagnosis and immunotherapy</jtitle><addtitle>Monoclon Antib Immunodiagn Immunother</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>37</volume><issue>5</issue><spage>233</spage><epage>237</epage><pages>233-237</pages><issn>2167-9436</issn><eissn>2167-9436</eissn><abstract>Podoplanin (PDPN), a type I transmembrane glycoprotein, is expressed in several body tissues, including podocytes of renal glomerulus, type I alveolar cells of lung, and lymphatic endothelial cells. PDPN activates platelet aggregation by binding to C-type lectin-like receptor-2 (CLEC-2) presented on platelets. Monoclonal antibodies (mAbs) against human-, mouse-, rat-, rabbit-, dog-, bovine-, and cat-PDPN have already been established. However, anti-horse PDPN mAbs have not yet been developed. In this study, we immunized mice with synthetic horse PDPN peptides and developed anti-horse PDPN mAbs. One of the established mAbs, PMab-202 (IgG
1
, kappa), was specifically able to detect horse PDPN in Chinese hamster ovary/horse PDPN (CHO/horPDPN) cells in flow cytometry experiments. PMab-202 was also able to detect endogenous horse PDPN expressed in and a horse kidney cell line, FHK-Tcl3.1, in flow cytometry and Western blot analyses. PMab-202 is expected to prove useful in investigating the function of horse PDPN.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc., publishers</pub><pmid>30362932</pmid><doi>10.1089/mab.2018.0030</doi><tpages>5</tpages></addata></record> |
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| subjects | Alveoli Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - genetics Antibodies, Monoclonal - immunology Antibody Specificity - immunology CHO Cells Cricetinae Cricetulus Endothelial cells Epitope Mapping Epitopes - immunology Flow Cytometry Glomerulus Glycoproteins Horses - immunology Humans Immunization Immunoglobulin G Kidneys Lectins, C-Type - immunology Lungs Membrane Glycoproteins - genetics Membrane Glycoproteins - immunology Mice Monoclonal antibodies Peptides Platelet aggregation Platelet Aggregation - immunology Platelets Podocytes - immunology Short Communications Tissues |
| title | Establishment of Monoclonal Antibody PMab-202 Against Horse Podoplanin |
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